PubMed:4325002 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/4325002","sourcedb":"PubMed","sourceid":"4325002","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/4325002","text":"Phosphorylation of ribosome-associated protein by an adenosine 3':5'-cyclic monophosphate-dependent protein kinase: location of the microsomal receptor and protein kinase.\nIn the adrenal cortex, the adenosine 3':5'-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH(4)Cl retain the substrate of the protein kinase reaction, but are dependent on the NH(4)Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.","tracks":[]}