PubMed:38401165
Annnotations
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 351-362 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 825-836 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 1313-1324 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T4 | 1647-1658 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T16 | 331-346 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T18 | 806-821 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T5 | 1294-1309 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T7 | 1481-1496 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T8 | 1598-1613 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 331-346 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T2 | 351-362 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 806-821 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T4 | 825-836 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T5 | 1294-1309 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T6 | 1313-1324 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T7 | 1481-1496 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T8 | 1598-1613 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T9 | 1647-1658 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 301-310 | OrganismTaxon | denotes | prominent | 37571 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 219-239 | Body_part | denotes | extracellular matrix | http://purl.obolibrary.org/obo/GO_0031012 |
| T2 | 609-614 | Body_part | denotes | Golgi | http://purl.obolibrary.org/obo/GO_0005794 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-123 | Sentence | denotes | Chemo-enzymatic synthesis of tetrasaccharide linker peptides to study the divergent step in glycosaminoglycan biosynthesis. |
| T2 | 124-296 | Sentence | denotes | Glycosaminoglycans are extended linear polysaccharides present on cell surfaces and within the extracellular matrix that play crucial roles in various biological processes. |
| T3 | 297-533 | Sentence | denotes | Two prominent glycosaminoglycans, heparan sulfate and chondroitin sulfate, are covalently linked to proteoglycan core proteins through a common tetrasaccharide linker comprising glucuronic acid, galactose, galactose, and xylose moities. |
| T4 | 534-646 | Sentence | denotes | This tetrasaccharide linker is meticulously assembled step by step by four Golgi-localized glycosyltransferases. |
| T5 | 647-859 | Sentence | denotes | The addition of the fifth sugar moiety, either N-acetylglucosamine or N-acetylgalactosamine, initiates further chain elongation, resulting in the formation of heparan sulfate or chondroitin sulfate, respectively. |
| T6 | 860-993 | Sentence | denotes | Despite the fundamental significance of this step in glycosaminoglycan biosynthesis, its regulatory mechanisms have remained elusive. |
| T7 | 994-1211 | Sentence | denotes | In this study, we detail the expression and purification of the four linker-synthesizing glycosyltransferases and their utilization in the production of fluorescent peptides carrying the native tetrasaccharide linker. |
| T8 | 1212-1347 | Sentence | denotes | We generated five tetrasaccharide peptides, mimicking the core proteins of either heparan sulfate or chondroitin sulfate proteoglycans. |
| T9 | 1348-1510 | Sentence | denotes | These peptides were readily accepted as substrates by the EXTL3 enzyme, which adds an N-acetylglucosamine moiety, thereby initiating heparan sulfate biosynthesis. |
| T10 | 1511-1681 | Sentence | denotes | Importantly, EXTL3 showed a preference towards peptides mimicking the core proteins of heparan sulfate proteoglycans over the ones from chondroitin sulfate proteoglycans. |
| T11 | 1682-1791 | Sentence | denotes | This suggests that EXTL3 could play a role in the decision-making step during glycosaminoglycan biosynthesis. |
| T12 | 1792-2028 | Sentence | denotes | The innovative strategy for chemo-enzymatic synthesis of fluorescent-labeled linker-peptides promises to be instrumental in advancing future investigations into the initial steps and the divergent step of glycosaminoglycan biosynthesis. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 351-362 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 825-836 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 1313-1324 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T4 | 1647-1658 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T16 | 331-346 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T18 | 806-821 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T5 | 1294-1309 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T7 | 1481-1496 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
| T8 | 1598-1613 | Glycan | denotes | heparan sulfate | https://api.glycosmos.org/wurcs2image/latest/png/binary/G17927IW |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 331-346 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T2 | 351-362 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 806-821 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T4 | 825-836 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T5 | 1294-1309 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T6 | 1313-1324 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T7 | 1481-1496 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T8 | 1598-1613 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | heparan sulfate | http://www.glycoepitope.jp/epitopes/EP0086 |
| T9 | 1647-1658 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 301-310 | OrganismTaxon | denotes | prominent | 37571 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 219-239 | Body_part | denotes | extracellular matrix | http://purl.obolibrary.org/obo/GO_0031012 |
| T2 | 609-614 | Body_part | denotes | Golgi | http://purl.obolibrary.org/obo/GO_0005794 |