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Chemical structure and protective effect against alcoholic kidney and heart damages of a novel polysaccharide from Piperis Dahongpao.
In this manuscript, the polysaccharide isolated from Piperis Dahongpao was extracted with the method of water-extraction and alcohol-precipitation. Besides, the homogeneous polysaccharide (PDP-1) with an MW of 2.47 × 103 KDa was purified with the Sephadex G-150 column. The monosaccharide composition analysis showed that PDP-1 consisted of Man, Glc and Gal in a ratio of 1.678:1.784:1, respectively. Besides, the methylation analysis indicated that PDP-1 was composed of six sugar alcohol derivatives, which were →4，6)-D-Galp-(1→, →4)-D-Galp-(1→, →4)-D -Manp-(1→, →4)-D-Glcp-(1→, →6)-D -Glcp-(1→ and D-Glcp-(1 → . Otherwise, the microscopic conformation of PDP-1 was flat, dense and porous. In addition, the PDP-1 had a strong scavenging ability for DPPH free radicals, Hydroxyl free radicals and ABTS free radicals. At the same time, PDP-1 could effectively decrease the peroxidation process of kidney and heart in mice with acute alcoholic liver injury, improve its antioxidant capacity, and protect the organ injury caused by alcohol to a certain extent. Therefore, the results of this manuscript showed that Piperis Dahongpao polysaccharides have antioxidant potential, which lays a theoretical foundation for the research of Piperis Dahongpa polysaccharides in medicine.
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