PubMed:32703420 JSONTXT

{"project":"LitCovid-PD-FMA-UBERON","denotations":[{"id":"T1","span":{"begin":53,"end":60},"obj":"Body_part"},{"id":"T2","span":{"begin":299,"end":306},"obj":"Body_part"},{"id":"T3","span":{"begin":310,"end":317},"obj":"Body_part"},{"id":"T4","span":{"begin":405,"end":413},"obj":"Body_part"},{"id":"T5","span":{"begin":453,"end":458},"obj":"Body_part"},{"id":"T6","span":{"begin":515,"end":523},"obj":"Body_part"},{"id":"T7","span":{"begin":538,"end":543},"obj":"Body_part"},{"id":"T8","span":{"begin":565,"end":572},"obj":"Body_part"},{"id":"T9","span":{"begin":668,"end":675},"obj":"Body_part"},{"id":"T10","span":{"begin":694,"end":701},"obj":"Body_part"},{"id":"T11","span":{"begin":778,"end":783},"obj":"Body_part"},{"id":"T12","span":{"begin":868,"end":876},"obj":"Body_part"},{"id":"T13","span":{"begin":1000,"end":1007},"obj":"Body_part"},{"id":"T14","span":{"begin":1024,"end":1029},"obj":"Body_part"},{"id":"T15","span":{"begin":1074,"end":1081},"obj":"Body_part"},{"id":"T16","span":{"begin":1178,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"fma_id","subj":"T1","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A2","pred":"fma_id","subj":"T2","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A3","pred":"fma_id","subj":"T3","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A4","pred":"fma_id","subj":"T4","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A5","pred":"fma_id","subj":"T5","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A6","pred":"fma_id","subj":"T6","obj":"http://purl.org/sig/ont/fma/fma58616"},{"id":"A7","pred":"fma_id","subj":"T7","obj":"http://purl.org/sig/ont/fma/fma68646"},{"id":"A8","pred":"fma_id","subj":"T8","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A9","pred":"fma_id","subj":"T9","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A10","pred":"fma_id","subj":"T10","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A11","pred":"fma_id","subj":"T11","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A12","pred":"fma_id","subj":"T12","obj":"http://purl.org/sig/ont/fma/fma62871"},{"id":"A13","pred":"fma_id","subj":"T13","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A14","pred":"fma_id","subj":"T14","obj":"http://purl.org/sig/ont/fma/fma63083"},{"id":"A15","pred":"fma_id","subj":"T15","obj":"http://purl.org/sig/ont/fma/fma67257"},{"id":"A16","pred":"fma_id","subj":"T16","obj":"http://purl.org/sig/ont/fma/fma62871"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PD-UBERON","denotations":[{"id":"T1","span":{"begin":453,"end":458},"obj":"Body_part"},{"id":"T2","span":{"begin":778,"end":783},"obj":"Body_part"},{"id":"T3","span":{"begin":1024,"end":1029},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PD-MONDO","denotations":[{"id":"T1","span":{"begin":36,"end":44},"obj":"Disease"},{"id":"T2","span":{"begin":121,"end":134},"obj":"Disease"},{"id":"T3","span":{"begin":224,"end":232},"obj":"Disease"},{"id":"T4","span":{"begin":282,"end":290},"obj":"Disease"},{"id":"T5","span":{"begin":854,"end":862},"obj":"Disease"},{"id":"T6","span":{"begin":987,"end":995},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005108"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0100096"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0005091"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PD-CLO","denotations":[{"id":"T1","span":{"begin":83,"end":91},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_7091"},{"id":"T2","span":{"begin":115,"end":116},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T3","span":{"begin":173,"end":178},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_10239"},{"id":"T4","span":{"begin":249,"end":250},"obj":"http://purl.obolibrary.org/obo/CLO_0001020"},{"id":"T5","span":{"begin":344,"end":352},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_7091"},{"id":"T6","span":{"begin":444,"end":452},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_7091"},{"id":"T7","span":{"begin":515,"end":518},"obj":"http://purl.obolibrary.org/obo/UBERON_0001013"},{"id":"T8","span":{"begin":538,"end":543},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T9","span":{"begin":769,"end":777},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_7091"},{"id":"T10","span":{"begin":865,"end":867},"obj":"http://purl.obolibrary.org/obo/CLO_0008922"},{"id":"T11","span":{"begin":865,"end":867},"obj":"http://purl.obolibrary.org/obo/CLO_0050052"},{"id":"T12","span":{"begin":936,"end":945},"obj":"http://purl.obolibrary.org/obo/NCBITaxon_7091"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PD-CHEBI","denotations":[{"id":"T2","span":{"begin":299,"end":306},"obj":"Chemical"},{"id":"T1","span":{"begin":53,"end":60},"obj":"Chemical"},{"id":"T3","span":{"begin":310,"end":317},"obj":"Chemical"},{"id":"T4","span":{"begin":405,"end":413},"obj":"Chemical"},{"id":"T5","span":{"begin":565,"end":572},"obj":"Chemical"},{"id":"T6","span":{"begin":668,"end":675},"obj":"Chemical"},{"id":"T7","span":{"begin":694,"end":701},"obj":"Chemical"},{"id":"T8","span":{"begin":804,"end":813},"obj":"Chemical"},{"id":"T9","span":{"begin":865,"end":867},"obj":"Chemical"},{"id":"T10","span":{"begin":1000,"end":1007},"obj":"Chemical"},{"id":"T11","span":{"begin":1074,"end":1081},"obj":"Chemical"},{"id":"T12","span":{"begin":1149,"end":1156},"obj":"Chemical"}],"attributes":[{"id":"A1","pred":"chebi_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A2","pred":"chebi_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A3","pred":"chebi_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A4","pred":"chebi_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A5","pred":"chebi_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A6","pred":"chebi_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A7","pred":"chebi_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A8","pred":"chebi_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CHEBI_132554"},{"id":"A9","pred":"chebi_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CHEBI_29387"},{"id":"A10","pred":"chebi_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A11","pred":"chebi_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CHEBI_36080"},{"id":"A12","pred":"chebi_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/CHEBI_59132"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PD-GO-BP","denotations":[{"id":"T1","span":{"begin":1178,"end":1203},"obj":"http://purl.obolibrary.org/obo/GO_0002377"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-PubTator","denotations":[{"id":"2","span":{"begin":36,"end":46},"obj":"Species"},{"id":"3","span":{"begin":83,"end":91},"obj":"Species"},{"id":"19","span":{"begin":121,"end":134},"obj":"Disease"},{"id":"20","span":{"begin":224,"end":232},"obj":"Disease"},{"id":"21","span":{"begin":282,"end":292},"obj":"Species"},{"id":"22","span":{"begin":293,"end":298},"obj":"Gene"},{"id":"23","span":{"begin":308,"end":317},"obj":"Gene"},{"id":"24","span":{"begin":344,"end":352},"obj":"Species"},{"id":"25","span":{"begin":444,"end":452},"obj":"Species"},{"id":"26","span":{"begin":563,"end":572},"obj":"Gene"},{"id":"27","span":{"begin":692,"end":701},"obj":"Gene"},{"id":"28","span":{"begin":769,"end":777},"obj":"Species"},{"id":"29","span":{"begin":854,"end":864},"obj":"Species"},{"id":"30","span":{"begin":936,"end":945},"obj":"Species"},{"id":"31","span":{"begin":987,"end":997},"obj":"Species"},{"id":"32","span":{"begin":998,"end":1007},"obj":"Gene"},{"id":"33","span":{"begin":1072,"end":1081},"obj":"Gene"}],"attributes":[{"id":"A2","pred":"tao:has_database_id","subj":"2","obj":"Tax:2697049"},{"id":"A3","pred":"tao:has_database_id","subj":"3","obj":"Tax:7091"},{"id":"A19","pred":"tao:has_database_id","subj":"19","obj":"MESH:D001102"},{"id":"A20","pred":"tao:has_database_id","subj":"20","obj":"MESH:C000657245"},{"id":"A21","pred":"tao:has_database_id","subj":"21","obj":"Tax:2697049"},{"id":"A22","pred":"tao:has_database_id","subj":"22","obj":"Gene:43740568"},{"id":"A23","pred":"tao:has_database_id","subj":"23","obj":"Gene:43740568"},{"id":"A24","pred":"tao:has_database_id","subj":"24","obj":"Tax:7091"},{"id":"A25","pred":"tao:has_database_id","subj":"25","obj":"Tax:7091"},{"id":"A26","pred":"tao:has_database_id","subj":"26","obj":"Gene:43740568"},{"id":"A27","pred":"tao:has_database_id","subj":"27","obj":"Gene:43740568"},{"id":"A28","pred":"tao:has_database_id","subj":"28","obj":"Tax:7091"},{"id":"A29","pred":"tao:has_database_id","subj":"29","obj":"Tax:2697049"},{"id":"A30","pred":"tao:has_database_id","subj":"30","obj":"Tax:7091"},{"id":"A31","pred":"tao:has_database_id","subj":"31","obj":"Tax:2697049"},{"id":"A32","pred":"tao:has_database_id","subj":"32","obj":"Gene:43740568"},{"id":"A33","pred":"tao:has_database_id","subj":"33","obj":"Gene:43740568"}],"namespaces":[{"prefix":"Tax","uri":"https://www.ncbi.nlm.nih.gov/taxonomy/"},{"prefix":"MESH","uri":"https://id.nlm.nih.gov/mesh/"},{"prefix":"Gene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"CVCL","uri":"https://web.expasy.org/cellosaurus/CVCL_"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid-sentences","denotations":[{"id":"T1","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"T2","span":{"begin":100,"end":219},"obj":"Sentence"},{"id":"T3","span":{"begin":220,"end":371},"obj":"Sentence"},{"id":"T4","span":{"begin":372,"end":544},"obj":"Sentence"},{"id":"T5","span":{"begin":545,"end":687},"obj":"Sentence"},{"id":"T6","span":{"begin":688,"end":877},"obj":"Sentence"},{"id":"T7","span":{"begin":878,"end":1048},"obj":"Sentence"},{"id":"T8","span":{"begin":1049,"end":1218},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":36,"end":46},"obj":"Species:2697049"},{"id":"T10","span":{"begin":563,"end":572},"obj":"Gene:7448"},{"id":"T11","span":{"begin":692,"end":701},"obj":"Gene:7448"},{"id":"T12","span":{"begin":769,"end":777},"obj":"Species:7091"},{"id":"T13","span":{"begin":854,"end":864},"obj":"Species:2697049"},{"id":"T14","span":{"begin":936,"end":945},"obj":"Species:7091"},{"id":"T15","span":{"begin":987,"end":997},"obj":"Species:2697049"},{"id":"T16","span":{"begin":998,"end":1007},"obj":"Gene:7448"},{"id":"T17","span":{"begin":1072,"end":1081},"obj":"Gene:7448"},{"id":"T2","span":{"begin":83,"end":91},"obj":"Species:7091"},{"id":"T3","span":{"begin":121,"end":134},"obj":"Disease:MESH:D001102"},{"id":"T4","span":{"begin":224,"end":232},"obj":"Disease:MESH:C000657245"},{"id":"T5","span":{"begin":282,"end":292},"obj":"Species:2697049"},{"id":"T6","span":{"begin":293,"end":298},"obj":"Gene:43740568"},{"id":"T7","span":{"begin":308,"end":317},"obj":"Gene:7448"},{"id":"T8","span":{"begin":344,"end":352},"obj":"Species:7091"},{"id":"T9","span":{"begin":444,"end":452},"obj":"Species:7091"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"LitCovid_AGAC_only","denotations":[{"id":"p64037s3","span":{"begin":563,"end":564},"obj":"Protein"},{"id":"p64037s4","span":{"begin":565,"end":572},"obj":"Protein"},{"id":"p64037s20","span":{"begin":651,"end":655},"obj":"NegReg"},{"id":"p64037s21","span":{"begin":656,"end":686},"obj":"MPA"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":36,"end":46},"obj":"Species:2697049"},{"id":"T2","span":{"begin":83,"end":91},"obj":"Species:7091"},{"id":"T3","span":{"begin":121,"end":134},"obj":"Disease:MESH:D001102"},{"id":"T4","span":{"begin":224,"end":232},"obj":"Disease:MESH:C000657245"},{"id":"T5","span":{"begin":282,"end":292},"obj":"Species:2697049"},{"id":"T6","span":{"begin":293,"end":298},"obj":"Gene:43740568"},{"id":"T7","span":{"begin":308,"end":317},"obj":"Gene:7448"},{"id":"T8","span":{"begin":344,"end":352},"obj":"Species:7091"},{"id":"T9","span":{"begin":444,"end":452},"obj":"Species:7091"},{"id":"T10","span":{"begin":563,"end":572},"obj":"Gene:7448"},{"id":"T11","span":{"begin":692,"end":701},"obj":"Gene:7448"},{"id":"T12","span":{"begin":769,"end":777},"obj":"Species:7091"},{"id":"T13","span":{"begin":854,"end":864},"obj":"Species:2697049"},{"id":"T14","span":{"begin":936,"end":945},"obj":"Species:7091"},{"id":"T15","span":{"begin":987,"end":997},"obj":"Species:2697049"},{"id":"T16","span":{"begin":998,"end":1007},"obj":"Gene:7448"},{"id":"T17","span":{"begin":1072,"end":1081},"obj":"Gene:7448"}],"text":"Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.\nIn the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were successfully secreted to silkworm serum, whereas those formed insoluble structure in the larval fat body and the pupal cells. The ectodomain of S protein with the native sequence was cleaved by the host furin-protease, resulting in less recombinant protein production. The S protein modified in furin protease-target site was efficiently secreted to silkworm serum and was purified as oligomers, which showed immunoreactivity for anti-SARS-CoV-2 S2 antibody. By using the direct transfection of recombinant bacmid to silkworms, we achieved the efficient production of SARS-CoV-2 S protein as fetal bovine serum (FBS)-free system. The resultant purified S protein would be useful tools for the development of immunodetection kits, antigen for immunization for immunoglobulin production, and vaccines."}