PubMed:30789901 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/30789901","sourcedb":"PubMed","sourceid":"30789901","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/30789901","text":"Global transcriptional regulation of innate immunity by ATF-7 in C. elegans.\nThe nematode Caenorhabditis elegans has emerged as a genetically tractable animal host in which to study evolutionarily conserved mechanisms of innate immune signaling. We previously showed that the PMK-1 p38 mitogen-activated protein kinase (MAPK) pathway regulates innate immunity of C. elegans through phosphorylation of the CREB/ATF bZIP transcription factor, ATF-7. Here, we have undertaken a genomic analysis of the transcriptional response of C. elegans to infection by Pseudomonas aeruginosa, combining genome-wide expression analysis by RNA-seq with ATF-7 chromatin immunoprecipitation followed by sequencing (ChIP-Seq). We observe that PMK-1-ATF-7 activity regulates a majority of all genes induced by pathogen infection, and observe ATF-7 occupancy in regulatory regions of pathogen-induced genes in a PMK-1-dependent manner. Moreover, functional analysis of a subset of these ATF-7-regulated pathogen-induced target genes supports a direct role for this transcriptional response in host defense. The genome-wide regulation through PMK-1- ATF-7 signaling reveals a striking level of control over the innate immune response to infection through a single transcriptional regulator.","tracks":[{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":77,"end":245},"obj":"DRI_Background"},{"id":"T2","span":{"begin":246,"end":404},"obj":"DRI_Outcome"},{"id":"T3","span":{"begin":419,"end":447},"obj":"DRI_Outcome"},{"id":"T4","span":{"begin":707,"end":913},"obj":"DRI_Challenge"},{"id":"T5","span":{"begin":914,"end":964},"obj":"DRI_Approach"},{"id":"T6","span":{"begin":998,"end":1084},"obj":"DRI_Approach"},{"id":"T7","span":{"begin":1085,"end":1119},"obj":"DRI_Background"},{"id":"T8","span":{"begin":1133,"end":1267},"obj":"DRI_Background"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T2","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T3","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T4","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T5","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T6","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T7","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T8","pred":"source","obj":"PubMed_ArguminSci"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_ArguminSci","color":"#ea93ec","default":true}]}]}}