PubMed:28676123 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/28676123","sourcedb":"PubMed","sourceid":"28676123","text":"Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds.\nBACKGROUND: The prevalence of chronic skin wounds in humans is high, and treatment is often complicated by the presence of pathogenic bacteria. Therefore, safe and innovative treatments to reduce the bacterial load in cutaneous wounds are needed. Mesenchymal stromal cells (MSC) are known to provide paracrine signals that act on resident skin cells to promote wound healing, but their potential antibacterial activities are not well described. The present study was designed to examine the antibacterial properties of MSC from horses, as this animal model offers a readily translatable model for MSC therapies in humans. Specifically, we aimed to (i) evaluate the in vitro effects of equine MSC on the growth of representative gram-negative and gram-positive bacterial species commonly found in skin wounds and (ii) define the mechanisms by which MSC inhibit bacterial growth.\nMETHODS: MSC were isolated from the peripheral blood of healthy horses. Gram-negative E. coli and gram-positive S. aureus were cultured in the presence of MSC and MSC conditioned medium (CM), containing all factors secreted by MSC. Bacterial growth was measured by plating bacteria and counting viable colonies or by reading the absorbance of bacterial cultures. Bacterial membrane damage was detected by incorporation of N-phenyl-1-naphthylamine (NPN). Antimicrobial peptide (AMP) gene and protein expression by equine MSC were determined by RT-PCR and Western blot analysis, respectively. Blocking of AMP activity of MSC CM was achieved using AMP-specific antibodies.\nRESULTS: We found that equine MSC and MSC CM inhibit the growth of E. coli and S. aureus, and that MSC CM depolarizes the cell membranes of these bacteria. In addition, we found that equine MSC CM contains AMPs, and blocking these AMPs with antibodies reduces the effects of MSC CM on bacteria.\nCONCLUSIONS: Our results demonstrate that equine MSC inhibit bacterial growth and secrete factors that compromise the membrane integrity of bacteria commonly found in skin wounds. We also identified four specific AMPs produced by equine MSC. The secretion of AMPs may contribute to the value of MSC as a therapy for cutaneous wounds in both horses and humans.","tracks":[{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T1","span":{"begin":142,"end":1007},"obj":"BACKGROUND"},{"id":"T2","span":{"begin":1017,"end":1677},"obj":"METHODS"},{"id":"T3","span":{"begin":1687,"end":1972},"obj":"RESULTS"},{"id":"T4","span":{"begin":1986,"end":2332},"obj":"CONCLUSIONS"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T2","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T3","pred":"source","obj":"PubMed_Structured_Abstracts"},{"subj":"T4","pred":"source","obj":"PubMed_Structured_Abstracts"}]},{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T1","span":{"begin":540,"end":550},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T2","span":{"begin":658,"end":664},"obj":"CVCL_9124|Undefined_cell_line_type|Cricetulus griseus"},{"id":"T3","span":{"begin":694,"end":695},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T4","span":{"begin":766,"end":768},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T5","span":{"begin":1072,"end":1078},"obj":"CVCL_9124|Undefined_cell_line_type|Cricetulus griseus"},{"id":"T6","span":{"begin":1195,"end":1197},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T7","span":{"begin":1273,"end":1280},"obj":"CVCL_B488|Transformed_cell_line|Homo sapiens"},{"id":"T8","span":{"begin":1615,"end":1623},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T9","span":{"begin":1631,"end":1633},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T10","span":{"begin":1687,"end":1689},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T11","span":{"begin":1720,"end":1722},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T12","span":{"begin":1781,"end":1783},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T13","span":{"begin":1847,"end":1849},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T14","span":{"begin":1872,"end":1874},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T15","span":{"begin":1957,"end":1959},"obj":"CVCL_H246|Cancer_cell_line|Homo sapiens"},{"id":"T16","span":{"begin":2153,"end":2155},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T17","span":{"begin":2237,"end":2240},"obj":"CVCL_E773|Transformed_cell_line|Homo sapiens"},{"id":"T18","span":{"begin":2275,"end":2276},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T19","span":{"begin":2314,"end":2320},"obj":"CVCL_9124|Undefined_cell_line_type|Cricetulus griseus"}],"attributes":[{"subj":"T1","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T2","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T3","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T4","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T5","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T6","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T7","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T8","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T9","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T10","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T11","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T12","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T13","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T14","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T15","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T16","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T17","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T18","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T19","pred":"source","obj":"Goldhamster2_Cellosaurus"}]},{"project":"GoldHamster","denotations":[{"id":"T1","span":{"begin":0,"end":13},"obj":"CHEBI:33281"},{"id":"T2","span":{"begin":0,"end":22},"obj":"B3EWR6"},{"id":"T3","span":{"begin":0,"end":22},"obj":"O24006"},{"id":"T4","span":{"begin":14,"end":22},"obj":"D010455"},{"id":"T5","span":{"begin":14,"end":22},"obj":"D010455"},{"id":"T6","span":{"begin":14,"end":22},"obj":"CHEBI:16670"},{"id":"T7","span":{"begin":35,"end":41},"obj":"9796"},{"id":"T8","span":{"begin":42,"end":67},"obj":"CL:0000134"},{"id":"T9","span":{"begin":117,"end":121},"obj":"UBERON:0002199"},{"id":"T10","span":{"begin":117,"end":121},"obj":"UBERON:0000014"},{"id":"T11","span":{"begin":117,"end":121},"obj":"UBERON:0001003"},{"id":"T12","span":{"begin":117,"end":121},"obj":"UBERON:0002097"},{"id":"T13","span":{"begin":168,"end":172},"obj":"UBERON:0002199"},{"id":"T14","span":{"begin":168,"end":172},"obj":"UBERON:0000014"},{"id":"T15","span":{"begin":168,"end":172},"obj":"UBERON:0001003"},{"id":"T16","span":{"begin":168,"end":172},"obj":"UBERON:0002097"},{"id":"T17","span":{"begin":377,"end":402},"obj":"CL:0000134"},{"id":"T18","span":{"begin":404,"end":407},"obj":"PR:000010664"},{"id":"T19","span":{"begin":404,"end":407},"obj":"PR:O60682"},{"id":"T20","span":{"begin":404,"end":407},"obj":"PR:O88940"},{"id":"T21","span":{"begin":404,"end":407},"obj":"CL:0000134"},{"id":"T22","span":{"begin":469,"end":473},"obj":"UBERON:0002199"},{"id":"T23","span":{"begin":469,"end":473},"obj":"UBERON:0000014"},{"id":"T24","span":{"begin":469,"end":473},"obj":"UBERON:0001003"},{"id":"T25","span":{"begin":469,"end":473},"obj":"UBERON:0002097"},{"id":"T26","span":{"begin":491,"end":504},"obj":"GO:0042060"},{"id":"T27","span":{"begin":649,"end":652},"obj":"PR:000010664"},{"id":"T28","span":{"begin":649,"end":652},"obj":"PR:O60682"},{"id":"T29","span":{"begin":649,"end":652},"obj":"PR:O88940"},{"id":"T30","span":{"begin":649,"end":652},"obj":"CL:0000134"},{"id":"T31","span":{"begin":674,"end":680},"obj":"UBERON:3010325"},{"id":"T32","span":{"begin":674,"end":680},"obj":"UBERON:0000468"},{"id":"T33","span":{"begin":727,"end":730},"obj":"PR:000010664"},{"id":"T34","span":{"begin":727,"end":730},"obj":"PR:O60682"},{"id":"T35","span":{"begin":727,"end":730},"obj":"PR:O88940"},{"id":"T36","span":{"begin":727,"end":730},"obj":"CL:0000134"},{"id":"T37","span":{"begin":815,"end":821},"obj":"9796"},{"id":"T38","span":{"begin":822,"end":825},"obj":"PR:000010664"},{"id":"T39","span":{"begin":822,"end":825},"obj":"PR:O60682"},{"id":"T40","span":{"begin":822,"end":825},"obj":"PR:O88940"},{"id":"T41","span":{"begin":822,"end":825},"obj":"CL:0000134"},{"id":"T42","span":{"begin":926,"end":930},"obj":"UBERON:0002199"},{"id":"T43","span":{"begin":926,"end":930},"obj":"UBERON:0000014"},{"id":"T44","span":{"begin":926,"end":930},"obj":"UBERON:0001003"},{"id":"T45","span":{"begin":926,"end":930},"obj":"UBERON:0002097"},{"id":"T46","span":{"begin":978,"end":981},"obj":"PR:000010664"},{"id":"T47","span":{"begin":978,"end":981},"obj":"PR:O60682"},{"id":"T48","span":{"begin":978,"end":981},"obj":"PR:O88940"},{"id":"T49","span":{"begin":978,"end":981},"obj":"CL:0000134"},{"id":"T50","span":{"begin":1017,"end":1020},"obj":"PR:000010664"},{"id":"T51","span":{"begin":1017,"end":1020},"obj":"PR:O60682"},{"id":"T52","span":{"begin":1017,"end":1020},"obj":"PR:O88940"},{"id":"T53","span":{"begin":1017,"end":1020},"obj":"CL:0000134"},{"id":"T54","span":{"begin":1094,"end":1101},"obj":"D004926"},{"id":"T55","span":{"begin":1094,"end":1101},"obj":"562"},{"id":"T56","span":{"begin":1163,"end":1166},"obj":"PR:000010664"},{"id":"T57","span":{"begin":1163,"end":1166},"obj":"PR:O60682"},{"id":"T58","span":{"begin":1163,"end":1166},"obj":"PR:O88940"},{"id":"T59","span":{"begin":1163,"end":1166},"obj":"CL:0000134"},{"id":"T60","span":{"begin":1171,"end":1174},"obj":"PR:000010664"},{"id":"T61","span":{"begin":1171,"end":1174},"obj":"PR:O60682"},{"id":"T62","span":{"begin":1171,"end":1174},"obj":"PR:O88940"},{"id":"T63","span":{"begin":1171,"end":1174},"obj":"CL:0000134"},{"id":"T64","span":{"begin":1175,"end":1193},"obj":"D017077"},{"id":"T65","span":{"begin":1235,"end":1238},"obj":"PR:000010664"},{"id":"T66","span":{"begin":1235,"end":1238},"obj":"PR:O60682"},{"id":"T67","span":{"begin":1235,"end":1238},"obj":"PR:O88940"},{"id":"T68","span":{"begin":1235,"end":1238},"obj":"CL:0000134"},{"id":"T69","span":{"begin":1381,"end":1389},"obj":"UBERON:0000094"},{"id":"T71","span":{"begin":1381,"end":1389},"obj":"UBERON:0000158"},{"id":"T70","span":{"begin":1381,"end":1389},"obj":"GO:0016020"},{"id":"T72","span":{"begin":1430,"end":1454},"obj":"C005444"},{"id":"T73","span":{"begin":1430,"end":1454},"obj":"C005444"},{"id":"T74","span":{"begin":1430,"end":1454},"obj":"CHEBI:34876"},{"id":"T80","span":{"begin":1462,"end":1475},"obj":"CHEBI:33281"},{"id":"T81","span":{"begin":1476,"end":1483},"obj":"SO:0001064"},{"id":"T82","span":{"begin":1476,"end":1483},"obj":"PR:000018264"},{"id":"T84","span":{"begin":1476,"end":1483},"obj":"PR:000018263"},{"id":"T83","span":{"begin":1476,"end":1483},"obj":"CHEBI:16670"},{"id":"T85","span":{"begin":1485,"end":1488},"obj":"D000249"},{"id":"T86","span":{"begin":1485,"end":1488},"obj":"D000249"},{"id":"T87","span":{"begin":1485,"end":1488},"obj":"CHEBI:456215"},{"id":"T88","span":{"begin":1485,"end":1488},"obj":"CHEBI:16027"},{"id":"T89","span":{"begin":1485,"end":1488},"obj":"CHEBI:28971"},{"id":"T90","span":{"begin":1490,"end":1494},"obj":"SO:0000704"},{"id":"T95","span":{"begin":1499,"end":1506},"obj":"SO:0000104"},{"id":"T94","span":{"begin":1499,"end":1506},"obj":"PR:000000001"},{"id":"T92","span":{"begin":1499,"end":1506},"obj":"CHEBI:36080"},{"id":"T93","span":{"begin":1499,"end":1506},"obj":"CHEBI:11122"},{"id":"T91","span":{"begin":1499,"end":1506},"obj":"GO:0003675"},{"id":"T96","span":{"begin":1521,"end":1527},"obj":"9796"},{"id":"T97","span":{"begin":1528,"end":1531},"obj":"PR:000010664"},{"id":"T98","span":{"begin":1528,"end":1531},"obj":"PR:O60682"},{"id":"T99","span":{"begin":1528,"end":1531},"obj":"PR:O88940"},{"id":"T100","span":{"begin":1528,"end":1531},"obj":"CL:0000134"},{"id":"T101","span":{"begin":1554,"end":1557},"obj":"Q41249"},{"id":"T102","span":{"begin":1554,"end":1557},"obj":"O66148"},{"id":"T103","span":{"begin":1554,"end":1557},"obj":"Q59987"},{"id":"T104","span":{"begin":1554,"end":1557},"obj":"Q01289"},{"id":"T105","span":{"begin":1554,"end":1557},"obj":"O80333"},{"id":"T106","span":{"begin":1554,"end":1557},"obj":"P15904"},{"id":"T107","span":{"begin":1554,"end":1557},"obj":"Q9SDT1"},{"id":"T108","span":{"begin":1554,"end":1557},"obj":"Q39617"},{"id":"T109","span":{"begin":1611,"end":1614},"obj":"D000249"},{"id":"T110","span":{"begin":1611,"end":1614},"obj":"D000249"},{"id":"T111","span":{"begin":1611,"end":1614},"obj":"CHEBI:456215"},{"id":"T112","span":{"begin":1611,"end":1614},"obj":"CHEBI:16027"},{"id":"T113","span":{"begin":1611,"end":1614},"obj":"CHEBI:28971"},{"id":"T114","span":{"begin":1627,"end":1630},"obj":"PR:000010664"},{"id":"T115","span":{"begin":1627,"end":1630},"obj":"PR:O60682"},{"id":"T116","span":{"begin":1627,"end":1630},"obj":"PR:O88940"},{"id":"T117","span":{"begin":1627,"end":1630},"obj":"CL:0000134"},{"id":"T118","span":{"begin":1653,"end":1656},"obj":"D000249"},{"id":"T119","span":{"begin":1653,"end":1656},"obj":"D000249"},{"id":"T120","span":{"begin":1653,"end":1656},"obj":"CHEBI:456215"},{"id":"T121","span":{"begin":1653,"end":1656},"obj":"CHEBI:16027"},{"id":"T122","span":{"begin":1653,"end":1656},"obj":"CHEBI:28971"},{"id":"T123","span":{"begin":1666,"end":1676},"obj":"D000906"},{"id":"T124","span":{"begin":1701,"end":1707},"obj":"9796"},{"id":"T125","span":{"begin":1708,"end":1711},"obj":"PR:000010664"},{"id":"T126","span":{"begin":1708,"end":1711},"obj":"PR:O60682"},{"id":"T127","span":{"begin":1708,"end":1711},"obj":"PR:O88940"},{"id":"T128","span":{"begin":1708,"end":1711},"obj":"CL:0000134"},{"id":"T129","span":{"begin":1716,"end":1719},"obj":"PR:000010664"},{"id":"T130","span":{"begin":1716,"end":1719},"obj":"PR:O60682"},{"id":"T131","span":{"begin":1716,"end":1719},"obj":"PR:O88940"},{"id":"T132","span":{"begin":1716,"end":1719},"obj":"CL:0000134"},{"id":"T133","span":{"begin":1745,"end":1752},"obj":"D004926"},{"id":"T134","span":{"begin":1745,"end":1752},"obj":"562"},{"id":"T135","span":{"begin":1777,"end":1780},"obj":"PR:000010664"},{"id":"T136","span":{"begin":1777,"end":1780},"obj":"PR:O60682"},{"id":"T137","span":{"begin":1777,"end":1780},"obj":"PR:O88940"},{"id":"T138","span":{"begin":1777,"end":1780},"obj":"CL:0000134"},{"id":"T139","span":{"begin":1861,"end":1867},"obj":"9796"},{"id":"T143","span":{"begin":1868,"end":1871},"obj":"CL:0000134"},{"id":"T140","span":{"begin":1868,"end":1871},"obj":"PR:000010664"},{"id":"T141","span":{"begin":1868,"end":1871},"obj":"PR:O60682"},{"id":"T142","span":{"begin":1868,"end":1871},"obj":"PR:O88940"},{"id":"T144","span":{"begin":1884,"end":1888},"obj":"PR:P30566"},{"id":"T145","span":{"begin":1884,"end":1888},"obj":"PR:000003794"},{"id":"T146","span":{"begin":1909,"end":1913},"obj":"PR:P30566"},{"id":"T147","span":{"begin":1909,"end":1913},"obj":"PR:000003794"},{"id":"T148","span":{"begin":1919,"end":1929},"obj":"D000906"},{"id":"T152","span":{"begin":1953,"end":1956},"obj":"CL:0000134"},{"id":"T149","span":{"begin":1953,"end":1956},"obj":"PR:000010664"},{"id":"T150","span":{"begin":1953,"end":1956},"obj":"PR:O60682"},{"id":"T151","span":{"begin":1953,"end":1956},"obj":"PR:O88940"},{"id":"T153","span":{"begin":2015,"end":2021},"obj":"9796"},{"id":"T157","span":{"begin":2022,"end":2025},"obj":"CL:0000134"},{"id":"T154","span":{"begin":2022,"end":2025},"obj":"PR:000010664"},{"id":"T155","span":{"begin":2022,"end":2025},"obj":"PR:O60682"},{"id":"T156","span":{"begin":2022,"end":2025},"obj":"PR:O88940"},{"id":"T159","span":{"begin":2091,"end":2099},"obj":"GO:0016020"},{"id":"T158","span":{"begin":2091,"end":2099},"obj":"UBERON:0000094"},{"id":"T160","span":{"begin":2091,"end":2099},"obj":"UBERON:0000158"},{"id":"T161","span":{"begin":2140,"end":2144},"obj":"UBERON:0002199"},{"id":"T162","span":{"begin":2140,"end":2144},"obj":"UBERON:0000014"},{"id":"T163","span":{"begin":2140,"end":2144},"obj":"UBERON:0001003"},{"id":"T164","span":{"begin":2140,"end":2144},"obj":"UBERON:0002097"},{"id":"T165","span":{"begin":2186,"end":2190},"obj":"PR:P30566"},{"id":"T166","span":{"begin":2186,"end":2190},"obj":"PR:000003794"},{"id":"T167","span":{"begin":2203,"end":2209},"obj":"9796"},{"id":"T168","span":{"begin":2210,"end":2213},"obj":"PR:000010664"},{"id":"T169","span":{"begin":2210,"end":2213},"obj":"PR:O60682"},{"id":"T170","span":{"begin":2210,"end":2213},"obj":"PR:O88940"},{"id":"T171","span":{"begin":2210,"end":2213},"obj":"CL:0000134"},{"id":"T173","span":{"begin":2219,"end":2228},"obj":"UBERON:0000456"},{"id":"T172","span":{"begin":2219,"end":2228},"obj":"GO:0046903"},{"id":"T174","span":{"begin":2232,"end":2236},"obj":"PR:P30566"},{"id":"T175","span":{"begin":2232,"end":2236},"obj":"PR:000003794"},{"id":"T176","span":{"begin":2268,"end":2271},"obj":"PR:000010664"},{"id":"T177","span":{"begin":2268,"end":2271},"obj":"PR:O60682"},{"id":"T178","span":{"begin":2268,"end":2271},"obj":"PR:O88940"},{"id":"T179","span":{"begin":2268,"end":2271},"obj":"CL:0000134"}],"attributes":[{"subj":"T1","pred":"source","obj":"GoldHamster"},{"subj":"T2","pred":"source","obj":"GoldHamster"},{"subj":"T3","pred":"source","obj":"GoldHamster"},{"subj":"T4","pred":"source","obj":"GoldHamster"},{"subj":"T5","pred":"source","obj":"GoldHamster"},{"subj":"T6","pred":"source","obj":"GoldHamster"},{"subj":"T7","pred":"source","obj":"GoldHamster"},{"subj":"T8","pred":"source","obj":"GoldHamster"},{"subj":"T9","pred":"source","obj":"GoldHamster"},{"subj":"T10","pred":"source","obj":"GoldHamster"},{"subj":"T11","pred":"source","obj":"GoldHamster"},{"subj":"T12","pred":"source","obj":"GoldHamster"},{"subj":"T13","pred":"source","obj":"GoldHamster"},{"subj":"T14","pred":"source","obj":"GoldHamster"},{"subj":"T15","pred":"source","obj":"GoldHamster"},{"subj":"T16","pred":"source","obj":"GoldHamster"},{"subj":"T17","pred":"source","obj":"GoldHamster"},{"subj":"T18","pred":"source","obj":"GoldHamster"},{"subj":"T19","pred":"source","obj":"GoldHamster"},{"subj":"T20","pred":"source","obj":"GoldHamster"},{"subj":"T21","pred":"source","obj":"GoldHamster"},{"subj":"T22","pred":"source","obj":"GoldHamster"},{"subj":"T23","pred":"source","obj":"GoldHamster"},{"subj":"T24","pred":"source","obj":"GoldHamster"},{"subj":"T25","pred":"source","obj":"GoldHamster"},{"subj":"T26","pred":"source","obj":"GoldHamster"},{"subj":"T27","pred":"source","obj":"GoldHamster"},{"subj":"T28","pred":"source","obj":"GoldHamster"},{"subj":"T29","pred":"source","obj":"GoldHamster"},{"subj":"T30","pred":"source","obj":"GoldHamster"},{"subj":"T31","pred":"source","obj":"GoldHamster"},{"subj":"T32","pred":"source","obj":"GoldHamster"},{"subj":"T33","pred":"source","obj":"GoldHamster"},{"subj":"T34","pred":"source","obj":"GoldHamster"},{"subj":"T35","pred":"source","obj":"GoldHamster"},{"subj":"T36","pred":"source","obj":"GoldHamster"},{"subj":"T37","pred":"source","obj":"GoldHamster"},{"subj":"T38","pred":"source","obj":"GoldHamster"},{"subj":"T39","pred":"source","obj":"GoldHamster"},{"subj":"T40","pred":"source","obj":"GoldHamster"},{"subj":"T41","pred":"source","obj":"GoldHamster"},{"subj":"T42","pred":"source","obj":"GoldHamster"},{"subj":"T43","pred":"source","obj":"GoldHamster"},{"subj":"T44","pred":"source","obj":"GoldHamster"},{"subj":"T45","pred":"source","obj":"GoldHamster"},{"subj":"T46","pred":"source","obj":"GoldHamster"},{"subj":"T47","pred":"source","obj":"GoldHamster"},{"subj":"T48","pred":"source","obj":"GoldHamster"},{"subj":"T49","pred":"source","obj":"GoldHamster"},{"subj":"T50","pred":"source","obj":"GoldHamster"},{"subj":"T51","pred":"source","obj":"GoldHamster"},{"subj":"T52","pred":"source","obj":"GoldHamster"},{"subj":"T53","pred":"source","obj":"GoldHamster"},{"subj":"T54","pred":"source","obj":"GoldHamster"},{"subj":"T55","pred":"source","obj":"GoldHamster"},{"subj":"T56","pred":"source","obj":"GoldHamster"},{"subj":"T57","pred":"source","obj":"GoldHamster"},{"subj":"T58","pred":"source","obj":"GoldHamster"},{"subj":"T59","pred":"source","obj":"GoldHamster"},{"subj":"T60","pred":"source","obj":"GoldHamster"},{"subj":"T61","pred":"source","obj":"GoldHamster"},{"subj":"T62","pred":"source","obj":"GoldHamster"},{"subj":"T63","pred":"source","obj":"GoldHamster"},{"subj":"T64","pred":"source","obj":"GoldHamster"},{"subj":"T65","pred":"source","obj":"GoldHamster"},{"subj":"T66","pred":"source","obj":"GoldHamster"},{"subj":"T67","pred":"source","obj":"GoldHamster"},{"subj":"T68","pred":"source","obj":"GoldHamster"},{"subj":"T69","pred":"source","obj":"GoldHamster"},{"subj":"T71","pred":"source","obj":"GoldHamster"},{"subj":"T70","pred":"source","obj":"GoldHamster"},{"subj":"T72","pred":"source","obj":"GoldHamster"},{"subj":"T73","pred":"source","obj":"GoldHamster"},{"subj":"T74","pred":"source","obj":"GoldHamster"},{"subj":"T80","pred":"source","obj":"GoldHamster"},{"subj":"T81","pred":"source","obj":"GoldHamster"},{"subj":"T82","pred":"source","obj":"GoldHamster"},{"subj":"T84","pred":"source","obj":"GoldHamster"},{"subj":"T83","pred":"source","obj":"GoldHamster"},{"subj":"T85","pred":"source","obj":"GoldHamster"},{"subj":"T86","pred":"source","obj":"GoldHamster"},{"subj":"T87","pred":"source","obj":"GoldHamster"},{"subj":"T88","pred":"source","obj":"GoldHamster"},{"subj":"T89","pred":"source","obj":"GoldHamster"},{"subj":"T90","pred":"source","obj":"GoldHamster"},{"subj":"T95","pred":"source","obj":"GoldHamster"},{"subj":"T94","pred":"source","obj":"GoldHamster"},{"subj":"T92","pred":"source","obj":"GoldHamster"},{"subj":"T93","pred":"source","obj":"GoldHamster"},{"subj":"T91","pred":"source","obj":"GoldHamster"},{"subj":"T96","pred":"source","obj":"GoldHamster"},{"subj":"T97","pred":"source","obj":"GoldHamster"},{"subj":"T98","pred":"source","obj":"GoldHamster"},{"subj":"T99","pred":"source","obj":"GoldHamster"},{"subj":"T100","pred":"source","obj":"GoldHamster"},{"subj":"T101","pred":"source","obj":"GoldHamster"},{"subj":"T102","pred":"source","obj":"GoldHamster"},{"subj":"T103","pred":"source","obj":"GoldHamster"},{"subj":"T104","pred":"source","obj":"GoldHamster"},{"subj":"T105","pred":"source","obj":"GoldHamster"},{"subj":"T106","pred":"source","obj":"GoldHamster"},{"subj":"T107","pred":"source","obj":"GoldHamster"},{"subj":"T108","pred":"source","obj":"GoldHamster"},{"subj":"T109","pred":"source","obj":"GoldHamster"},{"subj":"T110","pred":"source","obj":"GoldHamster"},{"subj":"T111","pred":"source","obj":"GoldHamster"},{"subj":"T112","pred":"source","obj":"GoldHamster"},{"subj":"T113","pred":"source","obj":"GoldHamster"},{"subj":"T114","pred":"source","obj":"GoldHamster"},{"subj":"T115","pred":"source","obj":"GoldHamster"},{"subj":"T116","pred":"source","obj":"GoldHamster"},{"subj":"T117","pred":"source","obj":"GoldHamster"},{"subj":"T118","pred":"source","obj":"GoldHamster"},{"subj":"T119","pred":"source","obj":"GoldHamster"},{"subj":"T120","pred":"source","obj":"GoldHamster"},{"subj":"T121","pred":"source","obj":"GoldHamster"},{"subj":"T122","pred":"source","obj":"GoldHamster"},{"subj":"T123","pred":"source","obj":"GoldHamster"},{"subj":"T124","pred":"source","obj":"GoldHamster"},{"subj":"T125","pred":"source","obj":"GoldHamster"},{"subj":"T126","pred":"source","obj":"GoldHamster"},{"subj":"T127","pred":"source","obj":"GoldHamster"},{"subj":"T128","pred":"source","obj":"GoldHamster"},{"subj":"T129","pred":"source","obj":"GoldHamster"},{"subj":"T130","pred":"source","obj":"GoldHamster"},{"subj":"T131","pred":"source","obj":"GoldHamster"},{"subj":"T132","pred":"source","obj":"GoldHamster"},{"subj":"T133","pred":"source","obj":"GoldHamster"},{"subj":"T134","pred":"source","obj":"GoldHamster"},{"subj":"T135","pred":"source","obj":"GoldHamster"},{"subj":"T136","pred":"source","obj":"GoldHamster"},{"subj":"T137","pred":"source","obj":"GoldHamster"},{"subj":"T138","pred":"source","obj":"GoldHamster"},{"subj":"T139","pred":"source","obj":"GoldHamster"},{"subj":"T143","pred":"source","obj":"GoldHamster"},{"subj":"T140","pred":"source","obj":"GoldHamster"},{"subj":"T141","pred":"source","obj":"GoldHamster"},{"subj":"T142","pred":"source","obj":"GoldHamster"},{"subj":"T144","pred":"source","obj":"GoldHamster"},{"subj":"T145","pred":"source","obj":"GoldHamster"},{"subj":"T146","pred":"source","obj":"GoldHamster"},{"subj":"T147","pred":"source","obj":"GoldHamster"},{"subj":"T148","pred":"source","obj":"GoldHamster"},{"subj":"T152","pred":"source","obj":"GoldHamster"},{"subj":"T149","pred":"source","obj":"GoldHamster"},{"subj":"T150","pred":"source","obj":"GoldHamster"},{"subj":"T151","pred":"source","obj":"GoldHamster"},{"subj":"T153","pred":"source","obj":"GoldHamster"},{"subj":"T157","pred":"source","obj":"GoldHamster"},{"subj":"T154","pred":"source","obj":"GoldHamster"},{"subj":"T155","pred":"source","obj":"GoldHamster"},{"subj":"T156","pred":"source","obj":"GoldHamster"},{"subj":"T159","pred":"source","obj":"GoldHamster"},{"subj":"T158","pred":"source","obj":"GoldHamster"},{"subj":"T160","pred":"source","obj":"GoldHamster"},{"subj":"T161","pred":"source","obj":"GoldHamster"},{"subj":"T162","pred":"source","obj":"GoldHamster"},{"subj":"T163","pred":"source","obj":"GoldHamster"},{"subj":"T164","pred":"source","obj":"GoldHamster"},{"subj":"T165","pred":"source","obj":"GoldHamster"},{"subj":"T166","pred":"source","obj":"GoldHamster"},{"subj":"T167","pred":"source","obj":"GoldHamster"},{"subj":"T168","pred":"source","obj":"GoldHamster"},{"subj":"T169","pred":"source","obj":"GoldHamster"},{"subj":"T170","pred":"source","obj":"GoldHamster"},{"subj":"T171","pred":"source","obj":"GoldHamster"},{"subj":"T173","pred":"source","obj":"GoldHamster"},{"subj":"T172","pred":"source","obj":"GoldHamster"},{"subj":"T174","pred":"source","obj":"GoldHamster"},{"subj":"T175","pred":"source","obj":"GoldHamster"},{"subj":"T176","pred":"source","obj":"GoldHamster"},{"subj":"T177","pred":"source","obj":"GoldHamster"},{"subj":"T178","pred":"source","obj":"GoldHamster"},{"subj":"T179","pred":"source","obj":"GoldHamster"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_Structured_Abstracts","color":"#93ec96","default":true},{"id":"Goldhamster2_Cellosaurus","color":"#ec93a9"},{"id":"GoldHamster","color":"#93c4ec"}]}]}}