PubMed:28545510 / 394-1296 JSONTXT

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    PubMed_Structured_Abstracts

    {"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T2","span":{"begin":0,"end":902},"obj":"METHODS"}],"text":"Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2.5 × 10(5)/100 μL media with the BM-MSCs contained within the insert well), MSC-Sp solution, or naive culture media (control) for 72 h. A linear defect in confluent cell cultures was created (i.e., corneal scratch assay) to assess the cellular closure (\"healing\") over time. Three representative areas of the scratch in each culture were photographed at each time point and the scratch area was quantitated using image analysis software (ImageJ). Media from the scratches were analyzed for various growth factors using human enzyme-linked immunosorbent assay (ELISA) kits that crossreact with the horse."}

    Goldhamster2_Cellosaurus

    {"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T9","span":{"begin":0,"end":4},"obj":"CVCL_D953|Induced_pluripotent_stem_cell|Homo sapiens"},{"id":"T10","span":{"begin":70,"end":76},"obj":"CVCL_9124|Undefined_cell_line_type|Cricetulus griseus"},{"id":"T11","span":{"begin":118,"end":120},"obj":"CVCL_E481|Transformed_cell_line|Homo sapiens"},{"id":"T12","span":{"begin":118,"end":120},"obj":"CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer"},{"id":"T13","span":{"begin":230,"end":236},"obj":"CVCL_B488|Transformed_cell_line|Homo sapiens"},{"id":"T14","span":{"begin":259,"end":261},"obj":"CVCL_8754|Cancer_cell_line|Homo sapiens"},{"id":"T15","span":{"begin":259,"end":261},"obj":"CVCL_H241|Cancer_cell_line|Homo sapiens"},{"id":"T16","span":{"begin":273,"end":275},"obj":"CVCL_E481|Transformed_cell_line|Homo sapiens"},{"id":"T17","span":{"begin":273,"end":275},"obj":"CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer"},{"id":"T18","span":{"begin":332,"end":334},"obj":"CVCL_E481|Transformed_cell_line|Homo sapiens"},{"id":"T19","span":{"begin":332,"end":334},"obj":"CVCL_S926|Spontaneously_immortalized_cell_line|Lates calcarifer"},{"id":"T20","span":{"begin":379,"end":381},"obj":"CVCL_1Y11|Transformed_cell_line|Homo sapiens"},{"id":"T21","span":{"begin":379,"end":381},"obj":"CVCL_L986|Undefined_cell_line_type|Aotus trivirgatus"},{"id":"T22","span":{"begin":435,"end":436},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T23","span":{"begin":568,"end":572},"obj":"CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens"},{"id":"T24","span":{"begin":658,"end":662},"obj":"CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens"},{"id":"T25","span":{"begin":896,"end":901},"obj":"CVCL_9124|Undefined_cell_line_type|Cricetulus griseus"}],"text":"Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2.5 × 10(5)/100 μL media with the BM-MSCs contained within the insert well), MSC-Sp solution, or naive culture media (control) for 72 h. A linear defect in confluent cell cultures was created (i.e., corneal scratch assay) to assess the cellular closure (\"healing\") over time. Three representative areas of the scratch in each culture were photographed at each time point and the scratch area was quantitated using image analysis software (ImageJ). Media from the scratches were analyzed for various growth factors using human enzyme-linked immunosorbent assay (ELISA) kits that crossreact with the horse."}

    GoldHamster

    {"project":"GoldHamster","denotations":[{"id":"T26","span":{"begin":0,"end":11},"obj":"UBERON:0002371"},{"id":"T25","span":{"begin":0,"end":11},"obj":"D001855"},{"id":"T27","span":{"begin":0,"end":11},"obj":"D001855"},{"id":"T29","span":{"begin":121,"end":125},"obj":"PR:000023297"},{"id":"T30","span":{"begin":121,"end":125},"obj":"PR:P0C0S1"},{"id":"T28","span":{"begin":121,"end":125},"obj":"GO:0043854"},{"id":"T31","span":{"begin":276,"end":279},"obj":"PR:000010664"},{"id":"T32","span":{"begin":276,"end":279},"obj":"PR:O60682"},{"id":"T33","span":{"begin":276,"end":279},"obj":"PR:O88940"},{"id":"T34","span":{"begin":276,"end":279},"obj":"CL:0000134"},{"id":"T36","span":{"begin":335,"end":339},"obj":"PR:000023297"},{"id":"T37","span":{"begin":335,"end":339},"obj":"PR:P0C0S1"},{"id":"T35","span":{"begin":335,"end":339},"obj":"GO:0043854"},{"id":"T38","span":{"begin":361,"end":367},"obj":"SO:0000046"},{"id":"T39","span":{"begin":375,"end":378},"obj":"PR:000010664"},{"id":"T40","span":{"begin":375,"end":378},"obj":"PR:O60682"},{"id":"T41","span":{"begin":375,"end":378},"obj":"PR:O88940"},{"id":"T42","span":{"begin":375,"end":378},"obj":"CL:0000134"},{"id":"T43","span":{"begin":382,"end":390},"obj":"CHEBI:75958"},{"id":"T44","span":{"begin":401,"end":414},"obj":"D003470"},{"id":"T45","span":{"begin":437,"end":443},"obj":"SO:0000987"},{"id":"T46","span":{"begin":797,"end":811},"obj":"D036341"},{"id":"T47","span":{"begin":797,"end":811},"obj":"D036341"},{"id":"T48","span":{"begin":818,"end":823},"obj":"D006801"},{"id":"T49","span":{"begin":896,"end":901},"obj":"D006736"},{"id":"T50","span":{"begin":896,"end":901},"obj":"9796"},{"id":"T51","span":{"begin":896,"end":901},"obj":"CVCL_9124"}],"text":"Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2.5 × 10(5)/100 μL media with the BM-MSCs contained within the insert well), MSC-Sp solution, or naive culture media (control) for 72 h. A linear defect in confluent cell cultures was created (i.e., corneal scratch assay) to assess the cellular closure (\"healing\") over time. Three representative areas of the scratch in each culture were photographed at each time point and the scratch area was quantitated using image analysis software (ImageJ). Media from the scratches were analyzed for various growth factors using human enzyme-linked immunosorbent assay (ELISA) kits that crossreact with the horse."}