PubMed:28545510 / 394-1296 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T2	0-902	METHODS	denotes	Bone marrow aspirates and eyes were collected from normal, euthanized horses with subsequent isolation and culture of BM-MSCs and corneal stromal cells. Corneal stromal cells were culture-expanded in the culture well of transwell plates and then treated with an autologous BM-MSC suspension (dose: 2.5 × 10(5)/100 μL media with the BM-MSCs contained within the insert well), MSC-Sp solution, or naive culture media (control) for 72 h. A linear defect in confluent cell cultures was created (i.e., corneal scratch assay) to assess the cellular closure ("healing") over time. Three representative areas of the scratch in each culture were photographed at each time point and the scratch area was quantitated using image analysis software (ImageJ). Media from the scratches were analyzed for various growth factors using human enzyme-linked immunosorbent assay (ELISA) kits that crossreact with the horse.

Id	Subject	Object	Predicate	Lexical cue
T9	0-4	CVCL_D953\|Induced_pluripotent_stem_cell\|Homo sapiens	denotes	Bone
T10	70-76	CVCL_9124\|Undefined_cell_line_type\|Cricetulus griseus	denotes	horses
T11	118-120	CVCL_E481\|Transformed_cell_line\|Homo sapiens	denotes	BM
T12	118-120	CVCL_S926\|Spontaneously_immortalized_cell_line\|Lates calcarifer	denotes	BM
T13	230-236	CVCL_B488\|Transformed_cell_line\|Homo sapiens	denotes	plates
T14	259-261	CVCL_8754\|Cancer_cell_line\|Homo sapiens	denotes	an
T15	259-261	CVCL_H241\|Cancer_cell_line\|Homo sapiens	denotes	an
T16	273-275	CVCL_E481\|Transformed_cell_line\|Homo sapiens	denotes	BM
T17	273-275	CVCL_S926\|Spontaneously_immortalized_cell_line\|Lates calcarifer	denotes	BM
T18	332-334	CVCL_E481\|Transformed_cell_line\|Homo sapiens	denotes	BM
T19	332-334	CVCL_S926\|Spontaneously_immortalized_cell_line\|Lates calcarifer	denotes	BM
T20	379-381	CVCL_1Y11\|Transformed_cell_line\|Homo sapiens	denotes	Sp
T21	379-381	CVCL_L986\|Undefined_cell_line_type\|Aotus trivirgatus	denotes	Sp
T22	435-436	CVCL_6479\|Finite_cell_line\|Mus musculus	denotes	A
T23	568-572	CVCL_0047\|Telomerase_immortalized_cell_line\|Homo sapiens	denotes	time
T24	658-662	CVCL_0047\|Telomerase_immortalized_cell_line\|Homo sapiens	denotes	time
T25	896-901	CVCL_9124\|Undefined_cell_line_type\|Cricetulus griseus	denotes	horse

Id	Subject	Object	Predicate	Lexical cue
T26	0-11	UBERON:0002371	denotes	Bone marrow
T25	0-11	D001855	denotes	Bone marrow
T27	0-11	D001855	denotes	Bone marrow
T29	121-125	PR:000023297	denotes	MSCs
T30	121-125	PR:P0C0S1	denotes	MSCs
T28	121-125	GO:0043854	denotes	MSCs
T31	276-279	PR:000010664	denotes	MSC
T32	276-279	PR:O60682	denotes	MSC
T33	276-279	PR:O88940	denotes	MSC
T34	276-279	CL:0000134	denotes	MSC
T36	335-339	PR:000023297	denotes	MSCs
T37	335-339	PR:P0C0S1	denotes	MSCs
T35	335-339	GO:0043854	denotes	MSCs
T38	361-367	SO:0000046	denotes	insert
T39	375-378	PR:000010664	denotes	MSC
T40	375-378	PR:O60682	denotes	MSC
T41	375-378	PR:O88940	denotes	MSC
T42	375-378	CL:0000134	denotes	MSC
T43	382-390	CHEBI:75958	denotes	solution
T44	401-414	D003470	denotes	culture media
T45	437-443	SO:0000987	denotes	linear
T46	797-811	D036341	denotes	growth factors
T47	797-811	D036341	denotes	growth factors
T48	818-823	D006801	denotes	human
T49	896-901	D006736	denotes	horse
T50	896-901	9796	denotes	horse
T51	896-901	CVCL_9124	denotes	horse