PubMed:2787334 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2787334","sourcedb":"PubMed","sourceid":"2787334","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2787334","text":"Reliable confirmation of antibodies to human immunodeficiency virus type 1 (HIV-1) with an enzyme-linked immunoassay using recombinant antigens derived from the HIV-1 gag, pol, and env genes.\nAn enzyme-linked immunoassay (ELISA) using six recombinant proteins corresponding to large segments of the human immunodeficiency virus type 1 (HIV-1) gag, pol, and env gene products (HIVAGEN; SmithKline Bio-Science Laboratories, Van Nuys, Calif.) was developed to confirm the presence of antibodies to HIV-1 in sera reactive in the whole-cell-derived virion screening ELISAs. Serum samples for testing were obtained from healthy seronegative blood donors and from the different categories of HIV-infected individuals (asymptomatic, acquired immunodeficiency syndrome [AIDS]-related complex, and AIDS). A positive reaction was defined as reactivity against an env and at least one other (either gag or pol) HIV-1 gene product; negative was defined as no reaction with any antigen; and indeterminate was defined as reactivity with gag or pol (or both) or with env alone. None of the 1,180 serum samples from healthy seronegative blood donors gave a positive result, and only 49 of these samples (4%) gave indeterminate results. The recombinant HIV-1 antigen ELISA panel identified seropositive individuals with a high degree of accuracy, as a positive reaction was seen with 99.3% of asymptomatic healthy seropositive individuals, 98.1% of patients with AIDS-related complex, and 90.4% of patients with AIDS. None of the 725 HIV-1-seropositive subjects had a negative test result. Reactivity with the Kp41 antigen, corresponding to an amino-terminal portion of the gp41 envelope glycoprotein, by itself demonstrated 100% sensitivity and specificity in distinguishing seronegative from seropositive sera. A subset of seronegative and seropositive samples were tested both with the recombinant HIV-1 antigen ELISA panel and by Western blot (Du Pont Co.). The recombinant HIV-1 antigen ELISA panel accurately identified more seropositive and seronegative samples and had fewer indeterminate results than did Western blot (interpreted by Du Pont criteria).","tracks":[{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":635,"end":640},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":1120,"end":1125},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":569,"end":574},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":1080,"end":1085},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T3","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T4","pred":"source","obj":"performance-test"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":305,"end":321},"obj":"HP_0002721"},{"id":"T2","span":{"begin":734,"end":750},"obj":"HP_0002721"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":357,"end":360},"obj":"gene:100775105"},{"id":"T1","span":{"begin":495,"end":498},"obj":"disease:C0019693"},{"id":"T2","span":{"begin":357,"end":360},"obj":"gene:30816"},{"id":"T3","span":{"begin":495,"end":498},"obj":"disease:C0019693"},{"id":"T4","span":{"begin":357,"end":360},"obj":"gene:100616444"},{"id":"T5","span":{"begin":495,"end":498},"obj":"disease:C0019693"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":191},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":192,"end":568},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":569,"end":794},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":795,"end":1061},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":1062,"end":1218},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1219,"end":1499},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1500,"end":1571},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1572,"end":1794},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1795,"end":1943},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1944,"end":2143},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":191},"obj":"Sentence"},{"id":"T2","span":{"begin":192,"end":568},"obj":"Sentence"},{"id":"T3","span":{"begin":569,"end":794},"obj":"Sentence"},{"id":"T4","span":{"begin":795,"end":1061},"obj":"Sentence"},{"id":"T5","span":{"begin":1062,"end":1218},"obj":"Sentence"},{"id":"T6","span":{"begin":1219,"end":1499},"obj":"Sentence"},{"id":"T7","span":{"begin":1500,"end":1571},"obj":"Sentence"},{"id":"T8","span":{"begin":1572,"end":1794},"obj":"Sentence"},{"id":"T9","span":{"begin":1795,"end":1943},"obj":"Sentence"},{"id":"T10","span":{"begin":1944,"end":2143},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"}]},{"project":"UBERON-AE","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":635,"end":640},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":1120,"end":1125},"obj":"http: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