PubMed:27509880 JSON TXT

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LitCoin-entities

LitCoin-sentences

{"project":"LitCoin-sentences","denotations":[{"id":"T1","span":{"begin":0,"end":211},"obj":"Sentence"},{"id":"T2","span":{"begin":212,"end":372},"obj":"Sentence"},{"id":"T3","span":{"begin":373,"end":670},"obj":"Sentence"},{"id":"T4","span":{"begin":671,"end":863},"obj":"Sentence"},{"id":"T5","span":{"begin":864,"end":1162},"obj":"Sentence"},{"id":"T6","span":{"begin":1163,"end":1383},"obj":"Sentence"},{"id":"T7","span":{"begin":1384,"end":1587},"obj":"Sentence"},{"id":"T8","span":{"begin":1588,"end":1826},"obj":"Sentence"},{"id":"T9","span":{"begin":1827,"end":1996},"obj":"Sentence"},{"id":"T10","span":{"begin":1997,"end":2222},"obj":"Sentence"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-entities-OrganismTaxon-PD

{"project":"LitCoin-entities-OrganismTaxon-PD","denotations":[{"id":"T1","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":535,"end":540},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":2115,"end":2120},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"NCBItxid:9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"NCBItxid:9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"NCBItxid:9606"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin_Mondo

{"project":"LitCoin_Mondo","denotations":[{"id":"T1","span":{"begin":190,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":547,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":2127,"end":2133},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0004992"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0004992"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0004992"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-GeneOrGeneProduct-v0

LitCoin-GeneOrGeneProduct-v2

LitCoin-Disease-MeSH

{"project":"LitCoin-Disease-MeSH","denotations":[{"id":"T1","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":541,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":1052,"end":1057},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":2121,"end":2133},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"originalLabel","subj":"T1","obj":"D003110"},{"id":"A2","pred":"originalLabel","subj":"T2","obj":"D003110"},{"id":"A3","pred":"originalLabel","subj":"T3","obj":"D003643"},{"id":"A4","pred":"originalLabel","subj":"T4","obj":"D003110"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-GeneOrGeneProduct-v3

{"project":"LitCoin-GeneOrGeneProduct-v3","denotations":[{"id":"T1","span":{"begin":19,"end":38},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":93,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T3","span":{"begin":163,"end":172},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":248,"end":267},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":269,"end":273},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":454,"end":458},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":802,"end":811},"obj":"GeneOrGeneProduct"},{"id":"T8","span":{"begin":851,"end":860},"obj":"GeneOrGeneProduct"},{"id":"T9","span":{"begin":1139,"end":1148},"obj":"GeneOrGeneProduct"},{"id":"T10","span":{"begin":1261,"end":1275},"obj":"GeneOrGeneProduct"},{"id":"T11","span":{"begin":1288,"end":1332},"obj":"GeneOrGeneProduct"},{"id":"T12","span":{"begin":1538,"end":1550},"obj":"GeneOrGeneProduct"},{"id":"T13","span":{"begin":1569,"end":1580},"obj":"GeneOrGeneProduct"},{"id":"T14","span":{"begin":1708,"end":1720},"obj":"GeneOrGeneProduct"},{"id":"T15","span":{"begin":1764,"end":1768},"obj":"GeneOrGeneProduct"},{"id":"T16","span":{"begin":1773,"end":1778},"obj":"GeneOrGeneProduct"},{"id":"T17","span":{"begin":1897,"end":1909},"obj":"GeneOrGeneProduct"},{"id":"T18","span":{"begin":2193,"end":2205},"obj":"GeneOrGeneProduct"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin_Mondo_095

{"project":"LitCoin_Mondo_095","denotations":[{"id":"T1","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":541,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":1334,"end":1336},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":1371,"end":1373},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":2121,"end":2133},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0009271"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0021063"},{"id":"A5","pred":"mondo_id","subj":"T4","obj":"0005575"},{"id":"A6","pred":"mondo_id","subj":"T4","obj":"0002032"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0021063"},{"id":"A10","pred":"mondo_id","subj":"T9","obj":"0005575"},{"id":"A11","pred":"mondo_id","subj":"T9","obj":"0002032"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0009271"},{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0021063"},{"id":"A2","pred":"mondo_id","subj":"T1","obj":"0005575"},{"id":"A3","pred":"mondo_id","subj":"T1","obj":"0002032"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-MeSH-Disease-2

{"project":"LitCoin-MeSH-Disease-2","denotations":[{"id":"T1","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":541,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":1052,"end":1057},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":2121,"end":2133},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"ID:","subj":"T1","obj":"D003110"},{"id":"A2","pred":"ID:","subj":"T2","obj":"D003110"},{"id":"A3","pred":"ID:","subj":"T3","obj":"D003643"},{"id":"A4","pred":"ID:","subj":"T4","obj":"D003110"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-MONDO_bioort2019

{"project":"LitCoin-MONDO_bioort2019","denotations":[{"id":"T1","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":541,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":2121,"end":2133},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"#label","subj":"T1","obj":"D003110"},{"id":"A2","pred":"#label","subj":"T2","obj":"D003110"},{"id":"A3","pred":"#label","subj":"T3","obj":"D003110"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin_CellLine

{"project":"LitCoin_CellLine","denotations":[{"id":"T1","span":{"begin":197,"end":204},"obj":"CellLine"},{"id":"T2","span":{"begin":571,"end":578},"obj":"CellLine"},{"id":"T3","span":{"begin":580,"end":584},"obj":"CellLine"},{"id":"T5","span":{"begin":590,"end":594},"obj":"CellLine"}],"attributes":[{"id":"A1","pred":"cellosaurus_accession_id","subj":"T1","obj":"CVCL_0291"},{"id":"A2","pred":"cellosaurus_accession_id","subj":"T2","obj":"CVCL_0291"},{"id":"A3","pred":"cellosaurus_accession_id","subj":"T3","obj":"CVCL_0320"},{"id":"A4","pred":"cellosaurus_accession_id","subj":"T3","obj":"CVCL_A8EZ"},{"id":"A5","pred":"cellosaurus_accession_id","subj":"T5","obj":"CVCL_1724"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-Chemical-MeSH-CHEBI

LitCoin-NCBITaxon-2

{"project":"LitCoin-NCBITaxon-2","denotations":[{"id":"T1","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":535,"end":540},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":2115,"end":2120},"obj":"OrganismTaxon"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

LitCoin-training-merged

biored-valid

biored-valid-deepseek-nr-ng

biored-valid-deepseek-nr-g

biored-valid-deepseek-r-ng

{"project":"biored-valid-deepseek-r-ng","denotations":[{"id":"T1","span":{"begin":19,"end":38},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":49,"end":61},"obj":"ChemicalEntity"},{"id":"T3","span":{"begin":66,"end":80},"obj":"ChemicalEntity"},{"id":"T4","span":{"begin":93,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":139,"end":148},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":163,"end":174},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":197,"end":204},"obj":"CellLine"},{"id":"T10","span":{"begin":524,"end":528},"obj":"ChemicalEntity"},{"id":"T11","span":{"begin":580,"end":584},"obj":"CellLine"},{"id":"T12","span":{"begin":590,"end":600},"obj":"CellLine"},{"id":"T13","span":{"begin":802,"end":813},"obj":"GeneOrGeneProduct"},{"id":"T14","span":{"begin":1538,"end":1556},"obj":"GeneOrGeneProduct"},{"id":"T15","span":{"begin":1569,"end":1586},"obj":"GeneOrGeneProduct"},{"id":"T16","span":{"begin":1764,"end":1768},"obj":"GeneOrGeneProduct"},{"id":"T17","span":{"begin":1773,"end":1778},"obj":"GeneOrGeneProduct"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

biored-valid-deepseek-r-g

{"project":"biored-valid-deepseek-r-g","denotations":[{"id":"T1","span":{"begin":19,"end":38},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":49,"end":61},"obj":"ChemicalEntity"},{"id":"T3","span":{"begin":66,"end":80},"obj":"ChemicalEntity"},{"id":"T4","span":{"begin":93,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":139,"end":142},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":163,"end":174},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":197,"end":204},"obj":"CellLine"},{"id":"T10","span":{"begin":269,"end":273},"obj":"GeneOrGeneProduct"},{"id":"T11","span":{"begin":524,"end":528},"obj":"ChemicalEntity"},{"id":"T12","span":{"begin":580,"end":584},"obj":"CellLine"},{"id":"T13","span":{"begin":590,"end":594},"obj":"CellLine"},{"id":"T14","span":{"begin":821,"end":825},"obj":"ChemicalEntity"},{"id":"T15","span":{"begin":947,"end":959},"obj":"ChemicalEntity"},{"id":"T16","span":{"begin":1322,"end":1325},"obj":"GeneOrGeneProduct"},{"id":"T17","span":{"begin":1538,"end":1550},"obj":"GeneOrGeneProduct"},{"id":"T18","span":{"begin":1569,"end":1586},"obj":"GeneOrGeneProduct"},{"id":"T19","span":{"begin":1764,"end":1768},"obj":"GeneOrGeneProduct"},{"id":"T20","span":{"begin":1773,"end":1778},"obj":"GeneOrGeneProduct"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

biored-valid-gemini-nr-ng

biored-valid-gemini-r-ng

biored-valid-gemini-r-g

biored-valid-gpt-nr-ng

{"project":"biored-valid-gpt-nr-ng","denotations":[{"id":"T1","span":{"begin":49,"end":61},"obj":"ChemicalEntity"},{"id":"T2","span":{"begin":66,"end":80},"obj":"ChemicalEntity"},{"id":"T3","span":{"begin":93,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":139,"end":142},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":163,"end":174},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":197,"end":204},"obj":"CellLine"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}

biored-valid-gpt-r-g

biored-valid-gpt-nr-g

biored-valid-gpt-r-ng

biored-valid-gemini-nr-g

biored-valid-gpt-r-m

biored-valid-gemini-r-m

biored-valid-deepseek-r-m

{"project":"biored-valid-deepseek-r-m","denotations":[{"id":"T1","span":{"begin":19,"end":38},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":49,"end":61},"obj":"ChemicalEntity"},{"id":"T3","span":{"begin":66,"end":80},"obj":"ChemicalEntity"},{"id":"T4","span":{"begin":93,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":139,"end":142},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":163,"end":174},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":178,"end":183},"obj":"OrganismTaxon"},{"id":"T8","span":{"begin":184,"end":196},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":197,"end":204},"obj":"CellLine"},{"id":"T10","span":{"begin":269,"end":273},"obj":"GeneOrGeneProduct"},{"id":"T11","span":{"begin":437,"end":449},"obj":"ChemicalEntity"},{"id":"T12","span":{"begin":454,"end":458},"obj":"GeneOrGeneProduct"},{"id":"T13","span":{"begin":490,"end":495},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T14","span":{"begin":524,"end":528},"obj":"ChemicalEntity"},{"id":"T15","span":{"begin":547,"end":553},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T16","span":{"begin":580,"end":584},"obj":"CellLine"},{"id":"T17","span":{"begin":590,"end":594},"obj":"CellLine"},{"id":"T18","span":{"begin":700,"end":704},"obj":"ChemicalEntity"},{"id":"T19","span":{"begin":724,"end":736},"obj":"ChemicalEntity"},{"id":"T20","span":{"begin":939,"end":943},"obj":"ChemicalEntity"},{"id":"T21","span":{"begin":947,"end":959},"obj":"ChemicalEntity"},{"id":"T22","span":{"begin":1538,"end":1550},"obj":"GeneOrGeneProduct"},{"id":"T23","span":{"begin":1764,"end":1768},"obj":"GeneOrGeneProduct"},{"id":"T24","span":{"begin":1773,"end":1778},"obj":"GeneOrGeneProduct"}],"text":"Combination of the histone deacetylase inhibitor depsipeptide and 5-fluorouracil upregulates major histocompatibility complex class II and p21 genes and activates caspase-3/7 in human colon cancer HCT-116 cells.\nEpigenetic anticancer drugs such as histone deacetylase (HDAC) inhibitors have been combined with existing anticancer drugs for synergistic or additive effects. In the present study, we found that a very low concentration of depsipeptide, an HDAC inhibitor, potentiated the antitumor activity of 5-fluorouracil (5-FU) in a human colon cancer cell model using HCT-116, HT29, and SW48 cells via the inhibition of colony formation ability or cellular viability. Exposure to a combination of 5-FU (1.75 µM) and 1 nM depsipeptide for 24 and 48 h resulted in a 3- to 4-fold increase in activated caspase-3/7, while 5-FU alone failed to activate caspase-3/7. Microarray and subsequent gene ontology analyses revealed that compared to 5-FU or depsipeptide alone, the combination treatment of 5-FU and depsipeptide upregulated genes related to cell death and the apoptotic process consistent with the inhibition of colony formation and caspase-3/7 activation. These analyses indicated marked upregulation of antigen processing and presentation of peptide or polysaccharide antigen via major histocompatibility complex (MHC) class (GO:0002504) and MHC protein complex (GO:0042611). Compared with vehicle controls, the cells treated with the combination of 5-FU and depsipeptide showed marked induction (3- to 8.5-fold) of expression of MHC class II genes, but not of MHC class I genes. Furthermore, our global analysis of gene expression, which was focused on genes involved in the molecular regulation of MHC class II genes, showed enhancement of pro-apoptotic PCAF and CIITA after the combination of 5-FU and depsipeptide. These results may indicate a closer relationship between elevation of MHC class II expression and cellular apoptosis induced by the combination of depsipeptide and 5-FU. To the best of our knowledge, this is the first study to report that the combination of 5-FU and depsipeptide induces human colon cancer cell apoptosis in a concerted manner with the induction of MHC class II gene expression."}