PubMed:26215578 / 608-1124 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T98	0-4	PR:000007171	denotes	EREG
T99	0-4	PR:O14944	denotes	EREG
T100	0-4	PR:Q61521	denotes	EREG
T101	0-4	PR:Q9Z0L5	denotes	EREG
T102	60-65	D006801	denotes	human
T105	77-101	D002285	denotes	ductal carcinoma in situ
T106	77-101	D002285	denotes	ductal carcinoma in situ
T112	103-107	CVCL_5552	denotes	DCIS
T111	103-107	D002285	denotes	DCIS
T113	103-107	D002285	denotes	DCIS
T114	131-134	Q41249	denotes	PCR
T115	131-134	O66148	denotes	PCR
T116	131-134	Q59987	denotes	PCR
T117	131-134	Q01289	denotes	PCR
T118	131-134	O80333	denotes	PCR
T119	131-134	P15904	denotes	PCR
T120	131-134	Q9SDT1	denotes	PCR
T121	131-134	Q39617	denotes	PCR
T123	201-206	SO:0002031	denotes	shRNA
T122	201-206	D034741	denotes	shRNA
T124	266-270	PR:000007171	denotes	EREG
T125	266-270	PR:O14944	denotes	EREG
T126	266-270	PR:Q61521	denotes	EREG
T127	266-270	PR:Q9Z0L5	denotes	EREG
T129	285-294	CVCL_5552	denotes	MCF10DCIS
T130	290-294	D002285	denotes	DCIS
T132	290-294	D002285	denotes	DCIS
T133	355-359	PR:000007171	denotes	EREG
T134	355-359	PR:O14944	denotes	EREG
T135	355-359	PR:Q61521	denotes	EREG
T136	355-359	PR:Q9Z0L5	denotes	EREG
T137	369-374	D009369	denotes	tumor
T138	369-374	D009369	denotes	tumor
T139	369-379	CL:0001063	denotes	tumor cell
T140	495-500	D009369	denotes	tumor
T141	495-500	D009369	denotes	tumor

Id	Subject	Object	Predicate	Lexical cue
T5	0-167	DRI_Background	denotes	EREG expression levels were assessed in both cell lines and human samples of ductal carcinoma in situ (DCIS) using quantitative RT-PCR, ELISA and immunohistochemistry.
T6	168-389	DRI_Background	denotes	Gene knock-down approaches using shRNA-based strategies were used to determine the requirement of EREG for growth of MCF10DCIS cells in vivo, and for identifying mechanisms through which EREG promotes tumor cell survival.
T7	390-516	DRI_Background	denotes	Experiments were performed using a combination of two-dimensional culture, three-dimensional culture and tumor growth in vivo.

Id	Subject	Object	Predicate	Lexical cue
T2	0-516	METHODS	denotes	EREG expression levels were assessed in both cell lines and human samples of ductal carcinoma in situ (DCIS) using quantitative RT-PCR, ELISA and immunohistochemistry. Gene knock-down approaches using shRNA-based strategies were used to determine the requirement of EREG for growth of MCF10DCIS cells in vivo, and for identifying mechanisms through which EREG promotes tumor cell survival. Experiments were performed using a combination of two-dimensional culture, three-dimensional culture and tumor growth in vivo.