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PubMed:26214737 JSONTXT

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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 89-148 DRI_Background denotes Tissue morphogenesis is orchestrated by cell shape changes.
T2 149-285 DRI_Background denotes Forces required to power these changes are generated by non-muscle myosin II (MyoII) motor proteins pulling filamentous actin (F-actin).
T3 286-465 DRI_Approach denotes Actomyosin networks undergo cycles of assembly and disassembly (pulses) to cause cell deformations alternating with steps of stabilization to result in irreversible shape changes.
T4 466-579 DRI_Background denotes Although this ratchet-like behaviour operates in a variety of contexts, the underlying mechanisms remain unclear.
T5 580-718 DRI_Approach denotes Here we investigate the role of MyoII regulation through the conserved Rho1-Rok pathway during Drosophila melanogaster germband extension.
T6 719-932 DRI_Approach denotes This morphogenetic process is powered by cell intercalation, which involves the shrinkage of junctions in the dorsal-ventral axis (vertical junctions) followed by junction extension in the anterior-posterior axis.
T7 933-957 DRI_Approach denotes While polarized flows of
T8 978-1082 DRI_Approach denotes pulses deform vertical junctions, MyoII enrichment on these junctions (planar polarity) stabilizes them.
T9 1083-1353 DRI_Approach denotes We identify two critical properties of MyoII dynamics that underlie stability and pulsatility: exchange kinetics governed by phosphorylation-dephosphorylation cycles of the MyoII regulatory light chain; and advection due to contraction of the motors on F-actin networks.
T10 1354-1501 DRI_Background denotes Spatial control over MyoII exchange kinetics establishes two stable regimes of high and low dissociation rates, resulting in MyoII planar polarity.
T11 1502-1661 DRI_Background denotes Pulsatility emerges at intermediate dissociation rates, enabling convergent advection of MyoII and its upstream regulators Rho1 GTP, Rok and MyoII phosphatase.
T12 1662-1731 DRI_Approach denotes Notably, pulsatility is not an outcome of an upstream Rho1 pacemaker.
T13 1732-1876 DRI_Background denotes Rather, it is a self-organized system that involves positive and negative biomechanical feedback between MyoII advection and dissociation rates.