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Characterization of free and conjugated phenolic compounds in fruits of selected wild plants.
A gas chromatography-mass spectrometric (GC-MS) method was utilized for the separation, and systematic characterization of phenolic compounds as trimethylsilyl derivatives in fruits of wild plants including Olive, Jujube and Common Fig. Both the free and conjugate phenolic acids (rarely determined before and several are reported first time here) were characterized. A baseline separation of the 20 phenolics was achieved in 25 min with standard calibration curves linear over the concentration range from the detection limits to 20 μg/mL. Total of fourteen phenolic acids were identified in wild Olive fruit, eight in wild Jujube fruit and ten in wild Common Fig fruit, out of which 2,4-dihydroxybenzoic acid and trans-cinnamic acid were dominant in these fruits with concentration of 87.02, 5.25 and 14.16 mg/kg and 32.43, 5.77 and 11.70 mg/kg (dry weight), respectively. The results of this study support the utilization of the tested wild fruits as a potential source of valuable phenolics for functional food and nutraceutical applications.
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