PubMed:25839646 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25839646","sourcedb":"PubMed","sourceid":"25839646","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/25839646","text":"Cyclosporine A prevents ex vivo PCO formation through induction of autophagy-mediated cell death.\nThe purpose of this study was to determine the Cyclosporine A (CsA) dose and minimum drug delivery time needed to prevent posterior capsule opacification (PCO) in an ex vivo canine model and evaluate the mechanism of CsA-induced cell death. Canine lens epithelial cells (LEC) were treated with CsA and changes in cell migration, proliferation, and density were monitored over time. CsA-treated LEC underwent transmission electron microscopy (TEM), immunofluorescence, and immunoblotting in the presence or absence of autophagy inhibitors to evaluate the mechanism of cell death. Lens capsules were harvested from canine cadaver eyes for an ex vivo model of PCO. Lens capsules were treated with CsA for 1, 2, 3, 4, 5, 6, or 7 days, and subsequently maintained in culture for a total of 28 days in the absence of drug. CsA reduced LEC viability in a dose dependent manner. Morphologically, CsA-treated LEC were swollen, had intact nuclei, lacked peripheral chromatin condensation, and demonstrated prominent vacuolization; TEM revealed autophagosomes. LC3-II protein expression and acridine orange fluorescence increased in CsA-treated cells. A small non-significant induction of cleaved caspase-3 was observed in CsA-treated LEC. Lens capsules treated with 5, 6, or 7 days of 10 μg/mL CsA showed a significant decrease in ex vivo PCO formation; 6 days of drug delivery prevented PCO. This study finds that morphologic changes, formation of acidic vesicles, and increased expression of LC3-II supports the hypothesis that CsA mediates LEC death via autophagy; this is a novel finding in the lens. Induction of CsA-induced apoptosis was minimal. Six days of intracapsular CsA drug delivery prevented ex vivo PCO formation.","tracks":[{"project":"GoldHamster","denotations":[{"id":"T3","span":{"begin":0,"end":12},"obj":"3008"},{"id":"T5","span":{"begin":0,"end":14},"obj":"D016572"},{"id":"T6","span":{"begin":0,"end":14},"obj":"D016572"},{"id":"T7","span":{"begin":32,"end":35},"obj":"D011085"},{"id":"T8","span":{"begin":36,"end":45},"obj":"GO:0009058"},{"id":"T9","span":{"begin":67,"end":76},"obj":"GO:0016236"},{"id":"T10","span":{"begin":67,"end":76},"obj":"GO:0006914"},{"id":"T11","span":{"begin":86,"end":96},"obj":"GO:0008219"},{"id":"T14","span":{"begin":145,"end":157},"obj":"3008"},{"id":"T16","span":{"begin":145,"end":159},"obj":"D016572"},{"id":"T17","span":{"begin":145,"end":159},"obj":"D016572"},{"id":"T19","span":{"begin":161,"end":164},"obj":"PR:Q8CFD5"},{"id":"T20","span":{"begin":161,"end":164},"obj":"PR:000007170"},{"id":"T22","span":{"begin":161,"end":164},"obj":"P08796"},{"id":"T23","span":{"begin":161,"end":164},"obj":"PR:Q13216"},{"id":"T21","span":{"begin":161,"end":164},"obj":"CHEBI:55379"},{"id":"T18","span":{"begin":161,"end":164},"obj":"D003057"},{"id":"T24","span":{"begin":183,"end":187},"obj":"CHEBI:23888"},{"id":"T25","span":{"begin":220,"end":229},"obj":"UBERON:0001353"},{"id":"T26","span":{"begin":230,"end":237},"obj":"GO:0042603"},{"id":"T27","span":{"begin":230,"end":237},"obj":"UBERON:0003893"},{"id":"T28","span":{"begin":230,"end":251},"obj":"D058442"},{"id":"T29","span":{"begin":230,"end":251},"obj":"D058442"},{"id":"T30","span":{"begin":253,"end":256},"obj":"D011085"},{"id":"T31","span":{"begin":272,"end":278},"obj":"UBERON:0003674"},{"id":"T33","span":{"begin":315,"end":318},"obj":"PR:Q8CFD5"},{"id":"T34","span":{"begin":315,"end":318},"obj":"PR:000007170"},{"id":"T36","span":{"begin":315,"end":318},"obj":"P08796"},{"id":"T37","span":{"begin":315,"end":318},"obj":"PR:Q13216"},{"id":"T35","span":{"begin":315,"end":318},"obj":"CHEBI:55379"},{"id":"T32","span":{"begin":315,"end":318},"obj":"D003057"},{"id":"T38","span":{"begin":327,"end":337},"obj":"GO:0008219"},{"id":"T39","span":{"begin":339,"end":345},"obj":"UBERON:0003674"},{"id":"T40","span":{"begin":346,"end":350},"obj":"UBERON:0000965"},{"id":"T41","span":{"begin":346,"end":350},"obj":"UBERON:0005389"},{"id":"T42","span":{"begin":369,"end":372},"obj":"CL:0002138"},{"id":"T44","span":{"begin":369,"end":372},"obj":"PR:Q9LJR2"},{"id":"T43","span":{"begin":369,"end":372},"obj":"UBERON:0007225"},{"id":"T46","span":{"begin":392,"end":395},"obj":"PR:Q8CFD5"},{"id":"T47","span":{"begin":392,"end":395},"obj":"PR:000007170"},{"id":"T49","span":{"begin":392,"end":395},"obj":"P08796"},{"id":"T50","span":{"begin":392,"end":395},"obj":"PR:Q13216"},{"id":"T48","span":{"begin":392,"end":395},"obj":"CHEBI:55379"},{"id":"T45","span":{"begin":392,"end":395},"obj":"D003057"},{"id":"T51","span":{"begin":411,"end":425},"obj":"GO:0016477"},{"id":"T52","span":{"begin":427,"end":440},"obj":"UBERON:2000098"},{"id":"T54","span":{"begin":480,"end":483},"obj":"PR:Q8CFD5"},{"id":"T55","span":{"begin":480,"end":483},"obj":"PR:000007170"},{"id":"T57","span":{"begin":480,"end":483},"obj":"P08796"},{"id":"T58","span":{"begin":480,"end":483},"obj":"PR:Q13216"},{"id":"T56","span":{"begin":480,"end":483},"obj":"CHEBI:55379"},{"id":"T53","span":{"begin":480,"end":483},"obj":"D003057"},{"id":"T59","span":{"begin":492,"end":495},"obj":"CL:0002138"},{"id":"T61","span":{"begin":492,"end":495},"obj":"PR:Q9LJR2"},{"id":"T60","span":{"begin":492,"end":495},"obj":"UBERON:0007225"},{"id":"T62","span":{"begin":519,"end":527},"obj":"CHEBI:10545"},{"id":"T66","span":{"begin":540,"end":543},"obj":"GO:0097197"},{"id":"T63","span":{"begin":540,"end":543},"obj":"PR:000016500"},{"id":"T64","span":{"begin":540,"end":543},"obj":"Q9H2S6"},{"id":"T67","span":{"begin":540,"end":543},"obj":"Q9EP64"},{"id":"T68","span":{"begin":540,"end":543},"obj":"Q9ESC2"},{"id":"T65","span":{"begin":540,"end":543},"obj":"CVCL_E844"},{"id":"T69","span":{"begin":540,"end":543},"obj":"CHEBI:27919"},{"id":"T70","span":{"begin":615,"end":624},"obj":"GO:0016236"},{"id":"T71","span":{"begin":615,"end":624},"obj":"GO:0006914"},{"id":"T72","span":{"begin":615,"end":635},"obj":"CHEBI:88230"},{"id":"T74","span":{"begin":665,"end":675},"obj":"GO:0008219"},{"id":"T75","span":{"begin":677,"end":681},"obj":"UBERON:0000965"},{"id":"T76","span":{"begin":677,"end":681},"obj":"UBERON:0005389"},{"id":"T77","span":{"begin":682,"end":690},"obj":"D002214"},{"id":"T78","span":{"begin":711,"end":717},"obj":"UBERON:0003674"},{"id":"T80","span":{"begin":718,"end":725},"obj":"UBERON:0008979"},{"id":"T79","span":{"begin":718,"end":725},"obj":"D002102"},{"id":"T81","span":{"begin":718,"end":725},"obj":"D002102"},{"id":"T82","span":{"begin":755,"end":758},"obj":"D011085"},{"id":"T83","span":{"begin":760,"end":764},"obj":"UBERON:0000965"},{"id":"T84","span":{"begin":760,"end":764},"obj":"UBERON:0005389"},{"id":"T85","span":{"begin":765,"end":773},"obj":"D002214"},{"id":"T87","span":{"begin":792,"end":795},"obj":"PR:Q8CFD5"},{"id":"T88","span":{"begin":792,"end":795},"obj":"PR:000007170"},{"id":"T90","span":{"begin":792,"end":795},"obj":"P08796"},{"id":"T91","span":{"begin":792,"end":795},"obj":"PR:Q13216"},{"id":"T89","span":{"begin":792,"end":795},"obj":"CHEBI:55379"},{"id":"T86","span":{"begin":792,"end":795},"obj":"D003057"},{"id":"T93","span":{"begin":909,"end":913},"obj":"CHEBI:23888"},{"id":"T95","span":{"begin":915,"end":918},"obj":"PR:Q8CFD5"},{"id":"T96","span":{"begin":915,"end":918},"obj":"PR:000007170"},{"id":"T98","span":{"begin":915,"end":918},"obj":"P08796"},{"id":"T99","span":{"begin":915,"end":918},"obj":"PR:Q13216"},{"id":"T97","span":{"begin":915,"end":918},"obj":"CHEBI:55379"},{"id":"T94","span":{"begin":915,"end":918},"obj":"D003057"},{"id":"T102","span":{"begin":927,"end":930},"obj":"PR:Q9LJR2"},{"id":"T101","span":{"begin":927,"end":930},"obj":"UBERON:0007225"},{"id":"T100","span":{"begin":927,"end":930},"obj":"CL:0002138"},{"id":"T104","span":{"begin":986,"end":989},"obj":"PR:Q8CFD5"},{"id":"T105","span":{"begin":986,"end":989},"obj":"PR:000007170"},{"id":"T107","span":{"begin":986,"end":989},"obj":"P08796"},{"id":"T108","span":{"begin":986,"end":989},"obj":"PR:Q13216"},{"id":"T106","span":{"begin":986,"end":989},"obj":"CHEBI:55379"},{"id":"T103","span":{"begin":986,"end":989},"obj":"D003057"},{"id":"T109","span":{"begin":998,"end":1001},"obj":"CL:0002138"},{"id":"T111","span":{"begin":998,"end":1001},"obj":"PR:Q9LJR2"},{"id":"T110","span":{"begin":998,"end":1001},"obj":"UBERON:0007225"},{"id":"T112","span":{"begin":1027,"end":1033},"obj":"CHEBI:33252"},{"id":"T114","span":{"begin":1053,"end":1062},"obj":"GO:0000785"},{"id":"T113","span":{"begin":1053,"end":1062},"obj":"D002843"},{"id":"T117","span":{"begin":1119,"end":1122},"obj":"CVCL_E844"},{"id":"T115","span":{"begin":1119,"end":1122},"obj":"PR:000016500"},{"id":"T116","span":{"begin":1119,"end":1122},"obj":"Q9H2S6"},{"id":"T119","span":{"begin":1119,"end":1122},"obj":"Q9EP64"},{"id":"T120","span":{"begin":1119,"end":1122},"obj":"Q9ESC2"},{"id":"T118","span":{"begin":1119,"end":1122},"obj":"GO:0097197"},{"id":"T121","span":{"begin":1119,"end":1122},"obj":"CHEBI:27919"},{"id":"T122","span":{"begin":1148,"end":1151},"obj":"P82160"},{"id":"T127","span":{"begin":1155,"end":1162},"obj":"SO:0000104"},{"id":"T126","span":{"begin":1155,"end":1162},"obj":"PR:000000001"},{"id":"T123","span":{"begin":1155,"end":1162},"obj":"GO:0003675"},{"id":"T124","span":{"begin":1155,"end":1162},"obj":"CHEBI:36080"},{"id":"T125","span":{"begin":1155,"end":1162},"obj":"CHEBI:11122"},{"id":"T129","span":{"begin":1178,"end":1193},"obj":"D000165"},{"id":"T130","span":{"begin":1178,"end":1193},"obj":"D000165"},{"id":"T131","span":{"begin":1178,"end":1193},"obj":"CHEBI:87346"},{"id":"T132","span":{"begin":1178,"end":1193},"obj":"CHEBI:51739"},{"id":"T133","span":{"begin":1187,"end":1193},"obj":"127424"},{"id":"T135","span":{"begin":1220,"end":1223},"obj":"PR:Q8CFD5"},{"id":"T136","span":{"begin":1220,"end":1223},"obj":"PR:000007170"},{"id":"T138","span":{"begin":1220,"end":1223},"obj":"P08796"},{"id":"T139","span":{"begin":1220,"end":1223},"obj":"PR:Q13216"},{"id":"T137","span":{"begin":1220,"end":1223},"obj":"CHEBI:55379"},{"id":"T134","span":{"begin":1220,"end":1223},"obj":"D003057"},{"id":"T142","span":{"begin":1284,"end":1293},"obj":"P55866"},{"id":"T143","span":{"begin":1284,"end":1293},"obj":"Q8MJC3"},{"id":"T144","span":{"begin":1284,"end":1293},"obj":"Q2PFV2"},{"id":"T145","span":{"begin":1284,"end":1293},"obj":"Q08DY9"},{"id":"T146","span":{"begin":1284,"end":1293},"obj":"Q5IS54"},{"id":"T147","span":{"begin":1284,"end":1293},"obj":"Q8MKI5"},{"id":"T148","span":{"begin":1284,"end":1293},"obj":"PR:000002312"},{"id":"T149","span":{"begin":1284,"end":1293},"obj":"Q8MJU1"},{"id":"T150","span":{"begin":1284,"end":1293},"obj":"Q60431"},{"id":"T151","span":{"begin":1284,"end":1293},"obj":"P42574"},{"id":"T152","span":{"begin":1284,"end":1293},"obj":"Q5IS99"},{"id":"T153","span":{"begin":1284,"end":1293},"obj":"Q95ND5"},{"id":"T154","span":{"begin":1284,"end":1293},"obj":"P70677"},{"id":"T155","span":{"begin":1284,"end":1293},"obj":"P55213"},{"id":"T141","span":{"begin":1284,"end":1293},"obj":"D053148"},{"id":"T157","span":{"begin":1310,"end":1313},"obj":"PR:Q8CFD5"},{"id":"T158","span":{"begin":1310,"end":1313},"obj":"PR:000007170"},{"id":"T160","span":{"begin":1310,"end":1313},"obj":"P08796"},{"id":"T161","span":{"begin":1310,"end":1313},"obj":"PR:Q13216"},{"id":"T159","span":{"begin":1310,"end":1313},"obj":"CHEBI:55379"},{"id":"T156","span":{"begin":1310,"end":1313},"obj":"D003057"},{"id":"T164","span":{"begin":1322,"end":1325},"obj":"PR:Q9LJR2"},{"id":"T163","span":{"begin":1322,"end":1325},"obj":"UBERON:0007225"},{"id":"T162","span":{"begin":1322,"end":1325},"obj":"CL:0002138"},{"id":"T165","span":{"begin":1327,"end":1331},"obj":"UBERON:0000965"},{"id":"T166","span":{"begin":1327,"end":1331},"obj":"UBERON:0005389"},{"id":"T167","span":{"begin":1332,"end":1340},"obj":"D002214"},{"id":"T174","span":{"begin":1382,"end":1385},"obj":"PR:Q8CFD5"},{"id":"T175","span":{"begin":1382,"end":1385},"obj":"PR:000007170"},{"id":"T177","span":{"begin":1382,"end":1385},"obj":"P08796"},{"id":"T178","span":{"begin":1382,"end":1385},"obj":"PR:Q13216"},{"id":"T176","span":{"begin":1382,"end":1385},"obj":"CHEBI:55379"},{"id":"T173","span":{"begin":1382,"end":1385},"obj":"D003057"},{"id":"T179","span":{"begin":1427,"end":1430},"obj":"D011085"},{"id":"T180","span":{"begin":1431,"end":1440},"obj":"GO:0009058"},{"id":"T181","span":{"begin":1452,"end":1456},"obj":"CHEBI:23888"},{"id":"T182","span":{"begin":1476,"end":1479},"obj":"D011085"},{"id":"T183","span":{"begin":1524,"end":1533},"obj":"GO:0009058"},{"id":"T184","span":{"begin":1582,"end":1585},"obj":"P82160"},{"id":"T186","span":{"begin":1618,"end":1621},"obj":"PR:Q8CFD5"},{"id":"T187","span":{"begin":1618,"end":1621},"obj":"PR:000007170"},{"id":"T189","span":{"begin":1618,"end":1621},"obj":"P08796"},{"id":"T190","span":{"begin":1618,"end":1621},"obj":"PR:Q13216"},{"id":"T188","span":{"begin":1618,"end":1621},"obj":"CHEBI:55379"},{"id":"T185","span":{"begin":1618,"end":1621},"obj":"D003057"},{"id":"T191","span":{"begin":1631,"end":1634},"obj":"CL:0002138"},{"id":"T193","span":{"begin":1631,"end":1634},"obj":"PR:Q9LJR2"},{"id":"T192","span":{"begin":1631,"end":1634},"obj":"UBERON:0007225"},{"id":"T194","span":{"begin":1645,"end":1654},"obj":"GO:0016236"},{"id":"T195","span":{"begin":1645,"end":1654},"obj":"GO:0006914"},{"id":"T196","span":{"begin":1687,"end":1691},"obj":"UBERON:0000965"},{"id":"T197","span":{"begin":1687,"end":1691},"obj":"UBERON:0005389"},{"id":"T199","span":{"begin":1706,"end":1709},"obj":"PR:Q8CFD5"},{"id":"T200","span":{"begin":1706,"end":1709},"obj":"PR:000007170"},{"id":"T202","span":{"begin":1706,"end":1709},"obj":"P08796"},{"id":"T203","span":{"begin":1706,"end":1709},"obj":"PR:Q13216"},{"id":"T201","span":{"begin":1706,"end":1709},"obj":"CHEBI:55379"},{"id":"T198","span":{"begin":1706,"end":1709},"obj":"D003057"},{"id":"T204","span":{"begin":1718,"end":1727},"obj":"GO:0097194"},{"id":"T205","span":{"begin":1718,"end":1727},"obj":"GO:0006915"},{"id":"T207","span":{"begin":1767,"end":1770},"obj":"PR:Q8CFD5"},{"id":"T208","span":{"begin":1767,"end":1770},"obj":"PR:000007170"},{"id":"T210","span":{"begin":1767,"end":1770},"obj":"P08796"},{"id":"T211","span":{"begin":1767,"end":1770},"obj":"PR:Q13216"},{"id":"T209","span":{"begin":1767,"end":1770},"obj":"CHEBI:55379"},{"id":"T206","span":{"begin":1767,"end":1770},"obj":"D003057"},{"id":"T212","span":{"begin":1771,"end":1775},"obj":"CHEBI:23888"},{"id":"T213","span":{"begin":6,"end":9},"obj":"D011085"},{"id":"T214","span":{"begin":10,"end":19},"obj":"GO:0009058"}],"attributes":[{"subj":"T3","pred":"source","obj":"GoldHamster"},{"subj":"T5","pred":"source","obj":"GoldHamster"},{"subj":"T6","pred":"source","obj":"GoldHamster"},{"subj":"T7","pred":"source","obj":"GoldHamster"},{"subj":"T8","pred":"source","obj":"GoldHamster"},{"subj":"T9","pred":"source","obj":"GoldHamster"},{"subj":"T10","pred":"source","obj":"GoldHamster"},{"subj":"T11","pred":"source","obj":"GoldHamster"},{"subj":"T14","pred":"source","obj":"GoldHamster"},{"subj":"T16","pred":"source","obj":"GoldHamster"},{"subj":"T17","pred":"source","obj":"GoldHamster"},{"subj":"T19","pred":"source","obj":"GoldHamster"},{"subj":"T20","pred":"source","obj":"GoldHamster"},{"subj":"T22","pred":"source","obj":"GoldHamster"},{"subj":"T23","pred":"source","obj":"GoldHamster"},{"subj":"T21","pred":"source","obj":"GoldHamster"},{"subj":"T18","pred":"source","obj":"GoldHamster"},{"subj":"T24","pred":"source","obj":"GoldHamster"},{"subj":"T25","pred":"source","obj":"GoldHamster"},{"subj":"T26","pred":"source","obj":"GoldHamster"},{"subj":"T27","pred":"source","obj":"GoldHamster"},{"subj":"T28","pred":"source","obj":"GoldHamster"},{"subj":"T29","pred":"source","obj":"GoldHamster"},{"subj":"T30","pred":"source","obj":"GoldHamster"},{"subj":"T31","pred":"source","obj":"GoldHamster"},{"subj":"T33","pred":"source","obj":"GoldHamster"},{"subj":"T34","pred":"source","obj":"GoldHamster"},{"subj":"T36","pred":"source","obj":"GoldHamster"},{"subj":"T37","pred":"source","obj":"GoldHamster"},{"subj":"T35","pred":"source","obj":"GoldHamster"},{"subj":"T32","pred":"source","obj":"GoldHamster"},{"subj":"T38","pred":"source","obj":"GoldHamster"},{"subj":"T39","pred":"source","obj":"GoldHamster"},{"subj":"T40","pred":"source","obj":"GoldHamster"},{"subj":"T41","pred":"source","obj":"GoldHamster"},{"subj":"T42","pred":"source","obj":"GoldHamster"},{"subj":"T44","pred":"source","obj":"GoldHamster"},{"subj":"T43","pred":"source","obj":"GoldHamster"},{"subj":"T46","pred":"source","obj":"GoldHamster"},{"subj":"T47","pred":"source","obj":"GoldHamster"},{"subj":"T49","pred":"source","obj":"GoldHamster"},{"subj":"T50","pred":"source","obj":"GoldHamster"},{"subj":"T48","pred":"source","obj":"GoldHamster"},{"subj":"T45","pred":"source","obj":"GoldHamster"},{"subj":"T51","pred":"source","obj":"GoldHamster"},{"subj":"T52","pred":"source","obj":"GoldHamster"},{"subj":"T54","pred":"source","obj":"GoldHamster"},{"subj":"T55","pred":"source","obj":"GoldHamster"},{"subj":"T57","pred":"source","obj":"GoldHamster"},{"subj":"T58","pred":"source","obj":"GoldHamster"},{"subj":"T56","pred":"source","obj":"GoldHamster"},{"subj":"T53","pred":"source","obj":"GoldHamster"},{"subj":"T59","pred":"source","obj":"GoldHamster"},{"subj":"T61","pred":"source","obj":"GoldHamster"},{"subj":"T60","pred":"source","obj":"GoldHamster"},{"subj":"T62","pred":"source","obj":"GoldHamster"},{"subj":"T66","pred":"source","obj":"GoldHamster"},{"subj":"T63","pred":"source","obj":"GoldHamster"},{"subj":"T64","pred":"source","obj":"GoldHamster"},{"subj":"T67","pred":"source","obj":"GoldHamster"},{"subj":"T68","pred":"source","obj":"GoldHamster"},{"subj":"T65","pred":"source","obj":"GoldHamster"},{"subj":"T69","pred":"source","obj":"GoldHamster"},{"subj":"T70","pred":"source","obj":"GoldHamster"},{"subj":"T71","pred":"source","obj":"GoldHamster"},{"subj":"T72","pred":"source","obj":"GoldHamster"},{"subj":"T74","pred":"source","obj":"GoldHamster"},{"subj":"T75","pred":"source","obj":"GoldHamster"},{"subj":"T76","pred":"source","obj":"GoldHamster"},{"subj":"T77","pred":"source","obj":"GoldHamster"},{"subj":"T78","pred":"source","obj":"GoldHamster"},{"subj":"T80","pred":"source","obj":"GoldHamster"},{"subj":"T79","pred":"source","obj":"GoldHamster"},{"subj":"T81","pred":"source","obj":"GoldHamster"},{"subj":"T82","pred":"source","obj":"GoldHamster"},{"subj":"T83","pred":"source","obj":"GoldHamster"},{"subj":"T84","pred":"source","obj":"GoldHamster"},{"subj":"T85","pred":"source","obj":"GoldHamster"},{"subj":"T87","pred":"source","obj":"GoldHamster"},{"subj":"T88","pred":"source","obj":"GoldHamster"},{"subj":"T90","pred":"source","obj":"GoldHamster"},{"subj":"T91","pred":"source","obj":"GoldHamster"},{"subj":"T89","pred":"source","obj":"GoldHamster"},{"subj":"T86","pred":"source","obj":"GoldHamster"},{"subj":"T93","pred":"source","obj":"GoldHamster"},{"subj":"T95","pred":"source","obj":"GoldHamster"},{"subj":"T96","pred":"source","obj":"GoldHamster"},{"subj":"T98","pred":"source","obj":"GoldHamster"},{"subj":"T99","pred":"source","obj":"GoldHamster"},{"subj":"T97","pred":"source","obj":"GoldHamster"},{"subj":"T94","pred":"source","obj":"GoldHamster"},{"subj":"T102","pred":"source","obj":"GoldHamster"},{"subj":"T101","pred":"source","obj":"GoldHamster"},{"subj":"T100","pred":"source","obj":"GoldHamster"},{"subj":"T104","pred":"source","obj":"GoldHamster"},{"subj":"T105","pred":"source","obj":"GoldHamster"},{"subj":"T107","pred":"source","obj":"GoldHamster"},{"subj":"T108","pred":"source","obj":"GoldHamster"},{"subj":"T106","pred":"source","obj":"GoldHamster"},{"subj":"T103","pred":"source","obj":"GoldHamster"},{"subj":"T109","pred":"source","obj":"GoldHamster"},{"subj":"T111","pred":"source","obj":"GoldHamster"},{"subj":"T110","pred":"source","obj":"GoldHamster"},{"subj":"T112","pred":"source","obj":"GoldHamster"},{"subj":"T114","pred":"source","obj":"GoldHamster"},{"subj":"T113","pred":"source","obj":"GoldHamster"},{"subj":"T117","pred":"source","obj":"GoldHamster"},{"subj":"T115","pred":"source","obj":"GoldHamster"},{"subj":"T116","pred":"source","obj":"GoldHamster"},{"subj":"T119","pred":"source","obj":"GoldHamster"},{"subj":"T120","pred":"source","obj":"GoldHamster"},{"subj":"T118","pred":"source","obj":"GoldHamster"},{"subj":"T121","pred":"source","obj":"GoldHamster"},{"subj":"T122","pred":"source","obj":"GoldHamster"},{"subj":"T127","pred":"source","obj":"GoldHamster"},{"subj":"T126","pred":"source","obj":"GoldHamster"},{"subj":"T123","pred":"source","obj":"GoldHamster"},{"subj":"T124","pred":"source","obj":"GoldHamster"},{"subj":"T125","pred":"source","obj":"GoldHamster"},{"subj":"T129","pred":"source","obj":"GoldHamster"},{"subj":"T130","pred":"source","obj":"GoldHamster"},{"subj":"T131","pred":"source","obj":"GoldHamster"},{"subj":"T132","pred":"source","obj":"GoldHamster"},{"subj":"T133","pred":"source","obj":"GoldHamster"},{"subj":"T135","pred":"source","obj":"GoldHamster"},{"subj":"T136","pred":"source","obj":"GoldHamster"},{"subj":"T138","pred":"source","obj":"GoldHamster"},{"subj":"T139","pred":"source","obj":"GoldHamster"},{"subj":"T137","pred":"source","obj":"GoldHamster"},{"subj":"T134","pred":"source","obj":"GoldHamster"},{"subj":"T142","pred":"source","obj":"GoldHamster"},{"subj":"T143","pred":"source","obj":"GoldHamster"},{"subj":"T144","pred":"source","obj":"GoldHamster"},{"subj":"T145","pred":"source","obj":"GoldHamster"},{"subj":"T146","pred":"source","obj":"GoldHamster"},{"subj":"T147","pred":"source","obj":"GoldHamster"},{"subj":"T148","pred":"source","obj":"GoldHamster"},{"subj":"T149","pred":"source","obj":"GoldHamster"},{"subj":"T150","pred":"source","obj":"GoldHamster"},{"subj":"T151","pred":"source","obj":"GoldHamster"},{"subj":"T152","pred":"source","obj":"GoldHamster"},{"subj":"T153","pred":"source","obj":"GoldHamster"},{"subj":"T154","pred":"source","obj":"GoldHamster"},{"subj":"T155","pred":"source","obj":"GoldHamster"},{"subj":"T141","pred":"source","obj":"GoldHamster"},{"subj":"T157","pred":"source","obj":"GoldHamster"},{"subj":"T158","pred":"source","obj":"GoldHamster"},{"subj":"T160","pred":"source","obj":"GoldHamster"},{"subj":"T161","pred":"source","obj":"GoldHamster"},{"subj":"T159","pred":"source","obj":"GoldHamster"},{"subj":"T156","pred":"source","obj":"GoldHamster"},{"subj":"T164","pred":"source","obj":"GoldHamster"},{"subj":"T163","pred":"source","obj":"GoldHamster"},{"subj":"T162","pred":"source","obj":"GoldHamster"},{"subj":"T165","pred":"source","obj":"GoldHamster"},{"subj":"T166","pred":"source","obj":"GoldHamster"},{"subj":"T167","pred":"source","obj":"GoldHamster"},{"subj":"T174","pred":"source","obj":"GoldHamster"},{"subj":"T175","pred":"source","obj":"GoldHamster"},{"subj":"T177","pred":"source","obj":"GoldHamster"},{"subj":"T178","pred":"source","obj":"GoldHamster"},{"subj":"T176","pred":"source","obj":"GoldHamster"},{"subj":"T173","pred":"source","obj":"GoldHamster"},{"subj":"T179","pred":"source","obj":"GoldHamster"},{"subj":"T180","pred":"source","obj":"GoldHamster"},{"subj":"T181","pred":"source","obj":"GoldHamster"},{"subj":"T182","pred":"source","obj":"GoldHamster"},{"subj":"T183","pred":"source","obj":"GoldHamster"},{"subj":"T184","pred":"source","obj":"GoldHamster"},{"subj":"T186","pred":"source","obj":"GoldHamster"},{"subj":"T187","pred":"source","obj":"GoldHamster"},{"subj":"T189","pred":"source","obj":"GoldHamster"},{"subj":"T190","pred":"source","obj":"GoldHamster"},{"subj":"T188","pred":"source","obj":"GoldHamster"},{"subj":"T185","pred":"source","obj":"GoldHamster"},{"subj":"T191","pred":"source","obj":"GoldHamster"},{"subj":"T193","pred":"source","obj":"GoldHamster"},{"subj":"T192","pred":"source","obj":"GoldHamster"},{"subj":"T194","pred":"source","obj":"GoldHamster"},{"subj":"T195","pred":"source","obj":"GoldHamster"},{"subj":"T196","pred":"source","obj":"GoldHamster"},{"subj":"T197","pred":"source","obj":"GoldHamster"},{"subj":"T199","pred":"source","obj":"GoldHamster"},{"subj":"T200","pred":"source","obj":"GoldHamster"},{"subj":"T202","pred":"source","obj":"GoldHamster"},{"subj":"T203","pred":"source","obj":"GoldHamster"},{"subj":"T201","pred":"source","obj":"GoldHamster"},{"subj":"T198","pred":"source","obj":"GoldHamster"},{"subj":"T204","pred":"source","obj":"GoldHamster"},{"subj":"T205","pred":"source","obj":"GoldHamster"},{"subj":"T207","pred":"source","obj":"GoldHamster"},{"subj":"T208","pred":"source","obj":"GoldHamster"},{"subj":"T210","pred":"source","obj":"GoldHamster"},{"subj":"T211","pred":"source","obj":"GoldHamster"},{"subj":"T209","pred":"source","obj":"GoldHamster"},{"subj":"T206","pred":"source","obj":"GoldHamster"},{"subj":"T212","pred":"source","obj":"GoldHamster"},{"subj":"T213","pred":"source","obj":"GoldHamster"},{"subj":"T214","pred":"source","obj":"GoldHamster"}]},{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":98,"end":338},"obj":"DRI_Approach"},{"id":"T2","span":{"begin":339,"end":479},"obj":"DRI_Background"},{"id":"T3","span":{"begin":480,"end":676},"obj":"DRI_Background"},{"id":"T4","span":{"begin":677,"end":759},"obj":"DRI_Outcome"},{"id":"T5","span":{"begin":760,"end":914},"obj":"DRI_Approach"},{"id":"T6","span":{"begin":915,"end":968},"obj":"DRI_Approach"},{"id":"T7","span":{"begin":969,"end":1147},"obj":"DRI_Outcome"},{"id":"T8","span":{"begin":1148,"end":1238},"obj":"DRI_Background"},{"id":"T9","span":{"begin":1239,"end":1326},"obj":"DRI_Background"},{"id":"T10","span":{"begin":1481,"end":1692},"obj":"DRI_Challenge"},{"id":"T11","span":{"begin":1693,"end":1740},"obj":"DRI_Background"},{"id":"T12","span":{"begin":1741,"end":1817},"obj":"DRI_Approach"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T2","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T3","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T4","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T5","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T6","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T7","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T8","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T9","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T10","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T11","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T12","pred":"source","obj":"PubMed_ArguminSci"}]},{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T1","span":{"begin":13,"end":14},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T2","span":{"begin":158,"end":159},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T3","span":{"begin":197,"end":201},"obj":"CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens"},{"id":"T4","span":{"begin":261,"end":263},"obj":"CVCL_8754|Cancer_cell_line|Homo sapiens"},{"id":"T5","span":{"begin":261,"end":263},"obj":"CVCL_H241|Cancer_cell_line|Homo sapiens"},{"id":"T6","span":{"begin":400,"end":407},"obj":"CVCL_0238|Cancer_cell_line|Homo sapiens"},{"id":"T7","span":{"begin":474,"end":478},"obj":"CVCL_0047|Telomerase_immortalized_cell_line|Homo sapiens"},{"id":"T8","span":{"begin":540,"end":543},"obj":"CVCL_E844|Transformed_cell_line|Homo sapiens"},{"id":"T9","span":{"begin":735,"end":737},"obj":"CVCL_8754|Cancer_cell_line|Homo sapiens"},{"id":"T10","span":{"begin":735,"end":737},"obj":"CVCL_H241|Cancer_cell_line|Homo sapiens"},{"id":"T11","span":{"begin":800,"end":807},"obj":"CVCL_HE67|Factor-dependent_cell_line|Mus musculus"},{"id":"T12","span":{"begin":809,"end":813},"obj":"CVCL_E420|Transformed_cell_line|Homo sapiens"},{"id":"T13","span":{"begin":815,"end":820},"obj":"CVCL_VN30|Cancer_cell_line|Homo sapiens"},{"id":"T14","span":{"begin":872,"end":873},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T15","span":{"begin":944,"end":945},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T16","span":{"begin":1119,"end":1122},"obj":"CVCL_E844|Transformed_cell_line|Homo sapiens"},{"id":"T17","span":{"begin":1239,"end":1240},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T18","span":{"begin":1357,"end":1362},"obj":"CVCL_VN30|Cancer_cell_line|Homo sapiens"},{"id":"T19","span":{"begin":1379,"end":1381},"obj":"CVCL_F566|Transformed_cell_line|Homo sapiens"},{"id":"T20","span":{"begin":1379,"end":1381},"obj":"CVCL_W799|Cancer_cell_line|Homo sapiens"},{"id":"T21","span":{"begin":1393,"end":1394},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T22","span":{"begin":1515,"end":1522},"obj":"CVCL_0238|Cancer_cell_line|Homo sapiens"},{"id":"T23","span":{"begin":1664,"end":1665},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"}],"attributes":[{"subj":"T1","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T2","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T3","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T4","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T5","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T6","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T7","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T8","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T9","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T10","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T11","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T12","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T13","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T14","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T15","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T16","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T17","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T18","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T19","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T20","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T21","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T22","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T23","pred":"source","obj":"Goldhamster2_Cellosaurus"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":1672,"end":1691},"obj":"HP_0000517"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GoldHamster","color":"#93e3ec","default":true},{"id":"PubMed_ArguminSci","color":"#ecda93"},{"id":"Goldhamster2_Cellosaurus","color":"#c093ec"},{"id":"PubmedHPO","color":"#93eca6"}]}]}}