PubMed:25677303 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25677303","sourcedb":"PubMed","sourceid":"25677303","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/25677303","text":"A new sequencing approach for N-unsubstituted heparin/heparan sulfate oligosaccharides.\nThe rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles. Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS. However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods. In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides. This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS). We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues. The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide. This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations. This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.","tracks":[{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T2","span":{"begin":46,"end":53},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T3","span":{"begin":339,"end":346},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T4","span":{"begin":339,"end":346},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T5","span":{"begin":743,"end":750},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T6","span":{"begin":743,"end":750},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycan-Motif"},{"subj":"T2","pred":"source","obj":"Glycan-Motif"},{"subj":"T3","pred":"source","obj":"Glycan-Motif"},{"subj":"T4","pred":"source","obj":"Glycan-Motif"},{"subj":"T5","pred":"source","obj":"Glycan-Motif"},{"subj":"T6","pred":"source","obj":"Glycan-Motif"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":339,"end":346},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":743,"end":750},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":54,"end":69},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T2","span":{"begin":151,"end":166},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T3","span":{"begin":168,"end":170},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T4","span":{"begin":347,"end":349},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T5","span":{"begin":436,"end":438},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T6","span":{"begin":751,"end":753},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"T7","span":{"begin":1753,"end":1755},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T2","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T3","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T4","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T5","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T6","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T7","pred":"source","obj":"Glycosmos6-GlycoEpitope"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":87},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":88,"end":227},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":228,"end":439},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":440,"end":625},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":626,"end":771},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":772,"end":966},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":967,"end":1277},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1278,"end":1419},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1420,"end":1649},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1650,"end":1891},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":87},"obj":"Sentence"},{"id":"T2","span":{"begin":88,"end":227},"obj":"Sentence"},{"id":"T3","span":{"begin":228,"end":439},"obj":"Sentence"},{"id":"T4","span":{"begin":440,"end":625},"obj":"Sentence"},{"id":"T5","span":{"begin":626,"end":771},"obj":"Sentence"},{"id":"T6","span":{"begin":772,"end":966},"obj":"Sentence"},{"id":"T7","span":{"begin":967,"end":1277},"obj":"Sentence"},{"id":"T8","span":{"begin":1278,"end":1419},"obj":"Sentence"},{"id":"T9","span":{"begin":1420,"end":1649},"obj":"Sentence"},{"id":"T10","span":{"begin":1650,"end":1891},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":87},"obj":"Sentence"},{"id":"T2","span":{"begin":88,"end":227},"obj":"Sentence"},{"id":"T3","span":{"begin":228,"end":439},"obj":"Sentence"},{"id":"T4","span":{"begin":440,"end":625},"obj":"Sentence"},{"id":"T5","span":{"begin":626,"end":771},"obj":"Sentence"},{"id":"T6","span":{"begin":772,"end":966},"obj":"Sentence"},{"id":"T7","span":{"begin":967,"end":1277},"obj":"Sentence"},{"id":"T8","span":{"begin":1278,"end":1419},"obj":"Sentence"},{"id":"T9","span":{"begin":1420,"end":1649},"obj":"Sentence"},{"id":"T10","span":{"begin":1650,"end":1891},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T2","span":{"begin":46,"end":53},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T3","span":{"begin":339,"end":346},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T4","span":{"begin":339,"end":346},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"},{"id":"T5","span":{"begin":743,"end":750},"obj":"https://glytoucan.org/Structures/Glycans/G00021MO"},{"id":"T6","span":{"begin":743,"end":750},"obj":"https://glytoucan.org/Structures/Glycans/G54161DR"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlycoBiology-GDGDB","denotations":[{"id":"_T1","span":{"begin":1012,"end":1014},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00386"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-GDGDB"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":46,"end":53},"obj":"FMAID:167420"},{"id":"_T2","span":{"begin":46,"end":53},"obj":"FMAID:82839"},{"id":"_T3","span":{"begin":54,"end":61},"obj":"FMAID:67110"},{"id":"_T4","span":{"begin":54,"end":61},"obj":"FMAID:165191"},{"id":"_T5","span":{"begin":54,"end":69},"obj":"FMAID:63023"},{"id":"_T6","span":{"begin":54,"end":69},"obj":"FMAID:167405"},{"id":"_T7","span":{"begin":70,"end":86},"obj":"FMAID:196731"},{"id":"_T8","span":{"begin":70,"end":86},"obj":"FMAID:82742"},{"id":"_T9","span":{"begin":113,"end":124},"obj":"FMAID:196792"},{"id":"_T10","span":{"begin":113,"end":124},"obj":"FMAID:82797"},{"id":"_T11","span":{"begin":151,"end":158},"obj":"FMAID:165191"},{"id":"_T12","span":{"begin":151,"end":158},"obj":"FMAID:67110"},{"id":"_T13","span":{"begin":151,"end":166},"obj":"FMAID:63023"},{"id":"_T14","span":{"begin":151,"end":166},"obj":"FMAID:167405"},{"id":"_T15","span":{"begin":339,"end":346},"obj":"FMAID:82839"},{"id":"_T16","span":{"begin":339,"end":346},"obj":"FMAID:167420"},{"id":"_T17","span":{"begin":350,"end":366},"obj":"FMAID:196731"},{"id":"_T18","span":{"begin":350,"end":366},"obj":"FMAID:82742"},{"id":"_T19","span":{"begin":500,"end":516},"obj":"FMAID:196731"},{"id":"_T20","span":{"begin":500,"end":516},"obj":"FMAID:82742"},{"id":"_T21","span":{"begin":670,"end":680},"obj":"FMAID:62083"},{"id":"_T22","span":{"begin":670,"end":680},"obj":"FMAID:165655"},{"id":"_T23","span":{"begin":743,"end":750},"obj":"FMAID:167420"},{"id":"_T24","span":{"begin":743,"end":750},"obj":"FMAID:82839"},{"id":"_T25","span":{"begin":754,"end":770},"obj":"FMAID:82742"},{"id":"_T26","span":{"begin":754,"end":770},"obj":"FMAID:196731"},{"id":"_T27","span":{"begin":883,"end":889},"obj":"FMAID:85815"},{"id":"_T28","span":{"begin":1056,"end":1058},"obj":"FMAID:63497"},{"id":"_T29","span":{"begin":1056,"end":1058},"obj":"FMAID:167722"},{"id":"_T30","span":{"begin":1059,"end":1061},"obj":"FMAID:167723"},{"id":"_T31","span":{"begin":1059,"end":1061},"obj":"FMAID:63498"},{"id":"_T32","span":{"begin":1074,"end":1090},"obj":"FMAID:196731"},{"id":"_T33","span":{"begin":1074,"end":1090},"obj":"FMAID:82742"},{"id":"_T34","span":{"begin":1181,"end":1197},"obj":"FMAID:82742"},{"id":"_T35","span":{"begin":1181,"end":1197},"obj":"FMAID:196731"},{"id":"_T36","span":{"begin":1403,"end":1418},"obj":"FMAID:196731"},{"id":"_T37","span":{"begin":1403,"end":1418},"obj":"FMAID:82742"},{"id":"_T38","span":{"begin":1596,"end":1611},"obj":"FMAID:196731"},{"id":"_T39","span":{"begin":1596,"end":1611},"obj":"FMAID:82742"},{"id":"_T40","span":{"begin":1731,"end":1747},"obj":"FMAID:196731"},{"id":"_T41","span":{"begin":1731,"end":1747},"obj":"FMAID:82742"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T15","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T16","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T17","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T18","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T19","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T20","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T21","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T22","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T23","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T24","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T25","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T26","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T27","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T28","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T29","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T30","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T31","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T32","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T33","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T34","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T35","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T36","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T37","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T38","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T39","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T40","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T41","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":909,"end":913},"obj":"http://www.uniprot.org/uniprot/Q9UFF0"},{"id":"T2","span":{"begin":962,"end":964},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T3","span":{"begin":1296,"end":1298},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T4","span":{"begin":1059,"end":1061},"obj":"http://www.uniprot.org/uniprot/P01031"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"},{"subj":"T2","pred":"source","obj":"uniprot-human"},{"subj":"T3","pred":"source","obj":"uniprot-human"},{"subj":"T4","pred":"source","obj":"uniprot-human"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":909,"end":913},"obj":"http://www.uniprot.org/uniprot/Q05117"},{"id":"T2","span":{"begin":909,"end":913},"obj":"http://www.uniprot.org/uniprot/Q8K1H1"},{"id":"T3","span":{"begin":962,"end":964},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T4","span":{"begin":1296,"end":1298},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T5","span":{"begin":1056,"end":1058},"obj":"http://www.uniprot.org/uniprot/Q62353"},{"id":"T6","span":{"begin":1059,"end":1061},"obj":"http://www.uniprot.org/uniprot/P06684"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"},{"subj":"T2","pred":"source","obj":"uniprot-mouse"},{"subj":"T3","pred":"source","obj":"uniprot-mouse"},{"subj":"T4","pred":"source","obj":"uniprot-mouse"},{"subj":"T5","pred":"source","obj":"uniprot-mouse"},{"subj":"T6","pred":"source","obj":"uniprot-mouse"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":62,"end":69},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T2","span":{"begin":159,"end":166},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T3","span":{"begin":1832,"end":1840},"obj":"http://purl.obolibrary.org/obo/GO_0051923"},{"id":"T4","span":{"begin":644,"end":653},"obj":"http://purl.obolibrary.org/obo/GO_0032502"},{"id":"T5","span":{"begin":681,"end":690},"obj":"http://purl.obolibrary.org/obo/GO_0046960"},{"id":"T6","span":{"begin":1131,"end":1140},"obj":"http://purl.obolibrary.org/obo/GO_0046960"},{"id":"T7","span":{"begin":822,"end":833},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T8","span":{"begin":922,"end":928},"obj":"http://purl.obolibrary.org/obo/GO_0060361"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"}]},{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":6,"end":16},"obj":"http://edamontology.org/topic_3168"},{"id":"T2","span":{"begin":6,"end":16},"obj":"http://edamontology.org/topic_0080"},{"id":"T3","span":{"begin":279,"end":313},"obj":"http://edamontology.org/topic_0154"},{"id":"T4","span":{"begin":290,"end":313},"obj":"http://edamontology.org/topic_0114"},{"id":"T5","span":{"begin":453,"end":463},"obj":"http://edamontology.org/topic_3168"},{"id":"T6","span":{"begin":453,"end":463},"obj":"http://edamontology.org/topic_0080"},{"id":"T7","span":{"begin":634,"end":639},"obj":"http://edamontology.org/topic_3678"},{"id":"T8","span":{"begin":706,"end":714},"obj":"http://edamontology.org/topic_3168"},{"id":"T9","span":{"begin":706,"end":714},"obj":"http://edamontology.org/topic_0080"},{"id":"T10","span":{"begin":706,"end":723},"obj":"http://edamontology.org/topic_0080"},{"id":"T11","span":{"begin":929,"end":946},"obj":"http://edamontology.org/topic_0134"},{"id":"T12","span":{"begin":929,"end":946},"obj":"http://edamontology.org/topic_3520"},{"id":"T13","span":{"begin":1364,"end":1372},"obj":"http://edamontology.org/topic_3168"},{"id":"T14","span":{"begin":1364,"end":1372},"obj":"http://edamontology.org/topic_0080"},{"id":"T15","span":{"begin":1471,"end":1479},"obj":"http://edamontology.org/topic_3168"},{"id":"T16","span":{"begin":1471,"end":1479},"obj":"http://edamontology.org/topic_0080"},{"id":"T17","span":{"begin":1693,"end":1703},"obj":"http://edamontology.org/topic_3168"},{"id":"T18","span":{"begin":1693,"end":1703},"obj":"http://edamontology.org/topic_0080"},{"id":"T19","span":{"begin":1816,"end":1826},"obj":"http://edamontology.org/topic_3168"},{"id":"T20","span":{"begin":1816,"end":1826},"obj":"http://edamontology.org/topic_0080"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-topics"},{"subj":"T2","pred":"source","obj":"EDAM-topics"},{"subj":"T3","pred":"source","obj":"EDAM-topics"},{"subj":"T4","pred":"source","obj":"EDAM-topics"},{"subj":"T5","pred":"source","obj":"EDAM-topics"},{"subj":"T6","pred":"source","obj":"EDAM-topics"},{"subj":"T7","pred":"source","obj":"EDAM-topics"},{"subj":"T8","pred":"source","obj":"EDAM-topics"},{"subj":"T9","pred":"source","obj":"EDAM-topics"},{"subj":"T10","pred":"source","obj":"EDAM-topics"},{"subj":"T11","pred":"source","obj":"EDAM-topics"},{"subj":"T12","pred":"source","obj":"EDAM-topics"},{"subj":"T13","pred":"source","obj":"EDAM-topics"},{"subj":"T14","pred":"source","obj":"EDAM-topics"},{"subj":"T15","pred":"source","obj":"EDAM-topics"},{"subj":"T16","pred":"source","obj":"EDAM-topics"},{"subj":"T17","pred":"source","obj":"EDAM-topics"},{"subj":"T18","pred":"source","obj":"EDAM-topics"},{"subj":"T19","pred":"source","obj":"EDAM-topics"},{"subj":"T20","pred":"source","obj":"EDAM-topics"}]},{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":6,"end":16},"obj":"http://edamontology.org/data_2044"},{"id":"T2","span":{"begin":6,"end":16},"obj":"http://edamontology.org/operation_3218"},{"id":"T3","span":{"begin":139,"end":147},"obj":"http://edamontology.org/data_1756"},{"id":"T4","span":{"begin":290,"end":299},"obj":"http://edamontology.org/operation_3429"},{"id":"T5","span":{"begin":301,"end":313},"obj":"http://edamontology.org/data_0883"},{"id":"T6","span":{"begin":453,"end":463},"obj":"http://edamontology.org/operation_3218"},{"id":"T7","span":{"begin":453,"end":463},"obj":"http://edamontology.org/data_2044"},{"id":"T8","span":{"begin":490,"end":499},"obj":"http://edamontology.org/operation_3429"},{"id":"T9","span":{"begin":607,"end":616},"obj":"http://edamontology.org/operation_2423"},{"id":"T10","span":{"begin":658,"end":667},"obj":"http://edamontology.org/operation_2428"},{"id":"T11","span":{"begin":706,"end":714},"obj":"http://edamontology.org/operation_3218"},{"id":"T12","span":{"begin":706,"end":714},"obj":"http://edamontology.org/data_2044"},{"id":"T13","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_0258"},{"id":"T14","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_2403"},{"id":"T15","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_3197"},{"id":"T16","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_3229"},{"id":"T17","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_2404"},{"id":"T18","span":{"begin":706,"end":723},"obj":"http://edamontology.org/operation_2408"},{"id":"T19","span":{"begin":715,"end":723},"obj":"http://edamontology.org/operation_2945"},{"id":"T20","span":{"begin":795,"end":797},"obj":"http://edamontology.org/format_1997"},{"id":"T21","span":{"begin":863,"end":867},"obj":"http://edamontology.org/format_1996"},{"id":"T22","span":{"begin":877,"end":882},"obj":"http://edamontology.org/operation_3454"},{"id":"T23","span":{"begin":877,"end":882},"obj":"http://edamontology.org/data_2336"},{"id":"T24","span":{"begin":929,"end":946},"obj":"http://edamontology.org/data_2536"},{"id":"T25","span":{"begin":929,"end":946},"obj":"http://edamontology.org/data_3147"},{"id":"T26","span":{"begin":1016,"end":1024},"obj":"http://edamontology.org/operation_3435"},{"id":"T27","span":{"begin":1043,"end":1052},"obj":"http://edamontology.org/operation_2423"},{"id":"T28","span":{"begin":1164,"end":1170},"obj":"http://edamontology.org/operation_2423"},{"id":"T29","span":{"begin":1243,"end":1251},"obj":"http://edamontology.org/operation_2945"},{"id":"T30","span":{"begin":1268,"end":1276},"obj":"http://edamontology.org/data_1756"},{"id":"T31","span":{"begin":1325,"end":1335},"obj":"http://edamontology.org/data_0883"},{"id":"T32","span":{"begin":1364,"end":1372},"obj":"http://edamontology.org/data_2044"},{"id":"T33","span":{"begin":1364,"end":1372},"obj":"http://edamontology.org/operation_3218"},{"id":"T34","span":{"begin":1471,"end":1479},"obj":"http://edamontology.org/data_2044"},{"id":"T35","span":{"begin":1471,"end":1479},"obj":"http://edamontology.org/operation_3218"},{"id":"T36","span":{"begin":1504,"end":1513},"obj":"http://edamontology.org/operation_3429"},{"id":"T37","span":{"begin":1583,"end":1591},"obj":"http://edamontology.org/data_1756"},{"id":"T38","span":{"begin":1693,"end":1703},"obj":"http://edamontology.org/data_2044"},{"id":"T39","span":{"begin":1693,"end":1703},"obj":"http://edamontology.org/operation_3218"},{"id":"T40","span":{"begin":1816,"end":1826},"obj":"http://edamontology.org/operation_3218"},{"id":"T41","span":{"begin":1816,"end":1826},"obj":"http://edamontology.org/data_2044"},{"id":"T42","span":{"begin":1841,"end":1849},"obj":"http://edamontology.org/data_1756"},{"id":"T43","span":{"begin":1868,"end":1870},"obj":"http://edamontology.org/format_1997"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-DFO"},{"subj":"T2","pred":"source","obj":"EDAM-DFO"},{"subj":"T3","pred":"source","obj":"EDAM-DFO"},{"subj":"T4","pred":"source","obj":"EDAM-DFO"},{"subj":"T5","pred":"source","obj":"EDAM-DFO"},{"subj":"T6","pred":"source","obj":"EDAM-DFO"},{"subj":"T7","pred":"source","obj":"EDAM-DFO"},{"subj":"T8","pred":"source","obj":"EDAM-DFO"},{"subj":"T9","pred":"source","obj":"EDAM-DFO"},{"subj":"T10","pred":"source","obj":"EDAM-DFO"},{"subj":"T11","pred":"source","obj":"EDAM-DFO"},{"subj":"T12","pred":"source","obj":"EDAM-DFO"},{"subj":"T13","pred":"source","obj":"EDAM-DFO"},{"subj":"T14","pred":"source","obj":"EDAM-DFO"},{"subj":"T15","pred":"source","obj":"EDAM-DFO"},{"subj":"T16","pred":"source","obj":"EDAM-DFO"},{"subj":"T17","pred":"source","obj":"EDAM-DFO"},{"subj":"T18","pred":"source","obj":"EDAM-DFO"},{"subj":"T19","pred":"source","obj":"EDAM-DFO"},{"subj":"T20","pred":"source","obj":"EDAM-DFO"},{"subj":"T21","pred":"source","obj":"EDAM-DFO"},{"subj":"T22","pred":"source","obj":"EDAM-DFO"},{"subj":"T23","pred":"source","obj":"EDAM-DFO"},{"subj":"T24","pred":"source","obj":"EDAM-DFO"},{"subj":"T25","pred":"source","obj":"EDAM-DFO"},{"subj":"T26","pred":"source","obj":"EDAM-DFO"},{"subj":"T27","pred":"source","obj":"EDAM-DFO"},{"subj":"T28","pred":"source","obj":"EDAM-DFO"},{"subj":"T29","pred":"source","obj":"EDAM-DFO"},{"subj":"T30","pred":"source","obj":"EDAM-DFO"},{"subj":"T31","pred":"source","obj":"EDAM-DFO"},{"subj":"T32","pred":"source","obj":"EDAM-DFO"},{"subj":"T33","pred":"source","obj":"EDAM-DFO"},{"subj":"T34","pred":"source","obj":"EDAM-DFO"},{"subj":"T35","pred":"source","obj":"EDAM-DFO"},{"subj":"T36","pred":"source","obj":"EDAM-DFO"},{"subj":"T37","pred":"source","obj":"EDAM-DFO"},{"subj":"T38","pred":"source","obj":"EDAM-DFO"},{"subj":"T39","pred":"source","obj":"EDAM-DFO"},{"subj":"T40","pred":"source","obj":"EDAM-DFO"},{"subj":"T41","pred":"source","obj":"EDAM-DFO"},{"subj":"T42","pred":"source","obj":"EDAM-DFO"},{"subj":"T43","pred":"source","obj":"EDAM-DFO"}]},{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":46,"end":53},"obj":"http://rdf.glycoinfo.org/glycan/G54161DR"},{"id":"T2","span":{"begin":339,"end":346},"obj":"http://rdf.glycoinfo.org/glycan/G54161DR"},{"id":"T3","span":{"begin":743,"end":750},"obj":"http://rdf.glycoinfo.org/glycan/G54161DR"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T2","pred":"source","obj":"GlycoBiology-Motifs"},{"subj":"T3","pred":"source","obj":"GlycoBiology-Motifs"}]},{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":54,"end":69},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":151,"end":166},"obj":"http://www.glycoepitope.jp/epitopes/EP0086"}],"attributes":[{"subj":"PD-GlycoEpitope-B_T1","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T2","pred":"source","obj":"GlycoBiology-Epitope"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Glycan-Motif","color":"#93ecc0","default":true},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#eca693"},{"id":"Glycosmos6-GlycoEpitope","color":"#9399ec"},{"id":"sentences","color":"#b3ec93"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#ec93cd"},{"id":"GlycoBiology-GDGDB","color":"#93e7ec"},{"id":"GlycoBiology-FMA","color":"#ecd693"},{"id":"uniprot-human","color":"#bc93ec"},{"id":"uniprot-mouse","color":"#93eca2"},{"id":"GO-BP","color":"#ec939d"},{"id":"EDAM-topics","color":"#93b7ec"},{"id":"EDAM-DFO","color":"#d1ec93"},{"id":"GlycoBiology-Motifs","color":"#ec93eb"},{"id":"GlycoBiology-Epitope","color":"#93ecd3"}]}]}}