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PubMed:25677303 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 46-53 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 46-53 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 339-346 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T4 339-346 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T5 743-750 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T6 743-750 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 46-53 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T3 339-346 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T5 743-750 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 54-69 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate
T2 151-166 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate
T3 168-170 http://www.glycoepitope.jp/epitopes/EP0086 denotes HS
T4 347-349 http://www.glycoepitope.jp/epitopes/EP0086 denotes HS
T5 436-438 http://www.glycoepitope.jp/epitopes/EP0086 denotes HS
T6 751-753 http://www.glycoepitope.jp/epitopes/EP0086 denotes HS
T7 1753-1755 http://www.glycoepitope.jp/epitopes/EP0086 denotes HS

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-87 Sentence denotes A new sequencing approach for N-unsubstituted heparin/heparan sulfate oligosaccharides.
TextSentencer_T2 88-227 Sentence denotes The rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles.
TextSentencer_T3 228-439 Sentence denotes Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS.
TextSentencer_T4 440-625 Sentence denotes However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods.
TextSentencer_T5 626-771 Sentence denotes In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides.
TextSentencer_T6 772-966 Sentence denotes This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS).
TextSentencer_T7 967-1277 Sentence denotes We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues.
TextSentencer_T8 1278-1419 Sentence denotes The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide.
TextSentencer_T9 1420-1649 Sentence denotes This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations.
TextSentencer_T10 1650-1891 Sentence denotes This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.
T1 0-87 Sentence denotes A new sequencing approach for N-unsubstituted heparin/heparan sulfate oligosaccharides.
T2 88-227 Sentence denotes The rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles.
T3 228-439 Sentence denotes Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS.
T4 440-625 Sentence denotes However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods.
T5 626-771 Sentence denotes In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides.
T6 772-966 Sentence denotes This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS).
T7 967-1277 Sentence denotes We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues.
T8 1278-1419 Sentence denotes The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide.
T9 1420-1649 Sentence denotes This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations.
T10 1650-1891 Sentence denotes This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.
T1 0-87 Sentence denotes A new sequencing approach for N-unsubstituted heparin/heparan sulfate oligosaccharides.
T2 88-227 Sentence denotes The rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles.
T3 228-439 Sentence denotes Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS.
T4 440-625 Sentence denotes However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods.
T5 626-771 Sentence denotes In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides.
T6 772-966 Sentence denotes This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS).
T7 967-1277 Sentence denotes We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues.
T8 1278-1419 Sentence denotes The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide.
T9 1420-1649 Sentence denotes This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations.
T10 1650-1891 Sentence denotes This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 46-53 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 46-53 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 339-346 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T4 339-346 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T5 743-750 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T6 743-750 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

GlycoBiology-GDGDB

Id Subject Object Predicate Lexical cue
_T1 1012-1014 http://acgg.asia/db/diseases/gdgdb?con_ui=CON00386 denotes nm

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 46-53 FMAID:167420 denotes heparin
_T2 46-53 FMAID:82839 denotes heparin
_T3 54-61 FMAID:67110 denotes heparan
_T4 54-61 FMAID:165191 denotes heparan
_T5 54-69 FMAID:63023 denotes heparan sulfate
_T6 54-69 FMAID:167405 denotes heparan sulfate
_T7 70-86 FMAID:196731 denotes oligosaccharides
_T8 70-86 FMAID:82742 denotes oligosaccharides
_T9 113-124 FMAID:196792 denotes glucosamine
_T10 113-124 FMAID:82797 denotes glucosamine
_T11 151-158 FMAID:165191 denotes heparan
_T12 151-158 FMAID:67110 denotes heparan
_T13 151-166 FMAID:63023 denotes heparan sulfate
_T14 151-166 FMAID:167405 denotes heparan sulfate
_T15 339-346 FMAID:82839 denotes heparin
_T16 339-346 FMAID:167420 denotes heparin
_T17 350-366 FMAID:196731 denotes oligosaccharides
_T18 350-366 FMAID:82742 denotes oligosaccharides
_T19 500-516 FMAID:196731 denotes oligosaccharides
_T20 500-516 FMAID:82742 denotes oligosaccharides
_T21 670-680 FMAID:62083 denotes simple and
_T22 670-680 FMAID:165655 denotes simple and
_T23 743-750 FMAID:167420 denotes heparin
_T24 743-750 FMAID:82839 denotes heparin
_T25 754-770 FMAID:82742 denotes oligosaccharides
_T26 754-770 FMAID:196731 denotes oligosaccharides
_T27 883-889 FMAID:85815 denotes liquid
_T28 1056-1058 FMAID:63497 denotes C4
_T29 1056-1058 FMAID:167722 denotes C4
_T30 1059-1061 FMAID:167723 denotes C5
_T31 1059-1061 FMAID:63498 denotes C5
_T32 1074-1090 FMAID:196731 denotes oligosaccharides
_T33 1074-1090 FMAID:82742 denotes oligosaccharides
_T34 1181-1197 FMAID:82742 denotes oligosaccharides
_T35 1181-1197 FMAID:196731 denotes oligosaccharides
_T36 1403-1418 FMAID:196731 denotes oligosaccharide
_T37 1403-1418 FMAID:82742 denotes oligosaccharide
_T38 1596-1611 FMAID:196731 denotes oligosaccharide
_T39 1596-1611 FMAID:82742 denotes oligosaccharide
_T40 1731-1747 FMAID:196731 denotes oligosaccharides
_T41 1731-1747 FMAID:82742 denotes oligosaccharides

uniprot-human

Id Subject Object Predicate Lexical cue
T1 909-913 http://www.uniprot.org/uniprot/Q9UFF0 denotes trap
T2 962-964 http://www.uniprot.org/uniprot/Q99707 denotes MS
T3 1296-1298 http://www.uniprot.org/uniprot/Q99707 denotes MS
T4 1059-1061 http://www.uniprot.org/uniprot/P01031 denotes C5

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 909-913 http://www.uniprot.org/uniprot/Q05117 denotes trap
T2 909-913 http://www.uniprot.org/uniprot/Q8K1H1 denotes trap
T3 962-964 http://www.uniprot.org/uniprot/A6H5Y3 denotes MS
T4 1296-1298 http://www.uniprot.org/uniprot/A6H5Y3 denotes MS
T5 1056-1058 http://www.uniprot.org/uniprot/Q62353 denotes C4
T6 1059-1061 http://www.uniprot.org/uniprot/P06684 denotes C5

GO-BP

Id Subject Object Predicate Lexical cue
T1 62-69 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T2 159-166 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T3 1832-1840 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfated
T4 644-653 http://purl.obolibrary.org/obo/GO_0032502 denotes developed
T5 681-690 http://purl.obolibrary.org/obo/GO_0046960 denotes sensitive
T6 1131-1140 http://purl.obolibrary.org/obo/GO_0046960 denotes sensitive
T7 822-833 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T8 922-928 http://purl.obolibrary.org/obo/GO_0060361 denotes flight

EDAM-topics

Id Subject Object Predicate Lexical cue
T1 6-16 http://edamontology.org/topic_3168 denotes sequencing
T2 6-16 http://edamontology.org/topic_0080 denotes sequencing
T3 279-313 http://edamontology.org/topic_0154 denotes chemically generated, structurally
T4 290-313 http://edamontology.org/topic_0114 denotes generated, structurally
T5 453-463 http://edamontology.org/topic_3168 denotes sequencing
T6 453-463 http://edamontology.org/topic_0080 denotes sequencing
T7 634-639 http://edamontology.org/topic_3678 denotes study
T8 706-714 http://edamontology.org/topic_3168 denotes sequence
T9 706-714 http://edamontology.org/topic_0080 denotes sequence
T10 706-723 http://edamontology.org/topic_0080 denotes sequence analysis
T11 929-946 http://edamontology.org/topic_0134 denotes mass spectrometry
T12 929-946 http://edamontology.org/topic_3520 denotes mass spectrometry
T13 1364-1372 http://edamontology.org/topic_3168 denotes sequence
T14 1364-1372 http://edamontology.org/topic_0080 denotes sequence
T15 1471-1479 http://edamontology.org/topic_3168 denotes sequence
T16 1471-1479 http://edamontology.org/topic_0080 denotes sequence
T17 1693-1703 http://edamontology.org/topic_3168 denotes sequencing
T18 1693-1703 http://edamontology.org/topic_0080 denotes sequencing
T19 1816-1826 http://edamontology.org/topic_3168 denotes sequencing
T20 1816-1826 http://edamontology.org/topic_0080 denotes sequencing

EDAM-DFO

Id Subject Object Predicate Lexical cue
T1 6-16 http://edamontology.org/data_2044 denotes sequencing
T2 6-16 http://edamontology.org/operation_3218 denotes sequencing
T3 139-147 http://edamontology.org/data_1756 denotes residues
T4 290-299 http://edamontology.org/operation_3429 denotes generated
T5 301-313 http://edamontology.org/data_0883 denotes structurally
T6 453-463 http://edamontology.org/operation_3218 denotes sequencing
T7 453-463 http://edamontology.org/data_2044 denotes sequencing
T8 490-499 http://edamontology.org/operation_3429 denotes generated
T9 607-616 http://edamontology.org/operation_2423 denotes detection
T10 658-667 http://edamontology.org/operation_2428 denotes validated
T11 706-714 http://edamontology.org/operation_3218 denotes sequence
T12 706-714 http://edamontology.org/data_2044 denotes sequence
T13 706-723 http://edamontology.org/operation_0258 denotes sequence analysis
T14 706-723 http://edamontology.org/operation_2403 denotes sequence analysis
T15 706-723 http://edamontology.org/operation_3197 denotes sequence analysis
T16 706-723 http://edamontology.org/operation_3229 denotes sequence analysis
T17 706-723 http://edamontology.org/operation_2404 denotes sequence analysis
T18 706-723 http://edamontology.org/operation_2408 denotes sequence analysis
T19 715-723 http://edamontology.org/operation_2945 denotes analysis
T20 795-797 http://edamontology.org/format_1997 denotes pH
T21 863-867 http://edamontology.org/format_1996 denotes pair
T22 877-882 http://edamontology.org/operation_3454 denotes phase
T23 877-882 http://edamontology.org/data_2336 denotes phase
T24 929-946 http://edamontology.org/data_2536 denotes mass spectrometry
T25 929-946 http://edamontology.org/data_3147 denotes mass spectrometry
T26 1016-1024 http://edamontology.org/operation_3435 denotes normally
T27 1043-1052 http://edamontology.org/operation_2423 denotes detection
T28 1164-1170 http://edamontology.org/operation_2423 denotes detect
T29 1243-1251 http://edamontology.org/operation_2945 denotes analysis
T30 1268-1276 http://edamontology.org/data_1756 denotes residues
T31 1325-1335 http://edamontology.org/data_0883 denotes structural
T32 1364-1372 http://edamontology.org/data_2044 denotes sequence
T33 1364-1372 http://edamontology.org/operation_3218 denotes sequence
T34 1471-1479 http://edamontology.org/data_2044 denotes sequence
T35 1471-1479 http://edamontology.org/operation_3218 denotes sequence
T36 1504-1513 http://edamontology.org/operation_3429 denotes generated
T37 1583-1591 http://edamontology.org/data_1756 denotes residues
T38 1693-1703 http://edamontology.org/data_2044 denotes sequencing
T39 1693-1703 http://edamontology.org/operation_3218 denotes sequencing
T40 1816-1826 http://edamontology.org/operation_3218 denotes sequencing
T41 1816-1826 http://edamontology.org/data_2044 denotes sequencing
T42 1841-1849 http://edamontology.org/data_1756 denotes residues
T43 1868-1870 http://edamontology.org/format_1997 denotes pH

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 46-53 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T2 339-346 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin
T3 743-750 http://rdf.glycoinfo.org/glycan/G54161DR denotes heparin

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 54-69 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate
PD-GlycoEpitope-B_T2 151-166 http://www.glycoepitope.jp/epitopes/EP0086 denotes heparan sulfate

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-87 Sentence denotes A new sequencing approach for N-unsubstituted heparin/heparan sulfate oligosaccharides.
T2 88-227 Sentence denotes The rare N-unsubstituted glucosamine (GlcNH(3)(+)) residues in heparan sulfate (HS) have important biological and pathophysiological roles.
T3 228-439 Sentence denotes Because of their low natural abundance, the use of chemically generated, structurally defined, N-unsubstituted heparin/HS oligosaccharides can greatly contribute to the investigation of their natural role in HS.
T4 440-625 Sentence denotes However, the sequencing of mixtures of chemically generated oligosaccharides presents major challenges due to the difficulties in separating isomers and the available detection methods.
T5 626-771 Sentence denotes In this study, we developed and validated a simple and sensitive method for the sequence analysis of N-unsubstituted heparin/HS oligosaccharides.
T6 772-966 Sentence denotes This protocol involves pH 4 nitrous acid (HNO(2)) degradation, size-exclusion HPLC and ion-pair reversed-phase liquid chromatography-ion trap/time-of-flight mass spectrometry (IPRP-LC-ITTOF MS).
T7 967-1277 Sentence denotes We unexpectedly found that absorbance at 232 nm (normally used for specific detection of C4-C5 unsaturated oligosaccharides) was, in most cases, still sufficiently sensitive to also simultaneously detect saturated oligosaccharides during HPLC, thus simplifying the positional analysis of GlcNH(3)(+)) residues.
T8 1278-1419 Sentence denotes The IPRP-LC-ITTOF MS system can supply further structural information leading to full sequence determination of the original oligosaccharide.
T9 1420-1649 Sentence denotes This new methodology has been used to separate and sequence a variety of chemically generated, N-unsubstituted dp6 species containing between 1 and 3 GlcNH(3)(+)) residues per oligosaccharide in different positional combinations.
T10 1650-1891 Sentence denotes This strategy offers possibilities for the sequencing of natural N-unsubstituted oligosaccharides from HS and should also be applicable, with minor modification, for sequencing at N-sulfated residues using alternative pH 1.5 HNO(2) scission.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 54-69 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086
T2 151-166 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes heparan sulfate http://www.glycoepitope.jp/epitopes/EP0086
T3 168-170 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes HS http://www.glycoepitope.jp/epitopes/EP0086
T4 347-349 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes HS http://www.glycoepitope.jp/epitopes/EP0086
T5 436-438 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes HS http://www.glycoepitope.jp/epitopes/EP0086
T6 751-753 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes HS http://www.glycoepitope.jp/epitopes/EP0086
T7 1753-1755 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes HS http://www.glycoepitope.jp/epitopes/EP0086