PubMed:25393740 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25393740","sourcedb":"PubMed","sourceid":"25393740","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/25393740","text":"Cerebral artery alpha-1 AR subtypes: high altitude long-term acclimatization responses.\nIn response to hypoxia and other stress, the sympathetic (adrenergic) nervous system regulates arterial contractility and blood flow, partly through differential activities of the alpha1 (α1) - adrenergic receptor (AR) subtypes (α1A-, α1B-, and α1D-AR). Thus, we tested the hypothesis that with acclimatization to long-term hypoxia (LTH), contractility of middle cerebral arteries (MCA) is regulated by changes in expression and activation of the specific α1-AR subtypes. We conducted experiments in MCA from adult normoxic sheep maintained near sea level (300 m) and those exposed to LTH (110 days at 3801 m). Following acclimatization to LTH, ovine MCA showed a 20% reduction (n = 5; P\u003c0.05) in the maximum tension achieved by 10-5 M phenylephrine (PHE). LTH-acclimatized cerebral arteries also demonstrated a statistically significant (P\u003c0.05) inhibition of PHE-induced contractility in the presence of specific α1-AR subtype antagonists. Importantly, compared to normoxic vessels, there was significantly greater (P\u003c0.05) α1B-AR subtype mRNA and protein levels in LTH acclimatized MCA. Also, our results demonstrate that extracellular regulated kinase 1 and 2 (ERK1/2)-mediated negative feedback regulation of PHE-induced contractility is modulated by α1B-AR subtype. Overall, in ovine MCA, LTH produces profound effects on α1-AR subtype expression and function.","tracks":[{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":88,"end":341},"obj":"DRI_Background"},{"id":"T2","span":{"begin":342,"end":559},"obj":"DRI_Approach"},{"id":"T3","span":{"begin":560,"end":698},"obj":"DRI_Approach"},{"id":"T4","span":{"begin":699,"end":844},"obj":"DRI_Approach"},{"id":"T5","span":{"begin":845,"end":1029},"obj":"DRI_Outcome"},{"id":"T6","span":{"begin":1030,"end":1177},"obj":"DRI_Outcome"},{"id":"T7","span":{"begin":1178,"end":1359},"obj":"DRI_Outcome"},{"id":"T8","span":{"begin":1360,"end":1454},"obj":"DRI_Background"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T2","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T3","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T4","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T5","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T6","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T7","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T8","pred":"source","obj":"PubMed_ArguminSci"}]},{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T1","span":{"begin":250,"end":260},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T2","span":{"begin":319,"end":320},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T3","span":{"begin":325,"end":326},"obj":"CVCL_6480|Finite_cell_line|Mus musculus"},{"id":"T4","span":{"begin":335,"end":339},"obj":"CVCL_W749|Transformed_cell_line|Homo sapiens"},{"id":"T5","span":{"begin":348,"end":350},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T6","span":{"begin":470,"end":473},"obj":"CVCL_ZC21|Cancer_cell_line|Homo sapiens"},{"id":"T7","span":{"begin":491,"end":498},"obj":"CVCL_0238|Cancer_cell_line|Homo sapiens"},{"id":"T8","span":{"begin":517,"end":527},"obj":"CVCL_C410|Hybridoma|Mus musculus"},{"id":"T9","span":{"begin":560,"end":562},"obj":"CVCL_5M23|Cancer_cell_line|Mesocricetus auratus"},{"id":"T10","span":{"begin":588,"end":591},"obj":"CVCL_ZC21|Cancer_cell_line|Homo sapiens"},{"id":"T11","span":{"begin":739,"end":742},"obj":"CVCL_ZC21|Cancer_cell_line|Homo sapiens"},{"id":"T12","span":{"begin":750,"end":754},"obj":"CVCL_1940|Cancer_cell_line|Mus musculus"},{"id":"T13","span":{"begin":750,"end":754},"obj":"CVCL_Z353|Spontaneously_immortalized_cell_line|Aedes aegypti"},{"id":"T14","span":{"begin":750,"end":751},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T15","span":{"begin":752,"end":754},"obj":"CVCL_J923|Hybridoma|Mus musculus"},{"id":"T16","span":{"begin":898,"end":899},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T17","span":{"begin":1116,"end":1117},"obj":"CVCL_6480|Finite_cell_line|Mus musculus"},{"id":"T18","span":{"begin":1173,"end":1176},"obj":"CVCL_ZC21|Cancer_cell_line|Homo sapiens"},{"id":"T19","span":{"begin":1246,"end":1251},"obj":"CVCL_B527|Embryonic_stem_cell|Homo sapiens"},{"id":"T20","span":{"begin":1346,"end":1347},"obj":"CVCL_6480|Finite_cell_line|Mus musculus"},{"id":"T21","span":{"begin":1378,"end":1381},"obj":"CVCL_ZC21|Cancer_cell_line|Homo sapiens"}],"attributes":[{"subj":"T1","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T2","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T3","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T4","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T5","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T6","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T7","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T8","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T9","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T10","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T11","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T12","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T13","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T14","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T15","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T16","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T17","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T18","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T19","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T20","pred":"source","obj":"Goldhamster2_Cellosaurus"},{"subj":"T21","pred":"source","obj":"Goldhamster2_Cellosaurus"}]},{"project":"GoldHamster","denotations":[{"id":"T2","span":{"begin":0,"end":15},"obj":"UBERON:0004449"},{"id":"T1","span":{"begin":0,"end":15},"obj":"D002539"},{"id":"T3","span":{"begin":0,"end":15},"obj":"D002539"},{"id":"T5","span":{"begin":16,"end":21},"obj":"CHEBI:30216"},{"id":"T6","span":{"begin":16,"end":23},"obj":"PR:P0CY06"},{"id":"T7","span":{"begin":56,"end":60},"obj":"original_id"},{"id":"T11","span":{"begin":91,"end":110},"obj":"GO:0001666"},{"id":"T12","span":{"begin":103,"end":110},"obj":"D000860"},{"id":"T13","span":{"begin":103,"end":110},"obj":"D000860"},{"id":"T15","span":{"begin":158,"end":172},"obj":"UBERON:0001016"},{"id":"T14","span":{"begin":158,"end":172},"obj":"D009422"},{"id":"T16","span":{"begin":158,"end":172},"obj":"D009422"},{"id":"T17","span":{"begin":268,"end":273},"obj":"CHEBI:30216"},{"id":"T18","span":{"begin":268,"end":274},"obj":"PR:P0CY06"},{"id":"T23","span":{"begin":282,"end":301},"obj":"D011941"},{"id":"T41","span":{"begin":407,"end":411},"obj":"original_id"},{"id":"T42","span":{"begin":412,"end":419},"obj":"D000860"},{"id":"T43","span":{"begin":412,"end":419},"obj":"D000860"},{"id":"T44","span":{"begin":460,"end":468},"obj":"UBERON:0001637"},{"id":"T46","span":{"begin":470,"end":473},"obj":"P60254"},{"id":"T45","span":{"begin":470,"end":473},"obj":"CHEBI:34342"},{"id":"T47","span":{"begin":478,"end":487},"obj":"SO:0000119"},{"id":"T53","span":{"begin":588,"end":591},"obj":"P60254"},{"id":"T52","span":{"begin":588,"end":591},"obj":"CHEBI:34342"},{"id":"T54","span":{"begin":597,"end":602},"obj":"UBERON:0007023"},{"id":"T55","span":{"begin":612,"end":617},"obj":"9940"},{"id":"T56","span":{"begin":612,"end":617},"obj":"D012756"},{"id":"T57","span":{"begin":634,"end":637},"obj":"PR:000000735"},{"id":"T58","span":{"begin":733,"end":738},"obj":"D012757"},{"id":"T59","span":{"begin":733,"end":738},"obj":"D012757"},{"id":"T61","span":{"begin":739,"end":742},"obj":"P60254"},{"id":"T60","span":{"begin":739,"end":742},"obj":"CHEBI:34342"},{"id":"T64","span":{"begin":756,"end":765},"obj":"MOP:0000569"},{"id":"T67","span":{"begin":824,"end":837},"obj":"8163"},{"id":"T65","span":{"begin":824,"end":837},"obj":"D010656"},{"id":"T66","span":{"begin":824,"end":837},"obj":"CHEBI:8093"},{"id":"T68","span":{"begin":824,"end":837},"obj":"D010656"},{"id":"T71","span":{"begin":839,"end":842},"obj":"SO:0001441"},{"id":"T69","span":{"begin":839,"end":842},"obj":"CHEBI:32501"},{"id":"T70","span":{"begin":839,"end":842},"obj":"CHEBI:29997"},{"id":"T72","span":{"begin":839,"end":842},"obj":"CHEBI:17295"},{"id":"T73","span":{"begin":839,"end":842},"obj":"CHEBI:32496"},{"id":"T74","span":{"begin":839,"end":842},"obj":"CHEBI:28044"},{"id":"T75","span":{"begin":871,"end":879},"obj":"UBERON:0001637"},{"id":"T78","span":{"begin":949,"end":952},"obj":"SO:0001441"},{"id":"T76","span":{"begin":949,"end":952},"obj":"CHEBI:32501"},{"id":"T77","span":{"begin":949,"end":952},"obj":"CHEBI:29997"},{"id":"T79","span":{"begin":949,"end":952},"obj":"CHEBI:17295"},{"id":"T80","span":{"begin":949,"end":952},"obj":"CHEBI:32496"},{"id":"T81","span":{"begin":949,"end":952},"obj":"CHEBI:28044"},{"id":"T86","span":{"begin":1017,"end":1028},"obj":"CHEBI:48706"},{"id":"T97","span":{"begin":1129,"end":1133},"obj":"SO:0000234"},{"id":"T95","span":{"begin":1129,"end":1133},"obj":"D012333"},{"id":"T96","span":{"begin":1129,"end":1133},"obj":"CHEBI:33699"},{"id":"T98","span":{"begin":1138,"end":1145},"obj":"GO:0003675"},{"id":"T101","span":{"begin":1138,"end":1145},"obj":"PR:000000001"},{"id":"T102","span":{"begin":1138,"end":1145},"obj":"SO:0000104"},{"id":"T99","span":{"begin":1138,"end":1145},"obj":"CHEBI:36080"},{"id":"T100","span":{"begin":1138,"end":1145},"obj":"CHEBI:11122"},{"id":"T104","span":{"begin":1173,"end":1176},"obj":"P60254"},{"id":"T103","span":{"begin":1173,"end":1176},"obj":"CHEBI:34342"},{"id":"T105","span":{"begin":1213,"end":1226},"obj":"GO:0005576"},{"id":"T106","span":{"begin":1227,"end":1236},"obj":"SO:0000119"},{"id":"T111","span":{"begin":1253,"end":1257},"obj":"Q63844"},{"id":"T112","span":{"begin":1253,"end":1257},"obj":"P27361"},{"id":"T113","span":{"begin":1253,"end":1257},"obj":"P21708"},{"id":"T114","span":{"begin":1253,"end":1257},"obj":"PR:000000104"},{"id":"T115","span":{"begin":1253,"end":1257},"obj":"PR:P42525"},{"id":"T116","span":{"begin":1253,"end":1257},"obj":"Q5A1D3"},{"id":"T117","span":{"begin":1253,"end":1257},"obj":"P42525"},{"id":"T118","span":{"begin":1253,"end":1257},"obj":"PR:P27361"},{"id":"T119","span":{"begin":1253,"end":1257},"obj":"PR:P21708"},{"id":"T121","span":{"begin":1253,"end":1257},"obj":"PR:Q63844"},{"id":"T122","span":{"begin":1253,"end":1257},"obj":"C4YGK0"},{"id":"T123","span":{"begin":1253,"end":1257},"obj":"PR:Q5A1D3"},{"id":"T120","span":{"begin":1253,"end":1257},"obj":"GO:0004707"},{"id":"T124","span":{"begin":1288,"end":1298},"obj":"GO:0065007"},{"id":"T127","span":{"begin":1302,"end":1305},"obj":"SO:0001441"},{"id":"T125","span":{"begin":1302,"end":1305},"obj":"CHEBI:32501"},{"id":"T126","span":{"begin":1302,"end":1305},"obj":"CHEBI:29997"},{"id":"T128","span":{"begin":1302,"end":1305},"obj":"CHEBI:17295"},{"id":"T129","span":{"begin":1302,"end":1305},"obj":"CHEBI:32496"},{"id":"T130","span":{"begin":1302,"end":1305},"obj":"CHEBI:28044"},{"id":"T139","span":{"begin":1372,"end":1377},"obj":"D012757"},{"id":"T140","span":{"begin":1372,"end":1377},"obj":"D012757"},{"id":"T142","span":{"begin":1378,"end":1381},"obj":"P60254"},{"id":"T141","span":{"begin":1378,"end":1381},"obj":"CHEBI:34342"}],"attributes":[{"subj":"T2","pred":"source","obj":"GoldHamster"},{"subj":"T1","pred":"source","obj":"GoldHamster"},{"subj":"T3","pred":"source","obj":"GoldHamster"},{"subj":"T5","pred":"source","obj":"GoldHamster"},{"subj":"T6","pred":"source","obj":"GoldHamster"},{"subj":"T7","pred":"source","obj":"GoldHamster"},{"subj":"T11","pred":"source","obj":"GoldHamster"},{"subj":"T12","pred":"source","obj":"GoldHamster"},{"subj":"T13","pred":"source","obj":"GoldHamster"},{"subj":"T15","pred":"source","obj":"GoldHamster"},{"subj":"T14","pred":"source","obj":"GoldHamster"},{"subj":"T16","pred":"source","obj":"GoldHamster"},{"subj":"T17","pred":"source","obj":"GoldHamster"},{"subj":"T18","pred":"source","obj":"GoldHamster"},{"subj":"T23","pred":"source","obj":"GoldHamster"},{"subj":"T41","pred":"source","obj":"GoldHamster"},{"subj":"T42","pred":"source","obj":"GoldHamster"},{"subj":"T43","pred":"source","obj":"GoldHamster"},{"subj":"T44","pred":"source","obj":"GoldHamster"},{"subj":"T46","pred":"source","obj":"GoldHamster"},{"subj":"T45","pred":"source","obj":"GoldHamster"},{"subj":"T47","pred":"source","obj":"GoldHamster"},{"subj":"T53","pred":"source","obj":"GoldHamster"},{"subj":"T52","pred":"source","obj":"GoldHamster"},{"subj":"T54","pred":"source","obj":"GoldHamster"},{"subj":"T55","pred":"source","obj":"GoldHamster"},{"subj":"T56","pred":"source","obj":"GoldHamster"},{"subj":"T57","pred":"source","obj":"GoldHamster"},{"subj":"T58","pred":"source","obj":"GoldHamster"},{"subj":"T59","pred":"source","obj":"GoldHamster"},{"subj":"T61","pred":"source","obj":"GoldHamster"},{"subj":"T60","pred":"source","obj":"GoldHamster"},{"subj":"T64","pred":"source","obj":"GoldHamster"},{"subj":"T67","pred":"source","obj":"GoldHamster"},{"subj":"T65","pred":"source","obj":"GoldHamster"},{"subj":"T66","pred":"source","obj":"GoldHamster"},{"subj":"T68","pred":"source","obj":"GoldHamster"},{"subj":"T71","pred":"source","obj":"GoldHamster"},{"subj":"T69","pred":"source","obj":"GoldHamster"},{"subj":"T70","pred":"source","obj":"GoldHamster"},{"subj":"T72","pred":"source","obj":"GoldHamster"},{"subj":"T73","pred":"source","obj":"GoldHamster"},{"subj":"T74","pred":"source","obj":"GoldHamster"},{"subj":"T75","pred":"source","obj":"GoldHamster"},{"subj":"T78","pred":"source","obj":"GoldHamster"},{"subj":"T76","pred":"source","obj":"GoldHamster"},{"subj":"T77","pred":"source","obj":"GoldHamster"},{"subj":"T79","pred":"source","obj":"GoldHamster"},{"subj":"T80","pred":"source","obj":"GoldHamster"},{"subj":"T81","pred":"source","obj":"GoldHamster"},{"subj":"T86","pred":"source","obj":"GoldHamster"},{"subj":"T97","pred":"source","obj":"GoldHamster"},{"subj":"T95","pred":"source","obj":"GoldHamster"},{"subj":"T96","pred":"source","obj":"GoldHamster"},{"subj":"T98","pred":"source","obj":"GoldHamster"},{"subj":"T101","pred":"source","obj":"GoldHamster"},{"subj":"T102","pred":"source","obj":"GoldHamster"},{"subj":"T99","pred":"source","obj":"GoldHamster"},{"subj":"T100","pred":"source","obj":"GoldHamster"},{"subj":"T104","pred":"source","obj":"GoldHamster"},{"subj":"T103","pred":"source","obj":"GoldHamster"},{"subj":"T105","pred":"source","obj":"GoldHamster"},{"subj":"T106","pred":"source","obj":"GoldHamster"},{"subj":"T111","pred":"source","obj":"GoldHamster"},{"subj":"T112","pred":"source","obj":"GoldHamster"},{"subj":"T113","pred":"source","obj":"GoldHamster"},{"subj":"T114","pred":"source","obj":"GoldHamster"},{"subj":"T115","pred":"source","obj":"GoldHamster"},{"subj":"T116","pred":"source","obj":"GoldHamster"},{"subj":"T117","pred":"source","obj":"GoldHamster"},{"subj":"T118","pred":"source","obj":"GoldHamster"},{"subj":"T119","pred":"source","obj":"GoldHamster"},{"subj":"T121","pred":"source","obj":"GoldHamster"},{"subj":"T122","pred":"source","obj":"GoldHamster"},{"subj":"T123","pred":"source","obj":"GoldHamster"},{"subj":"T120","pred":"source","obj":"GoldHamster"},{"subj":"T124","pred":"source","obj":"GoldHamster"},{"subj":"T127","pred":"source","obj":"GoldHamster"},{"subj":"T125","pred":"source","obj":"GoldHamster"},{"subj":"T126","pred":"source","obj":"GoldHamster"},{"subj":"T128","pred":"source","obj":"GoldHamster"},{"subj":"T129","pred":"source","obj":"GoldHamster"},{"subj":"T130","pred":"source","obj":"GoldHamster"},{"subj":"T139","pred":"source","obj":"GoldHamster"},{"subj":"T140","pred":"source","obj":"GoldHamster"},{"subj":"T142","pred":"source","obj":"GoldHamster"},{"subj":"T141","pred":"source","obj":"GoldHamster"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":192,"end":205},"obj":"HP_0001371"},{"id":"T2","span":{"begin":427,"end":440},"obj":"HP_0001371"},{"id":"T3","span":{"begin":961,"end":974},"obj":"HP_0001371"},{"id":"T4","span":{"begin":1314,"end":1327},"obj":"HP_0001371"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"},{"subj":"T4","pred":"source","obj":"PubmedHPO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubMed_ArguminSci","color":"#ecdb93","default":true},{"id":"Goldhamster2_Cellosaurus","color":"#c193ec"},{"id":"GoldHamster","color":"#93eca7"},{"id":"PubmedHPO","color":"#ec9398"}]}]}}