PubMed:2535495 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2535495","sourcedb":"PubMed","sourceid":"2535495","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2535495","text":"Chemical evidence for covalent linkages of a semi-synthetic glycoconjugate vaccine for Haemophilus influenzae type B disease.\nWe have defined the nature of the covalent linkages in a Haemophilus influenzae type b oligosaccharide-CRM197 conjugate vaccine, designated HbOC. The conjugate was acid hydrolyzed to release a novel amino-acid derivative, N epsilon-(2-hydroxyethyl)lysine (OHEt-Lys), identifiable with an amino-acid analyzer. This amino-acid derivative was formed by reduction of Schiff bases formed between H. influenzae type b oligosaccharides (HbO) and the lysyl epsilon-amino groups of CRM197 (a non-toxic, cross-reactive variant of diphtheria toxin), followed by acid hydrolysis of HbOC. Quantification of OHEt-Lys per CRM197 molecule allowed the determination of a covalency ratio, a useful parameter for evaluating the stoichiometry and consistency of HbOC preparations. Covalent association between HbO and CRM197 was also demonstrated by the coincidence of immunoreactivity of gel-electrophoresed HbOC on a Western blot probed with anti-CRM197 and anti-saccharide antisera.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":126,"end":271},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":272,"end":434},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":435,"end":701},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":702,"end":886},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":887,"end":1091},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":125},"obj":"Sentence"},{"id":"T2","span":{"begin":126,"end":271},"obj":"Sentence"},{"id":"T3","span":{"begin":272,"end":434},"obj":"Sentence"},{"id":"T4","span":{"begin":435,"end":701},"obj":"Sentence"},{"id":"T5","span":{"begin":702,"end":886},"obj":"Sentence"},{"id":"T6","span":{"begin":887,"end":1091},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":87,"end":109},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":183,"end":205},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"727"},{"id":"A2","pred":"db_id","subj":"T2","obj":"727"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":99,"end":109},"obj":"Disease"},{"id":"T2","span":{"begin":195,"end":205},"obj":"Disease"},{"id":"T3","span":{"begin":520,"end":530},"obj":"Disease"},{"id":"T4","span":{"begin":646,"end":656},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005812"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005504"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"},{"subj":"T4","pred":"source","obj":"mondo_disease"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":350,"end":357},"obj":"Cell"},{"id":"T2","span":{"begin":575,"end":582},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0003025"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0003025"},{"subj":"T1","pred":"source","obj":"CL-cell"},{"subj":"T2","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ec93c1","default":true},{"id":"NCBITAXON","color":"#93dbec"},{"id":"mondo_disease","color":"#ece393"},{"id":"CL-cell","color":"#c993ec"}]}]}}