PubMed:25286407 / 604-1121 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25286407","sourcedb":"PubMed","sourceid":"25286407","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/25286407","text":"For the organotypic culture model, rabbit IVDs were harvested and cultured for 3 or 7 days after intradiscal injection of local anesthetics (1% lidocaine or 0.5% bupivacaine). Nucleus pulposus (NP) cell death was measured using confocal microscopy. Histological and TUNEL assays were performed. For in vivo study, each local anesthetic was injected into rabbit lumbar IVDs under a fluoroscope. 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