Id |
Subject |
Object |
Predicate |
Lexical cue |
TextSentencer_T1 |
0-76 |
Sentence |
denotes |
miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer. |
TextSentencer_T2 |
77-88 |
Sentence |
denotes |
BACKGROUND: |
TextSentencer_T3 |
89-225 |
Sentence |
denotes |
The application of microRNAs (miRNAs) as potential biomarkers and therapy targets has been widely investigated in many kinds of cancers. |
TextSentencer_T4 |
226-370 |
Sentence |
denotes |
The discovery of tumor associated miRNAs in serum of patients supported the use of plasma/serum miRNAs as noninvasive means of cancer detection. |
TextSentencer_T5 |
371-522 |
Sentence |
denotes |
However, the aberrant expression of miRNAs in bladder cancer patients and their intensive roles and mechanisms in bladder cancer are poorly understood. |
TextSentencer_T6 |
523-531 |
Sentence |
denotes |
METHODS: |
TextSentencer_T7 |
532-993 |
Sentence |
denotes |
Taqman probe stem-loop real-time PCR was used to accurately measure the levels of miR-19a in bladder cancer cell lines, 100 pairs of bladder cancer tissues and the adjacent non-neoplastic tissues and also the plasma collected from bladder cancer patients and normal controls. miR-19a mimics and inhibitors were transfected into bladder cancer cells to investigate its role on regulating cell proliferation which was measured by CCK-8 and colony formation assay. |
TextSentencer_T8 |
994-1184 |
Sentence |
denotes |
The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid. |
TextSentencer_T9 |
1185-1351 |
Sentence |
denotes |
RESULTS: miR-19a was significantly up-regulated in bladder cancer tissues and high-level of miR-19a was correlative with more aggressive phenotypes of bladder cancer. |
TextSentencer_T10 |
1352-1573 |
Sentence |
denotes |
Meanwhile, gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN. |
TextSentencer_T11 |
1574-1841 |
Sentence |
denotes |
Furthermore, investigation of miR-19a expression in the plasma of bladder cancer patients showed that miR-19a was also increased in plasma of bladder cancer patients which strongly supported miR-19a could be developed as potential diagnostic marker of bladder cancer. |
TextSentencer_T12 |
1842-1854 |
Sentence |
denotes |
CONCLUSIONS: |
TextSentencer_T13 |
1855-2008 |
Sentence |
denotes |
Our data indicated that miR-19a might act as an oncogenic microRNA in bladder cancer and was significantly up-regulated in bladder cancer carcinogenesis. |
TextSentencer_T14 |
2009-2088 |
Sentence |
denotes |
The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN. |
T1 |
0-76 |
Sentence |
denotes |
miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer. |
T2 |
77-88 |
Sentence |
denotes |
BACKGROUND: |
T3 |
89-225 |
Sentence |
denotes |
The application of microRNAs (miRNAs) as potential biomarkers and therapy targets has been widely investigated in many kinds of cancers. |
T4 |
226-370 |
Sentence |
denotes |
The discovery of tumor associated miRNAs in serum of patients supported the use of plasma/serum miRNAs as noninvasive means of cancer detection. |
T5 |
371-522 |
Sentence |
denotes |
However, the aberrant expression of miRNAs in bladder cancer patients and their intensive roles and mechanisms in bladder cancer are poorly understood. |
T6 |
523-531 |
Sentence |
denotes |
METHODS: |
T7 |
532-993 |
Sentence |
denotes |
Taqman probe stem-loop real-time PCR was used to accurately measure the levels of miR-19a in bladder cancer cell lines, 100 pairs of bladder cancer tissues and the adjacent non-neoplastic tissues and also the plasma collected from bladder cancer patients and normal controls. miR-19a mimics and inhibitors were transfected into bladder cancer cells to investigate its role on regulating cell proliferation which was measured by CCK-8 and colony formation assay. |
T8 |
994-1184 |
Sentence |
denotes |
The target of miR-19a was identified by western blot and whether its regulatory role depends on its target was improved by a rescue experiment with miR-19a mimic and PTEN expression plasmid. |
T9 |
1185-1351 |
Sentence |
denotes |
RESULTS: miR-19a was significantly up-regulated in bladder cancer tissues and high-level of miR-19a was correlative with more aggressive phenotypes of bladder cancer. |
T10 |
1352-1573 |
Sentence |
denotes |
Meanwhile, gain or loss of function of miR-19a demonstrated that miR-19a can promote cell growth of bladder cancer cells and the further mechanism studies indicated that its oncogenic role was dependent on targeting PTEN. |
T11 |
1574-1841 |
Sentence |
denotes |
Furthermore, investigation of miR-19a expression in the plasma of bladder cancer patients showed that miR-19a was also increased in plasma of bladder cancer patients which strongly supported miR-19a could be developed as potential diagnostic marker of bladder cancer. |
T12 |
1842-1854 |
Sentence |
denotes |
CONCLUSIONS: |
T13 |
1855-2008 |
Sentence |
denotes |
Our data indicated that miR-19a might act as an oncogenic microRNA in bladder cancer and was significantly up-regulated in bladder cancer carcinogenesis. |
T14 |
2009-2088 |
Sentence |
denotes |
The oncogenic role of miR19a in bladder cancer was dependent on targeting PTEN. |