PubMed:25040827 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/25040827","sourcedb":"PubMed","sourceid":"25040827","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/25040827","text":"Structural determination of an N-glycan moiety attached to the prothoracicotropic hormone from the silkmoth Bombyx mori.\nThe predominant structure of the N-glycan on the prothoracicotropic hormone (PTTH) isolated from 1.8 million adult heads of silkmoths was determined to be Manα1-6Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc-OH, which is identical to that of the baculovirus-expressed recombinant PTTH. An ecdysis progression assay demonstrated that N-glycosylated PTTH exhibited a slightly higher activity than the recombinant PTTH without N-glycosylation produced by an Escherichia coli expression 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