PubMed:24737079 JSONTXT

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":23,"end":27},"obj":"Gene:10927"},{"id":"T10","span":{"begin":918,"end":923},"obj":"Species:9606"},{"id":"T2","span":{"begin":93,"end":102},"obj":"Species:9606"},{"id":"T3","span":{"begin":110,"end":114},"obj":"Gene:10927"},{"id":"T4","span":{"begin":188,"end":197},"obj":"Species:9606"},{"id":"T5","span":{"begin":272,"end":276},"obj":"Gene:10927"},{"id":"T6","span":{"begin":387,"end":391},"obj":"Gene:10927"},{"id":"T7","span":{"begin":457,"end":466},"obj":"Species:9606"},{"id":"T8","span":{"begin":501,"end":512},"obj":"Species:7091"},{"id":"T9","span":{"begin":865,"end":869},"obj":"Gene:10927"}],"text":"Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells.\nSpin-filters have been primarily used for producing therapeutic proteins from mammalian cells. However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods."}

{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":110,"end":204},"obj":"DRI_Background"},{"id":"T2","span":{"begin":205,"end":336},"obj":"DRI_Background"},{"id":"T3","span":{"begin":337,"end":486},"obj":"DRI_Background"},{"id":"T4","span":{"begin":487,"end":637},"obj":"DRI_Background"},{"id":"T5","span":{"begin":638,"end":782},"obj":"DRI_Approach"},{"id":"T6","span":{"begin":783,"end":955},"obj":"DRI_Outcome"},{"id":"T7","span":{"begin":956,"end":1085},"obj":"DRI_Background"}],"text":"Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells.\nSpin-filters have been primarily used for producing therapeutic proteins from mammalian cells. However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":23,"end":27},"obj":"Gene:10927"},{"id":"T2","span":{"begin":93,"end":102},"obj":"Species:9606"},{"id":"T3","span":{"begin":110,"end":114},"obj":"Gene:10927"},{"id":"T4","span":{"begin":188,"end":197},"obj":"Species:9606"},{"id":"T5","span":{"begin":272,"end":276},"obj":"Gene:10927"},{"id":"T6","span":{"begin":387,"end":391},"obj":"Gene:10927"},{"id":"T7","span":{"begin":457,"end":466},"obj":"Species:9606"},{"id":"T8","span":{"begin":501,"end":512},"obj":"Species:7091"},{"id":"T9","span":{"begin":865,"end":869},"obj":"Gene:10927"},{"id":"T10","span":{"begin":918,"end":923},"obj":"Species:9606"}],"text":"Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells.\nSpin-filters have been primarily used for producing therapeutic proteins from mammalian cells. However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods."}