| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
110-204 |
DRI_Background |
denotes |
Spin-filters have been primarily used for producing therapeutic proteins from mammalian cells. |
| T2 |
205-336 |
DRI_Background |
denotes |
However, disposability and/or high filter clogging of the existing spin-filter systems affect the process economy and productivity. |
| T3 |
337-486 |
DRI_Background |
denotes |
Hence, to address these drawbacks a reusable dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells was designed. |
| T4 |
487-637 |
DRI_Background |
denotes |
Two non-woven Bombyx mori silk layers were used as filter screen; the outer layer was conducive to cell attachment whilst the inner was non-conducive. |
| T5 |
638-782 |
DRI_Approach |
denotes |
Adherent cells can be cultured either in suspended mode using its inner single module or as monolayer of cells using its dual concentric module. |
| T6 |
783-955 |
DRI_Outcome |
denotes |
We achieved 30 % higher urokinase productivity as compared to the stainless-steel spin-filter during perfusion experiments of adherent human kidney cells in suspended mode. |
| T7 |
956-1085 |
DRI_Background |
denotes |
This was due to the hydrophobic and negatively-charged silk screen that allows clog-free perfusion culture for prolonged periods. |
