PubMed:24581344 / 739-1135 JSONTXT

{"project":"PubMed_Structured_Abstracts","denotations":[{"id":"T2","span":{"begin":0,"end":396},"obj":"METHODS"}],"text":"A \"top-down\" approach was used to characterize changes in VIC phenotype in response to collagen alterations in the native 3D environment. Collagen-deficient leaflets were created via enzymatic treatment and cultured statically for six days in vitro. After culture, leaflets were harvested for analysis of DNA, proliferation, apoptosis, ECM composition, calcification, and gene/protein expression."}

{"project":"Goldhamster2_Cellosaurus","denotations":[{"id":"T4","span":{"begin":0,"end":1},"obj":"CVCL_6479|Finite_cell_line|Mus musculus"},{"id":"T5","span":{"begin":47,"end":54},"obj":"CVCL_0238|Cancer_cell_line|Homo sapiens"}],"text":"A \"top-down\" approach was used to characterize changes in VIC phenotype in response to collagen alterations in the native 3D environment. Collagen-deficient leaflets were created via enzymatic treatment and cultured statically for six days in vitro. After culture, leaflets were harvested for analysis of DNA, proliferation, apoptosis, ECM composition, calcification, and gene/protein expression."}

{"project":"GoldHamster","denotations":[{"id":"T45","span":{"begin":58,"end":61},"obj":"PR:000006898"},{"id":"T46","span":{"begin":58,"end":61},"obj":"P23943"},{"id":"T47","span":{"begin":58,"end":61},"obj":"P22389"},{"id":"T49","span":{"begin":87,"end":95},"obj":"2714"},{"id":"T50","span":{"begin":87,"end":95},"obj":"D003094"},{"id":"T51","span":{"begin":87,"end":95},"obj":"CHEBI:3815"},{"id":"T48","span":{"begin":87,"end":95},"obj":"GO:0005202"},{"id":"T53","span":{"begin":138,"end":146},"obj":"2714"},{"id":"T54","span":{"begin":138,"end":146},"obj":"D003094"},{"id":"T55","span":{"begin":138,"end":146},"obj":"CHEBI:3815"},{"id":"T52","span":{"begin":138,"end":146},"obj":"GO:0005202"},{"id":"T56","span":{"begin":183,"end":192},"obj":"SO:0001185"},{"id":"T59","span":{"begin":305,"end":308},"obj":"SO:0000352"},{"id":"T57","span":{"begin":305,"end":308},"obj":"GO:0005574"},{"id":"T58","span":{"begin":305,"end":308},"obj":"CHEBI:16991"},{"id":"T60","span":{"begin":305,"end":308},"obj":"D004247"},{"id":"T61","span":{"begin":310,"end":323},"obj":"UBERON:2000098"},{"id":"T62","span":{"begin":325,"end":334},"obj":"GO:0097194"},{"id":"T63","span":{"begin":325,"end":334},"obj":"GO:0006915"},{"id":"T64","span":{"begin":336,"end":339},"obj":"PR:Q13201"},{"id":"T66","span":{"begin":336,"end":339},"obj":"PR:000010492"},{"id":"T65","span":{"begin":336,"end":339},"obj":"UBERON:4000022"},{"id":"T67","span":{"begin":372,"end":376},"obj":"SO:0000704"},{"id":"T72","span":{"begin":377,"end":384},"obj":"SO:0000104"},{"id":"T71","span":{"begin":377,"end":384},"obj":"PR:000000001"},{"id":"T68","span":{"begin":377,"end":384},"obj":"GO:0003675"},{"id":"T69","span":{"begin":377,"end":384},"obj":"CHEBI:36080"},{"id":"T70","span":{"begin":377,"end":384},"obj":"CHEBI:11122"}],"text":"A \"top-down\" approach was used to characterize changes in VIC phenotype in response to collagen alterations in the native 3D environment. Collagen-deficient leaflets were created via enzymatic treatment and cultured statically for six days in vitro. After culture, leaflets were harvested for analysis of DNA, proliferation, apoptosis, ECM composition, calcification, and gene/protein expression."}