PubMed:24192013 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/24192013","sourcedb":"PubMed","sourceid":"24192013","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/24192013","text":"Differential expression of CPKs and cytosolic Ca2+ variation in resistant and susceptible apple cultivars (Malus x domestica) in response to the pathogen Erwinia amylovora and mechanical wounding.\nBACKGROUND: Plant calcium (Ca2+) signals are involved in a wide array of intracellular signalling pathways following pathogen invasion. Ca2+-binding sensory proteins such as Ca2+-dependent protein kinases (CPKs) have been predicted to mediate signalling following Ca2+ influx after pathogen infection. However, to date this prediction has remained elusive.\nRESULTS: We conducted a genome-wide identification of the Malus x domestica CPK (MdCPK) gene family and identified 30 CPK genes. Comparative phylogenetic analysis of Malus CPKs with CPKs of Arabidopsis thaliana (AtCPKs), Oryza sativa (OsCPKs), Populous trichocarpa (PtCPKs) and Zea mays (ZmCPKs) revealed four different groups. From the phylogenetic tree, we found that MdCPKs are closely related to AtCPKs and PtCPKs rather than OsCPKs and ZmCPKs, indicating their dicot-specific origin. Furthermore, comparative quantitative real time PCR and intracellular cytosolic calcium ([Ca2+]cyt) analysis were carried out on fire blight resistant and susceptible M. x domestica apple cultivars following infection with a pathogen (Erwinia amylovora) and/or mechanical damage. Calcium analysis showed an increased [Ca2+]cyt over time in resistant cultivars as compared to susceptible cultivars. Gene expression studies showed that 11 out of the 30 MdCPKs were differentially expressed following pathogen infection.\nCONCLUSIONS: We studied the genome-wide analysis of MdCPK gene family in Malus x domestica and analyzed their differential gene expression along with cytosolic calcium variation upon pathogen infection. There was a striking difference in MdCPKs gene expressions and [Ca2+]cyt variations between resistant and susceptible M. x domestica cultivars in response to E. amylovora and mechanical wounding. Our genomic and bioinformatic analysis provided an important insight about the role of MdCPKs in modulating defence responses in susceptible and resistant apple cultivars. It also provided further information on early signalling and downstream signalling cascades in response to pathogenic and mechanical stress.","tracks":[{"project":"OryzaGP_2021_FLAIR","denotations":[{"id":"M_0","span":{"begin":775,"end":787},"obj":"hunflair:NA:Species"},{"id":"M_1","span":{"begin":766,"end":772},"obj":"hunflair:NA:Gene"},{"id":"M_2","span":{"begin":954,"end":960},"obj":"hunflair:NA:Gene"},{"id":"M_3","span":{"begin":789,"end":795},"obj":"hunflair:NA:Gene"},{"id":"M_4","span":{"begin":984,"end":990},"obj":"hunflair:NA:Gene"},{"id":"M_5","span":{"begin":1613,"end":1630},"obj":"hunflair:NA:Gene"},{"id":"M_6","span":{"begin":107,"end":124},"obj":"hunflair:NA:Species"},{"id":"M_7","span":{"begin":612,"end":629},"obj":"hunflair:NA:Species"},{"id":"M_8","span":{"begin":1634,"end":1651},"obj":"hunflair:NA:Species"},{"id":"M_9","span":{"begin":744,"end":764},"obj":"hunflair:NA:Species"},{"id":"M_10","span":{"begin":371,"end":401},"obj":"hunflair:NA:Gene"},{"id":"M_11","span":{"begin":820,"end":826},"obj":"hunflair:NA:Gene"},{"id":"M_12","span":{"begin":965,"end":971},"obj":"hunflair:NA:Gene"},{"id":"M_13","span":{"begin":1323,"end":1330},"obj":"hunflair:NA:Chemical"},{"id":"M_14","span":{"begin":924,"end":930},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":1494,"end":1500},"obj":"hunflair:NA:Gene"},{"id":"M_16","span":{"begin":1799,"end":1805},"obj":"hunflair:NA:Gene"},{"id":"M_17","span":{"begin":2047,"end":2053},"obj":"hunflair:NA:Gene"},{"id":"M_18","span":{"begin":215,"end":222},"obj":"hunflair:NA:Chemical"},{"id":"M_19","span":{"begin":1123,"end":1130},"obj":"hunflair:NA:Chemical"},{"id":"M_20","span":{"begin":1721,"end":1728},"obj":"hunflair:NA:Chemical"},{"id":"M_21","span":{"begin":1922,"end":1934},"obj":"hunflair:NA:Species"},{"id":"M_22","span":{"begin":479,"end":497},"obj":"hunflair:NA:Disease"},{"id":"M_23","span":{"begin":1541,"end":1559},"obj":"hunflair:NA:Disease"},{"id":"M_24","span":{"begin":1744,"end":1762},"obj":"hunflair:NA:Disease"},{"id":"M_25","span":{"begin":720,"end":730},"obj":"hunflair:NA:Gene"},{"id":"M_26","span":{"begin":842,"end":848},"obj":"hunflair:NA:Gene"},{"id":"M_27","span":{"begin":995,"end":1001},"obj":"hunflair:NA:Gene"},{"id":"M_28","span":{"begin":798,"end":818},"obj":"hunflair:NA:Species"},{"id":"M_29","span":{"begin":630,"end":633},"obj":"hunflair:NA:Gene"},{"id":"M_30","span":{"begin":672,"end":675},"obj":"hunflair:NA:Gene"},{"id":"M_31","span":{"begin":27,"end":31},"obj":"hunflair:NA:Gene"},{"id":"M_32","span":{"begin":403,"end":407},"obj":"hunflair:NA:Gene"},{"id":"M_33","span":{"begin":726,"end":730},"obj":"hunflair:NA:Gene"},{"id":"M_34","span":{"begin":736,"end":740},"obj":"hunflair:NA:Gene"},{"id":"M_35","span":{"begin":90,"end":95},"obj":"hunflair:NA:Species"},{"id":"M_36","span":{"begin":1225,"end":1230},"obj":"hunflair:NA:Species"},{"id":"M_37","span":{"begin":2115,"end":2120},"obj":"hunflair:NA:Species"},{"id":"M_38","span":{"begin":832,"end":840},"obj":"hunflair:NA:Species"},{"id":"M_39","span":{"begin":154,"end":171},"obj":"hunflair:NA:Species"},{"id":"M_40","span":{"begin":1278,"end":1295},"obj":"hunflair:NA:Species"},{"id":"M_41","span":{"begin":46,"end":49},"obj":"hunflair:NA:Chemical"},{"id":"M_42","span":{"begin":224,"end":227},"obj":"hunflair:NA:Chemical"},{"id":"M_43","span":{"begin":333,"end":336},"obj":"hunflair:NA:Chemical"},{"id":"M_44","span":{"begin":371,"end":374},"obj":"hunflair:NA:Chemical"},{"id":"M_45","span":{"begin":461,"end":464},"obj":"hunflair:NA:Chemical"},{"id":"M_46","span":{"begin":1133,"end":1136},"obj":"hunflair:NA:Chemical"},{"id":"M_47","span":{"begin":1361,"end":1364},"obj":"hunflair:NA:Chemical"},{"id":"M_48","span":{"begin":1828,"end":1831},"obj":"hunflair:NA:Chemical"},{"id":"M_49","span":{"begin":1210,"end":1224},"obj":"hunflair:NA:Species"},{"id":"M_50","span":{"begin":1882,"end":1896},"obj":"hunflair:NA:Species"}],"attributes":[{"subj":"M_0","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_1","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_2","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_3","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_4","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_5","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_6","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_7","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_8","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_9","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_10","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_11","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_12","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_13","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_14","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_15","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_16","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_17","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_18","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_19","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_20","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_21","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_22","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_23","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_24","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_25","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_26","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_27","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_28","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_29","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_30","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_31","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_32","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_33","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_34","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_35","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_36","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_37","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_38","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_39","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_40","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_41","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_42","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_43","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_44","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_45","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_46","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_47","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_48","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_49","pred":"source","obj":"OryzaGP_2021_FLAIR"},{"subj":"M_50","pred":"source","obj":"OryzaGP_2021_FLAIR"}]},{"project":"OryzaGP_2021","denotations":[{"id":"T1","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/9457"},{"id":"T2","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/7167"},{"id":"T3","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/7166"},{"id":"T4","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/6431"},{"id":"T5","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/456"},{"id":"T6","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/17646"},{"id":"T7","span":{"begin":36,"end":45},"obj":"http://identifiers.org/oryzabase.gene/14451"},{"id":"T8","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/9457"},{"id":"T9","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/7167"},{"id":"T10","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/7166"},{"id":"T11","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/6431"},{"id":"T12","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/456"},{"id":"T13","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/17646"},{"id":"T14","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/oryzabase.gene/14451"},{"id":"T15","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/9457"},{"id":"T16","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/7167"},{"id":"T17","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/7166"},{"id":"T18","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/6431"},{"id":"T19","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/456"},{"id":"T20","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/17646"},{"id":"T21","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/oryzabase.gene/14451"},{"id":"T9241","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os07g49400"},{"id":"T65349","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os07g46990"},{"id":"T35436","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os06g45710"},{"id":"T73344","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os03g22810"},{"id":"T15556","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os03g17690"},{"id":"T57507","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os02g07260"},{"id":"T54926","span":{"begin":36,"end":45},"obj":"http://identifiers.org/ricegap/LOC_Os01g05490"},{"id":"T48136","span":{"begin":386,"end":401},"obj":"http://identifiers.org/ricegap/LOC_Os07g04820"},{"id":"T57769","span":{"begin":386,"end":401},"obj":"http://identifiers.org/ricegap/LOC_Os06g18000"},{"id":"T62667","span":{"begin":386,"end":401},"obj":"http://identifiers.org/ricegap/LOC_Os06g13320"},{"id":"T65311","span":{"begin":386,"end":401},"obj":"http://identifiers.org/ricegap/LOC_Os01g02830"},{"id":"T5352","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os07g49400"},{"id":"T77461","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os07g46990"},{"id":"T56950","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os06g45710"},{"id":"T36783","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os03g22810"},{"id":"T12713","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os03g17690"},{"id":"T40239","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os02g07260"},{"id":"T7232","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/ricegap/LOC_Os01g05490"},{"id":"T12429","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os07g49400"},{"id":"T54153","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os07g46990"},{"id":"T84533","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os06g45710"},{"id":"T22","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os03g22810"},{"id":"T23","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os03g17690"},{"id":"T24","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os02g07260"},{"id":"T25","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/ricegap/LOC_Os01g05490"},{"id":"T93537","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os07g0694700"},{"id":"T7613","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os07g0665200"},{"id":"T32205","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os06g0668200"},{"id":"T14876","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os03g0351500"},{"id":"T47533","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os03g0285700"},{"id":"T2557","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os02g0169300"},{"id":"T56128","span":{"begin":36,"end":45},"obj":"http://identifiers.org/rapdb.locus/Os01g0147900"},{"id":"T66806","span":{"begin":386,"end":401},"obj":"http://identifiers.org/rapdb.locus/Os06g0288100"},{"id":"T49708","span":{"begin":386,"end":401},"obj":"http://identifiers.org/rapdb.locus/Os06g0241100"},{"id":"T76001","span":{"begin":386,"end":401},"obj":"http://identifiers.org/rapdb.locus/Os01g0117600"},{"id":"T74341","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os07g0694700"},{"id":"T68427","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os07g0665200"},{"id":"T3071","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os06g0668200"},{"id":"T89331","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os03g0351500"},{"id":"T56897","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os03g0285700"},{"id":"T4460","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os02g0169300"},{"id":"T73598","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/rapdb.locus/Os01g0147900"},{"id":"T91631","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os07g0694700"},{"id":"T63663","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os07g0665200"},{"id":"T3333","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os06g0668200"},{"id":"T33660","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os03g0351500"},{"id":"T21256","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os03g0285700"},{"id":"T96179","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os02g0169300"},{"id":"T54069","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/rapdb.locus/Os01g0147900"},{"id":"T23719","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/Q9FE01"},{"id":"T81049","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/Q10N21"},{"id":"T49419","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/Q0DRV6"},{"id":"T74861","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/P48494"},{"id":"T60808","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/P28757"},{"id":"T13312","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/B8AIH2"},{"id":"T73214","span":{"begin":36,"end":45},"obj":"http://identifiers.org/uniprot/A2YG06"},{"id":"T83982","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/Q9FE01"},{"id":"T98757","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/Q10N21"},{"id":"T13113","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/Q0DRV6"},{"id":"T51666","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/P48494"},{"id":"T16552","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/P28757"},{"id":"T69588","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/B8AIH2"},{"id":"T41999","span":{"begin":1113,"end":1122},"obj":"http://identifiers.org/uniprot/A2YG06"},{"id":"T76621","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/Q9FE01"},{"id":"T11656","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/Q10N21"},{"id":"T27688","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/Q0DRV6"},{"id":"T18293","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/P48494"},{"id":"T64084","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/P28757"},{"id":"T86801","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/B8AIH2"},{"id":"T25690","span":{"begin":1711,"end":1720},"obj":"http://identifiers.org/uniprot/A2YG06"},{"id":"M_0","span":{"begin":775,"end":787},"obj":"hunflair:NA:Species"},{"id":"M_1","span":{"begin":766,"end":772},"obj":"hunflair:NA:Gene"},{"id":"M_2","span":{"begin":954,"end":960},"obj":"hunflair:NA:Gene"},{"id":"M_3","span":{"begin":789,"end":795},"obj":"hunflair:NA:Gene"},{"id":"M_4","span":{"begin":984,"end":990},"obj":"hunflair:NA:Gene"},{"id":"M_5","span":{"begin":1613,"end":1630},"obj":"hunflair:NA:Gene"},{"id":"M_6","span":{"begin":107,"end":124},"obj":"hunflair:NA:Species"},{"id":"M_7","span":{"begin":612,"end":629},"obj":"hunflair:NA:Species"},{"id":"M_8","span":{"begin":1634,"end":1651},"obj":"hunflair:NA:Species"},{"id":"M_9","span":{"begin":744,"end":764},"obj":"hunflair:NA:Species"},{"id":"M_10","span":{"begin":371,"end":401},"obj":"hunflair:NA:Gene"},{"id":"M_11","span":{"begin":820,"end":826},"obj":"hunflair:NA:Gene"},{"id":"M_12","span":{"begin":965,"end":971},"obj":"hunflair:NA:Gene"},{"id":"M_13","span":{"begin":1323,"end":1330},"obj":"hunflair:NA:Chemical"},{"id":"M_14","span":{"begin":924,"end":930},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":1494,"end":1500},"obj":"hunflair:NA:Gene"},{"id":"M_16","span":{"begin":1799,"end":1805},"obj":"hunflair:NA:Gene"},{"id":"M_17","span":{"begin":2047,"end":2053},"obj":"hunflair:NA:Gene"},{"id":"M_18","span":{"begin":215,"end":222},"obj":"hunflair:NA:Chemical"},{"id":"M_19","span":{"begin":1123,"end":1130},"obj":"hunflair:NA:Chemical"},{"id":"M_20","span":{"begin":1721,"end":1728},"obj":"hunflair:NA:Chemical"},{"id":"M_21","span":{"begin":1922,"end":1934},"obj":"hunflair:NA:Species"},{"id":"M_22","span":{"begin":479,"end":497},"obj":"hunflair:NA:Disease"},{"id":"M_23","span":{"begin":1541,"end":1559},"obj":"hunflair:NA:Disease"},{"id":"M_24","span":{"begin":1744,"end":1762},"obj":"hunflair:NA:Disease"},{"id":"M_25","span":{"begin":720,"end":730},"obj":"hunflair:NA:Gene"},{"id":"M_26","span":{"begin":842,"end":848},"obj":"hunflair:NA:Gene"},{"id":"M_27","span":{"begin":995,"end":1001},"obj":"hunflair:NA:Gene"},{"id":"M_28","span":{"begin":798,"end":818},"obj":"hunflair:NA:Species"},{"id":"M_29","span":{"begin":630,"end":633},"obj":"hunflair:NA:Gene"},{"id":"M_30","span":{"begin":672,"end":675},"obj":"hunflair:NA:Gene"},{"id":"M_31","span":{"begin":27,"end":31},"obj":"hunflair:NA:Gene"},{"id":"M_32","span":{"begin":403,"end":407},"obj":"hunflair:NA:Gene"},{"id":"M_33","span":{"begin":726,"end":730},"obj":"hunflair:NA:Gene"},{"id":"M_34","span":{"begin":736,"end":740},"obj":"hunflair:NA:Gene"},{"id":"M_35","span":{"begin":90,"end":95},"obj":"hunflair:NA:Species"},{"id":"M_36","span":{"begin":1225,"end":1230},"obj":"hunflair:NA:Species"},{"id":"M_37","span":{"begin":2115,"end":2120},"obj":"hunflair:NA:Species"},{"id":"M_38","span":{"begin":832,"end":840},"obj":"hunflair:NA:Species"},{"id":"M_39","span":{"begin":154,"end":171},"obj":"hunflair:NA:Species"},{"id":"M_40","span":{"begin":1278,"end":1295},"obj":"hunflair:NA:Species"},{"id":"M_41","span":{"begin":46,"end":49},"obj":"hunflair:NA:Chemical"},{"id":"M_42","span":{"begin":224,"end":227},"obj":"hunflair:NA:Chemical"},{"id":"M_43","span":{"begin":333,"end":336},"obj":"hunflair:NA:Chemical"},{"id":"M_44","span":{"begin":371,"end":374},"obj":"hunflair:NA:Chemical"},{"id":"M_45","span":{"begin":461,"end":464},"obj":"hunflair:NA:Chemical"},{"id":"M_46","span":{"begin":1133,"end":1136},"obj":"hunflair:NA:Chemical"},{"id":"M_47","span":{"begin":1361,"end":1364},"obj":"hunflair:NA:Chemical"},{"id":"M_48","span":{"begin":1828,"end":1831},"obj":"hunflair:NA:Chemical"},{"id":"M_49","span":{"begin":1210,"end":1224},"obj":"hunflair:NA:Species"},{"id":"M_50","span":{"begin":1882,"end":1896},"obj":"hunflair:NA:Species"}],"attributes":[{"subj":"T1","pred":"source","obj":"OryzaGP_2021"},{"subj":"T2","pred":"source","obj":"OryzaGP_2021"},{"subj":"T3","pred":"source","obj":"OryzaGP_2021"},{"subj":"T4","pred":"source","obj":"OryzaGP_2021"},{"subj":"T5","pred":"source","obj":"OryzaGP_2021"},{"subj":"T6","pred":"source","obj":"OryzaGP_2021"},{"subj":"T7","pred":"source","obj":"OryzaGP_2021"},{"subj":"T8","pred":"source","obj":"OryzaGP_2021"},{"subj":"T9","pred":"source","obj":"OryzaGP_2021"},{"subj":"T10","pred":"source","obj":"OryzaGP_2021"},{"subj":"T11","pred":"source","obj":"OryzaGP_2021"},{"subj":"T12","pred":"source","obj":"OryzaGP_2021"},{"subj":"T13","pred":"source","obj":"OryzaGP_2021"},{"subj":"T14","pred":"source","obj":"OryzaGP_2021"},{"subj":"T15","pred":"source","obj":"OryzaGP_2021"},{"subj":"T16","pred":"source","obj":"OryzaGP_2021"},{"subj":"T17","pred":"source","obj":"OryzaGP_2021"},{"subj":"T18","pred":"source","obj":"OryzaGP_2021"},{"subj":"T19","pred":"source","obj":"OryzaGP_2021"},{"subj":"T20","pred":"source","obj":"OryzaGP_2021"},{"subj":"T21","pred":"source","obj":"OryzaGP_2021"},{"subj":"T9241","pred":"source","obj":"OryzaGP_2021"},{"subj":"T65349","pred":"source","obj":"OryzaGP_2021"},{"subj":"T35436","pred":"source","obj":"OryzaGP_2021"},{"subj":"T73344","pred":"source","obj":"OryzaGP_2021"},{"subj":"T15556","pred":"source","obj":"OryzaGP_2021"},{"subj":"T57507","pred":"source","obj":"OryzaGP_2021"},{"subj":"T54926","pred":"source","obj":"OryzaGP_2021"},{"subj":"T48136","pred":"source","obj":"OryzaGP_2021"},{"subj":"T57769","pred":"source","obj":"OryzaGP_2021"},{"subj":"T62667","pred":"source","obj":"OryzaGP_2021"},{"subj":"T65311","pred":"source","obj":"OryzaGP_2021"},{"subj":"T5352","pred":"source","obj":"OryzaGP_2021"},{"subj":"T77461","pred":"source","obj":"OryzaGP_2021"},{"subj":"T56950","pred":"source","obj":"OryzaGP_2021"},{"subj":"T36783","pred":"source","obj":"OryzaGP_2021"},{"subj":"T12713","pred":"source","obj":"OryzaGP_2021"},{"subj":"T40239","pred":"source","obj":"OryzaGP_2021"},{"subj":"T7232","pred":"source","obj":"OryzaGP_2021"},{"subj":"T12429","pred":"source","obj":"OryzaGP_2021"},{"subj":"T54153","pred":"source","obj":"OryzaGP_2021"},{"subj":"T84533","pred":"source","obj":"OryzaGP_2021"},{"subj":"T22","pred":"source","obj":"OryzaGP_2021"},{"subj":"T23","pred":"source","obj":"OryzaGP_2021"},{"subj":"T24","pred":"source","obj":"OryzaGP_2021"},{"subj":"T25","pred":"source","obj":"OryzaGP_2021"},{"subj":"T93537","pred":"source","obj":"OryzaGP_2021"},{"subj":"T7613","pred":"source","obj":"OryzaGP_2021"},{"subj":"T32205","pred":"source","obj":"OryzaGP_2021"},{"subj":"T14876","pred":"source","obj":"OryzaGP_2021"},{"subj":"T47533","pred":"source","obj":"OryzaGP_2021"},{"subj":"T2557","pred":"source","obj":"OryzaGP_2021"},{"subj":"T56128","pred":"source","obj":"OryzaGP_2021"},{"subj":"T66806","pred":"source","obj":"OryzaGP_2021"},{"subj":"T49708","pred":"source","obj":"OryzaGP_2021"},{"subj":"T76001","pred":"source","obj":"OryzaGP_2021"},{"subj":"T74341","pred":"source","obj":"OryzaGP_2021"},{"subj":"T68427","pred":"source","obj":"OryzaGP_2021"},{"subj":"T3071","pred":"source","obj":"OryzaGP_2021"},{"subj":"T89331","pred":"source","obj":"OryzaGP_2021"},{"subj":"T56897","pred":"source","obj":"OryzaGP_2021"},{"subj":"T4460","pred":"source","obj":"OryzaGP_2021"},{"subj":"T73598","pred":"source","obj":"OryzaGP_2021"},{"subj":"T91631","pred":"source","obj":"OryzaGP_2021"},{"subj":"T63663","pred":"source","obj":"OryzaGP_2021"},{"subj":"T3333","pred":"source","obj":"OryzaGP_2021"},{"subj":"T33660","pred":"source","obj":"OryzaGP_2021"},{"subj":"T21256","pred":"source","obj":"OryzaGP_2021"},{"subj":"T96179","pred":"source","obj":"OryzaGP_2021"},{"subj":"T54069","pred":"source","obj":"OryzaGP_2021"},{"subj":"T23719","pred":"source","obj":"OryzaGP_2021"},{"subj":"T81049","pred":"source","obj":"OryzaGP_2021"},{"subj":"T49419","pred":"source","obj":"OryzaGP_2021"},{"subj":"T74861","pred":"source","obj":"OryzaGP_2021"},{"subj":"T60808","pred":"source","obj":"OryzaGP_2021"},{"subj":"T13312","pred":"source","obj":"OryzaGP_2021"},{"subj":"T73214","pred":"source","obj":"OryzaGP_2021"},{"subj":"T83982","pred":"source","obj":"OryzaGP_2021"},{"subj":"T98757","pred":"source","obj":"OryzaGP_2021"},{"subj":"T13113","pred":"source","obj":"OryzaGP_2021"},{"subj":"T51666","pred":"source","obj":"OryzaGP_2021"},{"subj":"T16552","pred":"source","obj":"OryzaGP_2021"},{"subj":"T69588","pred":"source","obj":"OryzaGP_2021"},{"subj":"T41999","pred":"source","obj":"OryzaGP_2021"},{"subj":"T76621","pred":"source","obj":"OryzaGP_2021"},{"subj":"T11656","pred":"source","obj":"OryzaGP_2021"},{"subj":"T27688","pred":"source","obj":"OryzaGP_2021"},{"subj":"T18293","pred":"source","obj":"OryzaGP_2021"},{"subj":"T64084","pred":"source","obj":"OryzaGP_2021"},{"subj":"T86801","pred":"source","obj":"OryzaGP_2021"},{"subj":"T25690","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_0","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_1","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_2","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_3","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_4","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_5","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_6","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_7","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_8","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_9","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_10","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_11","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_12","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_13","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_14","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_15","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_16","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_17","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_18","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_19","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_20","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_21","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_22","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_23","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_24","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_25","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_26","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_27","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_28","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_29","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_30","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_31","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_32","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_33","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_34","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_35","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_36","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_37","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_38","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_39","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_40","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_41","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_42","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_43","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_44","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_45","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_46","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_47","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_48","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_49","pred":"source","obj":"OryzaGP_2021"},{"subj":"M_50","pred":"source","obj":"OryzaGP_2021"}]},{"project":"Allie","denotations":[{"id":"SS1_24192013_6_0","span":{"begin":612,"end":633},"obj":"expanded"},{"id":"SS2_24192013_6_0","span":{"begin":635,"end":640},"obj":"abbr"}],"relations":[{"id":"AE1_24192013_6_0","pred":"abbreviatedTo","subj":"SS1_24192013_6_0","obj":"SS2_24192013_6_0"}],"attributes":[{"subj":"SS1_24192013_6_0","pred":"source","obj":"Allie"},{"subj":"SS2_24192013_6_0","pred":"source","obj":"Allie"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"OryzaGP_2021_FLAIR","color":"#c293ec","default":true},{"id":"OryzaGP_2021","color":"#93eca8"},{"id":"Allie","color":"#ec9397"}]}]}}