PubMed:237552 JSONTXT

{"project":"Test-Species-PubTator","denotations":[{"id":"4","span":{"begin":16,"end":33},"obj":"Chemical"},{"id":"5","span":{"begin":38,"end":56},"obj":"Chemical"},{"id":"6","span":{"begin":57,"end":75},"obj":"Gene"},{"id":"7","span":{"begin":81,"end":87},"obj":"Species"},{"id":"35","span":{"begin":105,"end":123},"obj":"Gene"},{"id":"36","span":{"begin":127,"end":133},"obj":"Species"},{"id":"37","span":{"begin":193,"end":210},"obj":"Chemical"},{"id":"38","span":{"begin":308,"end":316},"obj":"Chemical"},{"id":"39","span":{"begin":318,"end":323},"obj":"Chemical"},{"id":"40","span":{"begin":330,"end":351},"obj":"Species"},{"id":"41","span":{"begin":428,"end":436},"obj":"Chemical"},{"id":"42","span":{"begin":438,"end":455},"obj":"Chemical"},{"id":"43","span":{"begin":461,"end":479},"obj":"Chemical"},{"id":"44","span":{"begin":512,"end":520},"obj":"Chemical"},{"id":"45","span":{"begin":522,"end":533},"obj":"Chemical"},{"id":"46","span":{"begin":540,"end":556},"obj":"Chemical"},{"id":"47","span":{"begin":569,"end":577},"obj":"Chemical"},{"id":"48","span":{"begin":579,"end":586},"obj":"Chemical"},{"id":"49","span":{"begin":641,"end":648},"obj":"Chemical"},{"id":"50","span":{"begin":653,"end":662},"obj":"Chemical"},{"id":"51","span":{"begin":699,"end":713},"obj":"Chemical"},{"id":"52","span":{"begin":850,"end":867},"obj":"Chemical"},{"id":"53","span":{"begin":872,"end":883},"obj":"Chemical"},{"id":"54","span":{"begin":887,"end":901},"obj":"Chemical"},{"id":"55","span":{"begin":946,"end":951},"obj":"Chemical"},{"id":"56","span":{"begin":978,"end":985},"obj":"Chemical"},{"id":"57","span":{"begin":1030,"end":1041},"obj":"Chemical"},{"id":"58","span":{"begin":1165,"end":1189},"obj":"Chemical"},{"id":"59","span":{"begin":1191,"end":1202},"obj":"Chemical"},{"id":"60","span":{"begin":1207,"end":1214},"obj":"Chemical"},{"id":"61","span":{"begin":1256,"end":1274},"obj":"Gene"}],"attributes":[{"id":"A4","pred":"resolved_to","subj":"4","obj":"MESH:D005677"},{"id":"A5","pred":"resolved_to","subj":"5","obj":"MESH:C009744"},{"id":"A6","pred":"resolved_to","subj":"6","obj":"2720"},{"id":"A7","pred":"resolved_to","subj":"7","obj":"9986"},{"id":"A35","pred":"resolved_to","subj":"35","obj":"2720"},{"id":"A36","pred":"resolved_to","subj":"36","obj":"9986"},{"id":"A37","pred":"resolved_to","subj":"37","obj":"MESH:D005677"},{"id":"A38","pred":"resolved_to","subj":"38","obj":"MESH:D002518"},{"id":"A39","pred":"resolved_to","subj":"39","obj":"MESH:D005677"},{"id":"A41","pred":"resolved_to","subj":"41","obj":"MESH:D002518"},{"id":"A42","pred":"resolved_to","subj":"42","obj":"MESH:D005678"},{"id":"A43","pred":"resolved_to","subj":"43","obj":"MESH:C009744"},{"id":"A44","pred":"resolved_to","subj":"44","obj":"MESH:D002518"},{"id":"A45","pred":"resolved_to","subj":"45","obj":"-"},{"id":"A46","pred":"resolved_to","subj":"46","obj":"MESH:D005963"},{"id":"A47","pred":"resolved_to","subj":"47","obj":"MESH:D002518"},{"id":"A48","pred":"resolved_to","subj":"48","obj":"-"},{"id":"A49","pred":"resolved_to","subj":"49","obj":"-"},{"id":"A50","pred":"resolved_to","subj":"50","obj":"-"},{"id":"A51","pred":"resolved_to","subj":"51","obj":"MESH:C025614"},{"id":"A52","pred":"resolved_to","subj":"52","obj":"MESH:D005677"},{"id":"A53","pred":"resolved_to","subj":"53","obj":"-"},{"id":"A54","pred":"resolved_to","subj":"54","obj":"MESH:C016679"},{"id":"A55","pred":"resolved_to","subj":"55","obj":"MESH:D005677"},{"id":"A56","pred":"resolved_to","subj":"56","obj":"MESH:D000085"},{"id":"A57","pred":"resolved_to","subj":"57","obj":"-"},{"id":"A58","pred":"resolved_to","subj":"58","obj":"-"},{"id":"A59","pred":"resolved_to","subj":"59","obj":"MESH:D005690"},{"id":"A60","pred":"resolved_to","subj":"60","obj":"MESH:D007785"},{"id":"A61","pred":"resolved_to","subj":"61","obj":"2720"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"Test-Species-PubDictionaries-PubMedBERT","denotations":[{"id":"T1","span":{"begin":22,"end":33},"obj":"Species"},{"id":"T2","span":{"begin":81,"end":87},"obj":"Species"},{"id":"T3","span":{"begin":99,"end":104},"obj":"Species"},{"id":"T4","span":{"begin":127,"end":133},"obj":"Species"},{"id":"T5","span":{"begin":199,"end":210},"obj":"Species"},{"id":"T6","span":{"begin":330,"end":339},"obj":"Species"},{"id":"T7","span":{"begin":340,"end":351},"obj":"Species"},{"id":"T8","span":{"begin":444,"end":455},"obj":"Species"},{"id":"T9","span":{"begin":600,"end":604},"obj":"Species"},{"id":"T10","span":{"begin":856,"end":867},"obj":"Species"},{"id":"T11","span":{"begin":1220,"end":1224},"obj":"Species"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"2904946"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9986"},{"id":"A3","pred":"db_id","subj":"T3","obj":"1925465"},{"id":"A4","pred":"db_id","subj":"T4","obj":"9986"},{"id":"A5","pred":"db_id","subj":"T5","obj":"2904946"},{"id":"A6","pred":"db_id","subj":"T6","obj":"442234"},{"id":"A7","pred":"db_id","subj":"T7","obj":"2904946"},{"id":"A8","pred":"db_id","subj":"T8","obj":"2904946"},{"id":"A9","pred":"db_id","subj":"T9","obj":"209674"},{"id":"A10","pred":"db_id","subj":"T10","obj":"2904946"},{"id":"A11","pred":"db_id","subj":"T11","obj":"1369087"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T2","span":{"begin":134,"end":139},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":16,"end":21},"obj":"Glycan"},{"id":"T2","span":{"begin":193,"end":198},"obj":"Glycan"},{"id":"T3","span":{"begin":232,"end":238},"obj":"Glycan"},{"id":"T4","span":{"begin":318,"end":323},"obj":"Glycan"},{"id":"T5","span":{"begin":352,"end":358},"obj":"Glycan"},{"id":"T6","span":{"begin":438,"end":443},"obj":"Glycan"},{"id":"T7","span":{"begin":850,"end":855},"obj":"Glycan"},{"id":"T8","span":{"begin":946,"end":951},"obj":"Glycan"},{"id":"T9","span":{"begin":1207,"end":1214},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G79389NT"},{"id":"A7","pred":"glycosmos_id","subj":"T7","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A8","pred":"glycosmos_id","subj":"T8","obj":"https://glycosmos.org/glycans/show/G48558GR"},{"id":"A9","pred":"glycosmos_id","subj":"T9","obj":"https://glycosmos.org/glycans/show/G15541SE"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":232,"end":238},"obj":"Glycan"},{"id":"T2","span":{"begin":352,"end":358},"obj":"Glycan"},{"id":"T3","span":{"begin":1207,"end":1214},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A4","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A5","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G15541SE"},{"id":"A6","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T3","span":{"begin":134,"end":139},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":94},"obj":"Sentence"},{"id":"T2","span":{"begin":95,"end":172},"obj":"Sentence"},{"id":"T3","span":{"begin":173,"end":588},"obj":"Sentence"},{"id":"T4","span":{"begin":589,"end":806},"obj":"Sentence"},{"id":"T5","span":{"begin":807,"end":940},"obj":"Sentence"},{"id":"T6","span":{"begin":941,"end":1095},"obj":"Sentence"},{"id":"T7","span":{"begin":1096,"end":1215},"obj":"Sentence"},{"id":"T8","span":{"begin":1216,"end":1312},"obj":"Sentence"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T2","span":{"begin":134,"end":139},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:50801"},{"id":"A2","pred":"db_id","subj":"T2","obj":"FMA:50801"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T2","span":{"begin":134,"end":139},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":88,"end":93},"obj":"Body_part"},{"id":"T3","span":{"begin":134,"end":139},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"}],"text":"Purification of G-M-1-ganglioside and ceramide lactoside beta-galactosidase from rabbit brain.\nThe major beta-galactosidase of rabbit brain has been purified over 400-fold. The enzyme converts G-M-1-ganglioside; Gal beta-1 yields 3 GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-1) into Tay Sachs ganglioside GalNAc beta-1 yields 4 (NANalpha-2 yields 3) Gal beta-1 yields 4 Glc yields ceramide (G-M-2-ganglioside) and ceramide lactoside, Gal beta-1 yields 4 Glc yields ceramide (Gal-Glc-Cer) into glucocerebroside, Glc yields ceramide (Glc-Cer). The enzyme also hydrolyzes the synthetic substrates NPh-Gal and MeUmb-Gal. It is eluted as a single peak from Sephadex G-200 columns when natural and synthetic substrates were used and has an isoelectric point of 6.3. We were unable to resolve activity towards G-M-1-ganglioside and Gal-Glc-Cer by polyacrylamide electrophoresis in two buffer systems. With G-M-1 the pH optimum was 4.3 in acetate buffer and the K-m value 78 mu-M while with Gal-Glc-Cer, a pH optimum of 4.5 and a K-m of 17 mu-M were found. Hydrolysis of both natural and synthetic substrates was inhibited by gamma-D-galactonolactone, D-galactose and lactose. The data strongly suggest that a single beta-galactosidase hydrolyzes all the substrates tested."}