PubMed:23639970 JSON TXT

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OryzaGP_2021

OryzaGP_2022

{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":462,"end":463},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T2","span":{"begin":488,"end":489},"obj":"http://identifiers.org/oryzabase.gene/11216"}],"text":"Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.\nThe rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a 1 bp substitution in exon 1 (Jukkoku-type GA20ox-2 mutant allele: Jukkoku_GA20ox-2). An allele-specific primer (ASP)-polymerase chain reaction (PCR) with primers SD1F3 and SD1JR gave a PCR product specific to Jukkoku_GA20ox-2. In addition, ASP-PCR with primers SD1F3 and SD1NRM (which contains a mismatch at the third nucleotide from the 3'-terminus of SD1NR) gave a PCR product specific to non-Jukkoku_GA20ox-2. Multiplex ASP-PCR using SD1F3, VIC dye-labeled SD1JR, and FAM dye-labeled SD1NRM enabled simultaneous codominant detection of Jukkoku_GA20ox-2 and non-Jukkoku_GA20ox-2 among 188 cultivars. Also, Hikarishinseiki is identifiable by RM253 polymorphism from 11 cultivars carrying Jukkoku_GA20ox-2. Taken together, our results establish a methodology for distinguishing Hikarishinseiki."}

OryzaGP_2021_v2

{"project":"OryzaGP_2021_v2","denotations":[{"id":"T1","span":{"begin":84,"end":87},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T2","span":{"begin":246,"end":249},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T3","span":{"begin":392,"end":395},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T4","span":{"begin":504,"end":512},"obj":"http://identifiers.org/oryzabase.gene/470"},{"id":"T24188","span":{"begin":84,"end":87},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T48676","span":{"begin":246,"end":249},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T53819","span":{"begin":392,"end":395},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"},{"id":"T67039","span":{"begin":504,"end":512},"obj":"http://identifiers.org/rapdb.locus/Os01g0883800"}],"text":"Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.\nThe rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a 1 bp substitution in exon 1 (Jukkoku-type GA20ox-2 mutant allele: Jukkoku_GA20ox-2). An allele-specific primer (ASP)-polymerase chain reaction (PCR) with primers SD1F3 and SD1JR gave a PCR product specific to Jukkoku_GA20ox-2. In addition, ASP-PCR with primers SD1F3 and SD1NRM (which contains a mismatch at the third nucleotide from the 3'-terminus of SD1NR) gave a PCR product specific to non-Jukkoku_GA20ox-2. Multiplex ASP-PCR using SD1F3, VIC dye-labeled SD1JR, and FAM dye-labeled SD1NRM enabled simultaneous codominant detection of Jukkoku_GA20ox-2 and non-Jukkoku_GA20ox-2 among 188 cultivars. Also, Hikarishinseiki is identifiable by RM253 polymorphism from 11 cultivars carrying Jukkoku_GA20ox-2. Taken together, our results establish a methodology for distinguishing Hikarishinseiki."}

OryzaGP_2021_FLAIR

{"project":"OryzaGP_2021_FLAIR","denotations":[{"id":"M_0","span":{"begin":933,"end":936},"obj":"hunflair:NA:Chemical"},{"id":"M_1","span":{"begin":67,"end":92},"obj":"hunflair:NA:Gene"},{"id":"M_2","span":{"begin":229,"end":254},"obj":"hunflair:NA:Gene"},{"id":"M_3","span":{"begin":146,"end":175},"obj":"hunflair:NA:Species"},{"id":"M_4","span":{"begin":528,"end":544},"obj":"hunflair:NA:Gene"},{"id":"M_5","span":{"begin":671,"end":687},"obj":"hunflair:NA:Gene"},{"id":"M_6","span":{"begin":857,"end":873},"obj":"hunflair:NA:Gene"},{"id":"M_7","span":{"begin":1001,"end":1017},"obj":"hunflair:NA:Gene"},{"id":"M_8","span":{"begin":1026,"end":1042},"obj":"hunflair:NA:Gene"},{"id":"M_9","span":{"begin":1151,"end":1167},"obj":"hunflair:NA:Gene"},{"id":"M_10","span":{"begin":40,"end":44},"obj":"hunflair:NA:Species"},{"id":"M_11","span":{"begin":146,"end":150},"obj":"hunflair:NA:Species"},{"id":"M_12","span":{"begin":431,"end":453},"obj":"hunflair:NA:Gene"},{"id":"M_13","span":{"begin":84,"end":87},"obj":"hunflair:NA:Gene"},{"id":"M_14","span":{"begin":246,"end":249},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":392,"end":395},"obj":"hunflair:NA:Gene"}],"text":"Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.\nThe rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a 1 bp substitution in exon 1 (Jukkoku-type GA20ox-2 mutant allele: Jukkoku_GA20ox-2). An allele-specific primer (ASP)-polymerase chain reaction (PCR) with primers SD1F3 and SD1JR gave a PCR product specific to Jukkoku_GA20ox-2. In addition, ASP-PCR with primers SD1F3 and SD1NRM (which contains a mismatch at the third nucleotide from the 3'-terminus of SD1NR) gave a PCR product specific to non-Jukkoku_GA20ox-2. Multiplex ASP-PCR using SD1F3, VIC dye-labeled SD1JR, and FAM dye-labeled SD1NRM enabled simultaneous codominant detection of Jukkoku_GA20ox-2 and non-Jukkoku_GA20ox-2 among 188 cultivars. Also, Hikarishinseiki is identifiable by RM253 polymorphism from 11 cultivars carrying Jukkoku_GA20ox-2. Taken together, our results establish a methodology for distinguishing Hikarishinseiki."}

Allie

{"project":"Allie","denotations":[{"id":"SS1_23639970_3_0","span":{"begin":550,"end":572},"obj":"expanded"},{"id":"SS2_23639970_3_0","span":{"begin":574,"end":577},"obj":"abbr"}],"relations":[{"id":"AE1_23639970_3_0","pred":"abbreviatedTo","subj":"SS1_23639970_3_0","obj":"SS2_23639970_3_0"}],"text":"Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.\nThe rice cultivar Hikarishinseiki, a semidwarf isogenotype of Koshihikari carrying the Green Revolution sd1 gene, is increasingly grown in both Japan and the United States. Here, we report DNA diagnosis for Hikarishinseiki targeting its Jukkoku-type sd1 locus, which codes for a defective gibberellin 20-oxidase, with a 1 bp substitution in exon 1 (Jukkoku-type GA20ox-2 mutant allele: Jukkoku_GA20ox-2). An allele-specific primer (ASP)-polymerase chain reaction (PCR) with primers SD1F3 and SD1JR gave a PCR product specific to Jukkoku_GA20ox-2. In addition, ASP-PCR with primers SD1F3 and SD1NRM (which contains a mismatch at the third nucleotide from the 3'-terminus of SD1NR) gave a PCR product specific to non-Jukkoku_GA20ox-2. Multiplex ASP-PCR using SD1F3, VIC dye-labeled SD1JR, and FAM dye-labeled SD1NRM enabled simultaneous codominant detection of Jukkoku_GA20ox-2 and non-Jukkoku_GA20ox-2 among 188 cultivars. Also, Hikarishinseiki is identifiable by RM253 polymorphism from 11 cultivars carrying Jukkoku_GA20ox-2. Taken together, our results establish a methodology for distinguishing Hikarishinseiki."}