PubMed:234297 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/234297","sourcedb":"PubMed","sourceid":"234297","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/234297","text":"Purification, properties, and mode of action of hemicellulase I produced by Ceratocystis paradoxa.\nA culture isolate (CP2) of the fungal plant pathogen Ceratocystis paradoxa produces at least five extra-cellular hemicellulases when grown on a medium containing a commercial hemicellulose as inducer. One of the five enzymes, hemicellulase I (HC-I), was purified by ammonium sulphate preceipitation, ion-exchange chromatography (DEAE-Sephadex and then Cellex-CM), and iso-electric focusing at pH 3-10 and 8-10. HC-I behaves as a single protein on a electrophoresis at pH 6.0 and 8.4. The enzyme degrades hemicellulose B (an arabino-4-O-methylglucurono-xylan) and arabinoxylanto arabinose, xylose, xylobiose (Xyl2; beta-D-Xylp-(1 leads to 4)-D-Xyl), and a mixture of arabinose-xylose and xylose oligosaccharides (AraXyln and Xyln, where n=3, 4, or 5). The enzyme is deduced to be an endo-enzyme. Xylotetraose (Xyl4) was the lowest homologue of the xylose oligosaccharides attacked, yielding xylobiose and xylotriose (Xyl3) only. A mechanism is postulated for this reaction. AraXyl5 were slowly hydrolysed to arabinose and the respective xylose saccharide (Xyl2-Xyl5), and thence to Xyl2 and Xyl3. Hydrolysis of the arabinofuranosyl linkage probably does not occur at the same active site as for the xylose oligosaccharides. Hemicellulose B fractions from different sources appeared to be degraded by HC-I. The enzyme showed optimum activity at pH 5.5 and 40 degrees, and Km was 4.24 mg of hemicellulose/ml.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":428,"end":432},"obj":"HP_0000365"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":99,"end":299},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":300,"end":509},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":510,"end":582},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":583,"end":849},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":850,"end":893},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":894,"end":1026},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1027,"end":1071},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1072,"end":1194},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1195,"end":1321},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1322,"end":1403},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1404,"end":1504},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":98},"obj":"Sentence"},{"id":"T2","span":{"begin":99,"end":299},"obj":"Sentence"},{"id":"T3","span":{"begin":300,"end":509},"obj":"Sentence"},{"id":"T4","span":{"begin":510,"end":582},"obj":"Sentence"},{"id":"T5","span":{"begin":583,"end":849},"obj":"Sentence"},{"id":"T6","span":{"begin":850,"end":893},"obj":"Sentence"},{"id":"T7","span":{"begin":894,"end":1026},"obj":"Sentence"},{"id":"T8","span":{"begin":1027,"end":1071},"obj":"Sentence"},{"id":"T9","span":{"begin":1072,"end":1194},"obj":"Sentence"},{"id":"T10","span":{"begin":1195,"end":1321},"obj":"Sentence"},{"id":"T11","span":{"begin":1322,"end":1403},"obj":"Sentence"},{"id":"T12","span":{"begin":1404,"end":1504},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T12","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":76,"end":97},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":152,"end":173},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"13001"},{"id":"A2","pred":"db_id","subj":"T2","obj":"13001"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":342,"end":346},"obj":"Disease"},{"id":"T2","span":{"begin":510,"end":514},"obj":"Disease"},{"id":"T3","span":{"begin":1398,"end":1402},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0011191"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0011191"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0011191"},{"subj":"T1","pred":"source","obj":"mondo_disease"},{"subj":"T2","pred":"source","obj":"mondo_disease"},{"subj":"T3","pred":"source","obj":"mondo_disease"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":197,"end":211},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005576"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#e693ec","default":true},{"id":"sentences","color":"#93eccc"},{"id":"NCBITAXON","color":"#ecb293"},{"id":"mondo_disease","color":"#9893ec"},{"id":"Anatomy-UBERON","color":"#a7ec93"}]}]}}