| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-98 |
Sentence |
denotes |
Purification, properties, and mode of action of hemicellulase I produced by Ceratocystis paradoxa. |
| TextSentencer_T2 |
99-299 |
Sentence |
denotes |
A culture isolate (CP2) of the fungal plant pathogen Ceratocystis paradoxa produces at least five extra-cellular hemicellulases when grown on a medium containing a commercial hemicellulose as inducer. |
| TextSentencer_T3 |
300-509 |
Sentence |
denotes |
One of the five enzymes, hemicellulase I (HC-I), was purified by ammonium sulphate preceipitation, ion-exchange chromatography (DEAE-Sephadex and then Cellex-CM), and iso-electric focusing at pH 3-10 and 8-10. |
| TextSentencer_T4 |
510-582 |
Sentence |
denotes |
HC-I behaves as a single protein on a electrophoresis at pH 6.0 and 8.4. |
| TextSentencer_T5 |
583-849 |
Sentence |
denotes |
The enzyme degrades hemicellulose B (an arabino-4-O-methylglucurono-xylan) and arabinoxylanto arabinose, xylose, xylobiose (Xyl2; beta-D-Xylp-(1 leads to 4)-D-Xyl), and a mixture of arabinose-xylose and xylose oligosaccharides (AraXyln and Xyln, where n=3, 4, or 5). |
| TextSentencer_T6 |
850-893 |
Sentence |
denotes |
The enzyme is deduced to be an endo-enzyme. |
| TextSentencer_T7 |
894-1026 |
Sentence |
denotes |
Xylotetraose (Xyl4) was the lowest homologue of the xylose oligosaccharides attacked, yielding xylobiose and xylotriose (Xyl3) only. |
| TextSentencer_T8 |
1027-1071 |
Sentence |
denotes |
A mechanism is postulated for this reaction. |
| TextSentencer_T9 |
1072-1194 |
Sentence |
denotes |
AraXyl5 were slowly hydrolysed to arabinose and the respective xylose saccharide (Xyl2-Xyl5), and thence to Xyl2 and Xyl3. |
| TextSentencer_T10 |
1195-1321 |
Sentence |
denotes |
Hydrolysis of the arabinofuranosyl linkage probably does not occur at the same active site as for the xylose oligosaccharides. |
| TextSentencer_T11 |
1322-1403 |
Sentence |
denotes |
Hemicellulose B fractions from different sources appeared to be degraded by HC-I. |
| TextSentencer_T12 |
1404-1504 |
Sentence |
denotes |
The enzyme showed optimum activity at pH 5.5 and 40 degrees, and Km was 4.24 mg of hemicellulose/ml. |
| T1 |
0-98 |
Sentence |
denotes |
Purification, properties, and mode of action of hemicellulase I produced by Ceratocystis paradoxa. |
| T2 |
99-299 |
Sentence |
denotes |
A culture isolate (CP2) of the fungal plant pathogen Ceratocystis paradoxa produces at least five extra-cellular hemicellulases when grown on a medium containing a commercial hemicellulose as inducer. |
| T3 |
300-509 |
Sentence |
denotes |
One of the five enzymes, hemicellulase I (HC-I), was purified by ammonium sulphate preceipitation, ion-exchange chromatography (DEAE-Sephadex and then Cellex-CM), and iso-electric focusing at pH 3-10 and 8-10. |
| T4 |
510-582 |
Sentence |
denotes |
HC-I behaves as a single protein on a electrophoresis at pH 6.0 and 8.4. |
| T5 |
583-849 |
Sentence |
denotes |
The enzyme degrades hemicellulose B (an arabino-4-O-methylglucurono-xylan) and arabinoxylanto arabinose, xylose, xylobiose (Xyl2; beta-D-Xylp-(1 leads to 4)-D-Xyl), and a mixture of arabinose-xylose and xylose oligosaccharides (AraXyln and Xyln, where n=3, 4, or 5). |
| T6 |
850-893 |
Sentence |
denotes |
The enzyme is deduced to be an endo-enzyme. |
| T7 |
894-1026 |
Sentence |
denotes |
Xylotetraose (Xyl4) was the lowest homologue of the xylose oligosaccharides attacked, yielding xylobiose and xylotriose (Xyl3) only. |
| T8 |
1027-1071 |
Sentence |
denotes |
A mechanism is postulated for this reaction. |
| T9 |
1072-1194 |
Sentence |
denotes |
AraXyl5 were slowly hydrolysed to arabinose and the respective xylose saccharide (Xyl2-Xyl5), and thence to Xyl2 and Xyl3. |
| T10 |
1195-1321 |
Sentence |
denotes |
Hydrolysis of the arabinofuranosyl linkage probably does not occur at the same active site as for the xylose oligosaccharides. |
| T11 |
1322-1403 |
Sentence |
denotes |
Hemicellulose B fractions from different sources appeared to be degraded by HC-I. |
| T12 |
1404-1504 |
Sentence |
denotes |
The enzyme showed optimum activity at pH 5.5 and 40 degrees, and Km was 4.24 mg of hemicellulose/ml. |