PubMed:23307905 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/23307905","sourcedb":"PubMed","sourceid":"23307905","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/23307905","text":"A single amino acid toggles Escherichia coli polysialyltransferases between mono- and bifunctionality.\nThe enteropathogenic Escherichia coli K92 synthesizes a unique capsule consisting of polysialic acid (polySia) with alternating α2,8- and α2,9-linkages. The fact that a single enzyme is responsible for the synthesis of these alternating regioisomeric linkages raises questions as to how this controlled bifunctionality is achieved mechanistically. Aiming to identify the sequence elements responsible for dual regiospecificity, we have utilized a high-throughput polysialyltransferase (polyST) activity screen to explore the relevant sequence space between this enzyme and its close monofunctional homolog from E. coli K1. The linkage specificity of selected mutants was subsequently confirmed using a polySia permethylation linkage analysis technique. We have identified a single amino acid exchange at residue 52 that toggles these enzymes between mono and dual regiospecificity. The results have implications for the mechanism by which the E. coli K92 polyST achieves bifunctional elongation.","tracks":[{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":188,"end":203},"obj":"https://glytoucan.org/Structures/Glycans/G00065MO"},{"id":"T2","span":{"begin":205,"end":212},"obj":"https://glytoucan.org/Structures/Glycans/G00065MO"},{"id":"T3","span":{"begin":805,"end":812},"obj":"https://glytoucan.org/Structures/Glycans/G00065MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycan-Motif"},{"subj":"T2","pred":"source","obj":"Glycan-Motif"},{"subj":"T3","pred":"source","obj":"Glycan-Motif"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":188,"end":203},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":205,"end":212},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":805,"end":812},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00065MO"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00065MO"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00065MO"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":102},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":103,"end":255},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":256,"end":450},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":451,"end":725},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":726,"end":855},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":856,"end":984},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":985,"end":1098},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":102},"obj":"Sentence"},{"id":"T2","span":{"begin":103,"end":255},"obj":"Sentence"},{"id":"T3","span":{"begin":256,"end":450},"obj":"Sentence"},{"id":"T4","span":{"begin":451,"end":725},"obj":"Sentence"},{"id":"T5","span":{"begin":726,"end":855},"obj":"Sentence"},{"id":"T6","span":{"begin":856,"end":984},"obj":"Sentence"},{"id":"T7","span":{"begin":985,"end":1098},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":102},"obj":"Sentence"},{"id":"T2","span":{"begin":103,"end":255},"obj":"Sentence"},{"id":"T3","span":{"begin":256,"end":450},"obj":"Sentence"},{"id":"T4","span":{"begin":451,"end":725},"obj":"Sentence"},{"id":"T5","span":{"begin":726,"end":855},"obj":"Sentence"},{"id":"T6","span":{"begin":856,"end":984},"obj":"Sentence"},{"id":"T7","span":{"begin":985,"end":1098},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":188,"end":203},"obj":"https://glytoucan.org/Structures/Glycans/G00065MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlycoBiology-PACDB","denotations":[{"id":"_T1","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620"},{"id":"_T2","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636"},{"id":"_T3","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407"},{"id":"_T4","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452"},{"id":"_T5","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754"},{"id":"_T6","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354"},{"id":"_T7","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640"},{"id":"_T8","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487"},{"id":"_T9","span":{"begin":28,"end":44},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417"},{"id":"_T10","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417"},{"id":"_T11","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640"},{"id":"_T12","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636"},{"id":"_T13","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620"},{"id":"_T14","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754"},{"id":"_T15","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452"},{"id":"_T16","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407"},{"id":"_T17","span":{"begin":124,"end":140},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354"},{"id":"_T18","span":{"begin":124,"end":144},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC720"},{"id":"_T19","span":{"begin":124,"end":144},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T15","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T16","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T17","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T18","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T19","pred":"source","obj":"GlycoBiology-PACDB"}]},{"project":"ICD10","denotations":[{"id":"T1","span":{"begin":107,"end":140},"obj":"http://purl.bioontology.org/ontology/ICD10/A04.0"}],"attributes":[{"subj":"T1","pred":"source","obj":"ICD10"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":9,"end":19},"obj":"FMAID:82739"},{"id":"_T2","span":{"begin":9,"end":19},"obj":"FMAID:196728"},{"id":"_T3","span":{"begin":166,"end":173},"obj":"FMAID:85272"},{"id":"_T4","span":{"begin":166,"end":173},"obj":"FMAID:199625"},{"id":"_T5","span":{"begin":884,"end":894},"obj":"FMAID:82739"},{"id":"_T6","span":{"begin":884,"end":894},"obj":"FMAID:196728"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":722,"end":724},"obj":"http://www.uniprot.org/uniprot/P04264"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":722,"end":724},"obj":"http://www.uniprot.org/uniprot/P04104"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":28,"end":39},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/561"},{"id":"T2","span":{"begin":124,"end":135},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/561"},{"id":"T3","span":{"begin":1023,"end":1032},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/127244"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":309,"end":318},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T2","span":{"begin":895,"end":903},"obj":"http://purl.obolibrary.org/obo/GO_0015297"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"}]},{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":166,"end":173},"obj":"http://purl.obolibrary.org/obo/UBERON_0003893"}],"attributes":[{"subj":"T1","pred":"source","obj":"UBERON-AE"}]},{"project":"Allie","denotations":[{"id":"SS1_23307905_1_0","span":{"begin":188,"end":203},"obj":"expanded"},{"id":"SS2_23307905_1_0","span":{"begin":205,"end":212},"obj":"abbr"},{"id":"SS1_23307905_3_0","span":{"begin":566,"end":587},"obj":"expanded"},{"id":"SS2_23307905_3_0","span":{"begin":589,"end":595},"obj":"abbr"}],"relations":[{"id":"AE1_23307905_1_0","pred":"abbreviatedTo","subj":"SS1_23307905_1_0","obj":"SS2_23307905_1_0"},{"id":"AE1_23307905_3_0","pred":"abbreviatedTo","subj":"SS1_23307905_3_0","obj":"SS2_23307905_3_0"}],"attributes":[{"subj":"SS1_23307905_1_0","pred":"source","obj":"Allie"},{"subj":"SS2_23307905_1_0","pred":"source","obj":"Allie"},{"subj":"SS1_23307905_3_0","pred":"source","obj":"Allie"},{"subj":"SS2_23307905_3_0","pred":"source","obj":"Allie"}]},{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":915,"end":917},"obj":"http://www.glycoepitope.jp/epitopes/AN0785"}],"attributes":[{"subj":"PD-GlycoEpitope-B_T1","pred":"source","obj":"GlycoBiology-Epitope"}]},{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":166,"end":173},"obj":"http://purl.obolibrary.org/obo/UBERON_0003893"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":28,"end":44},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":124,"end":140},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":714,"end":721},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1046,"end":1053},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"562"},{"id":"A2","pred":"db_id","subj":"T2","obj":"562"},{"id":"A3","pred":"db_id","subj":"T3","obj":"562"},{"id":"A4","pred":"db_id","subj":"T4","obj":"562"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":646,"end":651},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000464"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Glycan-Motif","color":"#ec93c1","default":true},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#93dbec"},{"id":"sentences","color":"#ece393"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#c993ec"},{"id":"GlycoBiology-PACDB","color":"#93ecaf"},{"id":"ICD10","color":"#ec9593"},{"id":"GlycoBiology-FMA","color":"#93abec"},{"id":"uniprot-human","color":"#c5ec93"},{"id":"uniprot-mouse","color":"#ec93df"},{"id":"GlycoBiology-NCBITAXON","color":"#93ecdf"},{"id":"GO-BP","color":"#ecc593"},{"id":"UBERON-AE","color":"#ab93ec"},{"id":"Allie","color":"#94ec93"},{"id":"GlycoBiology-Epitope","color":"#ec93af"},{"id":"performance-test","color":"#93c9ec"},{"id":"NCBITAXON","color":"#e3ec93"},{"id":"Anatomy-UBERON","color":"#db93ec"}]}]}}