PubMed:23306612 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/23306612","sourcedb":"PubMed","sourceid":"23306612","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/23306612","text":"Inhibition of FOXM1 transcription factor suppresses cell proliferation and tumor growth of breast cancer.\nThe forkhead box M1 (FOXM1) transcription factor regulates the expression of genes essential for cell proliferation and transformation and is implicated in tumorigenesis and tumor progression. FOXM1 has been considered as a potential target for the prevention and/or therapeutic intervention in human carcinomas. In this study, we observed a strong expression of FOXM1 in clinical tissue specimens and cell lines of human breast cancer and a correlation between FOXM1 levels and the proliferation ability in the tested MCF-7, MDA-MB-231 and ZR-75-30 cells. By using an adenovirus vector (named AdFOXM1shRNA) that expresses a short hairpin RNA (shRNA) to downregulate FOXM1 expression specifically, we found that the knockdown of FOXM1 expression diminished the proliferation and anchorage-independent growth of the breast cancer cells. The FOXM1 silencing in ZR-75-30 cells dramatically prevented the tumorigenicity of the AdFOXM1shRNA-treated cells in vitro and in vivo. Furthermore, the efficacy of AdFOXM1shRNA for tumor gene therapy was assessed with the breast cancer xenograft mouse model and the tumor growth was significantly suppressed when inoculated mice were injected with AdFOXM1shRNA in the tumors. Together, our results suggest that FOXM1 is a potential therapeutic target for breast cancer and AdFOXM1shRNA may be an additional gene therapeutic intervention for breast cancer treatment.","tracks":[{"project":"Allie","denotations":[{"id":"SS1_23306612_1_0","span":{"begin":110,"end":125},"obj":"expanded"},{"id":"SS2_23306612_1_0","span":{"begin":127,"end":132},"obj":"abbr"},{"id":"SS1_23306612_4_0","span":{"begin":731,"end":748},"obj":"expanded"},{"id":"SS2_23306612_4_0","span":{"begin":750,"end":755},"obj":"abbr"}],"relations":[{"id":"AE1_23306612_1_0","pred":"abbreviatedTo","subj":"SS1_23306612_1_0","obj":"SS2_23306612_1_0"},{"id":"AE1_23306612_4_0","pred":"abbreviatedTo","subj":"SS1_23306612_4_0","obj":"SS2_23306612_4_0"}],"attributes":[{"subj":"SS1_23306612_1_0","pred":"source","obj":"Allie"},{"subj":"SS2_23306612_1_0","pred":"source","obj":"Allie"},{"subj":"SS1_23306612_4_0","pred":"source","obj":"Allie"},{"subj":"SS2_23306612_4_0","pred":"source","obj":"Allie"}]},{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":487,"end":493},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":91,"end":97},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":528,"end":534},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":921,"end":927},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":1165,"end":1171},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T6","span":{"begin":1398,"end":1404},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T7","span":{"begin":1484,"end":1490},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T3","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T4","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T5","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T6","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T7","pred":"source","obj":"performance-test"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":280,"end":285},"obj":"HP_0002664"},{"id":"T2","span":{"begin":528,"end":541},"obj":"HP_0003002"},{"id":"T3","span":{"begin":528,"end":541},"obj":"HP_0100013"},{"id":"T4","span":{"begin":535,"end":541},"obj":"HP_0002664"},{"id":"T5","span":{"begin":921,"end":934},"obj":"HP_0003002"},{"id":"T6","span":{"begin":921,"end":934},"obj":"HP_0100013"},{"id":"T7","span":{"begin":928,"end":934},"obj":"HP_0002664"},{"id":"T8","span":{"begin":1124,"end":1129},"obj":"HP_0002664"},{"id":"T9","span":{"begin":1165,"end":1178},"obj":"HP_0003002"},{"id":"T10","span":{"begin":1165,"end":1178},"obj":"HP_0100013"},{"id":"T11","span":{"begin":1172,"end":1178},"obj":"HP_0002664"},{"id":"T12","span":{"begin":1209,"end":1214},"obj":"HP_0002664"},{"id":"T13","span":{"begin":1311,"end":1317},"obj":"HP_0002664"},{"id":"T14","span":{"begin":1398,"end":1411},"obj":"HP_0003002"},{"id":"T15","span":{"begin":1398,"end":1411},"obj":"HP_0100013"},{"id":"T16","span":{"begin":1405,"end":1411},"obj":"HP_0002664"},{"id":"T17","span":{"begin":1484,"end":1497},"obj":"HP_0003002"},{"id":"T18","span":{"begin":1484,"end":1497},"obj":"HP_0100013"},{"id":"T19","span":{"begin":1491,"end":1497},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"},{"subj":"T4","pred":"source","obj":"PubmedHPO"},{"subj":"T5","pred":"source","obj":"PubmedHPO"},{"subj":"T6","pred":"source","obj":"PubmedHPO"},{"subj":"T7","pred":"source","obj":"PubmedHPO"},{"subj":"T8","pred":"source","obj":"PubmedHPO"},{"subj":"T9","pred":"source","obj":"PubmedHPO"},{"subj":"T10","pred":"source","obj":"PubmedHPO"},{"subj":"T11","pred":"source","obj":"PubmedHPO"},{"subj":"T12","pred":"source","obj":"PubmedHPO"},{"subj":"T13","pred":"source","obj":"PubmedHPO"},{"subj":"T14","pred":"source","obj":"PubmedHPO"},{"subj":"T15","pred":"source","obj":"PubmedHPO"},{"subj":"T16","pred":"source","obj":"PubmedHPO"},{"subj":"T17","pred":"source","obj":"PubmedHPO"},{"subj":"T18","pred":"source","obj":"PubmedHPO"},{"subj":"T19","pred":"source","obj":"PubmedHPO"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":14,"end":19},"obj":"gene:2305"},{"id":"T1","span":{"begin":91,"end":104},"obj":"disease:C0006142"},{"id":"T2","span":{"begin":14,"end":19},"obj":"gene:2305"},{"id":"T3","span":{"begin":91,"end":104},"obj":"disease:C0678222"},{"id":"T4","span":{"begin":110,"end":125},"obj":"gene:2305"},{"id":"T5","span":{"begin":262,"end":275},"obj":"disease:C0596263"},{"id":"T6","span":{"begin":110,"end":125},"obj":"gene:2305"},{"id":"T7","span":{"begin":280,"end":297},"obj":"disease:C0178874"},{"id":"T8","span":{"begin":127,"end":132},"obj":"gene:2305"},{"id":"T9","span":{"begin":262,"end":275},"obj":"disease:C0596263"},{"id":"T10","span":{"begin":127,"end":132},"obj":"gene:2305"},{"id":"T11","span":{"begin":280,"end":297},"obj":"disease:C0178874"},{"id":"T12","span":{"begin":299,"end":304},"obj":"gene:2305"},{"id":"T13","span":{"begin":407,"end":417},"obj":"disease:C0007097"},{"id":"T14","span":{"begin":568,"end":573},"obj":"gene:2305"},{"id":"T15","span":{"begin":528,"end":541},"obj":"disease:C0678222"},{"id":"T16","span":{"begin":469,"end":474},"obj":"gene:2305"},{"id":"T17","span":{"begin":528,"end":541},"obj":"disease:C0006142"},{"id":"T18","span":{"begin":568,"end":573},"obj":"gene:2305"},{"id":"T19","span":{"begin":528,"end":541},"obj":"disease:C0006142"},{"id":"T20","span":{"begin":469,"end":474},"obj":"gene:2305"},{"id":"T21","span":{"begin":528,"end":541},"obj":"disease:C0678222"},{"id":"T22","span":{"begin":835,"end":840},"obj":"gene:2305"},{"id":"T23","span":{"begin":921,"end":934},"obj":"disease:C0006142"},{"id":"T24","span":{"begin":835,"end":840},"obj":"gene:2305"},{"id":"T25","span":{"begin":921,"end":934},"obj":"disease:C0678222"},{"id":"T26","span":{"begin":1354,"end":1359},"obj":"gene:2305"},{"id":"T27","span":{"begin":1398,"end":1411},"obj":"disease:C0678222"},{"id":"T28","span":{"begin":1354,"end":1359},"obj":"gene:2305"},{"id":"T29","span":{"begin":1398,"end":1411},"obj":"disease:C0006142"},{"id":"T30","span":{"begin":1354,"end":1359},"obj":"gene:2305"},{"id":"T31","span":{"begin":1484,"end":1497},"obj":"disease:C0006142"},{"id":"T32","span":{"begin":1354,"end":1359},"obj":"gene:2305"},{"id":"T33","span":{"begin":1484,"end":1497},"obj":"disease:C0678222"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"},{"id":"R6","pred":"associated_with","subj":"T10","obj":"T11"},{"id":"R7","pred":"associated_with","subj":"T12","obj":"T13"},{"id":"R8","pred":"associated_with","subj":"T14","obj":"T15"},{"id":"R9","pred":"associated_with","subj":"T16","obj":"T17"},{"id":"R10","pred":"associated_with","subj":"T18","obj":"T19"},{"id":"R11","pred":"associated_with","subj":"T20","obj":"T21"},{"id":"R12","pred":"associated_with","subj":"T22","obj":"T23"},{"id":"R13","pred":"associated_with","subj":"T24","obj":"T25"},{"id":"R14","pred":"associated_with","subj":"T26","obj":"T27"},{"id":"R15","pred":"associated_with","subj":"T28","obj":"T29"},{"id":"R16","pred":"associated_with","subj":"T30","obj":"T31"},{"id":"R17","pred":"associated_with","subj":"T32","obj":"T33"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"},{"subj":"T6","pred":"source","obj":"DisGeNET"},{"subj":"T7","pred":"source","obj":"DisGeNET"},{"subj":"T8","pred":"source","obj":"DisGeNET"},{"subj":"T9","pred":"source","obj":"DisGeNET"},{"subj":"T10","pred":"source","obj":"DisGeNET"},{"subj":"T11","pred":"source","obj":"DisGeNET"},{"subj":"T12","pred":"source","obj":"DisGeNET"},{"subj":"T13","pred":"source","obj":"DisGeNET"},{"subj":"T14","pred":"source","obj":"DisGeNET"},{"subj":"T15","pred":"source","obj":"DisGeNET"},{"subj":"T16","pred":"source","obj":"DisGeNET"},{"subj":"T17","pred":"source","obj":"DisGeNET"},{"subj":"T18","pred":"source","obj":"DisGeNET"},{"subj":"T19","pred":"source","obj":"DisGeNET"},{"subj":"T20","pred":"source","obj":"DisGeNET"},{"subj":"T21","pred":"source","obj":"DisGeNET"},{"subj":"T22","pred":"source","obj":"DisGeNET"},{"subj":"T23","pred":"source","obj":"DisGeNET"},{"subj":"T24","pred":"source","obj":"DisGeNET"},{"subj":"T25","pred":"source","obj":"DisGeNET"},{"subj":"T26","pred":"source","obj":"DisGeNET"},{"subj":"T27","pred":"source","obj":"DisGeNET"},{"subj":"T28","pred":"source","obj":"DisGeNET"},{"subj":"T29","pred":"source","obj":"DisGeNET"},{"subj":"T30","pred":"source","obj":"DisGeNET"},{"subj":"T31","pred":"source","obj":"DisGeNET"},{"subj":"T32","pred":"source","obj":"DisGeNET"},{"subj":"T33","pred":"source","obj":"DisGeNET"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":106,"end":298},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":299,"end":418},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":419,"end":662},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":663,"end":941},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":942,"end":1077},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1078,"end":1318},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1319,"end":1508},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"T2","span":{"begin":106,"end":298},"obj":"Sentence"},{"id":"T3","span":{"begin":299,"end":418},"obj":"Sentence"},{"id":"T4","span":{"begin":419,"end":662},"obj":"Sentence"},{"id":"T5","span":{"begin":663,"end":941},"obj":"Sentence"},{"id":"T6","span":{"begin":942,"end":1077},"obj":"Sentence"},{"id":"T7","span":{"begin":1078,"end":1318},"obj":"Sentence"},{"id":"T8","span":{"begin":1319,"end":1508},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"UBERON-AE","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":91,"end":97},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":528,"end":534},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":921,"end":927},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":1165,"end":1171},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T5","span":{"begin":1398,"end":1404},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T6","span":{"begin":1484,"end":1490},"obj":"http://purl.obolibrary.org/obo/UBERON_0000310"},{"id":"PD-UBERON-AE-B_T7","span":{"begin":487,"end":493},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T3","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T4","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T5","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T6","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T7","pred":"source","obj":"UBERON-AE"}]},{"project":"DisGeNet-2017-sample","denotations":[{"id":"T2794","span":{"begin":14,"end":19},"obj":"gene:2305"},{"id":"T2795","span":{"begin":91,"end":104},"obj":"disease:C0006142"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T2794","obj":"T2795"},{"id":"R2","pred":"associated_with","subj":"T2794","obj":"T2795"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T2794","pred":"source","obj":"DisGeNet-2017-sample"},{"subj":"T2795","pred":"source","obj":"DisGeNet-2017-sample"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"23306612-0#14#19#gene2305","span":{"begin":14,"end":19},"obj":"gene2305"},{"id":"23306612-0#91#104#diseaseC0006142","span":{"begin":91,"end":104},"obj":"diseaseC0006142"},{"id":"23306612-0#91#104#diseaseC0678222","span":{"begin":91,"end":104},"obj":"diseaseC0678222"},{"id":"23306612-1#4#19#gene2305","span":{"begin":110,"end":125},"obj":"gene2305"},{"id":"23306612-1#21#26#gene2305","span":{"begin":127,"end":132},"obj":"gene2305"},{"id":"23306612-1#156#169#diseaseC0596263","span":{"begin":262,"end":275},"obj":"diseaseC0596263"},{"id":"23306612-1#174#191#diseaseC0178874","span":{"begin":280,"end":297},"obj":"diseaseC0178874"},{"id":"23306612-1#156#169#diseaseC0596263","span":{"begin":262,"end":275},"obj":"diseaseC0596263"},{"id":"23306612-1#174#191#diseaseC0178874","span":{"begin":280,"end":297},"obj":"diseaseC0178874"}],"relations":[{"id":"14#19#gene230591#104#diseaseC0006142","pred":"associated_with","subj":"23306612-0#14#19#gene2305","obj":"23306612-0#91#104#diseaseC0006142"},{"id":"14#19#gene230591#104#diseaseC0678222","pred":"associated_with","subj":"23306612-0#14#19#gene2305","obj":"23306612-0#91#104#diseaseC0678222"},{"id":"4#19#gene2305156#169#diseaseC0596263","pred":"associated_with","subj":"23306612-1#4#19#gene2305","obj":"23306612-1#156#169#diseaseC0596263"},{"id":"4#19#gene2305174#191#diseaseC0178874","pred":"associated_with","subj":"23306612-1#4#19#gene2305","obj":"23306612-1#174#191#diseaseC0178874"},{"id":"4#19#gene2305156#169#diseaseC0596263","pred":"associated_with","subj":"23306612-1#4#19#gene2305","obj":"23306612-1#156#169#diseaseC0596263"},{"id":"4#19#gene2305174#191#diseaseC0178874","pred":"associated_with","subj":"23306612-1#4#19#gene2305","obj":"23306612-1#174#191#diseaseC0178874"},{"id":"21#26#gene2305156#169#diseaseC0596263","pred":"associated_with","subj":"23306612-1#21#26#gene2305","obj":"23306612-1#156#169#diseaseC0596263"},{"id":"21#26#gene2305174#191#diseaseC0178874","pred":"associated_with","subj":"23306612-1#21#26#gene2305","obj":"23306612-1#174#191#diseaseC0178874"},{"id":"21#26#gene2305156#169#diseaseC0596263","pred":"associated_with","subj":"23306612-1#21#26#gene2305","obj":"23306612-1#156#169#diseaseC0596263"},{"id":"21#26#gene2305174#191#diseaseC0178874","pred":"associated_with","subj":"23306612-1#21#26#gene2305","obj":"23306612-1#174#191#diseaseC0178874"}],"attributes":[{"subj":"23306612-0#14#19#gene2305","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-0#91#104#diseaseC0006142","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-0#91#104#diseaseC0678222","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#4#19#gene2305","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#21#26#gene2305","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#156#169#diseaseC0596263","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#174#191#diseaseC0178874","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#156#169#diseaseC0596263","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"23306612-1#174#191#diseaseC0178874","pred":"source","obj":"DisGeNET5_gene_disease"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Allie","color":"#ec93d5","default":true},{"id":"performance-test","color":"#93ece9"},{"id":"PubmedHPO","color":"#eccf93"},{"id":"DisGeNET","color":"#b593ec"},{"id":"sentences","color":"#93ec9b"},{"id":"UBERON-AE","color":"#ec93a5"},{"id":"DisGeNet-2017-sample","color":"#93bfec"},{"id":"DisGeNET5_gene_disease","color":"#d9ec93"}]}]}}