PubMed:2263449 JSON TXT

Annnotations TAB JSON ListView MergeView

OryzaGP_2021

OryzaGP_2022

{"project":"OryzaGP_2022","denotations":[{"id":"T1","span":{"begin":40,"end":48},"obj":"http://identifiers.org/oryzabase.gene/22725"},{"id":"T2","span":{"begin":40,"end":48},"obj":"http://identifiers.org/oryzabase.gene/13733"},{"id":"T3","span":{"begin":185,"end":193},"obj":"http://identifiers.org/oryzabase.gene/22725"},{"id":"T4","span":{"begin":185,"end":193},"obj":"http://identifiers.org/oryzabase.gene/13733"},{"id":"T5","span":{"begin":205,"end":206},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T6","span":{"begin":640,"end":641},"obj":"http://identifiers.org/oryzabase.gene/11216"},{"id":"T7","span":{"begin":796,"end":797},"obj":"http://identifiers.org/oryzabase.gene/11216"}],"text":"Multiple protein factors bind to a rice glutelin promoter region.\nOur goal is to identify cis-acting elements in the regulatory region of the major seed storage protein gene in rice. A glutelin gene (pGL5-1) has been cloned by screening a rice genomic DNA library with synthetic oligonucleotides and with an amplified DNA fragment. A transient expression assay using immature rice seeds shows that its 5' flanking sequence can direct the synthesis of beta-glucuronidase (GUS) when fused upstream of the GUS coding region. Gel-retardation assays were performed to study protein-DNA interactions between putative regulatory sequences of pGL5-1 and nuclear proteins from immature rice seeds. We demonstrate that at least six protein-DNA complexes are formed between the 5' flanking sequence of pGL5-1 (-677 to -45) and nuclear protein factors. By subsequent DNase I-footprinting analyses we defined several protein-binding regions. Two of the protein-binding sequences contain the TGAGTCA motif, which is also present in the -300 element found in the 5' flanking sequences of several storage protein genes of other crop plants, and to which the transcription factors jun and GCN4 bind."}

OryzaGP_2021_v2

{"project":"OryzaGP_2021_v2","denotations":[{"id":"T1","span":{"begin":200,"end":204},"obj":"http://identifiers.org/oryzabase.gene/22282"},{"id":"T2","span":{"begin":635,"end":639},"obj":"http://identifiers.org/oryzabase.gene/22282"},{"id":"T3","span":{"begin":791,"end":795},"obj":"http://identifiers.org/oryzabase.gene/22282"},{"id":"T90747","span":{"begin":200,"end":204},"obj":"http://identifiers.org/rapdb.locus/Os06g0611400"},{"id":"T41113","span":{"begin":635,"end":639},"obj":"http://identifiers.org/rapdb.locus/Os06g0611400"},{"id":"T81934","span":{"begin":791,"end":795},"obj":"http://identifiers.org/rapdb.locus/Os06g0611400"}],"text":"Multiple protein factors bind to a rice glutelin promoter region.\nOur goal is to identify cis-acting elements in the regulatory region of the major seed storage protein gene in rice. A glutelin gene (pGL5-1) has been cloned by screening a rice genomic DNA library with synthetic oligonucleotides and with an amplified DNA fragment. A transient expression assay using immature rice seeds shows that its 5' flanking sequence can direct the synthesis of beta-glucuronidase (GUS) when fused upstream of the GUS coding region. Gel-retardation assays were performed to study protein-DNA interactions between putative regulatory sequences of pGL5-1 and nuclear proteins from immature rice seeds. We demonstrate that at least six protein-DNA complexes are formed between the 5' flanking sequence of pGL5-1 (-677 to -45) and nuclear protein factors. By subsequent DNase I-footprinting analyses we defined several protein-binding regions. Two of the protein-binding sequences contain the TGAGTCA motif, which is also present in the -300 element found in the 5' flanking sequences of several storage protein genes of other crop plants, and to which the transcription factors jun and GCN4 bind."}

OryzaGP_2021_FLAIR

{"project":"OryzaGP_2021_FLAIR","denotations":[{"id":"M_0","span":{"begin":1142,"end":1163},"obj":"hunflair:NA:Gene"},{"id":"M_1","span":{"begin":451,"end":469},"obj":"hunflair:NA:Gene"},{"id":"M_2","span":{"begin":1117,"end":1123},"obj":"hunflair:NA:Species"},{"id":"M_3","span":{"begin":35,"end":39},"obj":"hunflair:NA:Species"},{"id":"M_4","span":{"begin":177,"end":181},"obj":"hunflair:NA:Species"},{"id":"M_5","span":{"begin":239,"end":243},"obj":"hunflair:NA:Species"},{"id":"M_6","span":{"begin":376,"end":380},"obj":"hunflair:NA:Species"},{"id":"M_7","span":{"begin":677,"end":681},"obj":"hunflair:NA:Species"},{"id":"M_8","span":{"begin":816,"end":839},"obj":"hunflair:NA:Gene"},{"id":"M_9","span":{"begin":471,"end":474},"obj":"hunflair:NA:Gene"},{"id":"M_10","span":{"begin":503,"end":506},"obj":"hunflair:NA:Gene"},{"id":"M_11","span":{"begin":200,"end":206},"obj":"hunflair:NA:Gene"},{"id":"M_12","span":{"begin":635,"end":641},"obj":"hunflair:NA:Gene"},{"id":"M_13","span":{"begin":791,"end":797},"obj":"hunflair:NA:Gene"},{"id":"M_14","span":{"begin":1172,"end":1176},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":855,"end":862},"obj":"hunflair:NA:Gene"},{"id":"M_16","span":{"begin":40,"end":48},"obj":"hunflair:NA:Gene"},{"id":"M_17","span":{"begin":185,"end":193},"obj":"hunflair:NA:Gene"}],"text":"Multiple protein factors bind to a rice glutelin promoter region.\nOur goal is to identify cis-acting elements in the regulatory region of the major seed storage protein gene in rice. A glutelin gene (pGL5-1) has been cloned by screening a rice genomic DNA library with synthetic oligonucleotides and with an amplified DNA fragment. A transient expression assay using immature rice seeds shows that its 5' flanking sequence can direct the synthesis of beta-glucuronidase (GUS) when fused upstream of the GUS coding region. Gel-retardation assays were performed to study protein-DNA interactions between putative regulatory sequences of pGL5-1 and nuclear proteins from immature rice seeds. We demonstrate that at least six protein-DNA complexes are formed between the 5' flanking sequence of pGL5-1 (-677 to -45) and nuclear protein factors. By subsequent DNase I-footprinting analyses we defined several protein-binding regions. Two of the protein-binding sequences contain the TGAGTCA motif, which is also present in the -300 element found in the 5' flanking sequences of several storage protein genes of other crop plants, and to which the transcription factors jun and GCN4 bind."}

Oryza_sentences

{"project":"Oryza_sentences","blocks":[{"id":"T1","span":{"begin":0,"end":65},"obj":"Sentence"},{"id":"T2","span":{"begin":66,"end":182},"obj":"Sentence"},{"id":"T3","span":{"begin":183,"end":331},"obj":"Sentence"},{"id":"T4","span":{"begin":332,"end":521},"obj":"Sentence"},{"id":"T5","span":{"begin":522,"end":688},"obj":"Sentence"},{"id":"T6","span":{"begin":689,"end":840},"obj":"Sentence"},{"id":"T7","span":{"begin":841,"end":928},"obj":"Sentence"},{"id":"T8","span":{"begin":929,"end":1182},"obj":"Sentence"}],"text":"Multiple protein factors bind to a rice glutelin promoter region.\nOur goal is to identify cis-acting elements in the regulatory region of the major seed storage protein gene in rice. A glutelin gene (pGL5-1) has been cloned by screening a rice genomic DNA library with synthetic oligonucleotides and with an amplified DNA fragment. A transient expression assay using immature rice seeds shows that its 5' flanking sequence can direct the synthesis of beta-glucuronidase (GUS) when fused upstream of the GUS coding region. Gel-retardation assays were performed to study protein-DNA interactions between putative regulatory sequences of pGL5-1 and nuclear proteins from immature rice seeds. We demonstrate that at least six protein-DNA complexes are formed between the 5' flanking sequence of pGL5-1 (-677 to -45) and nuclear protein factors. By subsequent DNase I-footprinting analyses we defined several protein-binding regions. Two of the protein-binding sequences contain the TGAGTCA motif, which is also present in the -300 element found in the 5' flanking sequences of several storage protein genes of other crop plants, and to which the transcription factors jun and GCN4 bind."}