> top > docs > PubMed:22418094 > annotations

PubMed:22418094 JSONTXT

Annnotations TAB JSON ListView MergeView

OryzaGP_2021

Id Subject Object Predicate Lexical cue
T1 5-10 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T2 122-127 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T3 611-616 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T4 809-814 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T5 856-861 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T6 1010-1015 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T7 1022-1027 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T8 1966-1971 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T51362 5-10 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T64297 122-127 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T54381 611-616 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T13491 809-814 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T42588 856-861 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T59195 1010-1015 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T85201 1022-1027 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T69044 1966-1971 http://identifiers.org/ricegap/LOC_Os03g49600 denotes BGlu1
T22731 5-10 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T60932 122-127 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T63517 611-616 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T89176 809-814 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T51058 856-861 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T34076 1010-1015 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T84135 1022-1027 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T70694 1966-1971 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T48399 5-10 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T61073 122-127 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T36009 611-616 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T72053 809-814 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T81949 856-861 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T69830 1010-1015 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T35305 1022-1027 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
T90337 1966-1971 http://identifiers.org/uniprot/Q75I93 denotes BGlu1
M_0 247-274 hunflair:NA:Chemical denotes α-d-glucopyranosyl fluoride
M_1 1471-1490 hunflair:NA:Chemical denotes α-mannosyl fluoride
M_2 86-100 hunflair:NA:Chemical denotes cyclophellitol
M_3 912-926 hunflair:NA:Chemical denotes cyclophellitol
M_4 345-360 hunflair:NA:Chemical denotes oligosaccharide
M_5 531-538 hunflair:NA:Chemical denotes Fuc-pNP
M_6 671-678 hunflair:NA:Chemical denotes Fuc-pNP
M_7 700-707 hunflair:NA:Chemical denotes Fuc-pNP
M_8 721-728 hunflair:NA:Chemical denotes Fuc-pNP
M_9 758-765 hunflair:NA:Chemical denotes Fuc-pNP
M_10 1678-1685 hunflair:NA:Chemical denotes Fuc-pNP
M_11 1326-1347 hunflair:NA:Chemical denotes α-D-mannosyl fluoride
M_12 688-707 hunflair:NA:Chemical denotes Fuc-β-(1→3)-Fuc-pNP
M_13 1353-1373 hunflair:NA:Chemical denotes α-D-xylosyl fluoride
M_14 220-241 hunflair:NA:Chemical denotes cellooligosaccharides
M_15 1657-1667 hunflair:NA:Chemical denotes cellobiose
M_16 1254-1277 hunflair:NA:Chemical denotes α-L-arabinosyl fluoride
M_17 507-529 hunflair:NA:Chemical denotes pNP-β-D-fucopyranoside
M_18 1542-1573 hunflair:NA:Chemical denotes β-(1→4)-linked glycosyl residue
M_19 1687-1712 hunflair:NA:Chemical denotes pNP-β-D-galactopyranoside
M_20 292-305 hunflair:NA:Chemical denotes p-nitrophenyl
M_21 1301-1324 hunflair:NA:Chemical denotes α-D-galactosyl fluoride
M_22 1718-1740 hunflair:NA:Chemical denotes pNP-β-D-xylopyranoside
M_23 0-4 hunflair:NA:Species denotes Rice
M_24 1017-1021 hunflair:NA:Species denotes Rice
M_25 611-630 hunflair:NA:Gene denotes BGlu1 β-glucosidase
M_26 856-875 hunflair:NA:Gene denotes BGlu1 β-glucosidase
M_27 1017-1035 hunflair:NA:Gene denotes Rice BGlu1 E386G-2
M_28 312-324 hunflair:NA:Chemical denotes cellobioside
M_29 117-121 hunflair:NA:Species denotes rice
M_30 5-10 hunflair:NA:Gene denotes BGlu1
M_31 122-127 hunflair:NA:Gene denotes BGlu1
M_32 611-616 hunflair:NA:Gene denotes BGlu1
M_33 809-814 hunflair:NA:Gene denotes BGlu1
M_34 856-861 hunflair:NA:Gene denotes BGlu1
M_35 1010-1015 hunflair:NA:Gene denotes BGlu1
M_36 1022-1027 hunflair:NA:Gene denotes BGlu1
M_37 1966-1971 hunflair:NA:Gene denotes BGlu1
M_38 494-501 hunflair:NA:Chemical denotes Glc-pNP
M_39 1669-1676 hunflair:NA:Chemical denotes Glc-pNP
M_40 1976-1991 hunflair:NA:Gene denotes G glycosynthase
M_41 1028-1035 hunflair:NA:Gene denotes E386G-2
M_42 1195-1202 hunflair:NA:Gene denotes E386G-2
M_43 307-310 hunflair:NA:Chemical denotes pNP
M_44 331-334 hunflair:NA:Chemical denotes pNP
M_45 469-472 hunflair:NA:Chemical denotes pNP
M_46 498-501 hunflair:NA:Chemical denotes pNP
M_47 507-510 hunflair:NA:Chemical denotes pNP
M_48 535-538 hunflair:NA:Chemical denotes pNP
M_49 543-546 hunflair:NA:Chemical denotes pNP
M_50 675-678 hunflair:NA:Chemical denotes pNP
M_51 704-707 hunflair:NA:Chemical denotes pNP
M_52 725-728 hunflair:NA:Chemical denotes pNP
M_53 762-765 hunflair:NA:Chemical denotes pNP
M_54 1389-1392 hunflair:NA:Chemical denotes pNP
M_55 1673-1676 hunflair:NA:Chemical denotes pNP
M_56 1682-1685 hunflair:NA:Chemical denotes pNP
M_57 1687-1690 hunflair:NA:Chemical denotes pNP
M_58 1718-1721 hunflair:NA:Chemical denotes pNP
M_59 1766-1769 hunflair:NA:Chemical denotes pNP
M_60 469-492 hunflair:NA:Chemical denotes pNP-β-D-glucopyranoside
M_61 442-448 hunflair:NA:Gene denotes enzyme
M_62 1812-1828 hunflair:NA:Chemical denotes oligosaccharides
M_63 734-765 hunflair:NA:Chemical denotes Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP
M_64 563-569 hunflair:NA:Chemical denotes fucose
M_65 326-334 hunflair:NA:Chemical denotes Glc2-pNP
M_66 1384-1392 hunflair:NA:Chemical denotes Glc2-pNP
M_67 709-728 hunflair:NA:Chemical denotes Glc-β-(1→3)-Fuc-pNP
M_68 746-765 hunflair:NA:Chemical denotes Glc-β-(1→3)-Fuc-pNP
M_69 122-143 hunflair:NA:Gene denotes BGlu1 β-D-glucosidase
M_70 1448-1466 hunflair:NA:Chemical denotes α-fucosyl fluoride
M_71 11-24 hunflair:NA:Gene denotes glycosynthase
M_72 174-187 hunflair:NA:Gene denotes glycosynthase
M_73 823-836 hunflair:NA:Gene denotes glycosynthase
M_74 952-965 hunflair:NA:Gene denotes glycosynthase
M_75 1111-1124 hunflair:NA:Gene denotes glycosynthase
M_76 1595-1608 hunflair:NA:Gene denotes glycosynthase
M_77 1978-1991 hunflair:NA:Gene denotes glycosynthase
M_78 276-280 hunflair:NA:Chemical denotes GlcF
M_79 662-666 hunflair:NA:Chemical denotes GlcF
M_80 1248-1252 hunflair:NA:Chemical denotes GlcF
M_81 1634-1638 hunflair:NA:Chemical denotes GlcF
M_82 1225-1242 hunflair:NA:Chemical denotes glycosyl residues
M_83 551-558 hunflair:NA:Chemical denotes glucose
M_84 1621-1628 hunflair:NA:Chemical denotes glucose
M_85 1648-1655 hunflair:NA:Chemical denotes glucose
M_86 1279-1299 hunflair:NA:Chemical denotes α-D-fucosyl fluoride
M_87 1766-1789 hunflair:NA:Chemical denotes pNP-β-D-mannopyranoside
M_88 0-24 hunflair:NA:Gene denotes Rice BGlu1 glycosynthase

OryzaGP_2021_v2

Id Subject Object Predicate Lexical cue
T1 5-10 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T2 122-127 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T3 611-616 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T4 809-814 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T5 856-861 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T6 1010-1015 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T7 1022-1027 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T8 1966-1971 http://identifiers.org/oryzabase.gene/12081 denotes BGlu1
T318 5-10 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T46596 122-127 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T43560 611-616 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T91385 809-814 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T35465 856-861 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T59900 1010-1015 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T33665 1022-1027 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1
T14517 1966-1971 http://identifiers.org/rapdb.locus/Os03g0703000 denotes BGlu1

OryzaGP_2022

Id Subject Object Predicate Lexical cue
T1 695-696 http://identifiers.org/oryzabase.gene/11216 denotes 1
T2 716-717 http://identifiers.org/oryzabase.gene/11216 denotes 1
T3 741-742 http://identifiers.org/oryzabase.gene/11216 denotes 1
T4 753-754 http://identifiers.org/oryzabase.gene/11216 denotes 1
T5 1545-1546 http://identifiers.org/oryzabase.gene/11216 denotes 1

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 551-558 Glycan_Motif denotes glucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG
T2 563-569 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T3 1621-1628 Glycan_Motif denotes glucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG
T4 1648-1655 Glycan_Motif denotes glucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 551-558 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose
T2 563-569 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T3 1621-1628 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose
T4 1648-1655 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose

OryzaGP_2021_FLAIR

Id Subject Object Predicate Lexical cue
M_0 247-274 hunflair:NA:Chemical denotes α-d-glucopyranosyl fluoride
M_1 1471-1490 hunflair:NA:Chemical denotes α-mannosyl fluoride
M_2 86-100 hunflair:NA:Chemical denotes cyclophellitol
M_3 912-926 hunflair:NA:Chemical denotes cyclophellitol
M_4 345-360 hunflair:NA:Chemical denotes oligosaccharide
M_5 531-538 hunflair:NA:Chemical denotes Fuc-pNP
M_6 671-678 hunflair:NA:Chemical denotes Fuc-pNP
M_7 700-707 hunflair:NA:Chemical denotes Fuc-pNP
M_8 721-728 hunflair:NA:Chemical denotes Fuc-pNP
M_9 758-765 hunflair:NA:Chemical denotes Fuc-pNP
M_10 1678-1685 hunflair:NA:Chemical denotes Fuc-pNP
M_11 1326-1347 hunflair:NA:Chemical denotes α-D-mannosyl fluoride
M_12 688-707 hunflair:NA:Chemical denotes Fuc-β-(1→3)-Fuc-pNP
M_13 1353-1373 hunflair:NA:Chemical denotes α-D-xylosyl fluoride
M_14 220-241 hunflair:NA:Chemical denotes cellooligosaccharides
M_15 1657-1667 hunflair:NA:Chemical denotes cellobiose
M_16 1254-1277 hunflair:NA:Chemical denotes α-L-arabinosyl fluoride
M_17 507-529 hunflair:NA:Chemical denotes pNP-β-D-fucopyranoside
M_18 1542-1573 hunflair:NA:Chemical denotes β-(1→4)-linked glycosyl residue
M_19 1687-1712 hunflair:NA:Chemical denotes pNP-β-D-galactopyranoside
M_20 292-305 hunflair:NA:Chemical denotes p-nitrophenyl
M_21 1301-1324 hunflair:NA:Chemical denotes α-D-galactosyl fluoride
M_22 1718-1740 hunflair:NA:Chemical denotes pNP-β-D-xylopyranoside
M_23 0-4 hunflair:NA:Species denotes Rice
M_24 1017-1021 hunflair:NA:Species denotes Rice
M_25 611-630 hunflair:NA:Gene denotes BGlu1 β-glucosidase
M_26 856-875 hunflair:NA:Gene denotes BGlu1 β-glucosidase
M_27 1017-1035 hunflair:NA:Gene denotes Rice BGlu1 E386G-2
M_28 312-324 hunflair:NA:Chemical denotes cellobioside
M_29 117-121 hunflair:NA:Species denotes rice
M_30 5-10 hunflair:NA:Gene denotes BGlu1
M_31 122-127 hunflair:NA:Gene denotes BGlu1
M_32 611-616 hunflair:NA:Gene denotes BGlu1
M_33 809-814 hunflair:NA:Gene denotes BGlu1
M_34 856-861 hunflair:NA:Gene denotes BGlu1
M_35 1010-1015 hunflair:NA:Gene denotes BGlu1
M_36 1022-1027 hunflair:NA:Gene denotes BGlu1
M_37 1966-1971 hunflair:NA:Gene denotes BGlu1
M_38 494-501 hunflair:NA:Chemical denotes Glc-pNP
M_39 1669-1676 hunflair:NA:Chemical denotes Glc-pNP
M_40 1976-1991 hunflair:NA:Gene denotes G glycosynthase
M_41 1028-1035 hunflair:NA:Gene denotes E386G-2
M_42 1195-1202 hunflair:NA:Gene denotes E386G-2
M_43 307-310 hunflair:NA:Chemical denotes pNP
M_44 331-334 hunflair:NA:Chemical denotes pNP
M_45 469-472 hunflair:NA:Chemical denotes pNP
M_46 498-501 hunflair:NA:Chemical denotes pNP
M_47 507-510 hunflair:NA:Chemical denotes pNP
M_48 535-538 hunflair:NA:Chemical denotes pNP
M_49 543-546 hunflair:NA:Chemical denotes pNP
M_50 675-678 hunflair:NA:Chemical denotes pNP
M_51 704-707 hunflair:NA:Chemical denotes pNP
M_52 725-728 hunflair:NA:Chemical denotes pNP
M_53 762-765 hunflair:NA:Chemical denotes pNP
M_54 1389-1392 hunflair:NA:Chemical denotes pNP
M_55 1673-1676 hunflair:NA:Chemical denotes pNP
M_56 1682-1685 hunflair:NA:Chemical denotes pNP
M_57 1687-1690 hunflair:NA:Chemical denotes pNP
M_58 1718-1721 hunflair:NA:Chemical denotes pNP
M_59 1766-1769 hunflair:NA:Chemical denotes pNP
M_60 469-492 hunflair:NA:Chemical denotes pNP-β-D-glucopyranoside
M_61 442-448 hunflair:NA:Gene denotes enzyme
M_62 1812-1828 hunflair:NA:Chemical denotes oligosaccharides
M_63 734-765 hunflair:NA:Chemical denotes Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP
M_64 563-569 hunflair:NA:Chemical denotes fucose
M_65 326-334 hunflair:NA:Chemical denotes Glc2-pNP
M_66 1384-1392 hunflair:NA:Chemical denotes Glc2-pNP
M_67 709-728 hunflair:NA:Chemical denotes Glc-β-(1→3)-Fuc-pNP
M_68 746-765 hunflair:NA:Chemical denotes Glc-β-(1→3)-Fuc-pNP
M_69 122-143 hunflair:NA:Gene denotes BGlu1 β-D-glucosidase
M_70 1448-1466 hunflair:NA:Chemical denotes α-fucosyl fluoride
M_71 11-24 hunflair:NA:Gene denotes glycosynthase
M_72 174-187 hunflair:NA:Gene denotes glycosynthase
M_73 823-836 hunflair:NA:Gene denotes glycosynthase
M_74 952-965 hunflair:NA:Gene denotes glycosynthase
M_75 1111-1124 hunflair:NA:Gene denotes glycosynthase
M_76 1595-1608 hunflair:NA:Gene denotes glycosynthase
M_77 1978-1991 hunflair:NA:Gene denotes glycosynthase
M_78 276-280 hunflair:NA:Chemical denotes GlcF
M_79 662-666 hunflair:NA:Chemical denotes GlcF
M_80 1248-1252 hunflair:NA:Chemical denotes GlcF
M_81 1634-1638 hunflair:NA:Chemical denotes GlcF
M_82 1225-1242 hunflair:NA:Chemical denotes glycosyl residues
M_83 551-558 hunflair:NA:Chemical denotes glucose
M_84 1621-1628 hunflair:NA:Chemical denotes glucose
M_85 1648-1655 hunflair:NA:Chemical denotes glucose
M_86 1279-1299 hunflair:NA:Chemical denotes α-D-fucosyl fluoride
M_87 1766-1789 hunflair:NA:Chemical denotes pNP-β-D-mannopyranoside
M_88 0-24 hunflair:NA:Gene denotes Rice BGlu1 glycosynthase

Allie

Id Subject Object Predicate Lexical cue
SS1_22418094_1_0 247-274 expanded denotes α-d-glucopyranosyl fluoride
SS2_22418094_1_0 276-280 abbr denotes GlcF
SS1_22418094_1_1 292-305 expanded denotes p-nitrophenyl
SS2_22418094_1_1 307-310 abbr denotes pNP
SS1_22418094_2_0 469-492 expanded denotes pNP-β-D-glucopyranoside
SS2_22418094_2_0 494-501 abbr denotes Glc-pNP
SS1_22418094_2_1 507-529 expanded denotes pNP-β-D-fucopyranoside
SS2_22418094_2_1 531-538 abbr denotes Fuc-pNP
AE1_22418094_1_0 SS1_22418094_1_0 SS2_22418094_1_0 abbreviatedTo α-d-glucopyranosyl fluoride,GlcF
AE1_22418094_1_1 SS1_22418094_1_1 SS2_22418094_1_1 abbreviatedTo p-nitrophenyl,pNP
AE1_22418094_2_0 SS1_22418094_2_0 SS2_22418094_2_0 abbreviatedTo pNP-β-D-glucopyranoside,Glc-pNP
AE1_22418094_2_1 SS1_22418094_2_1 SS2_22418094_2_1 abbreviatedTo pNP-β-D-fucopyranoside,Fuc-pNP

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
TextSentencer_T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
TextSentencer_T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
TextSentencer_T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
TextSentencer_T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
TextSentencer_T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
TextSentencer_T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
TextSentencer_T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
TextSentencer_T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
TextSentencer_T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
TextSentencer_T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.
T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 339-344 Disease denotes cello http://purl.obolibrary.org/obo/MONDO_0013662

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 1657-1667 Glycan denotes cellobiose https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 339-344 Disease denotes cello http://purl.obolibrary.org/obo/MONDO_0013662

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 0-4 OrganismTaxon denotes Rice 4530
T2 117-121 OrganismTaxon denotes rice 4530
T3 1017-1021 OrganismTaxon denotes Rice 4530

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1089-1093 Body_part denotes back http://purl.obolibrary.org/obo/UBERON_0001137

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
TextSentencer_T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
TextSentencer_T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
TextSentencer_T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
TextSentencer_T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
TextSentencer_T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
TextSentencer_T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
TextSentencer_T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
TextSentencer_T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
TextSentencer_T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
TextSentencer_T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.
T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-112 Sentence denotes Rice BGlu1 glycosynthase and wild type transglycosylation activities distinguished by cyclophellitol inhibition.
T2 113-371 Sentence denotes The rice BGlu1 β-D-glucosidase nucleophile mutant E386G is a glycosynthase that catalyzes the synthesis of cellooligosaccharides from α-d-glucopyranosyl fluoride (GlcF) donor and p-nitrophenyl (pNP) cellobioside (Glc2-pNP) or cello-oligosaccharide acceptors.
T3 372-631 Sentence denotes When activity with other donors and acceptors was tested, the initial enzyme preparation cleaved pNP-β-D-glucopyranoside (Glc-pNP) and pNP-β-D-fucopyranoside (Fuc-pNP) to pNP and glucose and fucose, suggesting contamination with wild type BGlu1 β-glucosidase.
T4 632-837 Sentence denotes The products from reaction of GlcF and Fuc-pNP included Fuc-β-(1→3)-Fuc-pNP, Glc-β-(1→3)-Fuc-pNP, and Fuc-β-(1→4)-Glc-β-(1→3)-Fuc-pNP, suggesting the presence of both wild type BGlu1 and its glycosynthase.
T5 838-1016 Sentence denotes Inhibition of the BGlu1 β-glucosidase activity within this preparation by cyclophellitol confirmed that the E386G glycosynthase preparation was contaminated with wild type BGlu1.
T6 1017-1194 Sentence denotes Rice BGlu1 E386G-2, generated from a new construct designed to minimize back-mutation, showed glycosynthase activity without wild type hydrolytic or transglycosylation activity.
T7 1195-1402 Sentence denotes E386G-2 catalyzed transfer of glycosyl residues from GlcF, α-L-arabinosyl fluoride, α-D-fucosyl fluoride, α-D-galactosyl fluoride, α-D-mannosyl fluoride, and α-D-xylosyl fluoride donors to Glc2-pNP acceptor.
T8 1403-1574 Sentence denotes The synthetic products from the reactions of α-fucosyl fluoride and α-mannosyl fluoride donors were confirmed to result from addition of a β-(1→4)-linked glycosyl residue.
T9 1575-1790 Sentence denotes Moreover, the E386G glycosynthase transferred glucose from GlcF donor to glucose, cellobiose, Glc-pNP, Fuc-pNP, pNP-β-D-galactopyranoside, and pNP-β-D-xylopyranoside acceptors, but little to pNP-β-D-mannopyranoside.
T10 1791-1888 Sentence denotes Production of longer oligosaccharides occurred most readily on acceptors with an equatorial 4-OH.
T11 1889-2012 Sentence denotes Elimination of wild type contamination thereby allowed a clear assessment of BGlu1 E386G glycosynthase catalytic abilities.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 1657-1667 Glycan denotes cellobiose https://api.glycosmos.org/wurcs2image/latest/png/binary/G55560AP

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 0-4 OrganismTaxon denotes Rice 4530
T2 117-121 OrganismTaxon denotes rice 4530
T3 1017-1021 OrganismTaxon denotes Rice 4530

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1089-1093 Body_part denotes back http://purl.obolibrary.org/obo/UBERON_0001137