PubMed:2193097 JSONTXT

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":173,"end":189},"obj":"HP_0002721"}],"text":"Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression.\nLipopolysaccharide (LPS) potently stimulates human immunodeficiency virus type 1-long terminal repeat (HIV-1-LTR) CAT constructs transfected into monocyte/macrophage-like cell lines but not a T cell line. This effect appears to be mediated through the induction of nuclear factor kappa B (NF-kappa B). Electrophoretic mobility shift assays demonstrate that LPS induces a DNA binding activity indistinguishable from NF-kappa B in U937 and THP-1 cells. LPS is also shown to dramatically increase HIV-1 production from a chronically infected monocyte/macrophage-like cloned cell line, U1, which produces very low levels of HIV-1 at baseline. The stimulation of viral production from this cell line occurs only if these cells are treated with granulocyte/macrophage colony-stimulating factor (GM-CSF) before treatment with LPS. This stimulation of HIV-1 production is correlated with an increase in the level of HIV-1 RNA and and activation of NF-kappa B. LPS is not able to induce HIV-1 production in a cloned T cell line. The effect of LPS on HIV-1 replication occurs at picogram per milliliter concentrations and may be clinically significant in understanding the variability of the natural history of HIV-1 infection."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":167,"end":250},"obj":"DNA"},{"id":"T2","span":{"begin":268,"end":303},"obj":"cell_line"},{"id":"T3","span":{"begin":314,"end":325},"obj":"cell_line"},{"id":"T4","span":{"begin":387,"end":409},"obj":"protein"},{"id":"T5","span":{"begin":411,"end":421},"obj":"protein"},{"id":"T6","span":{"begin":537,"end":547},"obj":"protein"},{"id":"T7","span":{"begin":551,"end":555},"obj":"cell_line"},{"id":"T8","span":{"begin":560,"end":571},"obj":"cell_line"},{"id":"T9","span":{"begin":640,"end":702},"obj":"cell_line"},{"id":"T10","span":{"begin":704,"end":706},"obj":"cell_line"},{"id":"T11","span":{"begin":861,"end":909},"obj":"protein"},{"id":"T12","span":{"begin":911,"end":917},"obj":"protein"},{"id":"T13","span":{"begin":1030,"end":1039},"obj":"RNA"},{"id":"T14","span":{"begin":1062,"end":1072},"obj":"protein"},{"id":"T15","span":{"begin":1129,"end":1140},"obj":"cell_line"}],"text":"Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression.\nLipopolysaccharide (LPS) potently stimulates human immunodeficiency virus type 1-long terminal repeat (HIV-1-LTR) CAT constructs transfected into monocyte/macrophage-like cell lines but not a T cell line. This effect appears to be mediated through the induction of nuclear factor kappa B (NF-kappa B). Electrophoretic mobility shift assays demonstrate that LPS induces a DNA binding activity indistinguishable from NF-kappa B in U937 and THP-1 cells. LPS is also shown to dramatically increase HIV-1 production from a chronically infected monocyte/macrophage-like cloned cell line, U1, which produces very low levels of HIV-1 at baseline. The stimulation of viral production from this cell line occurs only if these cells are treated with granulocyte/macrophage colony-stimulating factor (GM-CSF) before treatment with LPS. This stimulation of HIV-1 production is correlated with an increase in the level of HIV-1 RNA and and activation of NF-kappa B. LPS is not able to induce HIV-1 production in a cloned T cell line. The effect of LPS on HIV-1 replication occurs at picogram per milliliter concentrations and may be clinically significant in understanding the variability of the natural history of HIV-1 infection."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":121},"obj":"Sentence"},{"id":"S2","span":{"begin":122,"end":326},"obj":"Sentence"},{"id":"S3","span":{"begin":327,"end":423},"obj":"Sentence"},{"id":"S4","span":{"begin":424,"end":572},"obj":"Sentence"},{"id":"S5","span":{"begin":573,"end":760},"obj":"Sentence"},{"id":"S6","span":{"begin":761,"end":945},"obj":"Sentence"},{"id":"S7","span":{"begin":946,"end":1073},"obj":"Sentence"},{"id":"S8","span":{"begin":1074,"end":1141},"obj":"Sentence"},{"id":"S9","span":{"begin":1142,"end":1339},"obj":"Sentence"}],"text":"Lipopolysaccharide is a potent monocyte/macrophage-specific stimulator of human immunodeficiency virus type 1 expression.\nLipopolysaccharide (LPS) potently stimulates human immunodeficiency virus type 1-long terminal repeat (HIV-1-LTR) CAT constructs transfected into monocyte/macrophage-like cell lines but not a T cell line. This effect appears to be mediated through the induction of nuclear factor kappa B (NF-kappa B). Electrophoretic mobility shift assays demonstrate that LPS induces a DNA binding activity indistinguishable from NF-kappa B in U937 and THP-1 cells. LPS is also shown to dramatically increase HIV-1 production from a chronically infected monocyte/macrophage-like cloned cell line, U1, which produces very low levels of HIV-1 at baseline. The stimulation of viral production from this cell line occurs only if these cells are treated with granulocyte/macrophage colony-stimulating factor (GM-CSF) before treatment with LPS. This stimulation of HIV-1 production is correlated with an increase in the level of HIV-1 RNA and and activation of NF-kappa B. LPS is not able to induce HIV-1 production in a cloned T cell line. The effect of LPS on HIV-1 replication occurs at picogram per milliliter concentrations and may be clinically significant in understanding the variability of the natural history of HIV-1 infection."}

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