PubMed:2191777 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":52},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":53,"end":176},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":177,"end":315},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":316,"end":366},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":367,"end":495},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":496,"end":568},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":569,"end":732},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":733,"end":824},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":825,"end":976},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":977,"end":1093},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1094,"end":1218},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1219,"end":1281},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":1282,"end":1472},"obj":"Sentence"},{"id":"TextSentencer_T14","span":{"begin":1473,"end":1601},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":52},"obj":"Sentence"},{"id":"T2","span":{"begin":53,"end":176},"obj":"Sentence"},{"id":"T3","span":{"begin":177,"end":315},"obj":"Sentence"},{"id":"T4","span":{"begin":316,"end":366},"obj":"Sentence"},{"id":"T5","span":{"begin":367,"end":495},"obj":"Sentence"},{"id":"T6","span":{"begin":496,"end":568},"obj":"Sentence"},{"id":"T7","span":{"begin":569,"end":732},"obj":"Sentence"},{"id":"T8","span":{"begin":733,"end":824},"obj":"Sentence"},{"id":"T9","span":{"begin":825,"end":976},"obj":"Sentence"},{"id":"T10","span":{"begin":977,"end":1093},"obj":"Sentence"},{"id":"T11","span":{"begin":1094,"end":1218},"obj":"Sentence"},{"id":"T12","span":{"begin":1219,"end":1281},"obj":"Sentence"},{"id":"T13","span":{"begin":1282,"end":1472},"obj":"Sentence"},{"id":"T14","span":{"begin":1473,"end":1601},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cell-wall glucans of Cryptococcus neoformans Cap 67.\nPurified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. Four 4-MMNO-soluble D-glucopyranans were isolated. Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. Gs-2 may have approximately 2% more chain branching than Gs-1. Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy)."}

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":169,"end":175},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":542,"end":548},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"Cell-wall glucans of Cryptococcus neoformans Cap 67.\nPurified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. Four 4-MMNO-soluble D-glucopyranans were isolated. Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. Gs-2 may have approximately 2% more chain branching than Gs-1. Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy)."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":21,"end":44},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":86,"end":98},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":620,"end":628},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"5207"},{"id":"A2","pred":"db_id","subj":"T2","obj":"5206"},{"id":"A3","pred":"db_id","subj":"T2","obj":"79213"},{"id":"A4","pred":"db_id","subj":"T4","obj":"124307"}],"text":"Cell-wall glucans of Cryptococcus neoformans Cap 67.\nPurified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. Four 4-MMNO-soluble D-glucopyranans were isolated. Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. Gs-2 may have approximately 2% more chain branching than Gs-1. Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy)."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":5,"end":9},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000060"}],"text":"Cell-wall glucans of Cryptococcus neoformans Cap 67.\nPurified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. Four 4-MMNO-soluble D-glucopyranans were isolated. Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. Gs-2 may have approximately 2% more chain branching than Gs-1. Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy)."}