| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-52 |
Sentence |
denotes |
Cell-wall glucans of Cryptococcus neoformans Cap 67. |
| TextSentencer_T2 |
53-176 |
Sentence |
denotes |
Purified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. |
| TextSentencer_T3 |
177-315 |
Sentence |
denotes |
The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. |
| TextSentencer_T4 |
316-366 |
Sentence |
denotes |
Four 4-MMNO-soluble D-glucopyranans were isolated. |
| TextSentencer_T5 |
367-495 |
Sentence |
denotes |
Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. |
| TextSentencer_T6 |
496-568 |
Sentence |
denotes |
A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. |
| TextSentencer_T7 |
569-732 |
Sentence |
denotes |
Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. |
| TextSentencer_T8 |
733-824 |
Sentence |
denotes |
Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. |
| TextSentencer_T9 |
825-976 |
Sentence |
denotes |
The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. |
| TextSentencer_T10 |
977-1093 |
Sentence |
denotes |
Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. |
| TextSentencer_T11 |
1094-1218 |
Sentence |
denotes |
Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. |
| TextSentencer_T12 |
1219-1281 |
Sentence |
denotes |
Gs-2 may have approximately 2% more chain branching than Gs-1. |
| TextSentencer_T13 |
1282-1472 |
Sentence |
denotes |
Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. |
| TextSentencer_T14 |
1473-1601 |
Sentence |
denotes |
Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy). |
| T1 |
0-52 |
Sentence |
denotes |
Cell-wall glucans of Cryptococcus neoformans Cap 67. |
| T2 |
53-176 |
Sentence |
denotes |
Purified cell walls derived from Cryptococcus neofromans Cap 67, an acapsular mutant, consisted of 86% Glc and 7.3% GlcNAc. |
| T3 |
177-315 |
Sentence |
denotes |
The integrity of the cell walls was disrupted in three successive extractions with 60% 4-methylmorpholine N-oxide (4-MMNO) at 120 degrees. |
| T4 |
316-366 |
Sentence |
denotes |
Four 4-MMNO-soluble D-glucopyranans were isolated. |
| T5 |
367-495 |
Sentence |
denotes |
Released within 0.5 h was water-insoluble Gi-1, followed by two water-soluble Gs fractions and water-insoluble Gi-2 over 17.5 h. |
| T6 |
496-568 |
Sentence |
denotes |
A 4-MMNO-insoluble residue, containing 27% of GlcNAc, was also isolated. |
| T7 |
569-732 |
Sentence |
denotes |
Gi-1 and Gi-2 were isolated as precipitates during dialysis of 4-MMNO extracts and were each reduced with NaBH4 to permit their investigation in alkaline solution. |
| T8 |
733-824 |
Sentence |
denotes |
Gs-1 and Gs-2 were separated by ion-exchange chromatography of the water-soluble fractions. |
| T9 |
825-976 |
Sentence |
denotes |
The structures of the D-glucopyranans were determined by 13C-n.m.r. spectroscopy and by g.l.c.-mass spectrometry of their per-O-methylated derivatives. |
| T10 |
977-1093 |
Sentence |
denotes |
Gi-1 was a (1----3)-alpha-D-glucopyranan (97%) with some (1----4)-D-glucosidic linkages (3%) and no chain-branching. |
| T11 |
1094-1218 |
Sentence |
denotes |
Gs-1 and Gs-2 were (1----6)-beta-D-glucopyranans branched at O-3 (10-12%) with beta-D-Glcp-(1----3)-beta-D-Glcp side chains. |
| T12 |
1219-1281 |
Sentence |
denotes |
Gs-2 may have approximately 2% more chain branching than Gs-1. |
| T13 |
1282-1472 |
Sentence |
denotes |
Gi-2 was a D-glucopyranan with 80% of its structure like that of Gi-1, and 20% like that of Gs-1 and -2; the water-insolubility of Gi-2 suggests that these structures were covalently linked. |
| T14 |
1473-1601 |
Sentence |
denotes |
Almost identical D-glucopyranans were obtained from aged cultures that had thickened walls (as observed by electron microscopy). |