PubMed:21833774 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/21833774","sourcedb":"PubMed","sourceid":"21833774","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/21833774","text":"Herbal formula HMC05 prevents human aortic smooth muscle cell migration and proliferation by inhibiting the ERK1/2 MAPK signaling cascade.\nHMC05 is a formulation derived from eight medicinal herbs, and prevents neointima formation by inhibition of the mitogen-activated protein kinase (MAPK) pathway with induction of heat shock protein 27 expression. In this study, we investigated the influence of HMC05 regulation on the MAPK/extracellular signal-regulated kinase (ERK) 1/2 signaling cascade in the inhibition of the migration and proliferation of human aortic smooth muscle cells (HASMCs). The inhibitory effects of HMC05 (25, 50, and 100 μg/ml) on tumor necrosis factor-alpha (TNF-α; 0 or 100 ng/ml)-induced HASMC migration and proliferation were investigated by wound migration and proliferation assays, Western blotting and reverse transcription-polymerase chain reaction. HMC05 completely inhibited TNF-α-induced HASMC migration and proliferation. HMC05 prevented TNF-α receptor 1-mediated phosphorylation of signal transduction molecules involved in MAPK signaling cascades such as MEK1/2, ERK1/2, Elk-1 transcription factor and p90 kDa ribosomal S6 kinase. The expression of matrix metalloproteinase, a modulator of vascular smooth muscle cell proliferation and migration, was inhibited by HMCO5 treatment, as was TNF-α-induced mRNA expression of intracellular adhesion molecule 1 and vascular cell adhesion molecule 1. HMC05 disruption of the MEK/ERK/Elk-1 and p90RSK pathways prevents HASMC migration and proliferation.","tracks":[{"project":"Allie","denotations":[{"id":"SS1_21833774_1_0","span":{"begin":252,"end":284},"obj":"expanded"},{"id":"SS2_21833774_1_0","span":{"begin":286,"end":290},"obj":"abbr"},{"id":"SS1_21833774_2_0","span":{"begin":429,"end":466},"obj":"expanded"},{"id":"SS2_21833774_2_0","span":{"begin":468,"end":471},"obj":"abbr"},{"id":"SS1_21833774_2_1","span":{"begin":551,"end":583},"obj":"expanded"},{"id":"SS2_21833774_2_1","span":{"begin":585,"end":591},"obj":"abbr"}],"relations":[{"id":"AE1_21833774_1_0","pred":"abbreviatedTo","subj":"SS1_21833774_1_0","obj":"SS2_21833774_1_0"},{"id":"AE1_21833774_2_0","pred":"abbreviatedTo","subj":"SS1_21833774_2_0","obj":"SS2_21833774_2_0"},{"id":"AE1_21833774_2_1","pred":"abbreviatedTo","subj":"SS1_21833774_2_1","obj":"SS2_21833774_2_1"}],"attributes":[{"subj":"SS1_21833774_1_0","pred":"source","obj":"Allie"},{"subj":"SS2_21833774_1_0","pred":"source","obj":"Allie"},{"subj":"SS1_21833774_2_0","pred":"source","obj":"Allie"},{"subj":"SS2_21833774_2_0","pred":"source","obj":"Allie"},{"subj":"SS1_21833774_2_1","pred":"source","obj":"Allie"},{"subj":"SS2_21833774_2_1","pred":"source","obj":"Allie"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":653,"end":658},"obj":"HP_0002664"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"PubMed_ArguminSci","denotations":[{"id":"T1","span":{"begin":139,"end":351},"obj":"DRI_Background"},{"id":"T2","span":{"begin":352,"end":423},"obj":"DRI_Challenge"},{"id":"T3","span":{"begin":460,"end":593},"obj":"DRI_Challenge"},{"id":"T4","span":{"begin":956,"end":1166},"obj":"DRI_Background"},{"id":"T5","span":{"begin":1167,"end":1429},"obj":"DRI_Background"},{"id":"T6","span":{"begin":1430,"end":1531},"obj":"DRI_Approach"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T2","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T3","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T4","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T5","pred":"source","obj":"PubMed_ArguminSci"},{"subj":"T6","pred":"source","obj":"PubMed_ArguminSci"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Allie","color":"#daec93","default":true},{"id":"PubmedHPO","color":"#e393ec"},{"id":"PubMed_ArguminSci","color":"#93ecc9"}]}]}}