PubMed:2163347 JSON TXT

Annnotations TAB JSON ListView MergeView

jnlpba-st-training

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":37,"end":54},"obj":"protein"},{"id":"T2","span":{"begin":55,"end":60},"obj":"protein"},{"id":"T3","span":{"begin":71,"end":105},"obj":"DNA"},{"id":"T4","span":{"begin":117,"end":137},"obj":"DNA"},{"id":"T5","span":{"begin":179,"end":204},"obj":"DNA"},{"id":"T6","span":{"begin":219,"end":241},"obj":"DNA"},{"id":"T7","span":{"begin":299,"end":316},"obj":"protein"},{"id":"T8","span":{"begin":386,"end":399},"obj":"cell_type"},{"id":"T9","span":{"begin":441,"end":456},"obj":"DNA"},{"id":"T10","span":{"begin":467,"end":504},"obj":"protein"},{"id":"T11","span":{"begin":508,"end":530},"obj":"DNA"},{"id":"T12","span":{"begin":603,"end":640},"obj":"DNA"},{"id":"T13","span":{"begin":702,"end":722},"obj":"DNA"},{"id":"T14","span":{"begin":724,"end":727},"obj":"DNA"},{"id":"T15","span":{"begin":734,"end":747},"obj":"DNA"},{"id":"T16","span":{"begin":755,"end":780},"obj":"DNA"},{"id":"T17","span":{"begin":782,"end":785},"obj":"DNA"},{"id":"T18","span":{"begin":849,"end":881},"obj":"protein"},{"id":"T19","span":{"begin":889,"end":908},"obj":"protein"},{"id":"T20","span":{"begin":914,"end":917},"obj":"DNA"},{"id":"T21","span":{"begin":953,"end":974},"obj":"protein"},{"id":"T22","span":{"begin":1113,"end":1123},"obj":"DNA"},{"id":"T23","span":{"begin":1171,"end":1189},"obj":"DNA"},{"id":"T24","span":{"begin":1288,"end":1304},"obj":"DNA"},{"id":"T25","span":{"begin":1331,"end":1349},"obj":"DNA"},{"id":"T26","span":{"begin":1384,"end":1402},"obj":"DNA"},{"id":"T27","span":{"begin":1406,"end":1425},"obj":"cell_type"},{"id":"T28","span":{"begin":1486,"end":1499},"obj":"DNA"},{"id":"T29","span":{"begin":1530,"end":1535},"obj":"protein"},{"id":"T30","span":{"begin":1629,"end":1646},"obj":"DNA"},{"id":"T31","span":{"begin":1673,"end":1704},"obj":"protein"}],"text":"Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.\nThe ets proto-oncogene family is a group of sequence-related genes whose normal cellular function is unknown. In a study of cellular proteins involved in the transcriptional regulation of murine retroviruses in T lymphocytes, we have discovered that a member of the ets gene family encodes a sequence-specific DNA-binding protein. A mouse ets-1 cDNA clone was obtained by screening a mouse thymus cDNA expression library with a double-stranded oligonucleotide probe representing 20 bp of the Moloney murine sarcoma virus (MSV) long terminal repeat (LTR). The cDNA sequence has an 813-bp open reading frame (ORF) whose predicted amino acid sequence is 97.6% identical to the 272 carboxy-terminal amino acids of the human ets-1 protein. The ORF was expressed in bacteria, and the 30-kD protein product was shown to bind DNA in a sequence-specific manner by mobility-shift assays, Southwestern blot analysis, and methylation interference. A mutant LTR containing four base pair substitutions in the ets-1 binding site was constructed and was shown to have reduced binding in vitro. Transcriptional efficiency of the MSV LTR promoter containing this disrupted ets-1 binding site was compared to the activity of a wild-type promoter in mouse T lymphocytes in culture, and 15- to 20-fold reduction in expression of a reporter gene was observed. We propose that ets-1 functions as a transcriptional activator of mammalian type-C retroviruses and speculate that ets-related genes constitute a new group of eukaryotic DNA-binding proteins."}

PubmedHPO

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":682,"end":689},"obj":"HP_0100242"}],"text":"Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.\nThe ets proto-oncogene family is a group of sequence-related genes whose normal cellular function is unknown. In a study of cellular proteins involved in the transcriptional regulation of murine retroviruses in T lymphocytes, we have discovered that a member of the ets gene family encodes a sequence-specific DNA-binding protein. A mouse ets-1 cDNA clone was obtained by screening a mouse thymus cDNA expression library with a double-stranded oligonucleotide probe representing 20 bp of the Moloney murine sarcoma virus (MSV) long terminal repeat (LTR). The cDNA sequence has an 813-bp open reading frame (ORF) whose predicted amino acid sequence is 97.6% identical to the 272 carboxy-terminal amino acids of the human ets-1 protein. The ORF was expressed in bacteria, and the 30-kD protein product was shown to bind DNA in a sequence-specific manner by mobility-shift assays, Southwestern blot analysis, and methylation interference. A mutant LTR containing four base pair substitutions in the ets-1 binding site was constructed and was shown to have reduced binding in vitro. Transcriptional efficiency of the MSV LTR promoter containing this disrupted ets-1 binding site was compared to the activity of a wild-type promoter in mouse T lymphocytes in culture, and 15- to 20-fold reduction in expression of a reporter gene was observed. We propose that ets-1 functions as a transcriptional activator of mammalian type-C retroviruses and speculate that ets-related genes constitute a new group of eukaryotic DNA-binding proteins."}

pubmed-sentences-benchmark

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":174},"obj":"Sentence"},{"id":"S2","span":{"begin":175,"end":284},"obj":"Sentence"},{"id":"S3","span":{"begin":285,"end":505},"obj":"Sentence"},{"id":"S4","span":{"begin":506,"end":729},"obj":"Sentence"},{"id":"S5","span":{"begin":730,"end":909},"obj":"Sentence"},{"id":"S6","span":{"begin":910,"end":1110},"obj":"Sentence"},{"id":"S7","span":{"begin":1111,"end":1253},"obj":"Sentence"},{"id":"S8","span":{"begin":1254,"end":1513},"obj":"Sentence"},{"id":"S9","span":{"begin":1514,"end":1705},"obj":"Sentence"}],"text":"Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.\nThe ets proto-oncogene family is a group of sequence-related genes whose normal cellular function is unknown. In a study of cellular proteins involved in the transcriptional regulation of murine retroviruses in T lymphocytes, we have discovered that a member of the ets gene family encodes a sequence-specific DNA-binding protein. A mouse ets-1 cDNA clone was obtained by screening a mouse thymus cDNA expression library with a double-stranded oligonucleotide probe representing 20 bp of the Moloney murine sarcoma virus (MSV) long terminal repeat (LTR). The cDNA sequence has an 813-bp open reading frame (ORF) whose predicted amino acid sequence is 97.6% identical to the 272 carboxy-terminal amino acids of the human ets-1 protein. The ORF was expressed in bacteria, and the 30-kD protein product was shown to bind DNA in a sequence-specific manner by mobility-shift assays, Southwestern blot analysis, and methylation interference. A mutant LTR containing four base pair substitutions in the ets-1 binding site was constructed and was shown to have reduced binding in vitro. Transcriptional efficiency of the MSV LTR promoter containing this disrupted ets-1 binding site was compared to the activity of a wild-type promoter in mouse T lymphocytes in culture, and 15- to 20-fold reduction in expression of a reporter gene was observed. We propose that ets-1 functions as a transcriptional activator of mammalian type-C retroviruses and speculate that ets-related genes constitute a new group of eukaryotic DNA-binding proteins."}

genia-medco-coref

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":113,"end":173},"obj":"NP"},{"id":"C2","span":{"begin":208,"end":241},"obj":"NP"},{"id":"C3","span":{"begin":242,"end":247},"obj":"NP"},{"id":"C4","span":{"begin":663,"end":728},"obj":"NP"},{"id":"C5","span":{"begin":752,"end":786},"obj":"NP"},{"id":"C6","span":{"begin":787,"end":792},"obj":"NP"},{"id":"C7","span":{"begin":910,"end":917},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-relat","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C1"},{"id":"R3","pred":"coref-relat","subj":"C6","obj":"C5"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C5"}],"text":"Sequence-specific DNA binding of the proto-oncoprotein ets-1 defines a transcriptional activator sequence within the long terminal repeat of the Moloney murine sarcoma virus.\nThe ets proto-oncogene family is a group of sequence-related genes whose normal cellular function is unknown. In a study of cellular proteins involved in the transcriptional regulation of murine retroviruses in T lymphocytes, we have discovered that a member of the ets gene family encodes a sequence-specific DNA-binding protein. A mouse ets-1 cDNA clone was obtained by screening a mouse thymus cDNA expression library with a double-stranded oligonucleotide probe representing 20 bp of the Moloney murine sarcoma virus (MSV) long terminal repeat (LTR). The cDNA sequence has an 813-bp open reading frame (ORF) whose predicted amino acid sequence is 97.6% identical to the 272 carboxy-terminal amino acids of the human ets-1 protein. The ORF was expressed in bacteria, and the 30-kD protein product was shown to bind DNA in a sequence-specific manner by mobility-shift assays, Southwestern blot analysis, and methylation interference. A mutant LTR containing four base pair substitutions in the ets-1 binding site was constructed and was shown to have reduced binding in vitro. Transcriptional efficiency of the MSV LTR promoter containing this disrupted ets-1 binding site was compared to the activity of a wild-type promoter in mouse T lymphocytes in culture, and 15- to 20-fold reduction in expression of a reporter gene was observed. We propose that ets-1 functions as a transcriptional activator of mammalian type-C retroviruses and speculate that ets-related genes constitute a new group of eukaryotic DNA-binding proteins."}

GENIAcorpus