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Id Subject Object Predicate Lexical cue
T1 0-69 Sentence denotes Affinity labeling of spinach leaf phosphoribulokinase by ATP analogs.
T2 70-108 Sentence denotes Modification of an active site lysine.
T3 109-218 Sentence denotes Spinach leaf phosphoribulokinase is sensitive to modification by ATP analogs that react with lysine residues.
T4 219-318 Sentence denotes The 2',3'-dialdehyde derivative of ATP (oATP) inactivates enzyme in a slow, time-dependent fashion.
T5 319-484 Sentence denotes The process follows first-order kinetics (kinact = 0.07 min-1), and the concentration dependence of inactivation indicates tight inhibitor binding (Ki = 106 microM).
T6 485-610 Sentence denotes ATP offers good protection against inactivation (Kd = 67 microM), suggesting that oATP is directed toward the catalytic site.
T7 611-713 Sentence denotes This conclusion is supported by the fact that oATP functions as an alternate substrate (Km = 0.55 mM).
T8 714-815 Sentence denotes Inactivation of phosphoribulokinase by [14C]oATP results in a modification stoichiometry of 0.7/site.
T9 816-960 Sentence denotes The 14C-labeled enzyme is stable to dialysis, suggesting that the covalent adduct formed between protein and oATP is not a simple Schiff's base.
T10 961-1109 Sentence denotes Adenosine di- and triphosphopyridoxals (Ado-P2-Pl and Ado-P3-Pl, respectively) also inhibit spinach phosphoribulokinase in a time-dependent fashion.
T11 1110-1299 Sentence denotes In this case, activity loss is reversible unless the inhibited species is borohydride-reduced, suggesting that Ado-P2-Pl and Ado-P3-Pl form Schiff's bases with an amino group on the enzyme.
T12 1300-1398 Sentence denotes Protection is afforded by the substrate ATP, suggesting that modification is active site-directed.
T13 1399-1516 Sentence denotes Prolonged incubation of enzyme with these inhibitors does not result in complete inactivation of phosphoribulokinase.
T14 1517-1708 Sentence denotes Residual activity is dependent on inhibitor concentration, as would be expected if equilibrium is established between the noncovalent E.I complex and the covalent (Schiff's base) E-I species.
T15 1709-1814 Sentence denotes Kinetic data analysis indicates Ki values of 175 and 11 microM for Ado-P2-Pl and Ado-P3-Pl, respectively.
T16 1815-1933 Sentence denotes Thus, the ATP-binding domain can easily accommodate the pyridoxal moiety which is tethered to the polyphosphate chain.
T17 1934-2217 Sentence denotes The phosphorylated ATP analogs employed in this study exhibit substantially tighter binding to phosphoribulokinase than does fluorosulfonyl-benzoyladenosine (Ki = 4.8 mM), which we have previously demonstrated to be useful in selectively modifying the ATP-binding domain (Krieger, T.
T18 2218-2238 Sentence denotes J., and Miziorko, H.
T19 2239-2288 Sentence denotes M. (1986) Biochemistry 25, 3496-3501; Krieger, T.
T20 2289-2310 Sentence denotes J., Mende-Mueller, L.
T21 2311-2331 Sentence denotes M., and Miziorko, H.
T22 2332-2350 Sentence denotes M. (1987) Biochim.
T23 2351-2359 Sentence denotes Biophys.
T24 2360-2379 Sentence denotes Acta 915, 112-119).
T25 2380-2630 Sentence denotes Although the adduct formed between oATP and enzyme was unsuitable for structural analysis, borohydride reduction of the Schiff's base formed between enzyme and Ado-P3-[3H]Pl produced a species useful for investigation by protein chemistry techniques.
T26 2631-2775 Sentence denotes A radiolabeled tryptic peptide was prepared, isolated, and sequenced; the data indicate that lysine 68 is the residue modified by Ado-P3-[3H]Pl.
T1 0-69 Sentence denotes Affinity labeling of spinach leaf phosphoribulokinase by ATP analogs.
T2 70-108 Sentence denotes Modification of an active site lysine.
T3 109-218 Sentence denotes Spinach leaf phosphoribulokinase is sensitive to modification by ATP analogs that react with lysine residues.
T4 219-318 Sentence denotes The 2',3'-dialdehyde derivative of ATP (oATP) inactivates enzyme in a slow, time-dependent fashion.
T5 319-484 Sentence denotes The process follows first-order kinetics (kinact = 0.07 min-1), and the concentration dependence of inactivation indicates tight inhibitor binding (Ki = 106 microM).
T6 485-610 Sentence denotes ATP offers good protection against inactivation (Kd = 67 microM), suggesting that oATP is directed toward the catalytic site.
T7 611-713 Sentence denotes This conclusion is supported by the fact that oATP functions as an alternate substrate (Km = 0.55 mM).
T8 714-815 Sentence denotes Inactivation of phosphoribulokinase by [14C]oATP results in a modification stoichiometry of 0.7/site.
T9 816-960 Sentence denotes The 14C-labeled enzyme is stable to dialysis, suggesting that the covalent adduct formed between protein and oATP is not a simple Schiff's base.
T10 961-1109 Sentence denotes Adenosine di- and triphosphopyridoxals (Ado-P2-Pl and Ado-P3-Pl, respectively) also inhibit spinach phosphoribulokinase in a time-dependent fashion.
T11 1110-1299 Sentence denotes In this case, activity loss is reversible unless the inhibited species is borohydride-reduced, suggesting that Ado-P2-Pl and Ado-P3-Pl form Schiff's bases with an amino group on the enzyme.
T12 1300-1398 Sentence denotes Protection is afforded by the substrate ATP, suggesting that modification is active site-directed.
T13 1399-1516 Sentence denotes Prolonged incubation of enzyme with these inhibitors does not result in complete inactivation of phosphoribulokinase.
T14 1517-1708 Sentence denotes Residual activity is dependent on inhibitor concentration, as would be expected if equilibrium is established between the noncovalent E.I complex and the covalent (Schiff's base) E-I species.
T15 1709-1814 Sentence denotes Kinetic data analysis indicates Ki values of 175 and 11 microM for Ado-P2-Pl and Ado-P3-Pl, respectively.
T16 1815-1933 Sentence denotes Thus, the ATP-binding domain can easily accommodate the pyridoxal moiety which is tethered to the polyphosphate chain.
T17 1934-2217 Sentence denotes The phosphorylated ATP analogs employed in this study exhibit substantially tighter binding to phosphoribulokinase than does fluorosulfonyl-benzoyladenosine (Ki = 4.8 mM), which we have previously demonstrated to be useful in selectively modifying the ATP-binding domain (Krieger, T.
T18 2218-2238 Sentence denotes J., and Miziorko, H.
T19 2239-2288 Sentence denotes M. (1986) Biochemistry 25, 3496-3501; Krieger, T.
T20 2289-2310 Sentence denotes J., Mende-Mueller, L.
T21 2311-2331 Sentence denotes M., and Miziorko, H.
T22 2332-2350 Sentence denotes M. (1987) Biochim.
T23 2351-2359 Sentence denotes Biophys.
T24 2360-2379 Sentence denotes Acta 915, 112-119).
T25 2380-2630 Sentence denotes Although the adduct formed between oATP and enzyme was unsuitable for structural analysis, borohydride reduction of the Schiff's base formed between enzyme and Ado-P3-[3H]Pl produced a species useful for investigation by protein chemistry techniques.
T26 2631-2775 Sentence denotes A radiolabeled tryptic peptide was prepared, isolated, and sequenced; the data indicate that lysine 68 is the residue modified by Ado-P3-[3H]Pl.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 852-860 OrganismTaxon denotes dialysis 124307