PubMed:21364582 JSON TXT

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DisGeNET

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":1297,"end":1301},"obj":"gene:1825"},{"id":"T1","span":{"begin":1305,"end":1308},"obj":"disease:C0009402"},{"id":"T2","span":{"begin":1297,"end":1301},"obj":"gene:1825"},{"id":"T3","span":{"begin":1305,"end":1308},"obj":"disease:C1527249"},{"id":"T4","span":{"begin":1297,"end":1301},"obj":"gene:1824"},{"id":"T5","span":{"begin":1305,"end":1308},"obj":"disease:C0009402"},{"id":"T6","span":{"begin":1297,"end":1301},"obj":"gene:1824"},{"id":"T7","span":{"begin":1305,"end":1308},"obj":"disease:C1527249"},{"id":"T8","span":{"begin":1343,"end":1347},"obj":"gene:1825"},{"id":"T9","span":{"begin":1379,"end":1382},"obj":"disease:C0009402"},{"id":"T10","span":{"begin":1343,"end":1347},"obj":"gene:1825"},{"id":"T11","span":{"begin":1379,"end":1382},"obj":"disease:C1527249"},{"id":"T12","span":{"begin":1343,"end":1347},"obj":"gene:1824"},{"id":"T13","span":{"begin":1379,"end":1382},"obj":"disease:C0009402"},{"id":"T14","span":{"begin":1343,"end":1347},"obj":"gene:1824"},{"id":"T15","span":{"begin":1379,"end":1382},"obj":"disease:C1527249"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"},{"id":"R6","pred":"associated_with","subj":"T10","obj":"T11"},{"id":"R7","pred":"associated_with","subj":"T12","obj":"T13"},{"id":"R8","pred":"associated_with","subj":"T14","obj":"T15"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"DSC3 expression is regulated by p53, and methylation of DSC3 DNA is a prognostic marker in human colorectal cancer.\nBACKGROUND: Desmocollin 3 (DSC3), a member of the cadherin superfamily and integral component of desmosomes, is involved in carcinogenesis. However, the role of DSC3 in colorectal cancer (CRC) has not yet been established.\nMETHODS: Desmocollin 3 expression in CRC cell lines was analysed by RT-PCR and western blotting. Methylation status of DSC3 was examined by demethylation tests, methylation-specific PCR, and bisulphite sequencing (BS). The regulatory role of p53 was investigated by transfection.\nRESULTS: Desmocollin 3 was downregulated in CRC cells at mRNA and protein levels. Desmocollin 3 expression was restored in five out of seven cell lines after 5-aza-2'-deoxycytidine (DAC) treatment. A heterogeneous methylation pattern was detected by BS in promoter region and exon 1 of DSC3. Methylation of DSC3 genomic sequences was found in 41% (41 out of 99) of primary CRC, being associated with poor prognosis (P=0.002). Transfection of p53 alone or in combination of DAC increased the DSC3 expression. Similarly, treatment with p53 inducer adriamycin alone or in combination with DAC enhanced DSC3 expression.\nCONCLUSIONS: DNA methylation contributes to downregulation of DSC3 in CRC cell lines. Methylation status of DSC3 DNA is a prognostic marker for CRC. P53 appears to have an important role in regulating DSC3 expression."}

Allie

{"project":"Allie","denotations":[{"id":"SS1_21364582_2_0","span":{"begin":128,"end":141},"obj":"expanded"},{"id":"SS2_21364582_2_0","span":{"begin":143,"end":147},"obj":"abbr"},{"id":"SS1_21364582_3_0","span":{"begin":285,"end":302},"obj":"expanded"},{"id":"SS2_21364582_3_0","span":{"begin":304,"end":307},"obj":"abbr"},{"id":"SS1_21364582_6_0","span":{"begin":530,"end":551},"obj":"expanded"},{"id":"SS2_21364582_6_0","span":{"begin":553,"end":555},"obj":"abbr"},{"id":"SS1_21364582_10_0","span":{"begin":777,"end":799},"obj":"expanded"},{"id":"SS2_21364582_10_0","span":{"begin":801,"end":804},"obj":"abbr"}],"relations":[{"id":"AE1_21364582_2_0","pred":"abbreviatedTo","subj":"SS1_21364582_2_0","obj":"SS2_21364582_2_0"},{"id":"AE1_21364582_3_0","pred":"abbreviatedTo","subj":"SS1_21364582_3_0","obj":"SS2_21364582_3_0"},{"id":"AE1_21364582_6_0","pred":"abbreviatedTo","subj":"SS1_21364582_6_0","obj":"SS2_21364582_6_0"},{"id":"AE1_21364582_10_0","pred":"abbreviatedTo","subj":"SS1_21364582_10_0","obj":"SS2_21364582_10_0"}],"text":"DSC3 expression is regulated by p53, and methylation of DSC3 DNA is a prognostic marker in human colorectal cancer.\nBACKGROUND: Desmocollin 3 (DSC3), a member of the cadherin superfamily and integral component of desmosomes, is involved in carcinogenesis. However, the role of DSC3 in colorectal cancer (CRC) has not yet been established.\nMETHODS: Desmocollin 3 expression in CRC cell lines was analysed by RT-PCR and western blotting. Methylation status of DSC3 was examined by demethylation tests, methylation-specific PCR, and bisulphite sequencing (BS). The regulatory role of p53 was investigated by transfection.\nRESULTS: Desmocollin 3 was downregulated in CRC cells at mRNA and protein levels. Desmocollin 3 expression was restored in five out of seven cell lines after 5-aza-2'-deoxycytidine (DAC) treatment. A heterogeneous methylation pattern was detected by BS in promoter region and exon 1 of DSC3. Methylation of DSC3 genomic sequences was found in 41% (41 out of 99) of primary CRC, being associated with poor prognosis (P=0.002). Transfection of p53 alone or in combination of DAC increased the DSC3 expression. Similarly, treatment with p53 inducer adriamycin alone or in combination with DAC enhanced DSC3 expression.\nCONCLUSIONS: DNA methylation contributes to downregulation of DSC3 in CRC cell lines. Methylation status of DSC3 DNA is a prognostic marker for CRC. P53 appears to have an important role in regulating DSC3 expression."}

PubmedHPO

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":296,"end":302},"obj":"HP_0002664"}],"text":"DSC3 expression is regulated by p53, and methylation of DSC3 DNA is a prognostic marker in human colorectal cancer.\nBACKGROUND: Desmocollin 3 (DSC3), a member of the cadherin superfamily and integral component of desmosomes, is involved in carcinogenesis. However, the role of DSC3 in colorectal cancer (CRC) has not yet been established.\nMETHODS: Desmocollin 3 expression in CRC cell lines was analysed by RT-PCR and western blotting. Methylation status of DSC3 was examined by demethylation tests, methylation-specific PCR, and bisulphite sequencing (BS). The regulatory role of p53 was investigated by transfection.\nRESULTS: Desmocollin 3 was downregulated in CRC cells at mRNA and protein levels. Desmocollin 3 expression was restored in five out of seven cell lines after 5-aza-2'-deoxycytidine (DAC) treatment. A heterogeneous methylation pattern was detected by BS in promoter region and exon 1 of DSC3. Methylation of DSC3 genomic sequences was found in 41% (41 out of 99) of primary CRC, being associated with poor prognosis (P=0.002). Transfection of p53 alone or in combination of DAC increased the DSC3 expression. Similarly, treatment with p53 inducer adriamycin alone or in combination with DAC enhanced DSC3 expression.\nCONCLUSIONS: DNA methylation contributes to downregulation of DSC3 in CRC cell lines. Methylation status of DSC3 DNA is a prognostic marker for CRC. P53 appears to have an important role in regulating DSC3 expression."}

DisGeNET5_gene_disease

DisGeNet-2017-sample

{"project":"DisGeNet-2017-sample","denotations":[{"id":"T1960","span":{"begin":0,"end":4},"obj":"gene:1825"},{"id":"T1961","span":{"begin":97,"end":114},"obj":"disease:C0009402"},{"id":"T1962","span":{"begin":56,"end":60},"obj":"gene:1825"},{"id":"T1963","span":{"begin":128,"end":141},"obj":"gene:1825"},{"id":"T1964","span":{"begin":240,"end":254},"obj":"disease:C0596263"},{"id":"T1965","span":{"begin":628,"end":641},"obj":"gene:1824"},{"id":"T1966","span":{"begin":663,"end":666},"obj":"disease:C0009402"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T1960","obj":"T1961"},{"id":"R2","pred":"associated_with","subj":"T1960","obj":"T1961"},{"id":"R3","pred":"associated_with","subj":"T1962","obj":"T1961"},{"id":"R4","pred":"associated_with","subj":"T1962","obj":"T1961"},{"id":"R5","pred":"associated_with","subj":"T1963","obj":"T1964"},{"id":"R6","pred":"associated_with","subj":"T1963","obj":"T1964"},{"id":"R7","pred":"associated_with","subj":"T1965","obj":"T1966"},{"id":"R8","pred":"associated_with","subj":"T1965","obj":"T1966"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"DSC3 expression is regulated by p53, and methylation of DSC3 DNA is a prognostic marker in human colorectal cancer.\nBACKGROUND: Desmocollin 3 (DSC3), a member of the cadherin superfamily and integral component of desmosomes, is involved in carcinogenesis. However, the role of DSC3 in colorectal cancer (CRC) has not yet been established.\nMETHODS: Desmocollin 3 expression in CRC cell lines was analysed by RT-PCR and western blotting. Methylation status of DSC3 was examined by demethylation tests, methylation-specific PCR, and bisulphite sequencing (BS). The regulatory role of p53 was investigated by transfection.\nRESULTS: Desmocollin 3 was downregulated in CRC cells at mRNA and protein levels. Desmocollin 3 expression was restored in five out of seven cell lines after 5-aza-2'-deoxycytidine (DAC) treatment. A heterogeneous methylation pattern was detected by BS in promoter region and exon 1 of DSC3. Methylation of DSC3 genomic sequences was found in 41% (41 out of 99) of primary CRC, being associated with poor prognosis (P=0.002). Transfection of p53 alone or in combination of DAC increased the DSC3 expression. Similarly, treatment with p53 inducer adriamycin alone or in combination with DAC enhanced DSC3 expression.\nCONCLUSIONS: DNA methylation contributes to downregulation of DSC3 in CRC cell lines. Methylation status of DSC3 DNA is a prognostic marker for CRC. P53 appears to have an important role in regulating DSC3 expression."}