PubMed:2123468
Annnotations
jnlpba-st-training
{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":23,"end":43},"obj":"protein"},{"id":"T2","span":{"begin":121,"end":144},"obj":"protein"},{"id":"T3","span":{"begin":161,"end":174},"obj":"cell_type"},{"id":"T4","span":{"begin":189,"end":205},"obj":"protein"},{"id":"T5","span":{"begin":241,"end":262},"obj":"protein"},{"id":"T6","span":{"begin":274,"end":279},"obj":"protein"},{"id":"T7","span":{"begin":284,"end":294},"obj":"protein"},{"id":"T8","span":{"begin":412,"end":432},"obj":"protein"},{"id":"T9","span":{"begin":436,"end":459},"obj":"cell_type"},{"id":"T10","span":{"begin":549,"end":567},"obj":"protein"},{"id":"T11","span":{"begin":569,"end":577},"obj":"protein"},{"id":"T12","span":{"begin":617,"end":622},"obj":"protein"},{"id":"T13","span":{"begin":654,"end":661},"obj":"cell_type"},{"id":"T14","span":{"begin":741,"end":759},"obj":"DNA"},{"id":"T15","span":{"begin":789,"end":798},"obj":"DNA"},{"id":"T16","span":{"begin":829,"end":869},"obj":"cell_line"},{"id":"T17","span":{"begin":951,"end":959},"obj":"protein"},{"id":"T18","span":{"begin":1113,"end":1121},"obj":"protein"},{"id":"T19","span":{"begin":1150,"end":1155},"obj":"protein"},{"id":"T20","span":{"begin":1209,"end":1214},"obj":"protein"},{"id":"T21","span":{"begin":1315,"end":1320},"obj":"protein"},{"id":"T22","span":{"begin":1357,"end":1380},"obj":"protein"},{"id":"T23","span":{"begin":1388,"end":1396},"obj":"DNA"},{"id":"T24","span":{"begin":1431,"end":1441},"obj":"protein"},{"id":"T25","span":{"begin":1456,"end":1478},"obj":"DNA"},{"id":"T26","span":{"begin":1585,"end":1606},"obj":"protein"},{"id":"T27","span":{"begin":1635,"end":1650},"obj":"DNA"}],"text":"Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.\nStimulation of T lymphocytes through their antigen receptor leads to the appearance of several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate the activity of a single transcription factor in individual viable cells, we have applied an assay that uses the fluorescence-activated cell sorter to quantitate beta-galactosidase (beta-gal). We have analyzed the distribution of NF-AT transcriptional activity among T cells undergoing activation by using a construct in which three tandem copies of the NF-AT-binding site directs transcription of the lacZ gene. Unexpectedly, stimulation of cloned stably transfected Jurkat T cells leads to a bimodal pattern of beta-gal expression in which some cells express no beta-gal and others express high levels. This expression pattern cannot be accounted for by cell-cycle position or heritable variation. Further results, in which beta-gal activity is correlated with NF-AT-binding activity, indicate that the concentration of NF-AT must exceed a critical threshold before transcription initiates. This threshold likely reflects the NF-AT concentration-dependent assembly of transcription complexes at the promoter. Similar constructs controlled by NF-kappa B or the entire interleukin-2 enhancer show bimodal expression patterns during induction, suggesting that thresholds set by the concentration of transcription factors may be a common property of inducible genes."}
pubmed-sentences-benchmark
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genia-medco-coref
{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":117,"end":144},"obj":"NP"},{"id":"C2","span":{"begin":161,"end":174},"obj":"NP"},{"id":"C4","span":{"begin":183,"end":188},"obj":"NP"},{"id":"C3","span":{"begin":183,"end":205},"obj":"NP"},{"id":"C5","span":{"begin":233,"end":262},"obj":"NP"},{"id":"C6","span":{"begin":284,"end":294},"obj":"NP"},{"id":"C7","span":{"begin":296,"end":301},"obj":"NP"},{"id":"C8","span":{"begin":477,"end":485},"obj":"NP"},{"id":"C9","span":{"begin":486,"end":490},"obj":"NP"},{"id":"C10","span":{"begin":549,"end":578},"obj":"NP"},{"id":"C11","span":{"begin":693,"end":704},"obj":"NP"},{"id":"C12","span":{"begin":708,"end":713},"obj":"NP"},{"id":"C14","span":{"begin":879,"end":919},"obj":"NP"},{"id":"C15","span":{"begin":923,"end":928},"obj":"NP"},{"id":"C16","span":{"begin":951,"end":959},"obj":"NP"},{"id":"C13","span":{"begin":879,"end":990},"obj":"NP"},{"id":"C17","span":{"begin":992,"end":1015},"obj":"NP"},{"id":"C18","span":{"begin":1227,"end":1247},"obj":"NP"},{"id":"C19","span":{"begin":1280,"end":1294},"obj":"NP"},{"id":"C20","span":{"begin":1431,"end":1441},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-pron","subj":"C4","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C3","obj":"C1"},{"id":"R3","pred":"coref-relat","subj":"C7","obj":"C5"},{"id":"R4","pred":"coref-relat","subj":"C9","obj":"C8"},{"id":"R5","pred":"coref-relat","subj":"C12","obj":"C11"},{"id":"R6","pred":"coref-relat","subj":"C15","obj":"C13"},{"id":"R7","pred":"coref-ident","subj":"C16","obj":"C10"},{"id":"R8","pred":"coref-ident","subj":"C17","obj":"C14"},{"id":"R9","pred":"coref-ident","subj":"C19","obj":"C18"},{"id":"R10","pred":"coref-ident","subj":"C20","obj":"C6"}],"text":"Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.\nStimulation of T lymphocytes through their antigen receptor leads to the appearance of several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate the activity of a single transcription factor in individual viable cells, we have applied an assay that uses the fluorescence-activated cell sorter to quantitate beta-galactosidase (beta-gal). We have analyzed the distribution of NF-AT transcriptional activity among T cells undergoing activation by using a construct in which three tandem copies of the NF-AT-binding site directs transcription of the lacZ gene. Unexpectedly, stimulation of cloned stably transfected Jurkat T cells leads to a bimodal pattern of beta-gal expression in which some cells express no beta-gal and others express high levels. This expression pattern cannot be accounted for by cell-cycle position or heritable variation. Further results, in which beta-gal activity is correlated with NF-AT-binding activity, indicate that the concentration of NF-AT must exceed a critical threshold before transcription initiates. This threshold likely reflects the NF-AT concentration-dependent assembly of transcription complexes at the promoter. Similar constructs controlled by NF-kappa B or the entire interleukin-2 enhancer show bimodal expression patterns during induction, suggesting that thresholds set by the concentration of transcription factors may be a common property of inducible genes."}
GENIAcorpus
{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":23,"end":43},"obj":"protein_family_or_group"},{"id":"T2","span":{"begin":67,"end":80},"obj":"other_name"},{"id":"T3","span":{"begin":121,"end":144},"obj":"protein_family_or_group"},{"id":"T4","span":{"begin":161,"end":174},"obj":"cell_type"},{"id":"T5","span":{"begin":189,"end":205},"obj":"protein_family_or_group"},{"id":"T6","span":{"begin":241,"end":262},"obj":"protein_family_or_group"},{"id":"T7","span":{"begin":274,"end":279},"obj":"protein_molecule"},{"id":"T8","span":{"begin":284,"end":294},"obj":"protein_molecule"},{"id":"T9","span":{"begin":412,"end":432},"obj":"protein_family_or_group"},{"id":"T10","span":{"begin":436,"end":459},"obj":"cell_type"},{"id":"T11","span":{"begin":549,"end":567},"obj":"protein_molecule"},{"id":"T12","span":{"begin":569,"end":577},"obj":"protein_molecule"},{"id":"T13","span":{"begin":617,"end":622},"obj":"protein_molecule"},{"id":"T14","span":{"begin":654,"end":661},"obj":"cell_type"},{"id":"T15","span":{"begin":741,"end":746},"obj":"protein_molecule"},{"id":"T16","span":{"begin":768,"end":781},"obj":"other_name"},{"id":"T17","span":{"begin":789,"end":798},"obj":"DNA_domain_or_region"},{"id":"T18","span":{"begin":829,"end":861},"obj":"cell_line"},{"id":"T19","span":{"begin":862,"end":869},"obj":"cell_type"},{"id":"T20","span":{"begin":951,"end":959},"obj":"protein_molecule"},{"id":"T21","span":{"begin":1113,"end":1121},"obj":"protein_molecule"},{"id":"T22","span":{"begin":1150,"end":1155},"obj":"protein_molecule"},{"id":"T23","span":{"begin":1209,"end":1214},"obj":"protein_molecule"},{"id":"T24","span":{"begin":1255,"end":1268},"obj":"other_name"},{"id":"T25","span":{"begin":1315,"end":1320},"obj":"protein_molecule"},{"id":"T26","span":{"begin":1357,"end":1380},"obj":"protein_complex"},{"id":"T27","span":{"begin":1388,"end":1396},"obj":"DNA_domain_or_region"},{"id":"T28","span":{"begin":1431,"end":1441},"obj":"protein_molecule"},{"id":"T29","span":{"begin":1456,"end":1478},"obj":"DNA_domain_or_region"},{"id":"T30","span":{"begin":1484,"end":1511},"obj":"other_name"},{"id":"T31","span":{"begin":1585,"end":1606},"obj":"protein_family_or_group"},{"id":"T32","span":{"begin":1635,"end":1650},"obj":"DNA_family_or_group"}],"text":"Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.\nStimulation of T lymphocytes through their antigen receptor leads to the appearance of several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate the activity of a single transcription factor in individual viable cells, we have applied an assay that uses the fluorescence-activated cell sorter to quantitate beta-galactosidase (beta-gal). We have analyzed the distribution of NF-AT transcriptional activity among T cells undergoing activation by using a construct in which three tandem copies of the NF-AT-binding site directs transcription of the lacZ gene. Unexpectedly, stimulation of cloned stably transfected Jurkat T cells leads to a bimodal pattern of beta-gal expression in which some cells express no beta-gal and others express high levels. This expression pattern cannot be accounted for by cell-cycle position or heritable variation. Further results, in which beta-gal activity is correlated with NF-AT-binding activity, indicate that the concentration of NF-AT must exceed a critical threshold before transcription initiates. This threshold likely reflects the NF-AT concentration-dependent assembly of transcription complexes at the promoter. Similar constructs controlled by NF-kappa B or the entire interleukin-2 enhancer show bimodal expression patterns during induction, suggesting that thresholds set by the concentration of transcription factors may be a common property of inducible genes."}