PubMed:2050125 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2050125","sourcedb":"PubMed","sourceid":"2050125","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2050125","text":"Human erythroid 5-aminolevulinate synthase: promoter analysis and identification of an iron-responsive element in the mRNA.\n5-Aminolevulinate synthase (ALAS) catalyzes the first step of the heme biosynthetic pathway. cDNA clones for the human erythroid ALAS isozyme were isolated from a fetal liver library. It can be deduced that the erythroid ALAS precursor protein has a molecular weight of 64.6 kd, and is similar in size to the previously isolated human housekeeping ALAS precursor of molecular weight 70.6 kd. The mature mitochondrial forms of the erythroid and housekeeping ALAS isozymes are predicted to have molecular weights of 59.5 kd and 64.6 kd, respectively. The two isozymes show little amino acid identity in their N-terminal signal sequences but have considerable sequence identity in the C-terminal two-thirds of their proteins. An analysis of the immediate promoter of the human erythroid ALAS gene revealed several putative erythroid-specific cis-acting elements including both a GATA-1 and an NF-E2 binding site. An iron-responsive element (IRE) motif has been identified in the 5'-untranslated region of the human erythroid ALAS mRNA, but is not present in the housekeeping ALAS mRNA. Gel retardation experiments established that this IRE motif formed a protein - RNA complex with cytosolic extracts from human K562 cells and this binding was strongly competed with IRE transcripts from ferritin or transferrin receptor mRNAs. A transcript of the ALAS IRE, mutated in the conserved loop of the IRE, did not readily form this protein - RNA complex. These results suggest that the IRE motif in the ALAS mRNA is functional and imply that translation of the mRNA is controlled by cellular iron availability during erythropoiesis.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C2","span":{"begin":16,"end":42},"obj":"NP"},{"id":"C1","span":{"begin":0,"end":42},"obj":"NP"},{"id":"C3","span":{"begin":124,"end":157},"obj":"NP"},{"id":"C5","span":{"begin":253,"end":257},"obj":"NP"},{"id":"C4","span":{"begin":233,"end":265},"obj":"NP"},{"id":"C6","span":{"begin":345,"end":349},"obj":"NP"},{"id":"C8","span":{"begin":472,"end":476},"obj":"NP"},{"id":"C7","span":{"begin":453,"end":476},"obj":"NP"},{"id":"C10","span":{"begin":581,"end":585},"obj":"NP"},{"id":"C9","span":{"begin":550,"end":594},"obj":"NP"},{"id":"C11","span":{"begin":673,"end":689},"obj":"NP"},{"id":"C12","span":{"begin":831,"end":836},"obj":"NP"},{"id":"C14","span":{"begin":908,"end":912},"obj":"NP"},{"id":"C13","span":{"begin":888,"end":917},"obj":"NP"},{"id":"C15","span":{"begin":1034,"end":1072},"obj":"NP"},{"id":"C17","span":{"begin":1146,"end":1150},"obj":"NP"},{"id":"C16","span":{"begin":1126,"end":1155},"obj":"NP"},{"id":"C19","span":{"begin":1196,"end":1200},"obj":"NP"},{"id":"C18","span":{"begin":1179,"end":1205},"obj":"NP"},{"id":"C20","span":{"begin":1252,"end":1266},"obj":"NP"},{"id":"C21","span":{"begin":1388,"end":1403},"obj":"NP"},{"id":"C22","span":{"begin":1469,"end":1473},"obj":"NP"},{"id":"C23","span":{"begin":1597,"end":1610},"obj":"NP"},{"id":"C25","span":{"begin":1618,"end":1622},"obj":"NP"},{"id":"C24","span":{"begin":1614,"end":1627},"obj":"NP"},{"id":"C26","span":{"begin":1672,"end":1680},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C5","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C6","obj":"C5"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C6"},{"id":"R5","pred":"coref-ident","subj":"C10","obj":"C8"},{"id":"R6","pred":"coref-ident","subj":"C11","obj":"C9"},{"id":"R7","pred":"coref-pron","subj":"C12","obj":"C11"},{"id":"R8","pred":"coref-ident","subj":"C14","obj":"C10"},{"id":"R9","pred":"coref-ident","subj":"C13","obj":"C4"},{"id":"R10","pred":"coref-ident","subj":"C17","obj":"C14"},{"id":"R11","pred":"coref-other","subj":"C16","obj":"C13"},{"id":"R12","pred":"coref-ident","subj":"C19","obj":"C17"},{"id":"R13","pred":"coref-other","subj":"C18","obj":"C7"},{"id":"R14","pred":"coref-ident","subj":"C20","obj":"C15"},{"id":"R15","pred":"coref-other","subj":"C21","obj":"C20"},{"id":"R16","pred":"coref-ident","subj":"C22","obj":"C19"},{"id":"R17","pred":"coref-ident","subj":"C23","obj":"C20"},{"id":"R18","pred":"coref-ident","subj":"C25","obj":"C22"},{"id":"R19","pred":"coref-other","subj":"C24","obj":"C16"},{"id":"R20","pred":"coref-ident","subj":"C26","obj":"C24"}],"attributes":[{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C18","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C25","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C26","pred":"source","obj":"genia-medco-coref"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":527,"end":540},"obj":"HP_0001427"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":42},"obj":"protein"},{"id":"T2","span":{"begin":44,"end":52},"obj":"DNA"},{"id":"T3","span":{"begin":87,"end":110},"obj":"DNA"},{"id":"T4","span":{"begin":118,"end":122},"obj":"RNA"},{"id":"T5","span":{"begin":124,"end":150},"obj":"protein"},{"id":"T6","span":{"begin":152,"end":156},"obj":"protein"},{"id":"T7","span":{"begin":190,"end":194},"obj":"protein"},{"id":"T8","span":{"begin":217,"end":228},"obj":"DNA"},{"id":"T9","span":{"begin":237,"end":265},"obj":"protein"},{"id":"T10","span":{"begin":335,"end":367},"obj":"protein"},{"id":"T11","span":{"begin":453,"end":486},"obj":"protein"},{"id":"T12","span":{"begin":568,"end":594},"obj":"protein"},{"id":"T13","span":{"begin":681,"end":689},"obj":"protein"},{"id":"T14","span":{"begin":731,"end":758},"obj":"protein"},{"id":"T15","span":{"begin":806,"end":827},"obj":"protein"},{"id":"T16","span":{"begin":866,"end":884},"obj":"DNA"},{"id":"T17","span":{"begin":892,"end":917},"obj":"DNA"},{"id":"T18","span":{"begin":944,"end":982},"obj":"DNA"},{"id":"T19","span":{"begin":1000,"end":1006},"obj":"DNA"},{"id":"T20","span":{"begin":1014,"end":1032},"obj":"DNA"},{"id":"T21","span":{"begin":1037,"end":1072},"obj":"RNA"},{"id":"T22","span":{"begin":1130,"end":1155},"obj":"RNA"},{"id":"T23","span":{"begin":1183,"end":1205},"obj":"RNA"},{"id":"T24","span":{"begin":1257,"end":1266},"obj":"RNA"},{"id":"T25","span":{"begin":1276,"end":1297},"obj":"protein"},{"id":"T26","span":{"begin":1327,"end":1343},"obj":"cell_line"},{"id":"T27","span":{"begin":1388,"end":1403},"obj":"RNA"},{"id":"T28","span":{"begin":1409,"end":1447},"obj":"RNA"},{"id":"T29","span":{"begin":1469,"end":1477},"obj":"DNA"},{"id":"T30","span":{"begin":1516,"end":1519},"obj":"DNA"},{"id":"T31","span":{"begin":1547,"end":1568},"obj":"protein"},{"id":"T32","span":{"begin":1601,"end":1610},"obj":"RNA"},{"id":"T33","span":{"begin":1618,"end":1627},"obj":"RNA"},{"id":"T34","span":{"begin":1676,"end":1680},"obj":"RNA"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"},{"subj":"T32","pred":"source","obj":"jnlpba-st-training"},{"subj":"T33","pred":"source","obj":"jnlpba-st-training"},{"subj":"T34","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":123},"obj":"Sentence"},{"id":"S2","span":{"begin":124,"end":216},"obj":"Sentence"},{"id":"S3","span":{"begin":217,"end":307},"obj":"Sentence"},{"id":"S4","span":{"begin":308,"end":515},"obj":"Sentence"},{"id":"S5","span":{"begin":516,"end":672},"obj":"Sentence"},{"id":"S6","span":{"begin":673,"end":846},"obj":"Sentence"},{"id":"S7","span":{"begin":847,"end":1033},"obj":"Sentence"},{"id":"S8","span":{"begin":1034,"end":1206},"obj":"Sentence"},{"id":"S9","span":{"begin":1207,"end":1448},"obj":"Sentence"},{"id":"S10","span":{"begin":1449,"end":1569},"obj":"Sentence"},{"id":"S11","span":{"begin":1570,"end":1747},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S10","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S11","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":42},"obj":"protein_molecule"},{"id":"T2","span":{"begin":44,"end":52},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":87,"end":110},"obj":"DNA_domain_or_region"},{"id":"T4","span":{"begin":118,"end":122},"obj":"RNA_domain_or_region"},{"id":"T5","span":{"begin":124,"end":150},"obj":"protein_molecule"},{"id":"T6","span":{"begin":152,"end":156},"obj":"protein_molecule"},{"id":"T7","span":{"begin":190,"end":194},"obj":"protein_molecule"},{"id":"T8","span":{"begin":217,"end":228},"obj":"DNA_domain_or_region"},{"id":"T9","span":{"begin":237,"end":265},"obj":"protein_family_or_group"},{"id":"T10","span":{"begin":287,"end":298},"obj":"tissue"},{"id":"T11","span":{"begin":335,"end":367},"obj":"protein_molecule"},{"id":"T12","span":{"begin":453,"end":486},"obj":"protein_molecule"},{"id":"T13","span":{"begin":520,"end":546},"obj":"other_name"},{"id":"T14","span":{"begin":568,"end":594},"obj":"protein_family_or_group"},{"id":"T15","span":{"begin":617,"end":634},"obj":"other_name"},{"id":"T16","span":{"begin":681,"end":689},"obj":"protein_family_or_group"},{"id":"T17","span":{"begin":702,"end":721},"obj":"other_name"},{"id":"T18","span":{"begin":731,"end":758},"obj":"protein_domain_or_region"},{"id":"T19","span":{"begin":806,"end":827},"obj":"protein_domain_or_region"},{"id":"T20","span":{"begin":866,"end":884},"obj":"DNA_domain_or_region"},{"id":"T21","span":{"begin":892,"end":917},"obj":"DNA_domain_or_region"},{"id":"T22","span":{"begin":944,"end":982},"obj":"DNA_domain_or_region"},{"id":"T23","span":{"begin":1000,"end":1006},"obj":"DNA_domain_or_region"},{"id":"T24","span":{"begin":1014,"end":1032},"obj":"DNA_domain_or_region"},{"id":"T25","span":{"begin":1037,"end":1060},"obj":"DNA_domain_or_region"},{"id":"T26","span":{"begin":1100,"end":1122},"obj":"other_name"},{"id":"T27","span":{"begin":1130,"end":1155},"obj":"RNA_molecule"},{"id":"T28","span":{"begin":1183,"end":1205},"obj":"RNA_family_or_group"},{"id":"T29","span":{"begin":1257,"end":1266},"obj":"RNA_domain_or_region"},{"id":"T30","span":{"begin":1276,"end":1297},"obj":"protein_subunit"},{"id":"T31","span":{"begin":1303,"end":1321},"obj":"cell_component"},{"id":"T32","span":{"begin":1327,"end":1343},"obj":"cell_line"},{"id":"T33","span":{"begin":1388,"end":1403},"obj":"RNA_family_or_group"},{"id":"T34","span":{"begin":1469,"end":1477},"obj":"DNA_domain_or_region"},{"id":"T35","span":{"begin":1516,"end":1519},"obj":"DNA_domain_or_region"},{"id":"T36","span":{"begin":1547,"end":1568},"obj":"protein_complex"},{"id":"T37","span":{"begin":1601,"end":1610},"obj":"RNA_domain_or_region"},{"id":"T38","span":{"begin":1618,"end":1627},"obj":"RNA_domain_or_region"},{"id":"T39","span":{"begin":1676,"end":1680},"obj":"RNA_domain_or_region"},{"id":"T40","span":{"begin":1698,"end":1724},"obj":"other_name"},{"id":"T41","span":{"begin":1732,"end":1746},"obj":"other_name"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"},{"subj":"T41","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#ecc993","default":true},{"id":"PubmedHPO","color":"#af93ec"},{"id":"jnlpba-st-training","color":"#93ec95"},{"id":"pubmed-sentences-benchmark","color":"#ec93aa"},{"id":"GENIAcorpus","color":"#93c4ec"}]}]}}