PubMed:2010090 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2010090","sourcedb":"PubMed","sourceid":"2010090","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2010090","text":"A thymus-specific member of the HMG protein family regulates the human T cell receptor C alpha enhancer.\nThe human T cell-specific transcription factor TCF-1 alpha plays a key role in the tissue-specific activation of the T cell receptor (TCR) C alpha enhancer and binds to pyrimidine-rich elements (5'-PyCTTTG-3') present in a variety of other T cell-specific control regions. Using amino acid sequence information derived from the DNA affinity-purified protein, we have now isolated cDNA clones encoding TCF-1 alpha. The TCF-1 alpha cDNA contains a single 68-amino-acid domain that is homologous to a region conserved among high-mobility group (HMG) and nonhistone chromosomal proteins. Expression of full-length and mutant cDNA clones in bacteria reveal that the single HMG motif, which is predicted to contain two extended alpha-helical segments, is sufficient to direct the sequence-specific binding of TCF-1 alpha to DNA. Northern blot experiments demonstrate further that TCF-1 alpha mRNA is highly tissue specific, found primarily in the thymus or T cell lines. The immature CEM T cell line expresses relatively low levels of TCF-1 alpha mRNA, which are increased upon activation of these cells by phorbol esters. Interestingly, the cloned TCF-1 alpha protein is a potent transcriptional activator of the human TCR alpha enhancer in nonlymphoid cell lines, whereas the activity of the endogenous protein in T cell lines is strongly dependent on an additional T cell-specific protein that interacts with the core enhancer. TCF-1 alpha is currently unique among the newly emerging family of DNA-binding regulatory proteins that share the HMG motif in that it is a highly tissue-specific RNA polymerase II transcription factor.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":32,"end":35},"obj":"NP"},{"id":"C3","span":{"begin":61,"end":86},"obj":"NP"},{"id":"C2","span":{"begin":61,"end":103},"obj":"NP"},{"id":"C4","span":{"begin":105,"end":151},"obj":"NP"},{"id":"C5","span":{"begin":152,"end":163},"obj":"NP"},{"id":"C7","span":{"begin":218,"end":243},"obj":"NP"},{"id":"C6","span":{"begin":218,"end":260},"obj":"NP"},{"id":"C9","span":{"begin":506,"end":517},"obj":"NP"},{"id":"C8","span":{"begin":485,"end":517},"obj":"NP"},{"id":"C10","span":{"begin":519,"end":539},"obj":"NP"},{"id":"C11","span":{"begin":626,"end":651},"obj":"NP"},{"id":"C13","span":{"begin":773,"end":776},"obj":"NP"},{"id":"C12","span":{"begin":762,"end":782},"obj":"NP"},{"id":"C14","span":{"begin":784,"end":789},"obj":"NP"},{"id":"C15","span":{"begin":908,"end":919},"obj":"NP"},{"id":"C17","span":{"begin":979,"end":990},"obj":"NP"},{"id":"C16","span":{"begin":979,"end":995},"obj":"NP"},{"id":"C18","span":{"begin":1070,"end":1098},"obj":"NP"},{"id":"C20","span":{"begin":1134,"end":1145},"obj":"NP"},{"id":"C19","span":{"begin":1134,"end":1150},"obj":"NP"},{"id":"C21","span":{"begin":1152,"end":1157},"obj":"NP"},{"id":"C22","span":{"begin":1191,"end":1202},"obj":"NP"},{"id":"C23","span":{"begin":1248,"end":1259},"obj":"NP"},{"id":"C24","span":{"begin":1309,"end":1337},"obj":"NP"},{"id":"C25","span":{"begin":1530,"end":1541},"obj":"NP"},{"id":"C26","span":{"begin":1644,"end":1647},"obj":"NP"},{"id":"C28","span":{"begin":1640,"end":1653},"obj":"NP"},{"id":"C27","span":{"begin":1662,"end":1664},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-appos","subj":"C5","obj":"C4"},{"id":"R2","pred":"coref-ident","subj":"C7","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C6","obj":"C2"},{"id":"R4","pred":"coref-ident","subj":"C9","obj":"C5"},{"id":"R5","pred":"coref-other","subj":"C10","obj":"C8"},{"id":"R6","pred":"coref-ident","subj":"C11","obj":"C1"},{"id":"R7","pred":"coref-ident","subj":"C13","obj":"C11"},{"id":"R8","pred":"coref-relat","subj":"C14","obj":"C12"},{"id":"R9","pred":"coref-ident","subj":"C15","obj":"C9"},{"id":"R10","pred":"coref-ident","subj":"C17","obj":"C15"},{"id":"R11","pred":"coref-ident","subj":"C20","obj":"C17"},{"id":"R12","pred":"coref-ident","subj":"C19","obj":"C16"},{"id":"R13","pred":"coref-relat","subj":"C21","obj":"C19"},{"id":"R14","pred":"coref-ident","subj":"C22","obj":"C18"},{"id":"R15","pred":"coref-ident","subj":"C23","obj":"C20"},{"id":"R16","pred":"coref-ident","subj":"C24","obj":"C6"},{"id":"R17","pred":"coref-ident","subj":"C25","obj":"C23"},{"id":"R18","pred":"coref-ident","subj":"C26","obj":"C13"},{"id":"R19","pred":"coref-other","subj":"C28","obj":"C12"},{"id":"R20","pred":"coref-pron","subj":"C27","obj":"C25"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C9","pred":"source","obj":"genia-medco-coref"},{"subj":"C8","pred":"source","obj":"genia-medco-coref"},{"subj":"C10","pred":"source","obj":"genia-medco-coref"},{"subj":"C11","pred":"source","obj":"genia-medco-coref"},{"subj":"C13","pred":"source","obj":"genia-medco-coref"},{"subj":"C12","pred":"source","obj":"genia-medco-coref"},{"subj":"C14","pred":"source","obj":"genia-medco-coref"},{"subj":"C15","pred":"source","obj":"genia-medco-coref"},{"subj":"C17","pred":"source","obj":"genia-medco-coref"},{"subj":"C16","pred":"source","obj":"genia-medco-coref"},{"subj":"C18","pred":"source","obj":"genia-medco-coref"},{"subj":"C20","pred":"source","obj":"genia-medco-coref"},{"subj":"C19","pred":"source","obj":"genia-medco-coref"},{"subj":"C21","pred":"source","obj":"genia-medco-coref"},{"subj":"C22","pred":"source","obj":"genia-medco-coref"},{"subj":"C23","pred":"source","obj":"genia-medco-coref"},{"subj":"C24","pred":"source","obj":"genia-medco-coref"},{"subj":"C25","pred":"source","obj":"genia-medco-coref"},{"subj":"C26","pred":"source","obj":"genia-medco-coref"},{"subj":"C28","pred":"source","obj":"genia-medco-coref"},{"subj":"C27","pred":"source","obj":"genia-medco-coref"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":2,"end":24},"obj":"protein"},{"id":"T2","span":{"begin":32,"end":50},"obj":"protein"},{"id":"T3","span":{"begin":65,"end":103},"obj":"DNA"},{"id":"T4","span":{"begin":109,"end":151},"obj":"protein"},{"id":"T5","span":{"begin":152,"end":163},"obj":"protein"},{"id":"T6","span":{"begin":222,"end":237},"obj":"protein"},{"id":"T7","span":{"begin":239,"end":242},"obj":"protein"},{"id":"T8","span":{"begin":274,"end":298},"obj":"DNA"},{"id":"T9","span":{"begin":345,"end":376},"obj":"DNA"},{"id":"T10","span":{"begin":433,"end":462},"obj":"protein"},{"id":"T11","span":{"begin":485,"end":496},"obj":"DNA"},{"id":"T12","span":{"begin":506,"end":517},"obj":"protein"},{"id":"T13","span":{"begin":523,"end":539},"obj":"DNA"},{"id":"T14","span":{"begin":558,"end":578},"obj":"protein"},{"id":"T15","span":{"begin":626,"end":645},"obj":"protein"},{"id":"T16","span":{"begin":647,"end":650},"obj":"protein"},{"id":"T17","span":{"begin":656,"end":687},"obj":"protein"},{"id":"T18","span":{"begin":703,"end":714},"obj":"DNA"},{"id":"T19","span":{"begin":719,"end":737},"obj":"DNA"},{"id":"T20","span":{"begin":766,"end":782},"obj":"protein"},{"id":"T21","span":{"begin":827,"end":849},"obj":"protein"},{"id":"T22","span":{"begin":908,"end":919},"obj":"protein"},{"id":"T23","span":{"begin":979,"end":995},"obj":"RNA"},{"id":"T24","span":{"begin":1046,"end":1068},"obj":"cell_line"},{"id":"T25","span":{"begin":1074,"end":1098},"obj":"cell_line"},{"id":"T26","span":{"begin":1134,"end":1150},"obj":"RNA"},{"id":"T27","span":{"begin":1248,"end":1267},"obj":"protein"},{"id":"T28","span":{"begin":1280,"end":1305},"obj":"protein"},{"id":"T29","span":{"begin":1313,"end":1337},"obj":"DNA"},{"id":"T30","span":{"begin":1341,"end":1363},"obj":"cell_line"},{"id":"T31","span":{"begin":1393,"end":1411},"obj":"protein"},{"id":"T32","span":{"begin":1415,"end":1427},"obj":"cell_line"},{"id":"T33","span":{"begin":1467,"end":1490},"obj":"cell_line"},{"id":"T34","span":{"begin":1530,"end":1541},"obj":"protein"},{"id":"T35","span":{"begin":1597,"end":1628},"obj":"protein"},{"id":"T36","span":{"begin":1644,"end":1653},"obj":"protein"},{"id":"T37","span":{"begin":1670,"end":1710},"obj":"protein"},{"id":"T38","span":{"begin":1711,"end":1731},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"},{"subj":"T22","pred":"source","obj":"jnlpba-st-training"},{"subj":"T23","pred":"source","obj":"jnlpba-st-training"},{"subj":"T24","pred":"source","obj":"jnlpba-st-training"},{"subj":"T25","pred":"source","obj":"jnlpba-st-training"},{"subj":"T26","pred":"source","obj":"jnlpba-st-training"},{"subj":"T27","pred":"source","obj":"jnlpba-st-training"},{"subj":"T28","pred":"source","obj":"jnlpba-st-training"},{"subj":"T29","pred":"source","obj":"jnlpba-st-training"},{"subj":"T30","pred":"source","obj":"jnlpba-st-training"},{"subj":"T31","pred":"source","obj":"jnlpba-st-training"},{"subj":"T32","pred":"source","obj":"jnlpba-st-training"},{"subj":"T33","pred":"source","obj":"jnlpba-st-training"},{"subj":"T34","pred":"source","obj":"jnlpba-st-training"},{"subj":"T35","pred":"source","obj":"jnlpba-st-training"},{"subj":"T36","pred":"source","obj":"jnlpba-st-training"},{"subj":"T37","pred":"source","obj":"jnlpba-st-training"},{"subj":"T38","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":104},"obj":"Sentence"},{"id":"S2","span":{"begin":105,"end":377},"obj":"Sentence"},{"id":"S3","span":{"begin":378,"end":518},"obj":"Sentence"},{"id":"S4","span":{"begin":519,"end":688},"obj":"Sentence"},{"id":"S5","span":{"begin":689,"end":927},"obj":"Sentence"},{"id":"S6","span":{"begin":928,"end":1069},"obj":"Sentence"},{"id":"S7","span":{"begin":1070,"end":1221},"obj":"Sentence"},{"id":"S8","span":{"begin":1222,"end":1529},"obj":"Sentence"},{"id":"S9","span":{"begin":1530,"end":1732},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":2,"end":24},"obj":"protein_family_or_group"},{"id":"T2","span":{"begin":32,"end":50},"obj":"protein_family_or_group"},{"id":"T3","span":{"begin":65,"end":94},"obj":"protein_subunit"},{"id":"T4","span":{"begin":109,"end":130},"obj":"protein_family_or_group"},{"id":"T5","span":{"begin":131,"end":151},"obj":"protein_family_or_group"},{"id":"T6","span":{"begin":152,"end":163},"obj":"protein_molecule"},{"id":"T7","span":{"begin":222,"end":237},"obj":"protein_family_or_group"},{"id":"T8","span":{"begin":239,"end":242},"obj":"protein_family_or_group"},{"id":"T9","span":{"begin":274,"end":284},"obj":"nucleotide"},{"id":"T10","span":{"begin":300,"end":313},"obj":"polynucleotide"},{"id":"T11","span":{"begin":345,"end":376},"obj":"DNA_domain_or_region"},{"id":"T12","span":{"begin":384,"end":403},"obj":"protein_N/A"},{"id":"T13","span":{"begin":433,"end":462},"obj":"protein_molecule"},{"id":"T14","span":{"begin":485,"end":496},"obj":"DNA_family_or_group"},{"id":"T15","span":{"begin":506,"end":517},"obj":"protein_molecule"},{"id":"T16","span":{"begin":523,"end":539},"obj":"DNA_family_or_group"},{"id":"T17","span":{"begin":558,"end":578},"obj":"protein_domain_or_region"},{"id":"T18","span":{"begin":626,"end":645},"obj":"protein_family_or_group"},{"id":"T19","span":{"begin":647,"end":650},"obj":"protein_family_or_group"},{"id":"T20","span":{"begin":656,"end":687},"obj":"protein_family_or_group"},{"id":"T21","span":{"begin":703,"end":714},"obj":"DNA_family_or_group"},{"id":"T22","span":{"begin":719,"end":737},"obj":"DNA_family_or_group"},{"id":"T23","span":{"begin":827,"end":849},"obj":"protein_domain_or_region"},{"id":"T24","span":{"begin":908,"end":919},"obj":"protein_molecule"},{"id":"T25","span":{"begin":928,"end":953},"obj":"other_name"},{"id":"T26","span":{"begin":979,"end":990},"obj":"protein_molecule"},{"id":"T27","span":{"begin":1074,"end":1098},"obj":"cell_line"},{"id":"T28","span":{"begin":1134,"end":1145},"obj":"protein_molecule"},{"id":"T29","span":{"begin":1206,"end":1220},"obj":"other_organic_compound"},{"id":"T30","span":{"begin":1248,"end":1259},"obj":"protein_molecule"},{"id":"T31","span":{"begin":1280,"end":1305},"obj":"protein_family_or_group"},{"id":"T32","span":{"begin":1313,"end":1337},"obj":"DNA_domain_or_region"},{"id":"T33","span":{"begin":1341,"end":1363},"obj":"cell_line"},{"id":"T34","span":{"begin":1393,"end":1411},"obj":"protein_family_or_group"},{"id":"T35","span":{"begin":1415,"end":1427},"obj":"cell_line"},{"id":"T36","span":{"begin":1467,"end":1490},"obj":"cell_line"},{"id":"T37","span":{"begin":1530,"end":1541},"obj":"protein_molecule"},{"id":"T38","span":{"begin":1597,"end":1628},"obj":"protein_family_or_group"},{"id":"T39","span":{"begin":1670,"end":1710},"obj":"protein_molecule"},{"id":"T40","span":{"begin":1711,"end":1731},"obj":"protein_family_or_group"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"},{"subj":"T29","pred":"source","obj":"GENIAcorpus"},{"subj":"T30","pred":"source","obj":"GENIAcorpus"},{"subj":"T31","pred":"source","obj":"GENIAcorpus"},{"subj":"T32","pred":"source","obj":"GENIAcorpus"},{"subj":"T33","pred":"source","obj":"GENIAcorpus"},{"subj":"T34","pred":"source","obj":"GENIAcorpus"},{"subj":"T35","pred":"source","obj":"GENIAcorpus"},{"subj":"T36","pred":"source","obj":"GENIAcorpus"},{"subj":"T37","pred":"source","obj":"GENIAcorpus"},{"subj":"T38","pred":"source","obj":"GENIAcorpus"},{"subj":"T39","pred":"source","obj":"GENIAcorpus"},{"subj":"T40","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#deec93","default":true},{"id":"jnlpba-st-training","color":"#e093ec"},{"id":"pubmed-sentences-benchmark","color":"#93ecc6"},{"id":"GENIAcorpus","color":"#ecac93"}]}]}}