PubMed:20073421 JSONTXT

{"project":"bionlp-st-bb3-2016-training","denotations":[{"id":"T1","span":{"begin":0,"end":139},"obj":"Title"},{"id":"T2","span":{"begin":152,"end":275},"obj":"Paragraph"},{"id":"T3","span":{"begin":282,"end":458},"obj":"Paragraph"},{"id":"T4","span":{"begin":468,"end":739},"obj":"Paragraph"},{"id":"T5","span":{"begin":753,"end":893},"obj":"Paragraph"},{"id":"T6","span":{"begin":71,"end":97},"obj":"Bacteria"},{"id":"T7","span":{"begin":101,"end":107},"obj":"Habitat"},{"id":"T8","span":{"begin":101,"end":118},"obj":"Habitat"},{"id":"T9","span":{"begin":101,"end":138},"obj":"Habitat"},{"id":"T10","span":{"begin":152,"end":174},"obj":"Bacteria"},{"id":"T11","span":{"begin":197,"end":202},"obj":"Habitat"},{"id":"T12","span":{"begin":263,"end":274},"obj":"Habitat"},{"id":"T13","span":{"begin":263,"end":268},"obj":"Habitat"},{"id":"T14","span":{"begin":318,"end":335},"obj":"Bacteria"},{"id":"T15","span":{"begin":346,"end":356},"obj":"Habitat"},{"id":"T16","span":{"begin":346,"end":376},"obj":"Habitat"},{"id":"T17","span":{"begin":423,"end":430},"obj":"Bacteria"},{"id":"T18","span":{"begin":483,"end":493},"obj":"Habitat"},{"id":"T19","span":{"begin":596,"end":609},"obj":"Bacteria"},{"id":"T20","span":{"begin":715,"end":728},"obj":"Bacteria"},{"id":"T21","span":{"begin":768,"end":782},"obj":"Habitat"},{"id":"T22","span":{"begin":879,"end":892},"obj":"Bacteria"}],"text":"The effect of interferon-gamma and lipopolysaccharide on the growth of Francisella tularensis LVS in murine macrophage-like cell line J774.\nBACKGROUND: Francisella tularensis, a causative agent of human tularemia, displaying the ability to proliferate inside the human cells.\nAIMS: To evaluate the growth potential of F. tularensis LVS strain in macrophage-like cell line J774 modulated by recombinant interferon gamma and E. coli derived lipopolysaccharide.\nRESULTS: Stimulation of J774 cells either by interferon-gamma or lipopolysaccharide alone, or especially in combination before infection F. tularensis, revealed protective effects. Higher concentrations of stimulating agents were needed to inhibit ongoing F. tularensis infection.\nCONCLUSIONS: Stimulation of J774 cell line by combination of interferon-gamma with lipopolysaccharide inhibits the intracellular growth of F. tularensis."}