PubMed:20005218 JSONTXT

{"project":"LitCoin-sentences","denotations":[{"id":"T1","span":{"begin":0,"end":165},"obj":"Sentence"},{"id":"T2","span":{"begin":166,"end":177},"obj":"Sentence"},{"id":"T3","span":{"begin":178,"end":276},"obj":"Sentence"},{"id":"T4","span":{"begin":277,"end":434},"obj":"Sentence"},{"id":"T5","span":{"begin":435,"end":443},"obj":"Sentence"},{"id":"T6","span":{"begin":444,"end":608},"obj":"Sentence"},{"id":"T7","span":{"begin":609,"end":755},"obj":"Sentence"},{"id":"T8","span":{"begin":756,"end":764},"obj":"Sentence"},{"id":"T9","span":{"begin":765,"end":917},"obj":"Sentence"},{"id":"T10","span":{"begin":918,"end":1157},"obj":"Sentence"},{"id":"T11","span":{"begin":1158,"end":1372},"obj":"Sentence"},{"id":"T12","span":{"begin":1373,"end":1585},"obj":"Sentence"},{"id":"T13","span":{"begin":1586,"end":1598},"obj":"Sentence"},{"id":"T14","span":{"begin":1599,"end":1709},"obj":"Sentence"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-entities-OrganismTaxon-PD","denotations":[{"id":"T1","span":{"begin":178,"end":182},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":352,"end":357},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1630,"end":1635},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"NCBItxid:10095"},{"id":"A2","pred":"db_id","subj":"T1","obj":"NCBItxid:10088"},{"id":"A3","pred":"db_id","subj":"T3","obj":"NCBItxid:9606"},{"id":"A4","pred":"db_id","subj":"T4","obj":"NCBItxid:9606"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-entities","denotations":[{"id":"6859","span":{"begin":77,"end":83},"obj":"GeneOrGeneProduct"},{"id":"6860","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6861","span":{"begin":178,"end":182},"obj":"OrganismTaxon"},{"id":"6862","span":{"begin":203,"end":209},"obj":"GeneOrGeneProduct"},{"id":"6863","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6864","span":{"begin":302,"end":308},"obj":"SequenceVariant"},{"id":"6865","span":{"begin":352,"end":357},"obj":"OrganismTaxon"},{"id":"6866","span":{"begin":358,"end":364},"obj":"GeneOrGeneProduct"},{"id":"6867","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6868","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6869","span":{"begin":471,"end":477},"obj":"SequenceVariant"},{"id":"6870","span":{"begin":494,"end":500},"obj":"GeneOrGeneProduct"},{"id":"6871","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6872","span":{"begin":557,"end":565},"obj":"OrganismTaxon"},{"id":"6873","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6874","span":{"begin":586,"end":598},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6875","span":{"begin":634,"end":637},"obj":"SequenceVariant"},{"id":"6876","span":{"begin":658,"end":664},"obj":"SequenceVariant"},{"id":"6877","span":{"begin":814,"end":820},"obj":"SequenceVariant"},{"id":"6878","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6879","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6880","span":{"begin":1009,"end":1015},"obj":"SequenceVariant"},{"id":"6881","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6882","span":{"begin":1042,"end":1048},"obj":"SequenceVariant"},{"id":"6883","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6884","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"6885","span":{"begin":1242,"end":1247},"obj":"SequenceVariant"},{"id":"6886","span":{"begin":1426,"end":1431},"obj":"SequenceVariant"},{"id":"6887","span":{"begin":1481,"end":1487},"obj":"SequenceVariant"},{"id":"6888","span":{"begin":1510,"end":1516},"obj":"GeneOrGeneProduct"},{"id":"6889","span":{"begin":1571,"end":1576},"obj":"SequenceVariant"},{"id":"6890","span":{"begin":1603,"end":1609},"obj":"SequenceVariant"},{"id":"6891","span":{"begin":1630,"end":1635},"obj":"OrganismTaxon"},{"id":"6892","span":{"begin":1636,"end":1642},"obj":"GeneOrGeneProduct"},{"id":"6893","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"db_id","subj":"6859","obj":"NCBIGene:9365"},{"id":"A2","pred":"db_id","subj":"6860","obj":"MESH:D000075222"},{"id":"A3","pred":"db_id","subj":"6861","obj":"NCBITaxon:10090"},{"id":"A4","pred":"db_id","subj":"6862","obj":"NCBIGene:16591"},{"id":"A5","pred":"db_id","subj":"6863","obj":"MESH:D001161"},{"id":"A6","pred":"db_id","subj":"6864","obj":"DBSNP:rs1207568"},{"id":"A7","pred":"db_id","subj":"6865","obj":"NCBITaxon:9606"},{"id":"A8","pred":"db_id","subj":"6866","obj":"NCBIGene:9365"},{"id":"A9","pred":"db_id","subj":"6867","obj":"MESH:D000075222"},{"id":"A10","pred":"db_id","subj":"6868","obj":"MESH:D000075222"},{"id":"A11","pred":"db_id","subj":"6869","obj":"DBSNP:rs1207568"},{"id":"A12","pred":"db_id","subj":"6870","obj":"NCBIGene:9365"},{"id":"A13","pred":"db_id","subj":"6871","obj":"MESH:D000075222"},{"id":"A14","pred":"db_id","subj":"6872","obj":"NCBITaxon:9606"},{"id":"A15","pred":"db_id","subj":"6873","obj":"MESH:D000075222"},{"id":"A16","pred":"db_id","subj":"6874","obj":"MESH:D006973"},{"id":"A17","pred":"db_id","subj":"6875","obj":"DBSNP:rs1207568"},{"id":"A18","pred":"db_id","subj":"6876","obj":"DBSNP:rs1207568"},{"id":"A19","pred":"db_id","subj":"6877","obj":"DBSNP:rs1207568"},{"id":"A20","pred":"db_id","subj":"6878","obj":"MESH:D000075222"},{"id":"A21","pred":"db_id","subj":"6879","obj":"MESH:D006973"},{"id":"A22","pred":"db_id","subj":"6880","obj":"DBSNP:rs1207568"},{"id":"A23","pred":"db_id","subj":"6881","obj":"MESH:D000075222"},{"id":"A24","pred":"db_id","subj":"6882","obj":"DBSNP:rs1207568"},{"id":"A25","pred":"db_id","subj":"6883","obj":"MESH:D000075222"},{"id":"A26","pred":"db_id","subj":"6884","obj":"MESH:D000075222"},{"id":"A27","pred":"db_id","subj":"6885","obj":"DBSNP:rs1207568"},{"id":"A28","pred":"db_id","subj":"6886","obj":"DBSNP:rs1207568"},{"id":"A29","pred":"db_id","subj":"6887","obj":"DBSNP:rs1207568"},{"id":"A30","pred":"db_id","subj":"6888","obj":"NCBIGene:9365"},{"id":"A31","pred":"db_id","subj":"6889","obj":"DBSNP:rs1207568"},{"id":"A32","pred":"db_id","subj":"6890","obj":"DBSNP:rs1207568"},{"id":"A33","pred":"db_id","subj":"6891","obj":"NCBITaxon:9606"},{"id":"A34","pred":"db_id","subj":"6892","obj":"NCBIGene:9365"},{"id":"A35","pred":"db_id","subj":"6893","obj":"MESH:D000075222"}],"namespaces":[{"prefix":"_base","uri":"https://w3id.org/biolink/vocab/"},{"prefix":"MESH","uri":"http://id.nlm.nih.gov/mesh/"},{"prefix":"NCBITaxon","uri":"https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id="},{"prefix":"NCBIGene","uri":"https://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"OMIM","uri":"https://www.omim.org/entry/"},{"prefix":"DBSNP","uri":"https://www.ncbi.nlm.nih.gov/snp/"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin_Mondo","denotations":[{"id":"T1","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0001134"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0001134"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-SeqVar","denotations":[{"id":"T1","span":{"begin":302,"end":308},"obj":"SequenceVariant"},{"id":"T2","span":{"begin":471,"end":477},"obj":"SequenceVariant"},{"id":"T3","span":{"begin":658,"end":664},"obj":"SequenceVariant"},{"id":"T4","span":{"begin":814,"end":820},"obj":"SequenceVariant"},{"id":"T5","span":{"begin":1009,"end":1015},"obj":"SequenceVariant"},{"id":"T6","span":{"begin":1042,"end":1048},"obj":"SequenceVariant"},{"id":"T7","span":{"begin":1243,"end":1247},"obj":"SequenceVariant"},{"id":"T8","span":{"begin":1427,"end":1431},"obj":"SequenceVariant"},{"id":"T9","span":{"begin":1481,"end":1487},"obj":"SequenceVariant"},{"id":"T10","span":{"begin":1572,"end":1576},"obj":"SequenceVariant"},{"id":"T11","span":{"begin":1603,"end":1609},"obj":"SequenceVariant"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-GeneOrGeneProduct-v0","denotations":[{"id":"T1","span":{"begin":77,"end":83},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":122,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T3","span":{"begin":178,"end":182},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":188,"end":195},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":203,"end":209},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":223,"end":231},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":358,"end":364},"obj":"GeneOrGeneProduct"},{"id":"T8","span":{"begin":416,"end":428},"obj":"GeneOrGeneProduct"},{"id":"T9","span":{"begin":430,"end":432},"obj":"GeneOrGeneProduct"},{"id":"T10","span":{"begin":435,"end":442},"obj":"GeneOrGeneProduct"},{"id":"T11","span":{"begin":494,"end":500},"obj":"GeneOrGeneProduct"},{"id":"T12","span":{"begin":520,"end":522},"obj":"GeneOrGeneProduct"},{"id":"T13","span":{"begin":523,"end":527},"obj":"GeneOrGeneProduct"},{"id":"T14","span":{"begin":571,"end":573},"obj":"GeneOrGeneProduct"},{"id":"T15","span":{"begin":586,"end":598},"obj":"GeneOrGeneProduct"},{"id":"T16","span":{"begin":617,"end":623},"obj":"GeneOrGeneProduct"},{"id":"T17","span":{"begin":683,"end":696},"obj":"GeneOrGeneProduct"},{"id":"T18","span":{"begin":729,"end":739},"obj":"GeneOrGeneProduct"},{"id":"T19","span":{"begin":846,"end":848},"obj":"GeneOrGeneProduct"},{"id":"T20","span":{"begin":857,"end":869},"obj":"GeneOrGeneProduct"},{"id":"T21","span":{"begin":1034,"end":1036},"obj":"GeneOrGeneProduct"},{"id":"T22","span":{"begin":1095,"end":1097},"obj":"GeneOrGeneProduct"},{"id":"T23","span":{"begin":1160,"end":1168},"obj":"GeneOrGeneProduct"},{"id":"T24","span":{"begin":1232,"end":1234},"obj":"GeneOrGeneProduct"},{"id":"T25","span":{"begin":1255,"end":1263},"obj":"GeneOrGeneProduct"},{"id":"T26","span":{"begin":1364,"end":1371},"obj":"GeneOrGeneProduct"},{"id":"T27","span":{"begin":1382,"end":1392},"obj":"GeneOrGeneProduct"},{"id":"T28","span":{"begin":1432,"end":1439},"obj":"GeneOrGeneProduct"},{"id":"T29","span":{"begin":1510,"end":1516},"obj":"GeneOrGeneProduct"},{"id":"T30","span":{"begin":1542,"end":1552},"obj":"GeneOrGeneProduct"},{"id":"T31","span":{"begin":1553,"end":1561},"obj":"GeneOrGeneProduct"},{"id":"T32","span":{"begin":1577,"end":1584},"obj":"GeneOrGeneProduct"},{"id":"T33","span":{"begin":1636,"end":1642},"obj":"GeneOrGeneProduct"},{"id":"T34","span":{"begin":1667,"end":1669},"obj":"GeneOrGeneProduct"},{"id":"T35","span":{"begin":1674,"end":1682},"obj":"GeneOrGeneProduct"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-GeneOrGeneProduct-v2","denotations":[{"id":"T1","span":{"begin":77,"end":83},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":122,"end":134},"obj":"GeneOrGeneProduct"},{"id":"T3","span":{"begin":203,"end":209},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":223,"end":231},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":358,"end":364},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":416,"end":428},"obj":"GeneOrGeneProduct"},{"id":"T7","span":{"begin":494,"end":500},"obj":"GeneOrGeneProduct"},{"id":"T8","span":{"begin":683,"end":696},"obj":"GeneOrGeneProduct"},{"id":"T9","span":{"begin":729,"end":739},"obj":"GeneOrGeneProduct"},{"id":"T10","span":{"begin":857,"end":869},"obj":"GeneOrGeneProduct"},{"id":"T11","span":{"begin":1160,"end":1168},"obj":"GeneOrGeneProduct"},{"id":"T12","span":{"begin":1382,"end":1392},"obj":"GeneOrGeneProduct"},{"id":"T13","span":{"begin":1432,"end":1439},"obj":"GeneOrGeneProduct"},{"id":"T14","span":{"begin":1510,"end":1516},"obj":"GeneOrGeneProduct"},{"id":"T15","span":{"begin":1542,"end":1552},"obj":"GeneOrGeneProduct"},{"id":"T16","span":{"begin":1577,"end":1584},"obj":"GeneOrGeneProduct"},{"id":"T17","span":{"begin":1636,"end":1642},"obj":"GeneOrGeneProduct"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-Disease-MeSH","denotations":[{"id":"T1","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":586,"end":598},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T13","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A3","pred":"originalLabel","subj":"T3","obj":"D000075222"},{"id":"A5","pred":"originalLabel","subj":"T5","obj":"D000075222"},{"id":"A2","pred":"originalLabel","subj":"T2","obj":"D001161"},{"id":"A8","pred":"originalLabel","subj":"T8","obj":"D000075222"},{"id":"A9","pred":"originalLabel","subj":"T9","obj":"D006973"},{"id":"A13","pred":"originalLabel","subj":"T13","obj":"D000075222"},{"id":"A4","pred":"originalLabel","subj":"T4","obj":"D000075222"},{"id":"A1","pred":"originalLabel","subj":"T1","obj":"D000075222"},{"id":"A10","pred":"originalLabel","subj":"T10","obj":"D000075222"},{"id":"A12","pred":"originalLabel","subj":"T12","obj":"D000075222"},{"id":"A11","pred":"originalLabel","subj":"T11","obj":"D000075222"},{"id":"A6","pred":"originalLabel","subj":"T6","obj":"D000075222"},{"id":"A7","pred":"originalLabel","subj":"T7","obj":"D006973"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-GeneOrGeneProduct-v3","denotations":[{"id":"T1","span":{"begin":77,"end":83},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":203,"end":209},"obj":"GeneOrGeneProduct"},{"id":"T3","span":{"begin":358,"end":364},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":494,"end":500},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":1510,"end":1516},"obj":"GeneOrGeneProduct"},{"id":"T6","span":{"begin":1636,"end":1642},"obj":"GeneOrGeneProduct"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin_Mondo_095","denotations":[{"id":"T1","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0005044"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0002277"},{"id":"A12","pred":"mondo_id","subj":"T12","obj":"0001134"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0001134"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0001134"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"0001134"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0001134"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0001134"},{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0001134"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0001134"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"0001134"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0001134"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-MeSH-Disease-2","denotations":[{"id":"T1","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A4","pred":"ID:","subj":"T4","obj":"D000075222"},{"id":"A1","pred":"ID:","subj":"T1","obj":"D000075222"},{"id":"A9","pred":"ID:","subj":"T9","obj":"D000075222"},{"id":"A7","pred":"ID:","subj":"T7","obj":"D000075222"},{"id":"A3","pred":"ID:","subj":"T3","obj":"D000075222"},{"id":"A2","pred":"ID:","subj":"T2","obj":"D001161"},{"id":"A11","pred":"ID:","subj":"T11","obj":"D000075222"},{"id":"A8","pred":"ID:","subj":"T8","obj":"D006973"},{"id":"A6","pred":"ID:","subj":"T6","obj":"D000075222"},{"id":"A10","pred":"ID:","subj":"T10","obj":"D000075222"},{"id":"A12","pred":"ID:","subj":"T12","obj":"D000075222"},{"id":"A5","pred":"ID:","subj":"T5","obj":"D000075222"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-MONDO_bioort2019","denotations":[{"id":"T1","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A2","pred":"#label","subj":"T2","obj":"D001161"},{"id":"A6","pred":"#label","subj":"T6","obj":"D000075222"},{"id":"A8","pred":"#label","subj":"T8","obj":"D006973"},{"id":"A3","pred":"#label","subj":"T3","obj":"D000075222"},{"id":"A9","pred":"#label","subj":"T9","obj":"D000075222"},{"id":"A10","pred":"#label","subj":"T10","obj":"D000075222"},{"id":"A12","pred":"#label","subj":"T12","obj":"D000075222"},{"id":"A7","pred":"#label","subj":"T7","obj":"D000075222"},{"id":"A11","pred":"#label","subj":"T11","obj":"D000075222"},{"id":"A4","pred":"#label","subj":"T4","obj":"D000075222"},{"id":"A1","pred":"#label","subj":"T1","obj":"D000075222"},{"id":"A5","pred":"#label","subj":"T5","obj":"D000075222"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-NCBITaxon-2","denotations":[{"id":"T1","span":{"begin":178,"end":182},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":352,"end":357},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":557,"end":565},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1630,"end":1635},"obj":"OrganismTaxon"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"LitCoin-training-merged","denotations":[{"id":"T6","span":{"begin":1636,"end":1642},"obj":"GeneOrGeneProduct"},{"id":"T5","span":{"begin":1510,"end":1516},"obj":"GeneOrGeneProduct"},{"id":"T4","span":{"begin":494,"end":500},"obj":"GeneOrGeneProduct"},{"id":"T3","span":{"begin":358,"end":364},"obj":"GeneOrGeneProduct"},{"id":"T2","span":{"begin":203,"end":209},"obj":"GeneOrGeneProduct"},{"id":"T1","span":{"begin":77,"end":83},"obj":"GeneOrGeneProduct"},{"id":"T12","span":{"begin":1667,"end":1669},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1232,"end":1234},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1095,"end":1097},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1034,"end":1036},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":857,"end":869},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":846,"end":848},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T88188","span":{"begin":571,"end":573},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11793","span":{"begin":520,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T32541","span":{"begin":430,"end":432},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T36593","span":{"begin":406,"end":428},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T51792","span":{"begin":259,"end":275},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4158","span":{"begin":112,"end":134},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T24394","span":{"begin":1630,"end":1635},"obj":"OrganismTaxon"},{"id":"T87251","span":{"begin":557,"end":565},"obj":"OrganismTaxon"},{"id":"T93486","span":{"begin":352,"end":357},"obj":"OrganismTaxon"},{"id":"T74375","span":{"begin":178,"end":182},"obj":"OrganismTaxon"},{"id":"T21071","span":{"begin":1603,"end":1609},"obj":"SequenceVariant"},{"id":"T71508","span":{"begin":1572,"end":1576},"obj":"SequenceVariant"},{"id":"T8254","span":{"begin":1481,"end":1487},"obj":"SequenceVariant"},{"id":"T69851","span":{"begin":1427,"end":1431},"obj":"SequenceVariant"},{"id":"T64331","span":{"begin":1243,"end":1247},"obj":"SequenceVariant"},{"id":"T407","span":{"begin":1042,"end":1048},"obj":"SequenceVariant"},{"id":"T92142","span":{"begin":1009,"end":1015},"obj":"SequenceVariant"},{"id":"T61940","span":{"begin":814,"end":820},"obj":"SequenceVariant"},{"id":"T21169","span":{"begin":658,"end":664},"obj":"SequenceVariant"},{"id":"T15828","span":{"begin":471,"end":477},"obj":"SequenceVariant"},{"id":"T47737","span":{"begin":302,"end":308},"obj":"SequenceVariant"}],"attributes":[{"id":"A12","pred":"#label","subj":"T12","obj":"D000075222"},{"id":"A11","pred":"#label","subj":"T11","obj":"D000075222"},{"id":"A10","pred":"#label","subj":"T10","obj":"D000075222"},{"id":"A9","pred":"#label","subj":"T9","obj":"D000075222"},{"id":"A8","pred":"#label","subj":"T8","obj":"D006973"},{"id":"A7","pred":"#label","subj":"T7","obj":"D000075222"},{"id":"A6","pred":"#label","subj":"T88188","obj":"D000075222"},{"id":"A5","pred":"#label","subj":"T11793","obj":"D000075222"},{"id":"A4","pred":"#label","subj":"T32541","obj":"D000075222"},{"id":"A3","pred":"#label","subj":"T36593","obj":"D000075222"},{"id":"A2","pred":"#label","subj":"T51792","obj":"D001161"},{"id":"A1","pred":"#label","subj":"T4158","obj":"D000075222"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":203,"end":209},"obj":"gene:9365"},{"id":"T1","span":{"begin":259,"end":275},"obj":"disease:C0003850"},{"id":"T2","span":{"begin":358,"end":364},"obj":"gene:9365"},{"id":"T3","span":{"begin":406,"end":428},"obj":"disease:C0085580"},{"id":"T4","span":{"begin":358,"end":364},"obj":"gene:9365"},{"id":"T5","span":{"begin":430,"end":432},"obj":"disease:C0085580"},{"id":"T6","span":{"begin":494,"end":500},"obj":"gene:9365"},{"id":"T7","span":{"begin":520,"end":522},"obj":"disease:C0085580"},{"id":"T8","span":{"begin":494,"end":500},"obj":"gene:9365"},{"id":"T9","span":{"begin":571,"end":573},"obj":"disease:C0085580"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":259,"end":275},"obj":"HP_0002634"},{"id":"T2","span":{"begin":416,"end":428},"obj":"HP_0000822"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"Allie","denotations":[{"id":"SS1_20005218_3_0","span":{"begin":406,"end":428},"obj":"expanded"},{"id":"SS2_20005218_3_0","span":{"begin":430,"end":432},"obj":"abbr"}],"relations":[{"id":"AE1_20005218_3_0","pred":"abbreviatedTo","subj":"SS1_20005218_3_0","obj":"SS2_20005218_3_0"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"20005218-0#77#83#gene9365","span":{"begin":77,"end":83},"obj":"gene9365"},{"id":"20005218-0#112#134#diseaseC0085580","span":{"begin":112,"end":134},"obj":"diseaseC0085580"},{"id":"20005218-1#25#31#gene9365","span":{"begin":203,"end":209},"obj":"gene9365"},{"id":"20005218-1#81#97#diseaseC0003850","span":{"begin":259,"end":275},"obj":"diseaseC0003850"}],"relations":[{"id":"77#83#gene9365112#134#diseaseC0085580","pred":"associated_with","subj":"20005218-0#77#83#gene9365","obj":"20005218-0#112#134#diseaseC0085580"},{"id":"25#31#gene936581#97#diseaseC0003850","pred":"associated_with","subj":"20005218-1#25#31#gene9365","obj":"20005218-1#81#97#diseaseC0003850"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}

{"project":"tmVarCorpus","denotations":[{"id":"T1","span":{"begin":302,"end":308},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T2","span":{"begin":471,"end":477},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T3","span":{"begin":634,"end":637},"obj":"DNAMutation:|SUB|G||A"},{"id":"T4","span":{"begin":658,"end":664},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T5","span":{"begin":814,"end":820},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T6","span":{"begin":1009,"end":1015},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T7","span":{"begin":1042,"end":1048},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T8","span":{"begin":1481,"end":1487},"obj":"DNAMutation:c|SUB|G|-395|A"},{"id":"T9","span":{"begin":1603,"end":1609},"obj":"DNAMutation:c|SUB|G|-395|A"}],"text":"A potential regulatory single nucleotide polymorphism in the promoter of the Klotho gene may be associated with essential hypertension in the Chinese Han population.\nBACKGROUND: Mice with defects in the Klotho gene exhibit multiple aging phenotypes including arteriosclerosis. We hypothesised that the G-395A polymorphism in the promoter region of the human Klotho gene may contribute to the prevalence of Essential Hypertension (EH).\nMETHODS: We investigate whether the G-395A polymorphism of Klotho is associated with EH in a population consisting of 215 patients with EH and 220 non-hypertensive subjects. We also tested whether a G/A substitution at the G-395A site affected the transcription level in vitro through the dual-luciferase reporter assay.\nRESULTS: Differences in the genotype distributions of the G-395A polymorphism between the EH and non-hypertension groups are statistically significant (P=0.032). There are differential effects of age, gender and smoking status on the association of the G-395A polymorphism with EH; the G-395A polymorphism is significantly associated with EH in subjects over 60years old, in females and in nonsmokers. A multiple logistic regression analysis indicated that the odds ratio for EH in the -395A allele carriers as compared with the control group was 0.593 (P=0.024) after adjusting for current traditional risk factors. The dual-luciferase reporter assay revealed that the -395A carrier of a 498-bp DNA fragment (containing the G-395A site) upstream of the Klotho gene has higher relative luciferase activity than the -395G carrier.\nCONCLUSIONS: The G-395A polymorphism of the human Klotho gene is associated with EH and may be a potential regulatory site."}