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PubMed:18843533 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-149 Sentence denotes Adhesion forces in the self-recognition of oligosaccharide epitopes of the proteoglycan aggregation factor of the marine sponge Microciona prolifera.
TextSentencer_T2 150-285 Sentence denotes Cell aggregation in the marine sponge Microciona prolifera is mediated by a multimillion molecular-mass aggregation factor, termed MAF.
TextSentencer_T3 286-505 Sentence denotes Earlier investigations revealed that the cell aggregation activity of MAF depends on two functional domains: (i) a Ca(2+)-independent cell-binding domain and (ii) a Ca(2+)-dependent proteoglycan self-interaction domain.
TextSentencer_T4 506-784 Sentence denotes Structural analysis of involved carbohydrate fragments of the proteoglycan in the self-association established a sulfated disaccharide beta-D: -GlcpNAc3S-(1-->3)-alpha-L: -Fucp and a pyruvated trisaccharide beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp.
TextSentencer_T5 785-997 Sentence denotes Recent UV, SPR, and TEM studies, using BSA conjugates and gold nanoparticles of the synthetic sulfated disaccharide, clearly demonstrated self-recognition on the disaccharide level in the presence of Ca(2+)-ions.
TextSentencer_T6 998-1168 Sentence denotes To determine binding forces of the carbohydrate-carbohydrate interactions for both synthetic MAF oligosaccharides, atomic force microscopy (AFM) studies were carried out.
TextSentencer_T7 1169-1471 Sentence denotes It turned out that, in the presence of Ca(2+)-ions, the force required to separate the tip and sample coated with a self-assembling monolayer of thiol-spacer-containing beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- was found to be quantized in integer multiples of 30 +/- 6 pN.
TextSentencer_T8 1472-1553 Sentence denotes No binding was observed between the two monolayers in the absence of Ca(2+)-ions.
TextSentencer_T9 1554-1610 Sentence denotes Cd(2+)-ions could partially induce the self-interaction.
TextSentencer_T10 1611-1833 Sentence denotes In contrast, similar AFM experiments with thiol-spacer-containing beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- did not show a binding in the presence of Ca(2+)-ions.
TextSentencer_T11 1834-1983 Sentence denotes Also TEM experiments of gold nanoparticles coated with the pyruvated trisaccharide could not make visible aggregation in the presence of Ca(2+)-ions.
TextSentencer_T12 1984-2128 Sentence denotes It is suggested that the self-interaction between the sulfated disaccharide fragments is stronger than that between the pyruvated trisaccharide.
T1 0-149 Sentence denotes Adhesion forces in the self-recognition of oligosaccharide epitopes of the proteoglycan aggregation factor of the marine sponge Microciona prolifera.
T2 150-285 Sentence denotes Cell aggregation in the marine sponge Microciona prolifera is mediated by a multimillion molecular-mass aggregation factor, termed MAF.
T3 286-505 Sentence denotes Earlier investigations revealed that the cell aggregation activity of MAF depends on two functional domains: (i) a Ca(2+)-independent cell-binding domain and (ii) a Ca(2+)-dependent proteoglycan self-interaction domain.
T4 506-784 Sentence denotes Structural analysis of involved carbohydrate fragments of the proteoglycan in the self-association established a sulfated disaccharide beta-D: -GlcpNAc3S-(1-->3)-alpha-L: -Fucp and a pyruvated trisaccharide beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp.
T5 785-997 Sentence denotes Recent UV, SPR, and TEM studies, using BSA conjugates and gold nanoparticles of the synthetic sulfated disaccharide, clearly demonstrated self-recognition on the disaccharide level in the presence of Ca(2+)-ions.
T6 998-1168 Sentence denotes To determine binding forces of the carbohydrate-carbohydrate interactions for both synthetic MAF oligosaccharides, atomic force microscopy (AFM) studies were carried out.
T7 1169-1471 Sentence denotes It turned out that, in the presence of Ca(2+)-ions, the force required to separate the tip and sample coated with a self-assembling monolayer of thiol-spacer-containing beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- was found to be quantized in integer multiples of 30 +/- 6 pN.
T8 1472-1553 Sentence denotes No binding was observed between the two monolayers in the absence of Ca(2+)-ions.
T9 1554-1610 Sentence denotes Cd(2+)-ions could partially induce the self-interaction.
T10 1611-1833 Sentence denotes In contrast, similar AFM experiments with thiol-spacer-containing beta-D: -Galp4,6(R)Pyr-(1-->4)-beta-D: -GlcpNAc-(1-->3)-alpha-L: -Fucp-(1-->O)(CH(2))(3)S(CH(2))(6)S- did not show a binding in the presence of Ca(2+)-ions.
T11 1834-1983 Sentence denotes Also TEM experiments of gold nanoparticles coated with the pyruvated trisaccharide could not make visible aggregation in the presence of Ca(2+)-ions.
T12 1984-2128 Sentence denotes It is suggested that the self-interaction between the sulfated disaccharide fragments is stronger than that between the pyruvated trisaccharide.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 128-148 OrganismTaxon denotes Microciona prolifera 1190602
T2 188-208 OrganismTaxon denotes Microciona prolifera 1190602