| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-167 |
Sentence |
denotes |
Minimal disease monitoring by QRT-PCR: guidelines for identification and systematic validation of molecular markers prior to evaluation in prospective clinical trials. |
| TextSentencer_T2 |
168-347 |
Sentence |
denotes |
Real-time RT-PCR (QRT-PCR) is a sensitive method for the detection of minimal disease (MD) and may improve monitoring of disease status and stratification of patients for therapy. |
| TextSentencer_T3 |
348-495 |
Sentence |
denotes |
Where tumour-specific mRNAs have not been identified, the selection of which target(s) is(are) optimal for the detection of MD remains a challenge. |
| TextSentencer_T4 |
496-648 |
Sentence |
denotes |
This reflects the heterogeneity of tumour cells, the stability of mRNAs and low-level of transcription in cells of the normal haemopoietic compartments. |
| TextSentencer_T5 |
649-871 |
Sentence |
denotes |
The aim of this study was to establish for the first time guidelines for the systematic prioritization of potential markers of MD detected by QRT-PCR prior to evaluation in multicentre prospective clinical outcome studies. |
| TextSentencer_T6 |
872-1104 |
Sentence |
denotes |
We combined microarray analysis, ESTs gene expression profiles, improved probe-sets sequence annotation, and previously described standard operating procedures for QRT-PCR analysis to identify and prioritize potential markers of MD. |
| TextSentencer_T7 |
1105-1244 |
Sentence |
denotes |
Using this methodology, we identified 49 potential markers of MD in neuroblastoma (NB), of which 11 were associated with neuronal function. |
| TextSentencer_T8 |
1245-1365 |
Sentence |
denotes |
We found that, in addition to TH, Phox2B and DCX mRNA may be useful targets for the detection of MD in children with NB. |
| TextSentencer_T9 |
1366-1541 |
Sentence |
denotes |
This same strategy could be exploited to select MD markers of other solid tumours from the large number of potential targets identified by microarray gene expression profiles. |
| T1 |
0-167 |
Sentence |
denotes |
Minimal disease monitoring by QRT-PCR: guidelines for identification and systematic validation of molecular markers prior to evaluation in prospective clinical trials. |
| T2 |
168-347 |
Sentence |
denotes |
Real-time RT-PCR (QRT-PCR) is a sensitive method for the detection of minimal disease (MD) and may improve monitoring of disease status and stratification of patients for therapy. |
| T3 |
348-495 |
Sentence |
denotes |
Where tumour-specific mRNAs have not been identified, the selection of which target(s) is(are) optimal for the detection of MD remains a challenge. |
| T4 |
496-648 |
Sentence |
denotes |
This reflects the heterogeneity of tumour cells, the stability of mRNAs and low-level of transcription in cells of the normal haemopoietic compartments. |
| T5 |
649-871 |
Sentence |
denotes |
The aim of this study was to establish for the first time guidelines for the systematic prioritization of potential markers of MD detected by QRT-PCR prior to evaluation in multicentre prospective clinical outcome studies. |
| T6 |
872-1104 |
Sentence |
denotes |
We combined microarray analysis, ESTs gene expression profiles, improved probe-sets sequence annotation, and previously described standard operating procedures for QRT-PCR analysis to identify and prioritize potential markers of MD. |
| T7 |
1105-1244 |
Sentence |
denotes |
Using this methodology, we identified 49 potential markers of MD in neuroblastoma (NB), of which 11 were associated with neuronal function. |
| T8 |
1245-1365 |
Sentence |
denotes |
We found that, in addition to TH, Phox2B and DCX mRNA may be useful targets for the detection of MD in children with NB. |
| T9 |
1366-1541 |
Sentence |
denotes |
This same strategy could be exploited to select MD markers of other solid tumours from the large number of potential targets identified by microarray gene expression profiles. |
