PubMed:17967886 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":607,"end":610},"obj":"CellLine"},{"id":"T2","span":{"begin":621,"end":624},"obj":"CellLine"},{"id":"T3","span":{"begin":747,"end":750},"obj":"CellLine"},{"id":"T4","span":{"begin":857,"end":860},"obj":"CellLine"},{"id":"T5","span":{"begin":1043,"end":1046},"obj":"CellLine"},{"id":"T6","span":{"begin":1095,"end":1098},"obj":"CellLine"},{"id":"T7","span":{"begin":1250,"end":1253},"obj":"CellLine"}],"attributes":[{"id":"A1","pred":"cellosaurus_accession_id","subj":"T1","obj":"CVCL_8949"},{"id":"A2","pred":"cellosaurus_accession_id","subj":"T2","obj":"CVCL_8950"},{"id":"A3","pred":"cellosaurus_accession_id","subj":"T3","obj":"CVCL_8949"},{"id":"A4","pred":"cellosaurus_accession_id","subj":"T4","obj":"CVCL_8949"},{"id":"A5","pred":"cellosaurus_accession_id","subj":"T5","obj":"CVCL_4892"},{"id":"A6","pred":"cellosaurus_accession_id","subj":"T6","obj":"CVCL_8949"},{"id":"A7","pred":"cellosaurus_accession_id","subj":"T7","obj":"CVCL_4892"}],"text":"Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.\nThe Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 of Bmdsx pre-mRNA are specifically excluded in males. To explore how this occurs, we developed a novel in vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series of mutation analyses using a Bmdsx minigene with this in vivo splicing assay system identified three distinct sequences (CE1, CE2, and CE3) positioned in exon 4 as exonic splicing silencers responsible for male-specific splicing. Gel shift analysis showed that CE1 binds to a nuclear protein from male cells but not that from female cells. Mutation of UAA repeats within CE1 inhibited the binding of the nuclear protein to the RNA and caused female-specific splicing in male cells. We have identified BmPSI, a Bombyx homolog of P-element somatic inhibitor (PSI), as the nuclear factor that specifically binds CE1. Down-regulation of endogenous BmPSI by RNA interference significantly increased female-specific splicing in male cells. This is the first report of a PSI homolog implicated in the regulated sex-specific splicing of dsx pre-mRNA."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":140,"end":151},"obj":"Species:7091"},{"id":"T2","span":{"begin":174,"end":185},"obj":"Species:7091"},{"id":"T3","span":{"begin":208,"end":213},"obj":"Gene:692415"},{"id":"T4","span":{"begin":242,"end":250},"obj":"Species:7091"},{"id":"T5","span":{"begin":288,"end":293},"obj":"Gene:692415"},{"id":"T6","span":{"begin":515,"end":520},"obj":"Gene:692415"},{"id":"T7","span":{"begin":987,"end":992},"obj":"Gene:100126539"},{"id":"T8","span":{"begin":1043,"end":1046},"obj":"Gene:100126539"},{"id":"T9","span":{"begin":1130,"end":1135},"obj":"Gene:100126539"},{"id":"T10","span":{"begin":1250,"end":1253},"obj":"Gene:100126539"}],"text":"Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.\nThe Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 of Bmdsx pre-mRNA are specifically excluded in males. To explore how this occurs, we developed a novel in vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series of mutation analyses using a Bmdsx minigene with this in vivo splicing assay system identified three distinct sequences (CE1, CE2, and CE3) positioned in exon 4 as exonic splicing silencers responsible for male-specific splicing. Gel shift analysis showed that CE1 binds to a nuclear protein from male cells but not that from female cells. Mutation of UAA repeats within CE1 inhibited the binding of the nuclear protein to the RNA and caused female-specific splicing in male cells. We have identified BmPSI, a Bombyx homolog of P-element somatic inhibitor (PSI), as the nuclear factor that specifically binds CE1. Down-regulation of endogenous BmPSI by RNA interference significantly increased female-specific splicing in male cells. This is the first report of a PSI homolog implicated in the regulated sex-specific splicing of dsx pre-mRNA."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":140,"end":151},"obj":"Species:7091"},{"id":"T10","span":{"begin":1250,"end":1253},"obj":"Gene:100126539"},{"id":"T2","span":{"begin":174,"end":185},"obj":"Species:7091"},{"id":"T3","span":{"begin":208,"end":213},"obj":"Gene:692415"},{"id":"T4","span":{"begin":242,"end":250},"obj":"Species:7091"},{"id":"T5","span":{"begin":288,"end":293},"obj":"Gene:692415"},{"id":"T6","span":{"begin":515,"end":520},"obj":"Gene:692415"},{"id":"T7","span":{"begin":987,"end":992},"obj":"Gene:100126539"},{"id":"T8","span":{"begin":1043,"end":1046},"obj":"Gene:100126539"},{"id":"T9","span":{"begin":1130,"end":1135},"obj":"Gene:100126539"}],"text":"Establishment of a novel in vivo sex-specific splicing assay system to identify a trans-acting factor that negatively regulates splicing of Bombyx mori dsx female exons.\nThe Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 of Bmdsx pre-mRNA are specifically excluded in males. To explore how this occurs, we developed a novel in vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series of mutation analyses using a Bmdsx minigene with this in vivo splicing assay system identified three distinct sequences (CE1, CE2, and CE3) positioned in exon 4 as exonic splicing silencers responsible for male-specific splicing. Gel shift analysis showed that CE1 binds to a nuclear protein from male cells but not that from female cells. Mutation of UAA repeats within CE1 inhibited the binding of the nuclear protein to the RNA and caused female-specific splicing in male cells. We have identified BmPSI, a Bombyx homolog of P-element somatic inhibitor (PSI), as the nuclear factor that specifically binds CE1. Down-regulation of endogenous BmPSI by RNA interference significantly increased female-specific splicing in male cells. This is the first report of a PSI homolog implicated in the regulated sex-specific splicing of dsx pre-mRNA."}