PubMed:17426117 JSONTXT

{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":50,"end":59},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":77,"end":92},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":192,"end":208},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":247,"end":263},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":364,"end":379},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":434,"end":443},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":444,"end":459},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":1182,"end":1187},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":1322,"end":1337},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":1365,"end":1368},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":1426,"end":1442},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0000605"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0008315"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0008315"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0008315"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0008315"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0000605"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0008315"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0005070"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0008315"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"0012833"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"0008315"}],"text":"A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells.\nAlthough the vital role of the androgen receptor (AR) has been well demonstrated in primary prostate cancers, its role in the androgen-insensitive prostate cancers still remains unclear. Here, we used a small hairpin RNA approach to directly assess AR activity in prostate cancer cells. Reduction of AR expression in the two androgen-sensitive prostate cancer cell lines, LNCaP and LAPC4, significantly decreased AR-mediated transcription and cell growth. Intriguingly, in two androgen-insensitive prostate cell lines, LNCaP-C42B4 and CWR22Rv1, knockdown of AR expression showed a more pronounced effect on AR-induced transcription and cell growth than androgen depletion. Using cDNA microarrays, we also compared the transcriptional profiles induced by either androgen depletion or AR knockdown. Although a significant number of transcripts appear to be regulated by both androgen depletion and AR knockdown, we observed a subset of transcripts affected only by androgen depletion but not by AR knockdown, and vice versa. Finally, we demonstrated a direct role for AR in promoting tumor formation and growth in a xenograft model. Taken together, our results elucidate an important role for the AR in androgen-insensitive prostate cancer cells, and suggest that AR can be used as a therapeutic target for androgen-insensitive prostate cancers."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":192,"end":208},"obj":"HP_0012125"},{"id":"T2","span":{"begin":201,"end":208},"obj":"HP_0002664"},{"id":"T3","span":{"begin":247,"end":263},"obj":"HP_0012125"},{"id":"T4","span":{"begin":256,"end":263},"obj":"HP_0002664"},{"id":"T5","span":{"begin":364,"end":379},"obj":"HP_0012125"},{"id":"T6","span":{"begin":373,"end":379},"obj":"HP_0002664"},{"id":"T7","span":{"begin":444,"end":459},"obj":"HP_0012125"},{"id":"T8","span":{"begin":453,"end":459},"obj":"HP_0002664"},{"id":"T9","span":{"begin":1182,"end":1187},"obj":"HP_0002664"},{"id":"T10","span":{"begin":1322,"end":1337},"obj":"HP_0012125"},{"id":"T11","span":{"begin":1331,"end":1337},"obj":"HP_0002664"},{"id":"T12","span":{"begin":1426,"end":1442},"obj":"HP_0012125"},{"id":"T13","span":{"begin":1435,"end":1442},"obj":"HP_0002664"},{"id":"T1","span":{"begin":192,"end":208},"obj":"HP_0012125"},{"id":"T2","span":{"begin":201,"end":208},"obj":"HP_0002664"},{"id":"T3","span":{"begin":247,"end":263},"obj":"HP_0012125"},{"id":"T4","span":{"begin":256,"end":263},"obj":"HP_0002664"},{"id":"T5","span":{"begin":364,"end":379},"obj":"HP_0012125"},{"id":"T6","span":{"begin":373,"end":379},"obj":"HP_0002664"},{"id":"T7","span":{"begin":444,"end":459},"obj":"HP_0012125"},{"id":"T8","span":{"begin":453,"end":459},"obj":"HP_0002664"},{"id":"T9","span":{"begin":1182,"end":1187},"obj":"HP_0002664"},{"id":"T10","span":{"begin":1322,"end":1337},"obj":"HP_0012125"},{"id":"T11","span":{"begin":1331,"end":1337},"obj":"HP_0002664"},{"id":"T12","span":{"begin":1426,"end":1442},"obj":"HP_0012125"},{"id":"T13","span":{"begin":1435,"end":1442},"obj":"HP_0002664"}],"text":"A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells.\nAlthough the vital role of the androgen receptor (AR) has been well demonstrated in primary prostate cancers, its role in the androgen-insensitive prostate cancers still remains unclear. Here, we used a small hairpin RNA approach to directly assess AR activity in prostate cancer cells. Reduction of AR expression in the two androgen-sensitive prostate cancer cell lines, LNCaP and LAPC4, significantly decreased AR-mediated transcription and cell growth. Intriguingly, in two androgen-insensitive prostate cell lines, LNCaP-C42B4 and CWR22Rv1, knockdown of AR expression showed a more pronounced effect on AR-induced transcription and cell growth than androgen depletion. Using cDNA microarrays, we also compared the transcriptional profiles induced by either androgen depletion or AR knockdown. Although a significant number of transcripts appear to be regulated by both androgen depletion and AR knockdown, we observed a subset of transcripts affected only by androgen depletion but not by AR knockdown, and vice versa. Finally, we demonstrated a direct role for AR in promoting tumor formation and growth in a xenograft model. Taken together, our results elucidate an important role for the AR in androgen-insensitive prostate cancer cells, and suggest that AR can be used as a therapeutic target for androgen-insensitive prostate cancers."}

{"project":"CoMAGC","denotations":[{"id":"T1","span":{"begin":400,"end":402},"obj":"Gene"},{"id":"E1","span":{"begin":403,"end":413},"obj":"Gene_expression"},{"id":"E2","span":{"begin":387,"end":396},"obj":"Negative_regulation"},{"id":"T2","span":{"begin":444,"end":459},"obj":"prostate cancer"},{"id":"T3","span":{"begin":472,"end":477},"obj":"prostate cancer"},{"id":"T4","span":{"begin":482,"end":487},"obj":"prostate cancer"}],"relations":[{"id":"R1","pred":"themeOf","subj":"T1","obj":"E1"},{"id":"R2","pred":"themeOf","subj":"E1","obj":"E2"},{"id":"R3","pred":"CGE-decreased","subj":"T1","obj":"T2"},{"id":"R4","pred":"CCS-cancerTOnormal","subj":"T1","obj":"T2"},{"id":"R5","pred":"PT-causality","subj":"T1","obj":"T2"},{"id":"R3","pred":"IGE-unidentifiable","subj":"T1","obj":"T2"}],"text":"A promoting role of androgen receptor in androgen-sensitive and -insensitive prostate cancer cells.\nAlthough the vital role of the androgen receptor (AR) has been well demonstrated in primary prostate cancers, its role in the androgen-insensitive prostate cancers still remains unclear. Here, we used a small hairpin RNA approach to directly assess AR activity in prostate cancer cells. Reduction of AR expression in the two androgen-sensitive prostate cancer cell lines, LNCaP and LAPC4, significantly decreased AR-mediated transcription and cell growth. Intriguingly, in two androgen-insensitive prostate cell lines, LNCaP-C42B4 and CWR22Rv1, knockdown of AR expression showed a more pronounced effect on AR-induced transcription and cell growth than androgen depletion. Using cDNA microarrays, we also compared the transcriptional profiles induced by either androgen depletion or AR knockdown. Although a significant number of transcripts appear to be regulated by both androgen depletion and AR knockdown, we observed a subset of transcripts affected only by androgen depletion but not by AR knockdown, and vice versa. Finally, we demonstrated a direct role for AR in promoting tumor formation and growth in a xenograft model. Taken together, our results elucidate an important role for the AR in androgen-insensitive prostate cancer cells, and suggest that AR can be used as a therapeutic target for androgen-insensitive prostate cancers."}