PubMed:17028180 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/17028180","sourcedb":"PubMed","sourceid":"17028180","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/17028180","text":"Transcriptional regulation by Foxp3 is associated with direct promoter occupancy and modulation of histone acetylation.\nRegulatory T cells (T(reg)) express Foxp3, a forkhead family member that is necessary and sufficient for T(reg) lineage choice and function. Ectopic expression of Foxp3 in non-T(reg) leads to repression of the interleukin 2 (IL-2) and interferon gamma (IFNgamma) genes, gain of suppressor function, and induction of genes such as CD25, GITR, and CTLA-4, but the mode by which Foxp3 enforces this program is unclear. Using chromatin immunoprecipitation, we have demonstrated that Foxp3 binds to the endogenous IL-2 and IFNgamma loci in T cells, but only after T cell receptor stimulation. This activation-induced Foxp3 binding was abrogated by cyclosporin A, suggesting a role for the phosphatase calcineurin in Foxp3 function. We have also shown that binding of Foxp3 to the IL-2 and IFNgamma genes induces active deacetylation of histone H3, a process that inhibits chromatin remodeling and opposes gene transcription. Conversely, binding of Foxp3 to the GITR, CD25, and CTLA-4 genes results in increased histone acetylation. These data indicate that Foxp3 may regulate transcription through direct chromatin remodeling and show that Foxp3 function is influenced by signals from the TCR.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":119},"obj":"Sentence"},{"id":"T2","span":{"begin":120,"end":260},"obj":"Sentence"},{"id":"T3","span":{"begin":261,"end":535},"obj":"Sentence"},{"id":"T4","span":{"begin":536,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":846},"obj":"Sentence"},{"id":"T6","span":{"begin":847,"end":1039},"obj":"Sentence"},{"id":"T7","span":{"begin":1040,"end":1146},"obj":"Sentence"},{"id":"T8","span":{"begin":1147,"end":1308},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":30,"end":35},"obj":"Protein"},{"id":"T2","span":{"begin":99,"end":106},"obj":"Protein"},{"id":"T3","span":{"begin":156,"end":161},"obj":"Protein"},{"id":"T4","span":{"begin":283,"end":288},"obj":"Protein"},{"id":"T5","span":{"begin":330,"end":343},"obj":"Protein"},{"id":"T6","span":{"begin":345,"end":349},"obj":"Protein"},{"id":"T7","span":{"begin":355,"end":371},"obj":"Protein"},{"id":"T8","span":{"begin":373,"end":381},"obj":"Protein"},{"id":"T9","span":{"begin":450,"end":454},"obj":"Protein"},{"id":"T10","span":{"begin":456,"end":460},"obj":"Protein"},{"id":"T11","span":{"begin":466,"end":472},"obj":"Protein"},{"id":"T12","span":{"begin":496,"end":501},"obj":"Protein"},{"id":"T13","span":{"begin":599,"end":604},"obj":"Protein"},{"id":"T14","span":{"begin":629,"end":633},"obj":"Protein"},{"id":"T15","span":{"begin":638,"end":646},"obj":"Protein"},{"id":"T16","span":{"begin":732,"end":737},"obj":"Protein"},{"id":"T17","span":{"begin":831,"end":836},"obj":"Protein"},{"id":"T18","span":{"begin":882,"end":887},"obj":"Protein"},{"id":"T19","span":{"begin":895,"end":899},"obj":"Protein"},{"id":"T20","span":{"begin":904,"end":912},"obj":"Protein"},{"id":"T21","span":{"begin":951,"end":961},"obj":"Protein"},{"id":"T22","span":{"begin":1063,"end":1068},"obj":"Protein"},{"id":"T23","span":{"begin":1076,"end":1080},"obj":"Protein"},{"id":"T24","span":{"begin":1082,"end":1086},"obj":"Protein"},{"id":"T25","span":{"begin":1092,"end":1098},"obj":"Protein"},{"id":"T26","span":{"begin":1126,"end":1133},"obj":"Protein"},{"id":"T27","span":{"begin":1172,"end":1177},"obj":"Protein"},{"id":"T28","span":{"begin":1255,"end":1260},"obj":"Protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T11","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T12","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T13","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T14","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T15","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T16","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T17","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T18","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T19","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T20","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T21","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T22","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T23","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T24","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T25","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T26","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T27","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T28","pred":"source","obj":"bionlp-st-epi-2011-training"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":679,"end":685},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":120,"end":138},"obj":"Cell"},{"id":"T2","span":{"begin":140,"end":146},"obj":"Cell"},{"id":"T4","span":{"begin":225,"end":231},"obj":"Cell"},{"id":"T6","span":{"begin":296,"end":302},"obj":"Cell"},{"id":"T8","span":{"begin":655,"end":662},"obj":"Cell"},{"id":"T9","span":{"begin":679,"end":685},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000815"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000792"},{"id":"A3","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000815"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000792"},{"id":"A5","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000815"},{"id":"A6","pred":"cl_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL:0000792"},{"id":"A7","pred":"cl_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL:0000815"},{"id":"A8","pred":"cl_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"subj":"T1","pred":"source","obj":"CL-cell"},{"subj":"T2","pred":"source","obj":"CL-cell"},{"subj":"T4","pred":"source","obj":"CL-cell"},{"subj":"T6","pred":"source","obj":"CL-cell"},{"subj":"T8","pred":"source","obj":"CL-cell"},{"subj":"T9","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93c5ec","default":true},{"id":"bionlp-st-epi-2011-training","color":"#dfec93"},{"id":"Anatomy-UBERON","color":"#df93ec"},{"id":"CL-cell","color":"#93ecc5"}]}]}}